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All Journal Biotropika Jurnal Pembelajaran dan Biologi Nukleus Jurnal Kolaboratif Sains
Marina Ratte
Teknologi Laboratorium Medis, Universitas Widya Nusantara
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Morphological Characterization and Antioxidant Activity of Actinobacteria from Xylocarpus granatum Growing in Mangrove Habitat Ratte, Marina; Batubara, Irmanida; Lestari, Yulin
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.01

Abstract

Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. Granatum and determine its antioxidant activity. The research method begins with the isolation of endophytic actinobacteria from fruits, seeds, and leaves using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaf. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. DNA extraction and 16S rRNA amplification from endophytic actinobacteria X. granatum according to Presto Mini gDNA Bacteria Kit Protocol. Based on 16S rDNA gene analysis, isolates XGF12 and XGF11 showed 99.16% and 99.42% similarities with Streptomyces xylanilyticus, respectively. Meanwhile, XGF4 showed to have 99.82% similarities with Brevibacterium sediminis.
Quantification of Extracellular α-Amylase Activity from Bacillus subtilis in a Strach Supplemented Medium Using Reducing Sugar Analysis Yuyun Nisaul Khairillah; Marina Ratte
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i2.9452

Abstract

Background: α-Amylase is an extracellular enzyme responsible for the degradation of starch by cleaving α-1,4-glycosidic linkages, thereby producing shorter reducing sugars. This reaction holds significance due to the abundance, cost-effectiveness, renewability, and widespread use of starch as a raw material in numerous bioprocesses. In the context of industrial starch processing, the hydrolysis mediated by amylase is integral to the liquefaction and saccharification stages, which are critical in determining the efficiency of syrup production, brewing, baking, bioethanol production, and other processes reliant on starch. Methodology: This study aims to evaluate the extracellular α-amylase activity from Bacillus subtilis cultivated in nutrient broth supplemented with 1% (w/v) soluble starch. The culture was incubated at 37°C for 24 h under shaking conditions at 130 rpm and an initial medium pH of 7.0. The crude extracellular enzyme was obtained by centrifugation at 6,000 rpm for 20 min. α-Amylase activity was determined using the 3,5-dinitrosalicylic acid (DNS) assay by measuring reducing sugars released from soluble starch hydrolysis, with maltose as the calibration standard. The enzyme activity was determined in U/mL by spectrophotometric analysis at 550 nm. Findings: The maltose standard curve produced the regression equation y = 0.7849x + 0.0938 and an R² value of 0.9732. The B. subtilis culture grown in starch-supplemented nutrient broth showed a mean absorbance of 2.3915 and an α-amylase activity of 0.116 U/mL. These results showed that                         B. subtilis secreted detectable extracellular α-amylase under the tested conditions, although the activity was low. Contribution: The addition of starch contributed to enzyme induction because starch functions as both a substrate-related carbon source and a signal for amylolytic enzyme production. Therefore, nutrient broth supplemented with 1% starch can be used as a simple medium for preliminary extracellular α-amylase production by B. subtilis
Profil Mikrobiologis Infeksi Kulit: Isolasi dan Karakterisasi Bakteri dari Infeksi Kulit: Microbiological Profile of Skin Infection:Isolation and Characterization of Bacteria from Skin Infections Marina Ratte; Andi Wirdani Pettalolo; Ririn Elpira
Jurnal Kolaboratif Sains (Special Issue) - Jurnal Kolaboratif Sains (JKS) - Januari 2026
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v9i1.10355

Abstract

Infeksi kulit merupakan masalah kesehatan yang masih sering ditemukan dalam praktik klinis dan umumnya berkaitan dengan infeksi bakteri, baik yang bersifat komensal maupun patogen. Penelitian ini bertujuan untuk mengetahui profil mikrobiologis bakteri penyebab infeksi kulit melalui metode isolasi dan karakterisasi bakteri. Penelitian ini bersifat deskriptif dan menggunakan pendekatan laboratorium yang dilaksanakan selama tiga bulan di Laboratorium Mikrobiologi Program Studi D4 Teknologi Laboratorium Medis Universitas Widya Nusantara. Sampel penelitian berasal berupa swab bisul, infeksi luka, dan alergi. Isolasi bakteri dilakukan menggunakan media Mannitol Salt Agar (MSA), MacConkey Agar (MCA), dan Blood Agar Plate (BAP) dengan waktu inkubasi selama 3 x 24 jam, dilanjutkan dengan pewarnaan Gram dan pengujian katalase. Hasil penelitian menunjukkan bahwa pertumbuhan bakteri paling optimal diperoleh pada media MSA dan BAP. Jumlah koloni terbanyak diperoleh dari sampel swab infeksi luka, yaitu sebanyak 58 koloni sedangkan pada swab bisul diperoleh 21 koloni pada media MSA. sebanyak 9 isolat terpilih menunjukkan karakteristik dominan sebagai bakteri Gram positif berbentuk kokus yang tersusun berkelompok menyerupai buah anggur serta memberikan hasil uji katalase positif. Meskipun demikian, beberapa isolat bakteri juga menunjukkan karakteristik Gram negatif. Hasil penelitian ini menunjukkan bahwa bakteri Gram positif berperan dominan sebagai penyebab infeksi kulit pada sampel yang diteliti dengan adanya keterlibatan bakteri lain pada kondisi tertentu.
Karakteristik Bakteri Telapak Tangan Melalui Pewarnaan Gram dengan Perlakuan Cuci Tangan dan Tanpa Cuci Tangan: Characteristics of Palm Bacteria Through Gram Staining with and Without Hand Washing Treatment Andi Wirdani Pettalolo; Marina Ratte; Arsella Indra Lestari
Jurnal Kolaboratif Sains (Special Issue) - Jurnal Kolaboratif Sains (JKS) - September 2025
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v8i9.8680

Abstract

Telapak tangan merupakan salah satu media penting dalam penyebaran mikroorganisme patogen. Penelitian ini bertujuan untuk mengidentifikasi karakteristik bakteri pada telapak tangan melalui perbandingan hasil pewarnaan Gram antara sampel tanpa cuci tangan dan setelah cuci tangan. Penelitian dilaksanakan secara deskriptif menggunakan metode pewarnaan Gram terhadap isolat bakteri yang diperoleh dari usapan telapak tangan. Hasil penelitian menunjukkan bahwa dari delapan isolat bakteri, tujuh di antaranya bersifat Gram positif dan satu isolat bersifat Gram negatif. Pada sampel tanpa cuci tangan lebih dominan ditemukan bakteri Gram positif dengan morfologi basil dan kokus, sedangkan pada sampel setelah cuci tangan jumlah bakteri lebih sedikit namun masih ditemukan bakteri Gram positif dengan bentuk basil dan kokus. Penelitian ini menyimpulkan bahwa kebiasaan mencuci tangan berperan dalam menurunkan jumlah bakteri pada telapak tangan, meskipun tidak sepenuhnya menghilangkan keberadaan bakteri.
Co-Authors Arsella Indra Lestari Elpira, Ririn Irmanida Batubara Pettalolo, Andi Wirdani YULIN LESTARI Yuyun Nisaul Khairillah