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Ajeng Sandra Allyza
Sekolah Tinggi Kesehatan Al-Fatah Bengkulu
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Method Validation and Analysis of Vitamin C Content in Facial Brightening Creams Distributed in Bengkulu City Using UV-Vis Spectrophotometry Ajeng Sandra Allyza; Herlina Herlina; Luky Dharmayanti; Elly Mulyani
Student Scientific Journal Vol 4 No 4 (2026): October
Publisher : Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37676/ssj.v4i4.12793

Abstract

Vitamin C is widely used in facial brightening creams as an antioxidant, collagen-supporting agent, and inhibitor of melanin formation, yet the actual amount in marketed products is not always quantitatively declared. To determine vitamin C levels in five facial brightening creams marketed in Bengkulu City and to evaluate the validity of the UV-Vis spectrophotometric method. Samples were purposively selected based on BPOM authorization and vitamin C labeling. Qualitative identification used methylene blue, FeCl3, and KMnO4, while the quantitative analysis was performed using UV-Vis spectrophotometry. Validation covered linearity, precision, accuracy, LOD, and LOQ. All samples tested positive for vitamin C. Mean levels in samples A-E were 0.9010%, 0.1673%, 8.0605%, 7.1276%, and 0.00785%, respectively. The mean linearity R² was 0.99960, %RSD was 1.76044%, recovery ranged from 97.999% to 102.451%, LOD was 0.32812 ppm, and LOQ was 0.99430 ppm. The UV-Vis spectrophotometric method met the evaluated validation parameters and was suitable for determining vitamin C in facial brightening cream matrices under the study conditions.
Co-Authors dharmayanti, luky dharmayanti Herlina Herlina Mulyani, Elly