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All Journal Biodiversitas Journal of Biological Diversity
WIWIEK TYASNINGSIH
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Mulyorejo, Kampus C FKH-UNAIR, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; WIWIEK TYASNINGSIH; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; CHARITY CHINYERE NNABUGWU; DIVINEGIFT OLUCHI OKATA-NWALI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240965

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Abstract. Ugbo EN, Effendi MH, Witaningrum AM, Tyasningsih W, Agumah BN, Ugbo AI, Nnabugwu CC, Okata-Nwali DO. 2023. Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. Biodiversitas 24: 5207-5214. Salmonella is a zoonotic foodborne pathogen that can cause serious illness in humans and animals worldwide. Antimicrobial resistance caused by microorganisms has greatly challenged veterinary and human medicine regarding disease treatments. The objective of this study was to evaluate the antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. One hundred eighty samples (90 broiler feces; 90 broiler cloacal swabs) were collected from three (3) poultry farms in the Abakaliki metropolis. Samples were analyzed and identified for the presence of Salmonella isolates using standard microbiological and biochemical analysis. The antimicrobial sensitivity test of Salmonella isolates against the selected antibiotics was done using the Kirby-Bauer disk diffusion method on Mueller Hinton agar. The results showed 61 samples (33.9 %) were positive for Salmonella. Feces and cloacal swab samples positive for Salmonella were 46.7% and 21.1%, respectively. Multidrug resistance Salmonella (MDR) strains had an overall prevalence of samples (16.2%), i.e., 7 samples of broiler feces had 7 (16.7%) and 3 samples of cloacal swab (15.8%). Thus, all the MDR Salmonella isolates were 100% resistant to tetracycline and ampicillin. The multiple antibiotic-resistant indexes of the MDR were 0.51. This study indicated that broilers chicken and their products were potential sources of human salmonellosis and sources for transmitting MDR Salmonella in Abakaliki, Nigeria. Poultry farmers should use antibiotics in appropriate dosages in their poultry farms.
Antimicrobial resistance in Campylobacter species isolated from commercially sold steak (beef) and raw cow milk in Abakaliki, Nigeria NNABUIFE BERNARD AGUMAH; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO; CHINEKWU SHERRIDAN NWAGWU; IFUNANYA AGATHA UGBO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250306

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Abstract. Agumah NB, Effendi MH, Witaningrum AM, Tyasningsih W, Ugbo EN, Nwagwu CS, Ugbo IA. 2024. Antimicrobial resistance in Campylobacter species isolated from commercially sold steak (beef) and raw cow milk in Abakaliki, Nigeria. Biodiversitas 25: 950-956. This study aimed to ascertain antimicrobial resistance in Campylobacter species isolated from commercially sold steak (Beef) and raw cow milk in Abakaliki. Pools of milk samples and steak were bought at various outlets and analyzed using standard microbiological methods. Results showed that two species of Campylobacter, namely Campylobacter coli, were isolated from the samples. In raw cow milk, Campylobacter coli had a frequency of 65% higher than Campylobacter jejuni (20%). While in steak, C. coli was 34.53% higher than C. jejuni (19.10%). The antibiotic susceptibility profile for C. coli isolated from raw cow milk ranged from 61.54% to 100%, especially for trimethoprim and cephalexin. Resistance ranged from 0.00 to 38.46%. The highest resistance was recorded for tetracycline. The antibiotic susceptibility profile for C. coli isolated from steak recorded susceptibility values ranging from 60.53% to 86.84%. Cephalexin had the highest effect on C. coli isolates from steak. Resistance values ranging from 13.16% to 42.11% were recorded with Nalidixic acid as the least effective antibiotic. C. jejuni isolated from cow raw milk, susceptibility ranging from 0.00% to 100% was recorded, especially for Cefotaxime, Imipenem, Cephalexin, and Nalidixic acid. Resistance ranged from 0.00% to 100%, especially for Chloramphenicol and Kanamycin. For C. jejuni, susceptibility values ranged from 26.57% to 85.71%, with imipenem presenting the highest effect. Resistance values ranged from 23.81% to 71.43%. Gentamycin had the least effect. Adequate sanitation, personal hygiene, and appropriate use of antibiotics are highly recommended for food handlers and farmers to safeguard human health by reducing AMR challenges, even in milk and meat/meat products.
Co-Authors ADIANA MUTAMSARI WITANINGRUM ADIANA MUTAMSARI WITANINGRUM AGATHA IFUNANYA UGBO BERNARD NNABUIFE AGUMAH CHARITY CHINYERE NNABUGWU CHINEKWU SHERRIDAN NWAGWU DIVINEGIFT OLUCHI OKATA-NWALI EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO IFUNANYA AGATHA UGBO MUSTOFA HELMI EFFENDI MUSTOFA HELMI EFFENDI NNABUIFE BERNARD AGUMAH