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All Journal Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Abdimas Dewantara MITRA: Jurnal Pemberdayaan Masyarakat Jurnal Pengabdian UNDIKMA Biodiversitas Journal of Biological Diversity
Tan Watumesa Agustina
Faculty Of Biotechnology, Universitas Katolik Indonesia Atma Jaya, Kampus BSD, Jalan Raya Cisauk-Lapan No. 10, BSD City, Tangerang 15345, Banten, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Analisis Matagenom Komunitas Bakteri Tempe dengan Teknik Terminal Restriction Fragment Length Polymorphism (T-RFLP) Barus, Tati; Griselda, Griselda; Suwanto, Antonius; Agustina, Tan Watumesa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.198 KB) | DOI: 10.24002/biota.v15i2.2726

Abstract

Bacteria have an important role in tempe fermentation in Indonesia, aside of Rhizopus oligosporus as the dominant microbe. In this study the molecular aspect of bacterial diversity in tempe were analyzed using a fingerprinting technique, Terminal-Restriction Fragment Length Polymorphism (T-RFLP). This study was aimed to examine the diversity of bacterial community during tempe making. Bacterial diversity analysis was conducted in the first hour and the thirteenth hour after the soybean soaked while the fresh tempe was analysed at one to two hours after the fermentation ended. T-RFLP can be used to describe the diversity of bacterial community during the fermentation of tempe. T-RFLP profiles revealed the presence of 24, 30 and 33 bacterial phylotypes in the first hour and the thirteenth hour after the soybean soaked as well as in fresh tempe samples. The phylotypes were dominated by unculturable bacteria group. Only several bacterial phylotypes were consistenly identified since the beginning to the end of fermentation, while most of them were only identified at certain phases along with the environmental changes (i.e: pH) that occured during the fermentation process. One of the consistently identified groups belongs to Bacillus genera.
Enrichment of Anti Hoax Competency among Secondary School Students Watumesa Agustina Tan; Theodorus Eko Pramudito
MITRA: Jurnal Pemberdayaan Masyarakat Vol. 6 No. 2 (2022): Mitra: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v6i2.2555

Abstract

Hoax and misinformation are common occurrences in the current era of social media. Nurturing critical thinking is necessary to combat the proliferation of hoaxes, especially in the younger generation. In this activity, we conducted a workshop for high school students and teachers to build awareness of hoaxes and how to identify them. This in-person workshop was held at Canisius College High School in a three-hour session. After taking part in the workshop, participants were expected to be able to explain the definition of a hoax, explain why hoaxes exist and are spread out among the community, and analyze the credibility of new information. For this purpose, the workshop consisted of three sessions. In the first session, the facilitators walked all participants through a lively discussion on what hoaxes are and how they get passed on from one person to another. In the second session, we introduced the concept of credibility to the participants. In the final session, the participants practiced evaluating the credibility of news. All sessions were delivered as paired or group discussions using authentic articles or postings from various social media platforms. The evaluation survey at the end of the activity showed that the participants agreed that the topic covered by this workshop was necessary and useful; however, they felt that the event was too short. This opportunity provided insights into how to improve similar hoax awareness-raising programs in the future.
Bacterial Community Profiles in Tapai Singkong: a Traditional IndonesianFermented Food from Cassava Tubers Tati Barus; Andiny Ndu Ufi; Watumesa Agustina Tan; Ana Lucia Ekowati; Adi Yulandi
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.225 KB) | DOI: 10.5454/mi.16.2.1-7

Abstract

Tapai singkong is one of the popular fermented foods in Indonesia, which is processed from cassava tubers (Manihot utilissima ). The bacteria present during the fermentation process de Manihot utilissima determines the quality of Tapaisingkong. However, information about the bacteria of Tapai singkong is still limited.  Therefore, this study aimed to analyze the bacterial community of based on culturing techniques Tapai singkong and based on metagenomic sequencing with Next-Generation Sequencing (NGS) techniques. Five types of samples were Tapai singkong obtained from producers in Jakarta, Bogor, Tangerang, Band Tapai singkong ung, and Kediri-Indonesia. The bacterial community in this study was studied in from Kediri Tapai singkong because the taste was most favored by the panelists based on the hedonic test. Based on the culture technique using De Man Rogosa and Sharp Agar media, the two most abundant bacterial isolates were found. Based on the 16S rRNA gene sequence, both isolates were the same lactic acid bacteria (LAB), namely Pediococcus acidilactici DSM 20284, with 99.6% similarity. Based on metagenomic sequencing, it was found that the bacteria in the consisted of Firmicutes Tapai singkong (82%), Bacteriodetes (10%), unidentified bacteria (5%), and Verrucomicrobia (1%). The genus of Firmicutes was dominated by the LAB group, namely Pediococcus (61.23%), Weissella (4.8%), Lactobacillus (3.9%), Sporolactobacillus (2.2%), and Staphyloccocus (2.1%). The results of this study showed that the LAB group was most abundant in Tapai singkong . Therefore, the role of each LAB needs to be studied further to determine its role in the quality of Tapai singkong.
Sosialisasi implikasi minyak jelantah kepada pedagang kantin di Kampus BSD, Universitas Katolik Indonesia Atma Jaya Daru Seto Bagus Anugrah; Adeline Mayvie Wijanarko; Juan David Sinanu; Yanti Yanti; Watumesa Agustina Tan; Krismanto Rabin Chandra
ABDIMAS DEWANTARA Vol 6 No 2 (2023): Article in Press
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/ad.v6i2.15731

Abstract

Minyak jelantah adalah minyak bekas yang diperoleh dari hasil menggoreng makanan. Penggunaan minyak jelantah berulang menyebabkan penurunan kualitas lingkungan dan manusia. Minyak jelantah mengandung zat polifenol dan akrilamida yang meningkatkan risiko kanker dan penyakit lainnya. Selain itu, minyak jelantah juga mempengaruhi kualitas makanan, menyebabkan perubahan rasa dan tekstur makanan. Pembuangan minyak jelantah sembarangan menyebabkan penumpukan pada saluran air dan tempat pembuangan sampah, berdampak negatif pada ekosistem dan organisme di perairan dan tanah. Namun demikian, minyak jelantah masih dapat dimanfaatkan menjadi produk yang lebih bermanfaat. Melihat hal ini, Universitas Katolik Indonesia Atma Jaya (Unika Atma Jaya) Bersama dengan PT Ubah Limbah Indonesia bekerja sama untuk mengedukasi pedagang di kantin Atma Jaya sebagai salah satu bagian masyarakat yang sering menggunakan minyak goreng. Tujuan kegiatan ini adalah untuk meningkatkan pengetahuan pedagang mengenai minyak jelantah serta bagaimana cara mengolahnya menjadi produk lain seperti lilin aromatik. Penyampaian materi dari empat pembicara menunjukkan adanya peningkatan pengetahuan dari pedagang mengenai minyak jelantah, dilihat dari hasil pre-test dan post-test serta analisis Wilcoxon Rank Test. Secara keseluruhan, upaya kolaboratif antara Unika Atma Jaya dan PT Ubah Limbah Indonesia untuk memberikan edukasi kepada pedagang kantin telah berhasil meningkatkan pengetahuan pedagang mengenai potensi dan pengolahan minyak jelantah.   Socialisation of the implications of used cooking oil to the canteen vendors at the BSD Campus of Universitas Katolik Indonesia Atma Jaya   Abstract: Used cooking oil is the residual oil obtained from frying food. Repeated use of used cooking oil leads to a decline in environmental and human quality. Used cooking oil contains polyphenols and acrylamide compounds that elevate cancer risk and other diseases. Furthermore, used cooking oil affects the quality of food, inducing alterations in taste and texture. Improper disposal of used cooking oil results in accumulation within waterways and waste disposal sites, adversely impacting ecosystems and aquatic and terrestrial organisms. Nevertheless, there is potential to repurpose used cooking oil into more beneficial products. Recognizing this, Atma Jaya Catholic University of Indonesia (Unika Atma Jaya), in collaboration with PT Ubah Limbah Indonesia, endeavors to educate vendors at the Atma Jaya canteen, a segment of society that frequently employs cooking oil. This initiative aims to enhance vendors' understanding of used cooking oil and its transformation into alternative products such as scented candles. Delivery of content by four presenters demonstrates an improvement in vendors' comprehension of used cooking oil, evident from pre-test and post-test results and Wilcoxon Rank Test analysis. Overall, the collaborative effort between Unika Atma Jaya and PT Ubah Limbah Indonesia to educate canteen vendors has effectively elevated their knowledge regarding the potential and processing of used cooking oil.
Lokakarya Berbasis Eksperimen sebagai Upaya Peningkatan Literasi Sains Siswa Sekolah Dasar: Pengenalan Konsep Indikator pH Alami Melalui Tinta Tak Terlihat Watumesa Agustina Tan; James Elliot Christanto; Ronald Kristianto Wibowo; Listya Utami Karmawan
Jurnal Pengabdian UNDIKMA Vol. 7 No. 1 (2026): February
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v7i1.17506

Abstract

This community service program aims to enhance elementary school students’ understanding of and interest in science through the implementation of interactive experiments based on the concept of natural pH indicators. The program was conducted at SD Athalia, Tangerang, involving 132 fifth-grade students who were divided into five groups of 26–27 students to ensure active participation. The implementation stages included an introduction to basic pH concepts, experimental demonstrations, and hands-on activities in which students created drawings using “invisible ink” made from a baking soda solution as a basic substance and turmeric as a natural pH indicator. The turmeric solution changed color from yellow to reddish-brown in areas that reacted with the base, revealing previously hidden images. The results indicate high levels of student enthusiasm, active participation, and curiosity toward science learning. These findings demonstrate that simple, activity-based experimental approaches are effective in fostering students’ interest in and initial understanding of science, while also representing a tangible contribution of higher education institutions to improving community science literacy.
Ramularia mali strains isolated from petroleum product-contaminated soil are capable to grow on multiple aromatic compounds Watumesa Agustina Tan; Helen Surya Teja; Stephanie STEPHANIE
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210823

Abstract

Abstract. Tan WA, Teja HS, Stephane. 2020. Ramularia mali strains isolated from petroleum product-contaminated soil are capable to grow on multiple aromatic compounds. Biodiversitas 21: 3590-3595.  Aromatic compounds are present as a complex mixture in the environment, some of which are toxic and carcinogenic in humans and animals. In this study, we reported the first instance of arthroconidial yeast growth on more than one aromatic compound. Yeasts were isolated from soil surrounding gas stations and screened for growth on phenol, followed by qualitative growth testing on chlorobenzene, aniline, toluene, 2-nitrotoluene, benzoate, and naphthalene. Pure isolates identity and phylogeny were determined based on the ITS region. All isolates grew on phenol and benzoate, but none grew on naphthalene. Aside from the inability to grow on naphthalene, SR8 grew on other tested aromatics, while SR3 showed similar growth ability except on 2-nitrotoluene. Growth on 2-nitrotoluene was observed for PPS3, PB4, SR1, SR6, and SR8, a phenotype that has yet been reported in yeasts thus far. All isolates shared 99.81-100% similarity and were phylogenetically clustered with the arthroconidial yeast Ramularia mali CBS 129581, despite that some of them were morphologically different and had varying capability to grow on tested aromatics. Therefore, ITS sequences cannot be used to differentiate Ramularia mali up to the strain level. Overall, we demonstrated the diverse potential of yeast strains in reducing aromatic compound contamination.
Genetic diversity of phenol hydroxylase-encoding genes among wastewater sludge bacteria WATUMESA AGUSTINA TAN; FEDHO KUSUMA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221021

Abstract

Abstract. Tan WA, Kusuma F. 2021. Genetic diversity of phenol hydroxylase-encoding genes among wastewater sludge bacteria. Biodiversitas 22: 4291-4297. Phenol is a toxic aromatic compound that is used in various industries. In this study, we explore the molecular diversity of bacterial genes that encode for phenol hydroxylase, a key enzyme for phenol degradation, within sludge samples collected from the aeration and microbial recovery tank of an automotive wastewater treatment system. Partial phenol hydroxylase-encoding gene (600 bp) was amplified from the total DNA of each tank using a degenerate primer pair and cloned to construct a gene library. A total of 37 and 38 clones were obtained from the aeration and microbial recovery tank, respectively. The clones were sequenced and compared to GenBank database using BLASTX, followed by neighbor joining-based phylogenetic analysis using MEGA7. A majority of the clones recovered from both tanks belonged to Betaproteobacteria, which have been reported as phenol degraders with various metabolic activities and lifestyles. The clones are grouped into eight clusters. Six clusters were present in both the aeration and microbial recovery tank, and one unique cluster was identified in each tank. This indicated that diverse microbial communities play a role in phenol-containing wastewater treatment. They may have changed throughout the process as the surrounding chemical compositions differed and a variety of metabolic roles surfaced.
Diversity of bacterial phenol hydroxylase-encoding genes from gasoline-contaminated silt soil SURAJ RAJAN VASANDANI; WATUMESA AGUSTINA TAN
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231062

Abstract

Abstract. Vasandani SR, Tan WA. 2022. Diversity of bacterial phenol hydroxylase-encoding genes from gasoline-contaminated silt soil. Biodiversitas 23: 5502-5506. Phenol is an aromatic compound often used as a raw material or intermediate in various industries. Improper handling and disposal may lead to the accumulation of this hazardous compound. Bioremediation is the most viable method to remove phenol from contaminated environment. In this study, the diversity of genes that encode for phenol hydroxylase, a key enzyme in phenol degradation, was assessed in gasoline-contaminated soil from a commercial gas station in Central Jakarta, Indonesia. Partial phenol hydroxylase-encoding gene library was constructed using a pair of universal primer in the pGEM®-Teasy vector. A total of 30 recombinant clones were obtained and sequenced to analyze the genetic diversity of this gene. Obtained clones were 86-99% identical to phenol hydroxylase-related proteins. Phylogenetic tree analysis on amino acid sequences derived from our library revealed that 56.7% of the cloned fragments were closely related to Proteobacteriodota and 20.0% of them were clustered with Actinomycetota. The rest 23.3% of the clones formed a cluster separate from any of the reference sequences, possibly indicating the presence of novel phenol hydroxylase genes.
Co-Authors Adeline Mayvie Wijanarko ADI YULANDI Ana Lucia Ekowati Andiny Ndu Ufi Antonius Suwanto Anugrah, Daru Seto Bagus FEDHO KUSUMA Griselda, Griselda Helen Surya Teja James Elliot Christanto Juan David Sinanu Krismanto Rabin Chandra Listya Utami Karmawan Ronald Kristianto Wibowo Stephanie STEPHANIE SURAJ RAJAN VASANDANI Tati Barus Theodorus Eko Pramudito Yanti, Yanti