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Isolation of MA-ACS Gene Family and Expression Study of MA-ACS1 Gene in Musa acuminata Cultivar Pisang Ambon Lumut LISTYA UTAMI KARMAWAN; SONY SUHANDONO; FENNY MARTHA DWIVANY
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.641 KB) | DOI: 10.4308/hjb.16.1.35

Abstract

Musa acuminata cultivar pisang ambon lumut is a native climacteric fruit from Indonesia. Climacteric fruit ripening process is triggered by the gaseous plant hormone ethylene. The rate limiting enzyme involved in ethylene biosynthesis is ACC synthase (ACS) which is encoded by ACS gene family. The objective of this study is to identify MA-ACS gene family in M. acuminata cultivar pisang ambon lumut and to study the MA-ACS1 gene expression. The result showed that there were nine M. acuminata ACS gene family members called MA-ACS1–9. Two of them (MA-ACS1 and MA-ACS2) were assessed using reverse transcriptase PCR (RT-PCR) for gene expression study and it was only MA-ACS1 correlated with fruit ripening. The MA-ACS1 gene fragment has been successfully isolated and characterized and it has three introns, four exons, and one stop codon. It also shows highest homology with MACS1 gene from M. acuminata cultivar Hsian Jien Chiao (GenBank accession number AF056164). Expression analysis of MA-ACS1 using quantitative PCR (qPCR) showed that MA-ACS1 gene expression increased significantly in the third day, reached maximum at the fifth day, and then decreased in the seventh day after harvesting. The qPCR expression analysis result correlated with the result of physical analysis during fruit ripening. Key words: pisang ambon lumut, MA-ACS gene family, gene characterization, gene expression, quantitative PCR (qPCR)
Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4) Listya Utami Karmawan; Fenny Martha Dwivany; Rizkita Rachmi Esyanti; I Nyoman Pugeg Aryantha
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.193 KB) | DOI: 10.4308/hjb.26.4.196

Abstract

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.
Towards Green Rusunawa: Clean Water Utilization, Composting, and Urban Farming in Muara Baru Flats Dion Notario; Ferdian Suprata; Meda Canti; Listya Utami Karmawan
MITRA: Jurnal Pemberdayaan Masyarakat Vol 5 No 1 (2021): MITRA: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v5i1.1077

Abstract

Waste disposal and clean water management are some of the common social issues arising in densely populated settlements, including Muara Baru flats, Jakarta. Some programs have been carried out to overcome these two issues in Muara Baru, such as the construction of a Rain Water Harvesting (RWH) facility and the provision of Trash Banks. Nevertheless, adequate support for the residents is still needed to boost their awareness of proper clean water management and waste disposal and to encourage the formation of urban agriculture, which is expected to elevate their living standards. Therefore, a community empowerment program in Muara Baru was developed, consisting of three sections: education on clean water management, compost making training, and urban farming. The success of this program was then measured using questionnaires and focus group discussions. A majority of residents responded positively to the program and were willing to actively participate by preparing the land, making compost, planting seeds, or caring for the harvest. There were indeed some obstacles hampering the program's success, such as lack of commitment from the local community, climate change, water availability, and arable land availability. To overcome these barriers and to promote the program’s sustainability, some suggestions are to be proposed, including proper selections of plant types and provision of support and motivation for the local community.
PENYEDIAAN SARANA CUCI TANGAN SEDERHANA UNTUK PENCEGAHAN PENYEBARAN COVID-19 DI PASAR GENTENG, SURADITA, CISAUK Listya Utami Karmawan; Widya Agustinah; Renna Eliana Warjoto; Daru Seto Bagus Anugrah; Danniella Yudianti; Illene Nanine; Eunice Catherine Witness
Jurnal Bakti Masyarakat Indonesia Vol 4, No 1 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i1.9487

Abstract

COVID-19, a source of distress in most countries, has encouraged world leaders to issue necessary policies to resolve this global pandemic. An alarming rate of infections in the community has pushed the Indonesian government to come up with the Large-Scale Social Restriction (Pembatasan Sosial Berskala Besar/PSBB). The faculty of Biotechnology of Atma Jaya Catholic University of Indonesia has since initiated a community service to halt the spread of COVID-19 by supplying simple hand washing facilities around Pasar Genteng Traditional market in Suradita, Cisauk. Prior to the installations, the traditional market lacked proper sanitizing facilities. Clean water sources such as taps are commonly situated far away from the marketplace. Traders and visitors crowd these areas each day, exponentially increasing the risk of spreading COVID-19. The crowded populations of the traditional market are a motivating factor for this community service, which aims to increase the public awareness in the prevention of COVID-19. Activities of this community service includes installations of hand washing facilities along with educational infographics of proper sanitizing procedures. A total of 21 participants attended the community service while maintaining physical distancing conditions and were encouraged to use the hand wash facilities at the end of the service. The community service was successful, providing impactful benefits towards the traders and visitors, with a raised community awareness of the importance of personal hygiene. The facilities are well maintained after 4 weeks and exemplary community awareness is displayed from their initiative that showed from the monitoring and evaluation results.  ABSTRAK:COVID-19, yang merupakan sumber penyebab keresahan berbagai negara di dunia telah menyebabkan pemerintah dunia mengeluarkan kebijakan untuk mengatasi pandemi yang terjadi secara global ini. Data di Indonesia menunjukkan angka terinfeksi yang terus meningkat sehingga pemerintah mengeluarkan kebijakan berupa Pembatasan Sosial Berskala Besar (PSBB). Oleh karena itu, Fakultas Teknobiologi Universitas Katolik Indonesia Atma Jaya berinisiatif melakukan kegiatan pengabdian masyarakat untuk menghambat tingkat penyebaran COVID-19 melalui penyediaan sarana cuci tangan sederhana di Pasar Genteng. Pasar tradisional ini terletak di kompleks Suradita, Cisauk. Sebelum kegiatan pemasangan, pasar ini kekurangan fasilitas sanitasi yang layak. Sumber air bersih seperti air sumur terletak jauh dari pasar dan belum ada akses air ledeng. Setiap hari pedagang dan pengunjung memadati pasar sehingga meningkatkan risiko penyebaran COVID-19. Oleh karena itu, kegiatan pengabdian masyarakat ini difokuskan pada pembuatan dan pemasangan fasilitas cuci tangan sederhana yang dilengkapi dengan infografis tentang cara mencuci tangan yang benar serta pembagian masker kain. Serah terima perangkat cuci tangan dan acara pembukaan dihadiri oleh 21 orang peserta dengan tetap memperhatikan protokol kesehatan. Kegiatan pengabdian masyarakat melalui penyediaan sarana cuci tangan sederhana di Pasar Genteng, Cisauk telah berlangsung dengan baik dan dirasakan manfaatnya oleh para pedagang dan pengunjung pasar. Sarana cuci tangan sederhana ini diharapkan dapat meningkatkan kesadaran masyarakat tentang pentingnya menjaga kebersihan diri dalam rangka pencegahan penyebaran COVID-19. Sarana cuci tangan tersebut masih dalam kondisi baik setelah empat minggu dan kesadaran masyarakat juga cukup baik yang terlihat dari hasil monitoring dan evaluasi.
Pelatihan pembuatan kombucha-ekstrak bunga telang untuk masyarakat di Desa Pagedangan, Tangerang, Banten Daru Seto Bagus Anugrah; Listya Utami Karmawan; Renna Eliana Warjoto; Widya Agustinah; William Frans; Jovita Aurelia; Maria Marcella; Christian Oetomo; Jesslyn Regina Alviana; Sentia Sentia; Theresia Ferenda
ABDIMAS DEWANTARA Vol 5 No 2 (2022)
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/ad.v5i2.12822

Abstract

Kombucha is a health drink made from fermented tea with Symbiotic Culture of Bacteria and Yeast (SCOBY), which can help consumers to maintain their immune systems. Due to the health benefits of Kombucha products, the Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, and the National Research and Innovation Agency (BRIN) conducted community service activities by training in making kombucha combined with butterfly tea extract. The purpose of this activity is to train the manufacture of kombucha food product-butterfly tea extract and explain the business potential of the kombucha product-butterfly tea extract to the residents of Pagedangan Village. To measure the increase in residents' knowledge, the service team conducted pre-test and post-test sessions using the quizizz application on each resident's cellphone. The results of the kombucha training in the village of Padegangan have had a positive and influential impact on increasing the knowledge of the residents who were tested using the Wilcoxon Rank Test analysis of the results of the pre-test and post-test. The material delivered to the four speakers showed positive results, which were judged by the results of filling out the event feedback link via platform forms. Overall, the training held was considered to have run smoothly.   Abstrak: Kombucha merupakan minuman kesehatan hasil fermentasi teh dengan Symbiotic Culture of Bacteria and Yeast (SCOBY) yang dapat membantu konsumennya untuk menjaga sistem imun. Dikarenakan manfaat kesehatan dari produk Kombucha, Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya dan Badan Riset dan Inovasi Nasional (BRIN) melakukan kegiatan pengabdian masyarakat dengan pelatihan pembuatan kombucha yang dipadukan dengan ekstrak bunga telang. Tujuan kegiatan ini adalah untuk melatih pembuatan produk pangan kombucha-ekstrak bunga telang dan memaparkan potensi usaha dari produk kombucha-ekstrak bunga telang kepada warga Desa Pagedangan. Untuk mengukur peningkatan pengetahuan warga, tim pengabdi melakukan sesi pre-test dan post-test menggunakan aplikasi quizizz pada handphone masing-masing warga. Hasil pelatihan kombucha di desa Padegangan sudah berdampak positif dan berpengaruh pada peningkatan pengetahuan warga yang diuji dengan menggunakan analisis Wilcoxon Rank Test terhadap hasil pre-test dan post-test. Penyampaian materi yang dilakukan pada keempat pembicara menunjukkan hasil yang positif yang dinilai dengan hasil pengisian link feedback acara melalui platform forms. Secara keseluruhan pelatihan yang diadakan sudah dinilai berjalan dengan lancar.
BioTech Talk: A Virtual Social Campaign Activity to High School Students on a Healthy Lifestyle Widya Agustinah; Listya Utami Karmawan; Renna Eliana Warjoto; Daru Seto Bagus Anugrah Bagus; Daniel Imanuel Yaurentus; Christian Kenneth
MITRA: Jurnal Pemberdayaan Masyarakat Vol. 7 No. 1 (2023): Mitra: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v7i1.3329

Abstract

Prospective students were included in the 12 to 18 years old age category of people who are permitted to obtain Covid-19 vaccine starting on June 2021. Face-to-face learning has been implemented soon after most students received full dose of vaccination. The objective of this program was to provide social campaign activities to high school students, particularly those who have enrolled in the Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia as new students in 2022, so that they could understand the importance of maintaining healthy lifestyle and following the recommended health protocols, obtain the right fact about Covid-19 vaccine, and act smart to reduce masks waste in order to prevent the transmission and infection of Covid-19 virus. The campaign “Biotech Talk” was performed virtually and in an asynchronous mode using an official social media account of the faculty, such as Instagram and YouTube. It was held for 6 weeks, starting on October 1st to November 16th, 2021, in the form of infographics and mini talk show videos. The same video content in Instagram received higher numbers of viewers and likes as compared to that in YouTube. Viewers were also encouraged to fill in the post-test and questionnaire of each episode as a method of evaluating the social engagement and interest of the video. The mean score of all episodes’ post-test was high (minimum 77 out of 100) and 60% of the respondents stated that the contents were clearly explained and relevant to the theme. Biotech Talk has been held in a timely manner and able to improve the viewer’s knowledge on healthy and smart lifestyle in the pandemic era as a preparation of face-to-face learning in the university.
Optimization of Composting Growing Media Time and Rice Husk Addition in Auricularia auricula Cultivation Dionysius Subali; Vasya Theodora Givianty; Anastasia Tatik Hartanti; Listya Utami Karmawan
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.30991

Abstract

 AbstractAuricularia auricula is a wood mushroom that is commonly consumed and is in demand by the people as a food ingredient because it contains many nutrients and health benefits. Cultivation of A. auricula was carried out using growing media and controlled environmental conditions. This study objective to determine the optimal combination of composting time and the rice husks addition in the A. auricula production. The treatment in this study was the composting time of the growing media for 0, 2, 4, 6, and 8 days, as well as the addition of rice husks as much as 0, 25, and 50%. This research consisted of the preparation of growing media, spawning and incubation of A. auricula, and harvesting of fruit bodies. The success of mushroom cultivation is seen from the value of biological efficiency in each treatment. The combination treatment of 8 days composting time with the addition of 50% rice husk resulted in A. auricula production as much as 52.37%, while the combination of 4 days composting time treatment and the addition of 25% rice husks resulted in the best production of A. auricula, as it produced the mushroom weight of 340 ± 16.95 g with biological efficiency value as much as 68% for five harvest durations within four months after first watering. The treatment of composting media tie and the addition of rice husks to A. auricularia’s were successfully carried out.AbstrakAuricularia auricula merupakan jamur kayu yang umum dikonsumsi dan diminati oleh masyarakat sebagai bahan pangan karena mengandung nutrisi dan banyak manfaat. Budi daya A. auricula dilakukan dengan menggunakan media tanam dalam kondisi lingkungan yang terkontrol. Penelitian ini bertujuan menentukan kombinasi perlakuan waktu pengomposan dan penambahan sekam padi yang optimum terhadap produksi A. auricula. Perlakuan pada penelitian ini adalah waktu pengomposan media tanam selama 0, 2, 4, 6, dan 8 hari, serta penambahan sekam padi sebanyak 0, 25, dan 50%. Penelitian ini tersusun atas pembuatan media tanam, pembibitan dan inkubasi A. auricula, dan pemanenan tubuh buah. Keberhasilan budi daya jamur dilihat dari nilai efisiensi biologi pada setiap perlakuan. Kombinasi perlakuan pengomposan 8 hari dengan penambahan sekam padi 50% menghasilkan produksi A. auricula sebesar 52,37%, sedangkan kombinasi perlakuan waktu pengomposan 4 hari dan penambahan sekam padi 25% menghasilkan produksi A. auricula terbaik karena menghasilkan berat jamur sebanyak 340 ± 16,95 g dengan nilai efisiensi biologi sebesar 68% selama lima durasi panen dengan waktu empat bulan setelah dilakukan penyiraman pertama. Secara keseluruhan, perlakuan waktu pengomposan media dan penambahan sekam padi pada media pertumbuhan A. auricula berhasil dilakukan.
Pelatihan Hidroponik untuk Penghijauan Kembali di Sekolah Menengah Atas Athalia Utami Karmawan, Listya; Agustinah, Widya; Nita S. Kokali, Yuliana; Kurniawan, Andrea; Livia Thio, Cheryl; Felicia, Felicia
SABDAMAS Vol 2 No 1 (2023): SABDAMAS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Unika Atma Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/sabdamas.v2i1.5013

Abstract

Taman pembelajaran merupakan sarana rekreasi yang dapat menjadi wadah pembelajaran, khususnya mata pelajaran Biologi. Pelatihan hidroponik dilakukan kepada guru-guru Sekolah Athalia. Sekolah Athalia sudah memiliki perangkat hidroponik yang dapat dimanfaatkan oleh guru dan siswa/i untuk pembelajaran. Namun, selama masa pandemi COVID-19 fasilitas ini tidak digunakan secara maksimal karena mayoritas pembelajaran dilakukan secara daring dan tidak ada guru yang memiliki pengetahuan teknik hidroponik yang cukup untuk ditugaskan menjadi penanggung jawab. Perangkat hidroponik yang dimiliki Sekolah Athalia memiliki sistem DFT dengan dua ratus lubang dan diberikan dua buah perangkat wick yang dapat dimanfaatkan kembali. Pelatihan dilaksanakan secara luring yang meliputi teori dan praktik teknik hidroponik yang diharapkan dapat memaksimalkan pemanfaatan fasilitas hidroponik yang telah ada. Hasil dimonitor selama empat minggu di grup whatsapp dan juga dilakukan pengamatan pertumbuhan tanaman selama dua bulan setelah pelatihan mulai dari tinggi daun tanaman dalam cm, jumlah daun dihitung dalam helai, dan bobot tanaman dalam gram. Data tumbuhan diolah menggunakan IBM SPSS. Evaluasi dilakukan dalam bentuk microsoft forms. Terdapat hasil pertumbuhan yang signifikan terhadap tinggi dan jumlah daun pada setiap baki. Ada juga bobot penambahan tanaman, tetapi tidak sesignifikan tinggi tanaman. Dari evaluasi, peserta merasa cukup puas dan memahami pelatihan meskipun tempat, waktu, serta minat peserta mendapat nilai yang tidak begitu tinggi.
Inisiasi Bibit Andaliman (Zanthoxylum acanthopodium DC.) melalui Teknik Kultur In vitro Maria Madelaine Nicole; Yanti; Karmawan, Listya Utami
Keluwih: Jurnal Sains dan Teknologi Vol. 4 No. 1 (2023): Keluwih: Jurnal Sains dan Teknologi (February)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/saintek.v4i1.5599

Abstract

Abstract— Andaliman (Zanthoxylum acanthopodium DC.) is one of the Indonesian spices that is not easy to be cultivated outside its natural habitat because it requires lower temperatures for its growth. This research aimed to determine the culture technique of andaliman plants using in vitro tissue culture techniques to solve the difficulties of plant cultivation outside its natural habitat. The explants used are seeds and nodal segments. Using seeds, methods were divided into seeds collection and sterilization and initiation. Methods using nodal segments explants were divided into sample collection, nodal sterilization, initiation, and shoot multiplication. Seed sterilization using 60 °C water which was replaced every six hours had lower contamination rate compared to other methods. Unfortunately, seeds germination and dormancy breaking were not exhibited in this research. Nodal segment sterilization using 0,1% (b/v) mercury chloride (HgCl2) exposed for seven minutes and addition of 100 IU mL-1 nystatin showed fewer contaminations. Multiplication using Murashige-Skoog (MS) media with addition of 1 ppm NAA and 5 ppm BAP (MSN1B5) produced nine shoots and produced callus. Overall, both explant sterilizations were successful, but it was recommended to use nodal segments as the explant due to success in producing andaliman plantlet in vitro. Keywords: andaliman, HgCl2, nystatin, plant tissue culture, woody plant medium Abstrak— Andaliman (Zanthoxylum acanthopodium DC.) adalah salah satu rempah Indonesia yang sulit untuk dibudidayakan di luar habitat aslinya karena membutuhkan suhu rendah untuk pertumbuhannya. Penelitian ini bertujuan untuk menentukan teknik perbanyakan tanaman andaliman menggunakan teknik kultur jaringan secara in vitro dengan memperhatikan beberapa faktor seperti larutan sterilisasi yang digunakan dan zat pengatur tumbuh (ZPT) yang diberikan. Eksplan yang digunakan adalah biji dan nodus. Metode penelitian dengan eksplan biji meliputi koleksi buah, germinasi, dan inisiasi eksplan. Metode penelitian dengan eksplan nodus dibagi menjadi koleksi bibit, inisiasi nodus, dan multiplikasi nodus. Sterilisasi permukaan biji menggunakan air hangat bersuhu 60 °C yang diganti setiap enam jam memiliki tingkat kontaminasi yang paling rendah dibandingkan dengan metode lainnya, tetapi germinasi dan pemecahan dormansi tidak terjadi pada penelitian ini. Sterilisasi permukaan nodus menggunakan larutan merkuri klorida (HgCl2) 0,1% (b/v) dengan durasi perendaman selama tujuh menit dan 100 IU mL-1 nistatin pada media menunjukkan tingkat kontaminasi yang lebih rendah. Multiplikasi menggunakan media Murashige-Skoog dengan penambahan 1 ppm NAA dan 5 ppm BAP (MSN1B5) menghasilkan organ tunas dalam jumlah yang paling banyak yaitu sebanyak sembilan tunas dan terbentuk kalus. Secara keseluruhan, sterilisasi kedua eksplan berhasil dilakukan, tetapi lebih disarankan menggunakan eksplan nodus karena dapat menghasilkan tunas andaliman secara in vitro. Kata kunci: andaliman, HgCl2, kultur Jaringan tanaman, nistatin, woody plant medium
Revitalisasi Hidroponik sebagai Sarana Belajar di SMP Athalia, Tangerang Selatan, Banten Wulandari, Yasinta Ratna Esti; Hutagalung, Rory Anthony; Chow, Aozora Valentinus; Hartono, Celine Wijaya; Theja, Serafina; Karmawan, Listya Utami
Abdimas Galuh Vol 6, No 2 (2024): September 2024
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v6i2.13998

Abstract

Hidroponik dapat menjadi salah satu sarana pembelajaran tambahan bagi para siwa/i untuk mencapai pembangunan berkelanjutan/sustainable development goals (SDGs). Dengan adanya kegiatan pengabdian kepada siswa/i SMP Athalia yang dilakukan oleh tim dosen Fakultas Teknobiologi bersama mahasiswa, perangkat hidroponik yang tadinya tidak dapat dimanfaatkan karena tanaman terkena air hujan sehingga menyebabkan tanaman merunduk dan minimnya pengetahuan terkait hidroponik dapat teratasi. Pelatihan dilaksanakan secara luring yang meliputi teori dan praktik teknik hidroponik yang diharapkan dapat memaksimalkan pemanfaatan fasilitas hidroponik yang telah ada. Hasil monitor selama empat minggu dengan menggunakan tanaman sawi hijau, bayam merah, dan sawi pagoda dengan parameter tinggi daun, jumlah daun, dan bobot basah tanaman menghasilkan bayam merah memiliki pertumbuhan terbaik dengan bobot basah sebesar 17,26 gram. Evaluasi dan kuesioner kegiatan dilakukan dalam bentuk Microsoft Forms. Evaluasi pengetahuan serta pemahaman dari para siswa/i menunjukkan perubahan signifikan yang dapat dilihat dari peningkatan jumlah jawaban benar para siswa/i yang sebelumnya kurang tepat menjadi hampir keseluruhan siswa/i benar. Keseluruhan kegiatan workshop sudah berlangsung dengan cukup baik dan memadai, baik dalam hal perlengkapan, kesiapan, persiapan, maupun dari segi panitia.