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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal Kesehatan Saelmakers PERDANA (JKSP) JURNAL MEDIA KESEHATAN Jaringan Laboratorium Medis Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Masker Medika Khidmah: Jurnal Pengabdian Kepada Masyarakat Journal Health Applied Science And Technology (JHAST) Jurnal Inspirasi Kesehatan (JIKA)
Indah Sari
IKesT Muhammadiyah Palembang
Religion Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Pemanfaatan Serum Hemolisis Dengan Penambahan Reagen Anti-Rh Terhadap Kadar Enzim Serum Glutamic Pyruvic Transminase (SGPT) Bastian Bastian; Indah Sari; Helnia Sari; Juwy Trianes
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9745

Abstract

Serum glutamate pyruvate transferase (SGPT) also known as alanine aminotransferase (ALT) is an enzyme found in liver cells and is effective in diagnosing hepatocellular destruction. Examination of SGPT activity using blood serum is often difficult due to insufficient blood volume or lysed serum conditions due to improper collection. This study aims to determine whether there is use of serum hemolysis using anti-rh reagents in the examination of SGPT enzyme levels. This type of research uses an experimental design - static group comparison. The sample will be taken by students of DIV Medical Laboratory Technology IKest Muhammadiyah Palembang. The research was carried out starting from patient preparation, taking examination materials, processing examination materials, analysis and examination results. The results of the ANOVA test showed that the significant value was p = 0.000. The p value obtained was p> 0.005. The results of the study found that there was a significant difference in the amount of SGPT in normal serum and serum with the addition of anti-Rh to hemolysis serum
Perbedaan Hasil Pemeriksaan Kromium Heksavalen (Cr VI) Dengan Pengompleks 1,5 Difenilkarbazida Pada Waktu 5 Menit Dan 15 Menit Secara Spektrofotometri UV-VIS Indah Sari; Pra Dian Mariadi; Rosnita Sebayang
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9567

Abstract

Differences of hexavalent chromium (Cr VI) examination results with complexing of 1,5 diphenylcarbazide at 5 minutes and 15 minutes by spectrophotometry uv-vis at (Balai Riset dan Standard Industri Palembang) Research and Industry Standard Center Palembang, 2017. Study Program of DIV Health Analyst, Faculty of Health Sciences of Musi Charitas Catholic University Palembang. Chromium (VI) is a carcinogenic component for humans, the amount of chromium that is safe to consume by humans is 50 - 200 μg a day. This research was an analytical comparative, conducted in The Research and Industry Standard Center Palembang. Samples were from pro-analytical chemical content made from chromium-acting susceptibility of 0,1 ppm and 0,2 ppm and the samples were given a complex of 1,5 diphenylcarbazide in the period of 5 minutes and 15 minutes. The obtained chromium examination results with complexing 1,5 diphenylcarbrazide in the period of 5 minutes were 0,0869 and 0,1775 and in the period of 15 minutes were 0,0932 and 0,1626. The data analyzed by using Wilcoxon p value and it was obtained p> 0,05. Based on this research, it was concluded that chromium examination with complexing of 1,5 diphenylcarbrazide in the period of 5 minutes and 15 minutes had no significant difference.
Perbedaan Kadar Trigliserida Menggunakan Serum dan Plasma Edta dengan Biosystem A15 Sarah Aminah; Indah Sari; Bastian Bastian
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8402

Abstract

Trigycerides are the main lipid component in food intake, which is about 98% of total lipids and the remaining 2% consists of phospholipids and cholesterol. Triglycerides are present in adipose tissue, skeletal muscle, liver, lungs, and intestines to  provide energy in metabolic processes. Who estimates about 1 bilion individuals are overweight and about 300 milion individuals are defined as obese, normal triglyceride levels usually do not exceed 150 mg/dl, if triglyceride levels increase by more than 200 mg/dl then called hypertriglyceridemia. The research aims to determine the difference in triglceride levels using serum and plasma EDTA conducted at the Center For Health Laboratories (BBLK) Palembang. The type of research used is cross Sectional with research designintact group comparison. The sample consisted of 30 serums and 30 plasma EDTA taken from 30 respondents. Research is carried out starting from the preparation of patients, taking examination materials, processing of examination materials, analysis and results of research. The average levels of triglyceride examination using serum and plasma were obtained consecutively at 0.91 mmol/L and 0.83 mmol/L. Mann Whitney test results found that the significant value was p = 0.240. The p value obtained is p0.05. The results can be concluded that there is no difference in examination of triglyceride levels using serum and plasma EDTA.
Education on The Identification of Evidence of Blood Spots Against the Determination of Blood Type Indah Sari; Bastian Bastian; Veny Tresia Utari
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2022): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.428 KB) | DOI: 10.35568/abdimas.v5i1.2078

Abstract

Knowing a person's blood type is very important to know for medical purposes. Blood type is important to know, for the sake of transfusion, the right donor and identification in forensic medical cases such as identification in some criminal cases. Blood stains have an important role in revealing various legal and criminal cases. In the process of identifying bloodstains as evidence, the first thing that must be done is to determine whether the bloodstains actually contain blood molecules so that the blood group can then be identified. Blood group identification is generally done based on the ABO blood grouping system. There have been many criminal cases that have been revealed through the ABO blood group identification system. Blood type examination is one of the methods used when a crime case occurs in the community. There are 4 human blood groups (A, B, AB, and O) which are grouped based on the presence or absence of antigens on the surface of erythrocytes. Identification of blood group ABO system on fresh blood samples is easier to do than dried blood samples. This is because the cells in the dried blood are damaged. However, it is still possible to identify the blood group of dry blood samples. This is because the antigens on the surface of red blood cells remain intact even though the cells have been destroyed. The method commonly used to identify the blood group of dry blood samples is the elution absorption method. This method is a very sensitive method and can detect the presence of antigens indirectly.
Analisis Aktivitas Fisik Ringan dan Berat Terhadap Kadar Hemoglobin Heriyanto,; Indah Sari,; Aristoteles; Bastian
Jurnal Kesehatan Saelmakers PERDANA (JKSP) Vol. 5 No. 1 (2022): Jurnal Kesehatan Saelmakers PERDANA
Publisher : Fakultas Ilmu Kesehatan Universitas Katolik Musi Charitas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32524/jksp.v5i1.406

Abstract

Physical activity is all body movements caused by skeletal muscles with the need for energy expenditure. Physical activity is divided into 3 categories. Light, moderate and heavy physical activity. Heavy physical activity will increase metabolic activity, the acid produced in the form of hydrogen ions and lactic acid will increase a lot, this will cause a decrease in pH and intravascular hemolysis. The affinity between oxygen and hemoglobin will decrease when the blood pH is low. Hemoglobin is a tetrameric protein of erythrocytes that binds to non-protein molecules, namely iron porphyrin compounds called heme. This study aims to determine the effect of light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang. This type of research is carried out in the form of experiments which are analyzed descriptively quantitatively and presented in the form of simple tables and diagrams. The sample was examined using a Hematology Analyzer with the Alfa Swelab Plus brand, the Pothomertic method. The results showed that the average hemoglobin level for light physical activity was 15.01g/dL and heavy physical activity was 15.2 g/dL with the One Way Anova test, the value = 0.689. The conclusion of the study showed that there was no effect between light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang.
PERBEDAAN HASIL FIKSASI ALKOHOL 96% SELAMA 15 MENIT DAN 30 MENIT PADA PEWARNAAN PAPANICOLAOU Ajeng Sukma Rima Dani; Indah Sari
Journal of Indonesian Medical Laboratory and Science Vol 3 No 2: Oktober 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i2.84

Abstract

Cervical cancer is a cancer that apprears in the cervix caused by Human Papilloma Virus (HPV) infection. According to the World Health Organization (WHO) explains that cervical cancer is the fourth-order cancer that represents 6,6% of all cancers is women. Pap smear is an examination done to detect cervical cancer. Pap smear examination using vaginal swab examinations material that must be fixated before coloring papanicolaou. This study aims to determine the defference in alcohol fixation result 96% for 15 minutes and 30 minutes using papanicolaou coloring conducted at Dyatnitalis Anatomy Pathology Laboratory Palembang. Type of cross sectional research, with research design using intact group comparison. The sample consisted of 8 respondents each performed a fixation of alcohol 96% for 15 minutes and the next 30 minutes colored with the coloring papanicolaou. Research is conducted starting from patient preparations, examination material retrieval, examination material processing, analysis and examination result. Wilcoxon test results are known that the significant value is p = 0,024. The value obtained is p =? 0.05.The results can be concluded that there is a difference in alcohol fixation results 96% for 15 minutes and 30 minutes.
PERBEDAAN HASIL KADAR FORMALIN PADA SAMPEL TAHU YANG DIRENDAM AIR HANGAT DAN AIR GARAM MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Indah Sari; Robiyatul Adawiyah; Nurhidayanti Nurhidayanti
JURNAL MEDIA KESEHATAN Vol 15 No 2 (2022): Jurnal Media Kesehatan Poltekkes Kemenkes Bengkulu Volume 15 No 2 Desember 2022
Publisher : Politeknik Kesehatan Kemenkes Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33088/jmk.v15i2.857

Abstract

Problems: Formalin Examination is use to determine of presence or absence of harmful substances or preservatives in samples tested or examined on food, especially tofu. The Aim Of The Research: To determine the difference in the results of formaldehyde levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method. Research Method: The type of research used was a pure experiment, which was conducted at the Laboratory of Chemistry and Microbiology of Agricultural Products, Department of Agricultural Technology, Faculty of Agriculture, Unsri Palembang. The sample used was tofu and the number of careful samples was 18 tofu samples using the total sampling technique. The Result: Based on the results of examining the formalin content in tofu samples soaked in warm water for 15 minutes using the UV-Vis Spectrophotometry method with the A & E Visible Spectrophotometer yielded an average of 0.56 mg/L while the results of examining formalin levels in tofu samples soaked in salt water produces an average of 0.74 mg/L. Data analysis using the Independent Sample T-Test test obtained a significant value for the warm water treatment p = 0.395 and a significant value for the salt water treatment p = 0.397. Conclutions: There are differences in the results of formalin levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method.
PERBANDINGAN KUALITAS SEDIAAN TELUR CACING Trichuris trichiura MENGGUNAKAN PEWARNA EOSIN DAN PEWARNA PERASAN KULIT BUAH MANGGIS Nurhidayanti Nurhidayanti; Kamila Rahmadila; Indah Sari
Masker Medika Vol 11 No 1 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i1.536

Abstract

Pendahuluan: Trichuris trichiura merupakan salah satu jenis cacing yang berasal dari golongan Soil Transmitted Helminth. Trichuriasis adalah infeksi parasit usus yang sering terjadi dan masih menjadi salah satu penyumbang penyakit global. Diagnosis penyakit Trichuriasis dapat dideteksi dengan pemeriksaan feses secara mikroskopis yang dapat dilakukan menggunakan pewarnaan Eosin 2% yang merupakan gold standart untuk pemeriksaan. Penelitian ini bertujuan untuk mengetahui perbedaan hasil kualitas sediaan telur cacing menggunakan pewarna Eosin 2% dan pewarna air perasan kulit buah Manggis. Metode Penelitian: Jenis penelitian yang dilakukan True Eksperimental. Penelitian dilakukan pada tanggal 02-04 januari 2022 di Laboratorium Hematologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang yang berjumlah 32 preparat. Hasil: Hasil yang didapat pada penelitian ini adalah pada pewarnaan Eosin 2% dan pewarnaan air perasan kulit buah Manggis terdapat 5 tingkatan kualitas yaitu Sangat Baik, Baik, Cukup Baik, Tidak Baik, dan Sangat Tidak Baik. Pewarna Eosin 2% mendapat hasil sangat baik sebanyak 2 sediaan, baik sebanyak 4 sediaan, hasil cukup baik sebanyak 5 sediaan, hasil tidak baik sebanyak 4 sediaan dan hasil sangat tidak baik 1 sediaan sedangkan pada pewarnaan air perasan kulit buah Manggis, diperoleh hasil yaitu hasil sangat baik sebanyak 1 sediaan, hasil baik 3 sediaan, hasil cukup baik 4 sediaan, dan hasil tidak baik 4 sediaan dan sangat tidak baik 4 sediaan. Hasil data penelitian dilakukan menggunakan analisa data secara deskriptif. Kesimpulan: didapatkan hasil pemeriksaan bahwa tidak terdapat perbedaan signifikan pada pewarna Eosin 2% dan pewarna air perasan kulit buah Manggis.
PERBEDAAN HASIL PEMERIKSAAN RETIKULOSIT SEBELUM DAN SESUDAH AKTIVITAS FISIK Dewi Hartati; Indah Sari; Zahra Tulhaya Jannah
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.2

Abstract

Introduction: Reticulocytes are young blood cells that have just been released into the bloodstream from the bone marrow. Reticulocytes have characteristics that still have ribosomal RNA remnants. Physical activity is a body movement produced by skeletal muscles and requires energy, including activities performed while working, playing, doing household chores, exercising, and traveling.. Purpose: The purpose of this study was to determine the differences in the results of reticulocyte examination before and after physical activity. Research Method: This research was conducted in 2 laboratories, blood sampling at the Hematology Laboratory, Faculty of Science and Technology, Muhammadiyah Palembang Institute of Health Sciences and Technology, and at the Palembang Health Laboratory Center. The number of samples used was 17 with 2 treatments using purposive sampling technique. Based on the results of reticulocyte examination before and after physical activity using the sysmex-XN1000 hematology analyzer. Results: The results showed that before physical activity produced an average of 1.24% while those after physical activity produced an average of 1.12%. In this study, there was a decrease that was not far from its normal value. Analysis of the data using the Wilcoxon test results obtained sig 0.209 where p≥0.05. Conclusion: The conclusion of this study is that there is no difference between before and after physical activity.
Perbedaan Kadar Hemoglobin Segera Diperiksa dan Penundaan 2,5 Jam Pada Suhu Ruang Indah Sari; Ema Karlina; Bastian
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.5

Abstract

Hemoglobin is a protein molecule in red blood cells that functions as a means of transporting oxygen (O2) from the lungs to tissues throughout the body and taking carbon dioxide (CO2) from the tissue below to the lungs to be released into the free air. This study aims to determine the effect between blood immediately examined and a delay of 2.5 hours at room temperature on hemoglobin levels conducted at IKesT Muhammadiyah Palembang. The type of research used is cross sectional with intact group comparison research design. The sample consisted of 30 samples immediately examined and 30 samples with a delay of 2.5 hours taken from 30 respondents. The study was conducted starting from patient preparation, taking examination materials, processing examination materials, analysis and research results. The average hemoglobin level immediately examined was 12.8 g/dL while the hemoglobin level with a delay of 2.5 hours was 12.7 g / dL, the results of the wilxocon test showed that the significant value was p=0.342. The p value obtained is p>0.05. The results can be concluded that there is no difference in hemoglobin levels immediately examined and a delay of 2.5 hours at room temperature.
Co-Authors Ajeng Sukma Rima Dani Anita Aristoteles, Aristoteles Bastian Bastian Bastian Bastian Dewi Hartati Ema Karlina Helnia Sari Heriyanto, Juwy Trianes Kamila Rahmadila Nurhidayanti Nurhidayanti Nurhidayanti Nurhidayanti, Nurhidayanti Pra Dian Mariadi Robiyatul Adawiyah Rosnita Sebayang Rossa Veronneca Sarah Aminah Veny Tresia Utari Zahra Tulhaya Jannah