DNA Extraction of Cynopterus brachyotis of Labuhan Ratu VII Based on Column Method in Supporting its Molecular Species Confirmation

Annisa Lidya Maharani (Universitas Lampung, Bandar Lampung)
Elly Lestari Rustiati (Universitas Lampung, Bandar Lampung)
Priyambodo Priyambodo (Universitas Lampung, Bandar Lampung)
Eko Agus Srihanto (Lampung Disease Investigation Center)
Enny Saswiyanti (Lampung Disease Investigation Center)
Dian Neli Pratiwi (Institute Technology of Sumatra)
Alvin Wiwiet Susanto (Akar Lestari Indonesia, Bandar Lampung)

Journal Of Agriculture Sciences (Agrosci)
Vol 3 No 1 (2025): Vol 3 No 1 September 2025

Background. Bats (Chiroptera) are true flying mammals and nocturnal. Ecologically, bats play important roles as pollinators, seed dispersers, guano producers, biocontrol, and hosts a range of viruses. Cynopterus brachyotis belongs to fruit-eating bats and is considered morphologically cryptic with C. sphinx. Aims. To support morphological identification, molecular species confirmation needed to be conducted. DNA extraction, as an initial step, plays a crucial role in molecular analysis. Methods. The quality and quantity of extracted DNA determine the success rate of sequencing for species confirmation in bats. In this study, oropharyngeal samples were used. Result. The research procedures included bat capture and DNA oropharyngeal swab sample collection, sample preparation, DNA extraction using a silica column-based commercial kit, DNA concentration measurement using a Qubit assay, and electrophoresis. Conclusion. The Qubit assay showed DNA concentrations of >1 ng/µL, while electrophoresis did not reveal bright DNA bands.