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Dang Meka, Aderia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Evaluation of the Antioxidant Activity of Jatropha curcas L. Leaf Extract Gel Formulation using the DPPH Method Dang Meka, Aderia; Putri Vidiasari Darsono; Darini Kurniawati; Rohama
BIOEDUSCIENCE Vol 10 No 2 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20795

Abstract

Background: Free radical exposure poses significant threats to public health by damaging cells and causing degenerative diseases like cancer and premature aging. Antioxidants are vital in neutralizing these radicals. Jatropha curcas L. leaves contain bioactive compounds with promising antioxidant and wound healing properties, warranting evaluation in a practical gel form. Methods: This study used a true experimental design with a posttest-only control group; quercetin was the control, while the treatment groups received different gel formulations of Jatropha curcas L. leaf extract. Results: The pure extract had very strong antioxidant activity (IC50 0.32 ppm). Gel formulations (Formula 2 and Formula 3) also exhibited strong antioxidant activity, with IC50 values of 30.90 ppm and 35.85 ppm, respectively; Formula 2 performed better. Quercetin showed strong activity at 1.22 ppm. One-way ANOVA (p < 0.01) confirmed significant differences between groups. Conclusions: The Jatropha curcas L. leaf extract gel has significant antioxidant potential and is effective in preventing oxidative damage, supporting its use in safe, gel-based herbal skin products.
Co-Authors Darini Kurniawati Putri Vidiasari Darsono, Putri Vidiasari Rohama