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Aristoteles
Universitas Muhammadiyah Ahmad Dahlan Palembang
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Public Health

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Pemanfaatan Buah Bit (Beta vulgaris L.) sebagai Pengganti Eosin pada Pewarnaan Papanicolaou Lisa Ramadanti; Indah Sari; Aristoteles; Nyiayu Fauziah Kurniawati
jurnal kesehatan terapan sains dan teknologi Vol 4 No 2 (2026): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v4i2.105

Abstract

Background: The pleura is a serous membrane that folds on the surface of the lungs to form a two-layer membranous structure. The gold standard in establishing the diagnosis of pleural effusion is cytological examination. An important stage in cytological examination is the staining process, where the staining technique uses eosin. Eosin as a dye component is carcinogenic and poses a risk to health and the environment if used in the long term. Beetroot containing red pigment, which is a betacyanin compound, was developed as a natural alternative to eosin. Research Objective: This study aims to determine the use of Beetroot (Beta vulgaris L.) as a substitute for eosin in Papanicolaou staining. Research Method: This is an experimental study with an Intact Group Comparison design consisting of two treatment groups, namely staining using eosin and beetroot. Data analysis was carried out using descriptive statistics. This study was conducted at the Barokah Palembang anatomical pathology laboratory on March 16-17, 2025. The population taken was pleural effusion fluid preparations located at the Barokah Palembang anatomical pathology laboratory, the samples used in this study amounted to 32 samples calculated using the Federer formula. Results: : The results of staining using eosin showed that the quality of the preparation was 100% very good, while for staining using beetroot, the quality of the preparation was not good at 18.7%, less good at 50.0%, and good at 31.2%. Conclusion: Alternative staining beetroot can be made into a coloring alternative to the eosin substitute in Papanicolaou coloring.
Perbedaan Nilai Glukosa Pada Tabung Serum Clot dan Tabung Clot Gel Setelah Ditunda Selama 6 Jam Ayu Lestari; Denny Juraijin; Aristoteles; Nurhidayanti
jurnal kesehatan terapan sains dan teknologi Vol 4 No 2 (2026): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v4i2.109

Abstract

Background: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. Delays in blood sample processing can induce glycolysis, leading to decreased serum glucose concentration; thus, the choice of blood collection tube is critical. Objective: To determine the difference in serum glucose levels between Clot Activator Tubes and Serum Separator Tubes (SST) after a 6-hour storage delay. Methods: This quasi-experimental study was conducted at RS Bunda Palembang from June 12 to 19, 2025. A total of 42 specimens were selected using purposive sampling. Glucose levels were evaluated immediately and compared with those measured after a 6-hour delay across both tube types. Results: The mean glucose level tested immediately was 202.04 mg/dL. Following a 6-hour delay, the mean glucose level dropped to 182.50 mg/dL in Clot Activator Tubes and 190.16 mg/dL in SSTs. Statistical analysis revealed a significant difference in glucose stability between the two tube types (p < 0.001). Conclusion: There is a significant difference in serum glucose levels between Clot Activator Tubes and Serum Separator Tubes after a 6-hour delay, with SSTs demonstrating superior performance in minimizing glucose degradation.
Co-Authors Ayu Lestari Denny Juraijin Indah Sari Lisa Ramadanti Nurhidayanti Nyiayu Fauziah Kurniawati