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MIRNI LAMID
Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia AGUS WIDODO; MIRNI LAMID; MUSTOFA HELMI EFFENDI; ASWIN RAFIF KHAILRULLAH; SHENDY CANADYA KURNIAWAN; OTTO SAHAT MARTUA SILAEN; KATTY HENDRIANA PRISCILIA RIWU; LITA RAKHMA YUSTINASARI; DANIAH ASHRI AFNANI; FIDI NUR AINI EKA PUJI DAMEANTI; SANCAKA CHASYER RAMANDINIANTO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240126

Abstract

Abstract. Widodo A, Lamid M, Effendi MH, Khairullah AR, Kurniawan SC, Silaen OSM, Riwu KHP, Yustinasari LR, Afnani DA, Dameanti FNAEP, Ramandinianto SC. 2022. Antimicrobial resistance characteristics of multidrug resistance and extended spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia. Biodiversitas 23: 215-221. Escherichia coli bacteria initially reside in the digestive tract of humans and animals but are able to adapt to new environments that are different from their initial habitat. The pathogenicity of E. coli can occur when these bacteria grow more than normal limits, produce toxins, and are resistant to certain types of antibiotics. The purpose of this study was to investigate the antimicrobial resistance characteristics of MDR and ESBL-producing E. coli from several dairy farms in the Probolinggo district of East Java province, Indonesia. A total of 150 samples consisting of 109 milk and 41 environmental samples from 41 dairy farms were used for isolation. TSIA and IMViC biochemical tests were used to identify E. coli bacteria. Escherichia coli resistance profile was obtained through disc diffusion test on several antibiotics, namely tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Escherichia coli that was resistant to 3 or more antibiotics was defined as MDR. The results of isolation and identification obtained 124 (82.6%) isolates characterizing E. coli bacteria. The antimicrobial susceptibility test of E. coli showed 9 (7.26%) MDR isolates and 2 (22.22%) ESBL isolates by double-disc synergy test (DDST). MDR E. coli was dominated by the pattern of antimicrobial drug resistance TE-S-W (tetracycline, streptomycin, trimethoprim) with a total of 8 (38.10%) isolates, followed by antimicrobial drug resistance pattern TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) with one (4.76%) E. coli isolates. The pattern of antimicrobial drugs of ESBL E. coli showed in one (11.11%) sample of ESBL E. coli from a milk sample with the pattern of TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) and one (11.11%) sample ESBL E. coli (AL 30) from the environmental sample with a pattern of TE-S-W pattern (tetracycline, streptomycin, trimethoprim). The discovery of MDR E. coli isolates and ESBL E. coli from milk and environmental samples at several dairy farms in Probolinggo district, East Java, Indonesia is a matter of concern and requires real action to reduce antibiotic resistance.
Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia AGUS WIDODO; MIRNI LAMID; MUSTOFA HELMI EFFENDI; WIWIEK TYASNINGSIH; WURLINA WURLINA; MOHAMMAD ANAM AL-ARIF; DADIK RAHARJO; SOEHARSONO SOEHARSONO; ASWIN RAFIF KHAIRULLAH; KATTY HENDRIANA PRISCILIA RIWU; LITA RAKHMA YUSTINASARI; SHENDY CANADYA KURNIAWAN; OTTO SAHAT MARTUA SILAEN; MOSES IKECHUKWU BENJAMIN; DANIAH ASHRI AFNANI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 3 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240367

Abstract

Abstract. Widodo A, Lamid M, Effendi MH, Tyasningsih W, Wurlina, Al-Arif MA, Raharjo D, Soeharsono, Khairullah AF, Riwu KHP, Yustinasari LR, Kurniawan SC, Silaen OSM, Benjamin MI, Afnani DA. 2023. Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia. Biodiversitas 24: 1900-1907. Escherichia coli bacteria initially reside in the digestive tract of humans and animals. This bacterium is treated with antibiotics, but this treatment may trigger antibiotic resistance. Antibiotic resistance genes found in E. coli can transfer to other bacteria, especially in the case of the emergence of multidrug resistance (MDR) bacteria. E. coli is a significant environmental contaminant on dairy farms and is frequently linked to genes that encode extended-spectrum beta-lactamase. This research focused on investigating the potential hazards of multidrug resistance E. coli collected from wastewater on dairy farms in East Java, Indonesia. The number of research samples used was 139 wastewater samples collected from 125 dairy farms in East Java, Indonesia. First, wastewater samples from dairy farms were isolated into BGLB media, then continued with culture on EMBA media, and identifying E. coli bacteria with TSIA and IMViC biochemical tests. The resistance patterns of E. coli were obtained through disk diffusion antibiotic tests on tetracycline, streptomycin, trimethoprim, chloramphenicol, and aztreonam. The results of the isolation and identification obtained 122 (87.77%) E. coli isolates. Antimicrobial susceptibility test to E. coli isolates showed 14 (11.47%) MDR E. coli isolates. The antimicrobial drug resistance pattern TE-ST-W (tetracycline, streptomycin, trimethoprim) accounted for most MDR E. coli isolates in this study, comprising 10 (36.26%); followed by TE-ST-C (tetracycline, streptomycin, chloramphenicol) with two (6.54%) MDR E. coli and two (6.54%) MDR E.coli isolates were resistant to four antibiotics, with the pattern of resistance to TE-ST-W-C (tetracycline, streptomycin, trimethoprim, chloramphenicol) antibiotic. The discovery of multidrug-resistant E. coli isolated from wastewater on dairy farms in East Java, Indonesia, is a new potential hazard that affects public health and needs solutions. One Health integration is anticipated to be used as a solution.
Co-Authors AGUS WIDODO ASWIN RAFIF KHAILRULLAH ASWIN RAFIF KHAIRULLAH DADIK RAHARJO DANIAH ASHRI AFNANI DANIAH ASHRI AFNANI FIDI NUR AINI EKA PUJI DAMEANTI KATTY HENDRIANA PRISCILIA RIWU LITA RAKHMA YUSTINASARI MOHAMMAD ANAM AL-ARIF MOSES IKECHUKWU BENJAMIN MUSTOFA HELMI EFFENDI OTTO SAHAT MARTUA SILAEN OTTO SAHAT MARTUA SILAEN SANCAKA CHASYER RAMANDINIANTO SHENDY CANADYA KURNIAWAN SHENDY CANADYA KURNIAWAN SOEHARSONO SOEHARSONO WIWIEK TYASNINGSIH WURLINA WURLINA