EMMANUEL NNABUIKE UGBO
Department of Applied Microbiology, Faculty of Science, Ebonyi State University. Enugu - Abakaliki Rd, 481101, Abakaliki, Ebonyi, Nigeria

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Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; JENNIFER INIOBONG JACOB; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; BENJAMIN IKECHUKWU MOSES
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240939

Abstract

Abstract. Ugbo EN, Jacob JI, Effendi MH, Witaningrum AM, Agumah BN, Ugbo AI, Moses BI. 2023. Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria. Biodiversitas 24: 4960-4966. The emergence of antimicrobial and multi-resistance extended-spectrum beta-lactamase-producing organisms (ESBL) from the environment and hospitals is a serious global health concern. This research focused on the role of poultry slaughterhouse wastewater as a reservoir for spreading extended-spectrum beta-lactamase-producing Escherichia coli. Exactly 110 poultry slaughterhouses wastewater was collected using sterile universal containers from abattoirs in Abakaliki. The samples were analyzed microbiological for the presence of E. coli using standard techniques. The E. coli isolates found were subjected to a sensitivity test to antibiotics using the Kirby-Bauer disc diffusion method and further tested for ESBL-producing E. coli using a double disc synergy test and chromogenic media Brilliance ESBL agar. Of 110 wastewater samples analyzed, 55 (50.0%) were positive for E. coli contamination. The E. coli isolates showed antibiotic resistance ranging between 21.8% (amoxicillin clavulanic acid) to 69.1% (tetracycline), and good susceptibility was observed on cefepime (72.7%). Thus, among 55 E. coli isolates, 11(20.0%) were ESBL-producing E. coli with resistance patterns as follows: TET-STX-CAZ-CTX-FEP (four isolates); TET-STX-CAZ-CTX (four isolates); TET-STX-CAZ-CTX-FEP-CRO (three isolates), and average multidrug resistance index of 0.7. This study revealed that poultry slaughterhouse wastewater was a reservoir for multidrug resistance ESBL-producing E. coli. Therefore, properly treating wastewater from poultry production or farms before disposing into the drainage channel or water bodies is highly recommended to avoid spreading ESBL-producing organisms from animal waste products to humans and their environment.
Antimicrobial resistance in Campylobacter species isolated from commercially sold steak (beef) and raw cow milk in Abakaliki, Nigeria NNABUIFE BERNARD AGUMAH; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO; CHINEKWU SHERRIDAN NWAGWU; IFUNANYA AGATHA UGBO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250306

Abstract

Abstract. Agumah NB, Effendi MH, Witaningrum AM, Tyasningsih W, Ugbo EN, Nwagwu CS, Ugbo IA. 2024. Antimicrobial resistance in Campylobacter species isolated from commercially sold steak (beef) and raw cow milk in Abakaliki, Nigeria. Biodiversitas 25: 950-956. This study aimed to ascertain antimicrobial resistance in Campylobacter species isolated from commercially sold steak (Beef) and raw cow milk in Abakaliki. Pools of milk samples and steak were bought at various outlets and analyzed using standard microbiological methods. Results showed that two species of Campylobacter, namely Campylobacter coli, were isolated from the samples. In raw cow milk, Campylobacter coli had a frequency of 65% higher than Campylobacter jejuni (20%). While in steak, C. coli was 34.53% higher than C. jejuni (19.10%). The antibiotic susceptibility profile for C. coli isolated from raw cow milk ranged from 61.54% to 100%, especially for trimethoprim and cephalexin. Resistance ranged from 0.00 to 38.46%. The highest resistance was recorded for tetracycline. The antibiotic susceptibility profile for C. coli isolated from steak recorded susceptibility values ranging from 60.53% to 86.84%. Cephalexin had the highest effect on C. coli isolates from steak. Resistance values ranging from 13.16% to 42.11% were recorded with Nalidixic acid as the least effective antibiotic. C. jejuni isolated from cow raw milk, susceptibility ranging from 0.00% to 100% was recorded, especially for Cefotaxime, Imipenem, Cephalexin, and Nalidixic acid. Resistance ranged from 0.00% to 100%, especially for Chloramphenicol and Kanamycin. For C. jejuni, susceptibility values ranged from 26.57% to 85.71%, with imipenem presenting the highest effect. Resistance values ranged from 23.81% to 71.43%. Gentamycin had the least effect. Adequate sanitation, personal hygiene, and appropriate use of antibiotics are highly recommended for food handlers and farmers to safeguard human health by reducing AMR challenges, even in milk and meat/meat products.