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All Journal Biodiversitas Journal of Biological Diversity
RICO RAMADHAN
Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antibacterial and biosurfactant activity of endophytic bacteria isolated from mangrove plant in Lamongan, Indonesia FATIMAH FATIMAH; NAZIL DWI RAHAYUNINGTYAS; ALIFAH NASTITI; DELA DWI ALAWIYAH; RICO RAMADHAN; ALMANDO GERALDI; JUNAIRIAH JUNAIRIAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250725

Abstract

Abstract. Fatimah, Rahayuningtyas ND, Nastiti A, Alawiyah DD, Ramadhan E, Geraldi A, Junairiah. 2024. Antibacterial and biosurfactant activity of endophytic bacteria isolated from mangrove plant in Lamongan, Indonesia. Biodiversitas 25: 3035-3042. This study aims to determine the antibacterial and biosurfactant activity of 61 endophytic bacteria from mangrove plants in Kutang Lamongan Beach. Antibacterial screening of supernatant of endophytic bacteria was performed using the disc diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. The most potent isolate was identified using 16s rRNA. The chemical compounds of the most potent isolate were identified using GC-MS analysis. The data were analyzed descriptively. Biosurfactant activity was also performed on the most potential isolate. All the isolates showed inhibitory zones against E. coli and S. aureus. Isolate LMG-2 produced the highest antimicrobial activity, averaging 9.57±0.4 mm against E. coli and 8.87±0.36 mm against S. aureus. The biosurfactant activity of LMG-2 isolate produced a surface tension of 39.09+0.49 mN/m and emulsification activity of 65.03+0.03% against kerosene, 47.63+0.10% against crude oil and 46.53+0.27 to diesel. The isolate LMG-2 had 98,41% similarity with the Bacillus amyloliquefaciens strain NBRC 15535 with a query cover of 100%. The biosurfactant extract contains 9-octadecenoic acid methyl ester, (E)-, 9,12-octadecadienoic acid (Z, Z) methyl ester, and 9,12,15-octadecadienoic acid methyl ester. These compounds, known as Fatty Acid Methyl Esters (FAMEs), were integral components of biosurfactants, surface-active agents produced by microorganisms.
Isolation and potential evaluation of organophosphate-indigenous degrading fungi from Singolangu Farmland, Magetan, Indonesia PUJIATI PUJIATI; HERTANTI HERTANTI; R. BEKTI KISWARDIANTA; FATIMAH FATIMAH; RICO RAMADHAN; NI'MATUZAHROH NI'MATUZAHROH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260118

Abstract

Abstract. Pujiati, Hertanti, Kiswardianta RB, Fatimah, Ramadhan R, Ni’matuzahroh. 2025. Isolation and potential evaluation of organophosphate-indigenous degrading fungi from Singolangu Farmland, Magetan, Indonesia. Biodiversitas 26: 157-168. Pesticide use is prevalent in Indonesian agriculture, especially in vegetable farming regions like Singolangu Hamlet, Sarangan Village, Plaosan, Magetan, East Java. While pesticides effectively control pests, their overuse and misuse have caused soil contamination, health disruptions, and decreased agricultural productivity. This study examines the potential of indigenous fungi from Singolangu to remediate soil polluted by organophosphate pesticides, specifically chlorpyrifos and profenofos. Twelve indigenous mold isolates were isolated and ex-situ bioaugmentation was performed with six treatments. The levels of N, P, K, pH, and organophosphate pesticide residues were analyzed. Aspergillus flavus was the most effective in bioremediation, achieving significant results within four weeks and displaying N, P, K, pH values of 101 ppm, 99 ppm, 306 ppm, and 6, respectively. The KPSK Formula (a mixture of all Fungi KPS1 to KPS12 ) which utilized a 12-isolate consortium, also showed significant results, with N, P, K, and pH values of 57 ppm, 59 ppm, 138 ppm, and 6, respectively. The control soil had 150 ppm of chlorpyrifos and 29 ppm of profenofos left over from the pesticides, but the bioremediation treatment KPS3 lowered these levels to 97 ppm of chlorpyrifos and 5.5 ppm of profenofos. This study indicates that indigenous fungi can efficiently degrade profenofos and chlorpyrifos pesticides, either individually or in consortium.
Bioprospecting of non-timber forest products of selected flora in Baluran National Park, East Java, Indonesia for phytochemicals, anti-diabetic, anti-tyrosinase, and antioxidants RICO RAMADHAN; NI’MATUZAHROH NI’MATUZAHROH; DINDA AYU LESTARI; NOVA ALFIAN HARIYANTO; SUHARTINI SUWANDARI; IRMAYANTI TRI KURNIA; PREECHA PHUWAPRAISIRISAN; MUHAMMAD IQBAL HASAN; KIETTIPUM PHONTREE; JOHAN SETIAWAN; SALAMUN SALAMUN; NANANG DWI WAHONO; AGUS SUPRIYANTO; MOCH. AFFANDI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 10 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251042

Abstract

Abstract. Ramadhan R, Ni’matuzahroh, Lestari DA, Hariyanto NA, Suwandari S, Kurnia IT, Phuwapraisirisan PP, Hasan MI, Phontree K, Setiawan J, Salamun, Wahono ND, Supriyanto A, Affandi M. 2024. Bioprospecting of non-timber forest products of selected flora in Baluran National Park, East Java, Indonesia for phytochemicals, anti-diabetic, anti-tyrosinase, and antioxidants. Biodiversitas 25: 3803-3815. The ecosystem within Baluran National Park comprises elements that exhibit varying degrees of sensitivity to environmental influences, encompassing both biotic and abiotic factors. It has been observed that identical plant species may display significant variations in their secondary metabolite content across different habitats. The primary objective of this investigation is to analyze the phytochemical composition, antioxidant properties, anti-tyrosinase effects, and anti-diabetic potential of specific flora species found in Baluran National Park. Furthermore, the research aims to identify novel natural resources with anti-diabetic and antioxidant properties among the selected flora from Baluran National Park, East Java, using chemotaxonomic and ethnopharmacological data. The study involved the qualitative and quantitative analysis of phytochemicals, along with assessing their in vitro anti-diabetic, anti-tyrosinase, and antioxidant activities through various assays such as ?-glucosidase inhibition, tyrosinase inhibition, and free radical scavenging tests (DPPH, ABTS, CUPRAC, and FRAP). The findings reveal that ethanol extracts of Euphorbia atoto demonstrated potent ?-glucosidase inhibitory activity with a percent inhibition (PI) value of 92.07±1.43%, surpassing the inhibitory effects of acarbose (25.92±1.61%) and quercetin (84.79±1.18%). Additionally, the antioxidant assessments indicated that the selected flora from Baluran National Park exhibited substantial antioxidant properties, as evidenced by their percent inhibition (PI) values. These results provide a scientific basis for considering the flora of Baluran National Park as a potential source of natural medicinal compounds.
Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen) FATIMAH FATIMAH; LILLAH ASRITAFRIHA; SYARIFAH SALSABILA; ERNA KRISTIANA DEWI; NI’MATUZAHROH NI’MATUZAHROH; ALMANDO GERALDI; RICO RAMADHAN; HERY SUWITO; ARRIZAL RAHMAN; LUKMAN RIYADI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 9 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250922

Abstract

Abstract. Fatimah, Asritafriha L, Salsabila S, Dewi EK, Ni’matuzahroh, Geraldi A, Ramadhan R, Suwito H, Rahman A, Riyadi L. 2024. Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen). Biodiversitas 25: 3005-3013. Etlingera balikpapanensis A.D. Poulsen is a new species of the Zingiberaceae family, which was discovered as an endemic species in the Balikpapan, Kalimantan. This study aimed to isolate endophytic bacteria from endemic Balikpapan ginger (E. balikpapanensis) and evaluate its antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans and to identify the most promising antimicrobial isolates. Plant samples include roots, stems, and leaves. The process of isolating bacteria from various plant parts, such as roots, stems, and leaves, involved repeated subculturing until pure isolates were obtained. Seven pure isolates were successfully obtained, coded as 6D, 5R, 2R, 3D1, 10D, 4R2, and 2D. Macroscopic, microscopic, and biochemical characterization of the seven isolates was carried out. The seven endophytic bacteria had varied macroscopic characteristics and were Gram-positive. The disc diffusion method was used to determine the ability of isolates to produce antimicrobial agents. The isolates showed antibacterial activity against E. coli and S. aureus. Isolates 3D1 and 5R showed the highest antibacterial activity against E. coli (8.20±0.47 mm and 8.57±0.08 mm), and 5R also showed the highest antibacterial activity against S. aureus (8.14±0.49 mm). These results demonstrate the potential of these isolates as effective antimicrobial agents. The 16S rRNA gene analysis revealed that isolate 3D1 was identified as Bacillus cereus strain PJA4.2 and isolate 5R as Micrococcus luteus strain SGAir0127 with similarity of 99.55% and 100%, respectively.
Isolation and potential test of indigenous bacteria from Singolangu, Magetan, Indonesia for bioremediation of organophosphate compounds PUJIATI PUJIATI; YULIA WULAN PRASETYANTI; MUH. WASKITO ARDHI; FATIMAH FATIMAH; RICO RAMADHAN; NI'MATUZAHROH NI'MATUZAHROH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 3 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260301

Abstract

Abstract. Pujiati, Prasetyani YW, Ardhi MW, Fatimah, Ramadhan R, Ni’matuzahroh N. 2025. Isolation and potential test of indigenous bacteria from Singolangu, Magetan, Indonesia for bioremediation of organophosphate compounds. Biodiversitas 26: 1029-1038. The objective of this study was to isolate indigenous bacteria from soil contaminated with pesticide residues and assess their potential for bioremediation of organophosphate compounds, specifically from the profenofos and chlorpyrifos groups. The methodology employed was bacterial isolation, characterization, and ex-situ bioaugmentation testing with a design that included six treatments. The parameters observed included pH, N, P, K, profenofos, and chlorpyrifos pesticide residues. By the fourth week, the bioremediation results demonstrated the exceptional effectiveness of bacteria from the Pseudomonas genus, particularly isolate B3, which outperformed other isolates in terms of N, P, and K pH values, which were 90 ppm, 94 ppm, 175 ppm, and 6, respectively. The lowest N, P, K was isolate B1, with consecutive values of 47, 63, 129, and a pH value of 6. The control soil on pesticide residue levels in the chlorpyrifos test was 150 ppm, and profenofos was 29 ppm. In the results of soil testing with Pseudomonas bacteria (isolate B3), chlorpyrifos levels were reduced by 41 ppm to 109 ppm, and profenofos levels were reduced by 21.8 ppm to 7.2 ppm. Isolate B1 was able to reduce profenofos levels by 18 ppm to 132 ppm and decrease profenofos by 9.2 ppm to 18.8 ppm. The test results showed that the type of indigenous bacteria and the length of incubation time have an impact on soil quality and pesticide residue levels.
Co-Authors AGUS SUPRIYANTO ALIFAH NASTITI ALMANDO GERALDI ARRIZAL RAHMAN DELA DWI ALAWIYAH DINDA AYU LESTARI ERNA KRISTIANA DEWI FATIMAH FATIMAH HERTANTI HERTANTI HERY SUWITO IRMAYANTI TRI KURNIA JOHAN SETIAWAN JUNAIRIAH JUNAIRIAH KIETTIPUM PHONTREE LILLAH ASRITAFRIHA LUKMAN RIYADI MOCH. AFFANDI MUH. WASKITO ARDHI MUHAMMAD IQBAL HASAN NANANG DWI WAHONO NAZIL DWI RAHAYUNINGTYAS NI'MATUZAHROH NI'MATUZAHROH NI’MATUZAHROH NI’MATUZAHROH NOVA ALFIAN HARIYANTO PREECHA PHUWAPRAISIRISAN PUJIATI PUJIATI R. BEKTI KISWARDIANTA SALAMUN SALAMUN SUHARTINI SUWANDARI SYARIFAH SALSABILA YULIA WULAN PRASETYANTI