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All Journal Jurnal Ilmu Dasar AL KAUNIYAH Cropsaver : Journal of Plant Protection Humanika JURNAL MEDIA KESEHATAN Indonesia Natural Research Pharmaceutical Journal (INRPJ) Journal of Agriculture and Applied Biology Journal of Agriculture and Applied Biology ( JAAB) Jurnal Kefarmasian Indonesia
Sogandi, Sogandi
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences

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Antioxidant Activity of Endophytic Bacterial Extract Isolated from Clove Leaf (Syzygium aromaticum L.) Sogandi, Sogandi; Triandriani, Widya; Saputri, Dina; Suhendar, Usep
Journal of Agriculture and Applied Biology Vol 1 No 1 (2020): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.01.01.02

Abstract

Clove (Syzygium aromaticum L.) is one type of spice plant that has high antioxidant content because of the high eugenol compounds. However, iso-lating these bioactive compounds require a significant amount of biomass. A known alternative solution to overcome the problems is through the utiliza-tion of endophytic bacteria in clove leaves. Endophytic cloves leaf bacteria can be a good antioxidant resource. Endophytic bacteria are bacteria that grow in plant tissues that is not pathogenic, which can replace these plants. The aim of this study is isolating endophytic bacterial from clove leaves, screening endophytic bacteria, determining potential endophytic bacteria as producer antioxidant compounds, and identifying potential isolate using 16S rRNA. This study used endophytic bacteria isolated from clove leaves (Syzygium aromaticum L.). Antioxidant activity determined using DPPH method. It was founded 7 isolates of endophytic bacteria namely WDY1, WDY2, WDY3, WDY4, WDY5, WDY6, and WDY7. The isolate WDY6 shows the highest antioxidant activity. WDY6 isolate is identical to Staphylococcus sp. with a 100% similarity. Staphylococcus sp. has the highest antioxidant activ-ity in the stationary phase. The content of chemical compounds from WDY6 isolates is pyrazine that belongs to alkaloids.
Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia Sogandi, Sogandi; Riyanto, Julius
Journal of Agriculture and Applied Biology Vol 1 No 2 (2020): Journal of Agriculture and Applied Biology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jaab.01.02.03

Abstract

Endophytic bacteria are beneficial microorganisms living in the tis-sues system of various parts of a plant, including fruits, leaves, twigs, and roots. The bacteria are stated as forming colonies without caus-ing any damage to the plant. Thus, this study aimed at isolating endo-phytic bacterial from the leaves, barks, and fruits of Kasturi plant (Mangifera casturi), screening its endophytic bacteria, determining the potential of those endophytic bacteria, identifying 16S rRNA and analyzing potential growth of the bacteria. The isolated endophytic bacteria appeared to show potential activity against pathogenic bac-teria Propionibacterium acnes with disc-diffusion methods. Besides, the observations on bacterial activities showed isolate L2, S2 and F4 isolated from leaves, bark and fruits, respectively, as the most potent producers of antibacterial compounds. Technically, those activities were indicated by the formation of clear zones. Molecular identifica-tion was investigated by applying PCR amplification on 16S rRNA gene. Furthermore, the isolate L2 was identified as Enterobacter clo-acae with 99% sequence similarities; however, isolates S2 and F4 were identified as Escherichia coli. Therefore, these findings sug-gested that the identified strains would contribute to any further searches for new sources of anti-acne substances.
Co-Authors Amelia Amelia Ekajayanti Kining Ginting, Dina Marsella Br. Nilasari, Putu Nur Ilmi Nurul Mukhlisah, Nurul Riyanto, Julius Saputri, Dina Triandriani, Widya usep suhendar