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All Journal Journal of the Indonesian Tropical Animal Agriculture Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Tropical Animal Science Journal
Budiman, C.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Veterinary

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The growth and production of antimicrobial compounds from Lactobacillus plantarum IIA-1A5 on cheese whey medium Mutmainna, A.; Arief, I. I.; Budiman, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.173-184

Abstract

Plantaricin IIA-1A5 is a bacteriocin produced by Lactobacillus plantarum IIA-1A5 which is isolated from Indonesian beef, and it inhibits activity of Gram negative and positive pathogenic bacteria. However, preparation of the antibacterial agent for further applications or studies is costly due to the usage of a bacterial medium. Therefore, this study was aimed to investigate the feasibility of cheese whey as a growth medium for production of the bacteriocin. The growth curve of Lactobacillus plantarum IIA-1A5 in producing antimicrobial compounds was found to occur in the logarithmic phase with an incubation time of 28 and 32 hours. Purification of plantaricin IIA-1A5 produced peptides with a molecular weight of 9.59 kDa consisting of whey and whey+ (20g/L sucrose, 12.5 g/L tryptone and 7.5 g/L yeast extract); thus, the peptide was grouped as class IIa (<10 kDa) bacteriocin. The protein concentration of plantaricin IIA-1A5 with whey+ (sucrose, tryptone, and yeast extract) treatment and whey treatment was 1883.17 mg/ml and lower than 325.58 mg/ml, respectively. Based on the antimicrobial activity test using a paper disc method, plantaricin IIA-1A5 demonstrated antimicrobial activity against Staphylococcus aureus ATCC 25923; the whey and whey+ treatment yielded 38.02 IU/dL and 321 IU/dL, respectively, while antimicrobial activity against Escherichia coli ATCC 25922 using whey and whey+ treatment yielded 44.85 IU/dL and 172.08 IU/dL, respectively. The effectiveness of the antimicrobial properties of plantaricin IIA-1A5 in the whey medium is proven through the results of this study. In short, the whey is appropriate growth medium for bacteriocin production.
Profiling of Dominant Bacteria in Traditional Buffalo Milk Cheese “Dangke” Based on 16S rRNA Sequencing Mutmainna, A.; Arief, I. I.; Budiman, C.; Abidin, Z.; Wulandari, Z.
Tropical Animal Science Journal Vol. 48 No. 5 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.5.450

Abstract

Metagenomic approaches are highly valuable in food microbiology, particularly for the investigation of traditional fermented products such as dangke buffalo milk cheese from South Sulawesi, Indonesia. Buffalo milk, a primary raw material, contains diverse biochemical and bioactive components that are produced by microbial activity during fermentation. Unlike conventional culture-based techniques, metagenomics enables the comprehensive characterization of microbial communities directly from food matrices. This study aimed to identify the key microbial taxa that contribute to the dangke quality of fermentation and to understand the factors influencing it. Bacterial 16S rRNA genes were amplified using primers 27F and 1492R under optimized polymerase chain reaction conditions. DNA concentrations were measured with NanoDrop and Qubit instruments, and sequencing was conducted using Oxford Nanopore Technology with MinKNOW software (v23.04.5). Metagenomic analysis revealed Enterococcus faecium as the dominant lactic acid bacterium across all dangke samples (P1–P3), emphasizing its crucial role in fermentation and probiotic potential. These findings suggest that E. faecium can enhance the quality and functional properties of dangke. Moreover, metagenomic tools can support the development of standardized fermentation practices and microbial safety assessments for traditional dairy products.
Produktivitas Entok Betina dengan Pemberian Pakan Terbatas Selama Periode Pertumbuhan Ayuningtyas, G.; Jakaria, Jakaria; Rukmiasih, Rukmiasih; Budiman, C.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 4 No. 2 (2016): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

The objective of this research was to study the effect of restricted feeding on muscovy duck performance’s in grower period, the development of reproduction organ, puberty characteristic, egg production, and blood lavel of cholesterol and trigliseride. Total 32 of female muscovy duck , 8-22 weeks age ware evaluated in this research. A completely randomized design with 3 level treatments were used to arrange the treatments. The treatments of restricted feeding were ad libitum consumption ( P1); 70% ad libitum (P2) and 40 % ad libitum (P3). The diet contain 22% crude protein (0-3 weeks age), 18% crude protein (4-7 weeks age), and 14% crude protein (8-22 weeks age). The parameters observed were: performance’s in grower period (feed consumption, body weight, total daily gain), puberty characteristic (puberty age, body weight on puberty, first egg weight), egg production on first cycle, the development of reproduction organ, and blood lavel of cholesterol and trigliseride. The results showed that body weight of muscovy duck P2 and P3 lower then P1. The restricted feeding could delayed development of folicle ovary and  age puberty. The blood level of cholesterol’s muscovy duck P2 and P3 lower then moscovy duck P1.
Optimasi Ekstraksi DNA Genomik Probiotik Lactobacillus plantarum IIA-1A5 dari Daging Sapi Peranakan Ongole untuk Sekuensing Genom Utuh Budiman, C.; Arief, I. I.; Yusuf, M.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 6 No. 1 (2018): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

Lactobacillus plantarum IIA-1A5 is an Indonesian probiotic bacterium isolated from Ongole grade cattle. This strain has demonstrated series of benefical properties and promising to be further applied in development of functional foods. Attempts to further exploit this strain require comprehensive understanding on its molecular properties, in particular is the genes responsible for its probiotic properties.Whole genome sequencing (WGS) is currently an affordable tool to fully decipher genetic organization of the bacteria, yet often limited by the requirement of high amount of high quality of genomic DNA ofsample. This study aims to develop an optimum method for obtaining high quality genomic DNA of L. plantarum IIA-1A5 in sufficient amount of WGS study. To address, genomic DNA of L. plantarum IIA-1A5 was extracted from growth phase of adaptation, exponential and stationary. The result showed that exponential and stationary phases were able to yield genomic DNA higher than minimum requirement for WGS. Nevertheless, the quality of genomic DNA from both phases were considerably low, according to their 260/280 and 260/230 ratio. The worst quality was found on 260/280 ratio referring to thepresence of high amount of proteinaceous compounds in the sample. Further optimization was done on genomic DNA from stationary phase by adding proteinase K at various incubation time. It showed that the incubation for 7.5 to 10 hours yielded acceptable purity and amount of genomic DNA for WGS. In addition, we also observed a trade-off phenomenon of yield-purity genomic DNA of L. plantarum IIA-1A5. Altogether, the optimized method here should contribute to further molecular studies of this probiotic strain.
Co-Authors Arief, I. I. G. Ayuningtyas Jakaria Jakaria M. Yusuf Mutmainna, A. Mutmainna, Andi Rukmiasih Rukmiasih Wulandari, Z. Z. Abidin