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INFESTASI MONOGENEA PADA IKAN NILA (Oreochromis niloticus) DARI DESA GENUK, UNGARAN BARAT DAN IKAN LELE (Clarias gariepinus) DARI KP. NGLARANG, GUNUNGPATI, JAWA TENGAH Laia, Nilam Permata; Desrina, - -; Haditomo, A.H. Condro
Journal of Aquaculture Management and Technology Volume 7, Nomor 1, Tahun 2018
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Monogenea merupakan salah satu jenis parasit yang sering menginfestasi ikan dan biasanya ditemukan di organ eksternal seperti, kulit, sirip dan insang dengan menancapkan haptor. Parasit monogenea memiliki bentuk tubuh fusiform dan haptor pada bagian posteriori dengan sejumlah kait marginal. Penelitian ini bertujuan untuk mengetahui gejala klinis ikan yang tampak pada ikan nila dan ikan lele sebagai ikan uji, mengetahui nilai intensitas dan prevalensi. Metode penelitian yang digunakan adalah metode random sampling dan pengamatan laboratorium. Ikan yang digunakan yaitu ikan nila dan ikan lele masing-masing sebanyak 50 ekor diambil dari kolam budidaya milik warga. Ikan nila diambil dari Genuk,Ungaran Barat dengan rerata panjang 12.72±1.89 cm dan rerata berat 14.24±2.78 g, sedangkan ikan lele diambil dari Kp. Nglarang, Gunungpati dengan rerata panjang 14.67±1.70 cm dan rerata berat 17.34±3.71 g. Pengamatan monogenea dilakukan dengan pembuatan preparat ulas (smear) yang di ambil dari insang, kulit dan sirip dengan menggunakan cover glass dan diletakkan pada slide glass yang telah ditetesi akuades, kemudian diamati dibawah mikroskop dengan perbesaran 10x, 100x dan 400x. Hasil yang diperoleh dari penelitian ini adalah monogenea yang ditemukan ada dua jenis yaitu Cichlidogyrus sp. pada ikan nila (O. niloticus) dan Actinocleidus sp. pada ikan lele (C. gariepinus). Gejala klinis yang tampak pada ikan nila yaitu sirip punggung dan sirip ekor geripis, operculum kemerahan, insang tampak pucat dan produksi lendir berlebih. Gejala klinis pada ikan lele yaitu pada tubuh terdapat ulcer atau luka, tubuh kemerahan, sirip geripis, sirip kemerahan dan produksi lendir berlebih. Nilai intensitas Cichlidogyrus sp. dan Actinocleidus sp. adalah sama, yaitu 2 ind/ekor, namun nilai prevnalensi keduanya berbeda, prevalensi Cichlidogyrus sp. yaitu 68% dan prevalensi Actinocleidus sp. yaitu 14%.Monogenea is a group of ectoparasites of fish and usually found on the skins, fins and gills with plugging the haptor. Monogenea parasitic had a fusiform body shape on the haptor at posterior with some marginal hooks. This research aims to determind the clinical symptoms of tilapia and catfish, and the intensity and prevalence of monogenea. The methods used in this research were random sampling and laboratory observation. The fish used were tilapia fish and catfish each of many as 50 tails taken from the ponds owned by residents. The tilapia was taken from Genuk, West Ungaran with average length of 12.72±1.89 cm and average weight is 14.24±2.78 g, while the catfish was taken from Kp. Nglarang, Gunungpati with average length 14,67±1,70 cm and average weight 17.34±3.71 g. The observation of monogenea was done started with preparation review (smear) taken from the gills, skin and fins using a cover glass and placed on a slide glass contained a drop of aquadest, the slides was observed under the microscope with maginification 10x, 100x and 400x. There were two monogenea found in this research namely Cichlidogyrus sp. in tilapia fish (O. niloticus) and Actinolcleidus sp. in catfish (C. gariepinus). Clinical symptoms found on tilapia were dorsal and tail fins were torn, reddish operculum, pale gills and excess mucus production. Clininal symptoms in the catfish were wound on body, reddish body, fins were torn and excess mucus production. The value of the intensity of Cichlidogyrus sp. and Actinocleidus sp. was same i.e. 2 ind/tail, however the value of the prevalence of both were different, the prevalence of Cichlidogyrus sp. i.e. 68% and the prevalence of Actinocleidus sp. i.e. 14%. 
PENGARUH PERENDAMAN EKSTRAK DAUN TEMBAKAU (Nicotiana tabacum) TERHADAP KELULUSHIDUPAN DAN HISOTPATOLOGI HATI IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI BAKTERI Aeromonas hydrophila Tarigan, Lidya Aprillia; Desrina, - -; Sarjito, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penyakit Motile Aeromonas Septicemia (MAS) adalah salah satu penyakit yang sering menyerang ikan nila yang disebabkan oleh bakteri A. hydrophila. Penyakit ini mampu mengakibatkan kematian sebesar 80 – 100 % dalam jangka waktu 2 minggu. Salah satu upaya yang dilakukan untuk mengobati serangan bakteri A. hydrophila yaitu menggunakan bahan alami seperti ekstrak daun tembakau. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun tembakau terhadap kelulushidupan dan histopatologi hati ikan nila yang diinfeksi bakteri A. hydrophila. Metode peneletian yang digunakan adalah metode eksperimental dengan 4 perlakuan dan 3 kali ulangan. Dosis ekstrak daun tembakau yang digunakan pada penelitian ini adalah A (0 mg/l), B (250 mg/l), C (500 mg/l) dan D (750 mg/l). Ikan nila yang digunakan sebanyak 120 ekor, kemudian diinfeksi A. hydrophila dengan kepadatan 106 CFU/ml melalui intramuskular. Setelah muncul gejala klinis, dilakukan perendaman menggunakan ekstrak daun tembakau selama 10 menit. Rata-rata kelulushidupan ikan nila yaitu 63,33±25,17 (Perlakuan D), 56,67±15,28 (perlakuan C), 50,00±10,00 (perlakuan B), 20,00±10,00 (perlakuan A). hasil penelitian diperoleh bahwa perendaman ekstrak daun tembakau berpengaruh nyata (P<0,05) terhadap kelulushidupan ikan nila. perlakuan D (750 mg/l) merupakan dosis optimum dengan kelulushidupan tertinggi dan tingkat kerusakan histologi hati paling ringan. The Motile Aeromonas Septicemia (MAS) is the bacterial disease of tilapia caused by A. hydrophila. The disease can to cause 80-100% of mortality within a period of 2 weeks. One of the effort used to control A. hydrophila infection was using  natural ingredient like Tobacco leaf (N. tabacum) extract. This study aims to determine the effect of  tobacco leaf extracts on survival rate and liver histopathology tilapia infected bacteria A. hydrophila. The experiment consisted of 4 treatments, each with 3 replications. doses used in this study were A (0 mg / l), B (250 mg / l), C (500 mg / l) and D (750 mg / l). 120 tilapias were used in this study. The experimental tilapias were infected with A. hydrophila at density 106 CFU/ml through intramuscular. After injection showing clinical signs, tilapias were immersed in N. tabacum extracts about 10 minutes. The average survival rate of tilapia were 63.33 ± 25.17 (Treatment D), 56.67 ± 15.28 (treatment C), 50.00 ± 10.00 (treatment B), 20.00 ± 10.00 (treatment A). The result showed that immersed tobacco leaf extracts had significant effect (P <0.05) of survival rate. Treatment D (750 mg / l) was the best dose with the highest survival rate and the least of histopathology cell changes in the liver.
KEBERADAAN White Spot Syndrome Virus (WSSV) PADA UDANG VANNAMEI (Litopenaeus vannamei) DI PERTAMBAKAN KOTA PEKALONGAN Latritiani, Rusthesa; Desrina, - -; Sarjito, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

White Spot Syndrome Virus (WSSV) merupakan virus penyebab penyakit bintik putih pada udang. Sejak pertama terdeteksi di Taiwan pada tahun 1992, penyakit bintik putih ini telah menyebar secara global diikuti dengan pengaruh sosial-ekonomi yang cukup besar. Tujuan dari penelitian ini adalah untuk mengevaluasi keberadaan WSSV pada udang vannamei (Litopenaeus vannamei) di Pekalongan Utara, Jawa Tengah. Studi kasus ini dilakukan pada November - Desember 2016 menggunakan metode purposive random sampling. Tambak udang yang digunakan sebagai titik pengambilan sampel merupakan tambak yang mempunyai riwayat terserang WSSV. Dari enam belas tambak di Pekalongan Utara dipilih secara acak delapan tambak sebagai titik sampling yang tersebar di tiga desa (desa Degayu, desa Kandang Panjang dan desa Krapyak). Dari setiap tambak diambil 18 ekor udang dengan menggunakan anco. Untuk pemeriksaan udang sampel secara PCR dan histologi menggunakan sistem pooling.Deteksi WSSV dilakukan menggunakan Polymerase Chain Reaction (PCR) dan pengamatan secara histopatologi. Hasil studi ini menunjukkan bahwa tiga udang dari delapan tambak positif terinfeksi WSSV, dengan nilai prevalensi adalah 37,5%. Hasil dari nested PCR menunjukan band 333 bp. Pengamatan histopatologi yang dilakukan pada udang vannamei dilakukan pada organ insang dan hepatopankreas. Terlihat ada beberapa badan inklusi dan hipertrofi pada inti. Kesimpulan studi ini menunjukan bahwa terdapat udang yang terinfeksi ringan WSSV pada budidaya udang vannamei di Kota Pekalongan. White spot syndrome virus (WSSV) is the causative agent of the white spot disease on shrimp. Since firstly detected in Taiwan in 1992, the disease has spread globally and followed with considerable socio-economic consequences. The objective of the present study was to evaluate the presence of WSSV in Litopenaeus vannamei at North Pekalongan, Central Java. This case study was held on November - December 2016 using a purposive random sampling method. The ponds were selected as sampling point is the ponds that has history experience infected by White Spot Syndrom Virus. From sixteen ponds in North Pekalongan were selected randomly eight ponds as sampling point that spread in the three villages (Degayu village, Kandang Panjang village and Krapyak village). From each ponds were selected eighteen shrimps using an anco.  The shrimps that was examined with PCR and histopathological observation used pooling system.This research using nested polymerase chain reaction (PCR) and histopathological observation to detect WSSV. The results of this study showed that there are three ponds were positive WSSV from eight ponds, with the prevalence was 37,5%. The result of nested PCR revealed the bands is 333 bp. Histopathological observation was performed on L. vannamei at gills and hepatopancreas. There are some inclusion bodies and hypertrophy on nucleus. In conclusion, this study showed that there was lightly infectious WSSV on vannamei shrimps in Pekalongan City.
PENGGUNAAN EKSTRAK AKAR TUBA (Derris elliptica) DENGAN DOSIS YANG BERBEDA UNTUK PEMBIUSAN BENIH IKAN NILA (Oreochromis niloticus) DALAM PENGANGKUTAN SISTEM TERTUTUP Prasetyo, Muhammad Deny Haris; Desrina, - -; Yuniarti, Tristiana
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Pengangkutan benih ikan jarak jauh membutuhkan bahan anastesi yang bertujuan menurunkan metabolisme ikan. Salah satu sumber anastesi yang potensial secara alami terdapat di Indonesia adalah akar tuba. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak akar tuba dengan dosis berbeda terhadap kelulushidupan benih ikan nila pada pengangkutan sistem tertutup, mengetahui dosis pemberian ekstrak akar tuba terbaik dan mengetahui pengaruh pemberian anastesi ekstrak akar tuba terhadap profil darah yang terdiri dari leukosit, eritrosit, hemoglobin, hematokrit. Materi yang digunakan pada penelitian ini adalah benih ikan nila ukuran 6-8 cm, ekstrak akar tuba, sterofoam kotak, kantong plastik, mobil pengangkutan, pipet tetes, spuit suntik, dan antikoagulan (EDTA). Metode yang digunakan adalah metode eksperimental. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 4 perlakuan, 3 ulangan dan waktu pengangkutan selama 10 jam. Dosis ekstrak akar tuba yang digunakan adalah perlakuan A: 0 ml/L, B: 0,3 ml/L, C: 0,4 ml/L, D: 0,5 ml/L dengan kepadatan benih ikan 30 ekor/L air. Hasil yang diperoleh menujukkan kelulushidupan benih pasca pengangkutan tertinggi terdapat pada perlakuan B dengan menggunakan dosis 0,3 ml/L yaitu sebesar 70±0,03% dan kelulushidupan terendah terdapat pada perlakuan D dengan dosis 0,5 ml/L yaitu 28±0,01%. Hasil analisa profil darah menunjukkan jumlah leukosit berada pada kisaran normal dan untuk eritrosit, hematokrit, hemoglobin berada dibawah kisaran normal. Berdasarkan hasil penelitian diperoleh kesimpulan bahwa dosis terbaik untuk penggunaan ekstrak akar tuba adalah 0,3 ml/L,  kelulushidupan tertinggi diperoleh sebanyak 70±0,03%. Profil darah leukosit berada pada kisaran normal dan eritrosit, hematokrit, hemoglobin berada dibawah kisaran normal. Pemberian ekstrak akar tuba potensial untuk digunakan sebagai bahan anastesi dalam pengangkutan benih nila sistem tertutup. The transportation of fish seed is done by farmers for long distance. In nature there are still many natural resources that can be used to be anastesi, including tuba roots. Anesthesia is required to make the fish faint during transportation. The material used in this study is 6-8 cm tilapia seeds, tuba root extract, box sterofoam, plastic bag, transport car, drip blade, injection syringe, EDTA bottle. The method used is experimental method. The experimental design used was Completely Randomized Design with 4 treatments, 3 replications, with time 10 hours of transport. The dosage of tuba root extract used was A: 0 ml/L, B: 0.3 ml/L, C: 0.4 ml/ L, D: 0.5 ml / L with 150 fish / fish density. The result obtained is the best life using dose 0,3 ml /L that is 70 ± 0,03 and lowest life with dose 0,5 ml/L that is 28 ± 0,01. As for the leukocyte blood profile in the normal range and for erythrocytes, hematocrit, hemoglobin is abnormal range. The result of the research, it can be concluded that the best dosage for tuba root extract is 0.3 ml/L, the best survival rate is 70±0,03%, leukocyte are in the normal range and erythrocytes, hematocrit, hemoglobin is below the normal. Giving of potential tuba root extract for use as an anesthetic in the transportation of nile tilapia seeds in closed systems.
PENGARUH LAMA PERENDAMAN HORMON TIROKSIN TERHADAP DAYA TETAS TELUR, PERTUMBUHAN, DAN KELANGSUNGAN HIDUP LARVA IKAN MAS KOKI (Carassius auratus) Manurung, Septina; Basuki, Fajar; Desrina, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan Mas Koki (Carassius auratus) merupakan salah satu jenis ikan hias yang sangat digemari masyarakat serta memiliki nilai ekonomis yang sangat tinggi. Namun derajat penetasan dan pertumbuhan ikan mas koki sangat rendah. Salah satu upaya yang dapat dilakukan untuk mempercepat daya tetas, pertumbuhan dan kelangsungan hidup larva ikan mas koki yaitu melalui ransangan hormonal seperti hormon tiroksin. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian hormone tiroksin terhadap HR, SR larva ikan mas koki (C. auratus) dan untuk mengetahui pengaruh lama waktu perendaman hormon tiroksin yang terbaik untuk meningkatkan daya tetas, pertumbuhan serta kelangsungan hidup larva ikan mas koki (C. auratus). Penelitian ini menggunakan metode eksperimental dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yaitu Perlakuan A (control), B (perendaman hormon tiroksin 8 jam dengan dosis 0,10 mg/l), C (perendaman hormon tiroksin 16 jam dengan dosis 0,10 mg/l), dan D (perendaman hormon tiroksin 24 jam dengan dosis 0,10 mg/l). Data yang diamati meliputi daya tetas telur (HR), survival rate (SR), laju pertumbuhan spesifik, dan kualitas air. Hasil penelitian menunujukkan bahwa daya tetas telur ikan mas koki pasca perendaman hormon tiroksin berpengaruh nyata (P≤0,01) dengan hasil terbaik terdapat pada perlakuan D 77.67%±1.15, sedangkan tingkat kelulushidupan serta laju pertumbuhan spesifik juga menunjukkan tidak berpengaruh nyata (P≥0,01). Sedangkan kualitas air selama pemeliharaan masih dalam kisaran layak untuk budidaya ikan mas koki. Goldfish (C. auratus) is one type of ornamental fish that is very popular community and has a very high economic value. But the degree of hatching and growth of the goldfish is very low. One effort that can be done to accelerate the hatchability, growth and survival of goldfish larvae is through hormonal stimulation such as thyroxine hormone. This study aims to determine the effect of thyroxine hormone on HR, SR chef larvae (C. auratus) and to determine the effect of longest time of thyroxine hormone immersion to increase hatchability, growth and survival of goldfish larvae (C. auratus). This research used experimental method with 4 treatments and 3 replications. The treatments in this study were Treatment A (control), B (8 hours of thyroid hormone immersion with a dose of 0.10 mg / l), C (16 hours of thyroxine hormone with a dose of 0.10 mg / l), and D (hormone immersion 24 hours of thyroxine at a dose of 0.10 mg / l). Observed data included egg hatchability (HR), survival rate (SR), specific growth rate, and water quality. The results showed that the hatchability of goldfish eggs after soaking the hormone thyroxine significantly (P≤0,01) with the best results was found in treatment D 77.67% ± 1.15, whereas the survival rate and the specific growth rate also showed no significant effect (P≥ 0.01). While the quality of water during maintenance is still within a reasonable range for the cultivation of the goldfish.   Goldfish (C. auratus) is one type of ornamental fish that is very popular community and has a very high economic value. But the degree of hatching and growth of the goldfish is very low. One effort that can be done to accelerate the hatchability, growth and survival of goldfish larvae is through hormonal stimulation such as thyroxine hormone. This study aims to determine the effect of thyroxine hormone on HR, SR chef larvae (C. auratus) and to determine the effect of longest time of thyroxine hormone immersion to increase hatchability, growth and survival of goldfish larvae (C. auratus). This research used experimental method with 4 treatments and 3 replications. The treatments in this study were Treatment A (control), B (8 hours of thyroid hormone immersion with a dose of 0.10 mg / l), C (16 hours of thyroxine hormone with a dose of 0.10 mg / l), and D (hormone immersion 24 hours of thyroxine at a dose of 0.10 mg / l). Observed data included egg hatchability (HR), survival rate (SR), specific growth rate, and water quality. The results showed that the hatchability of goldfish eggs after soaking the hormone thyroxine significantly (P≤0,01) with the best results was found in treatment D 77.67% ± 1.15, whereas the survival rate and the specific growth rate also showed no significant effect (P≥ 0.01). While the quality of water during maintenance is still within a reasonable range for the cultivation of the goldfish.
PEMBERIAN EKSTRAK KASAR DAUN TEMBAKAU (Nicotiana tabacum) UNTUK MENGENDALIKAN INFESTASI ARGULUS sp. PADA IKAN KOMET (Carassius auratus auratus) Gultom, Dian Sary; Desrina, - -; Sarjito, - -
Journal of Aquaculture Management and Technology Volume 7, Nomor 1, Tahun 2018
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penyakit Argulosis adalah salah satu penyakit yang menyerang ikan komet yang disebabkan oleh parasit Argulus sp. Salah satu upaya pengendalian serangan Argulus sp. yaitu menggunakan insektisida alami. Ekstrak kasar daun tembakau (N. tabacum) merupakan salah satu bahan alami yang dapat digunakan sebagai alternatif pengobatan Argulus sp. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun tembakau terhadap kelulushidupan ikan komet dan mortalitas Argulus sp. Metode penelitian yang digunakan adalah eksperimental dengan 4 perlakuan dan 3 kali ulangan. Dosis yang digunakan pada penelitian ini yaitu perlakuan A (0 ml/L), perlakuan B (3 ml/L), perlakuan C (6 ml/L) dan perlakuan D (9 ml/L). Ikan uji yang digunakan adalah ikan komet sebanyak 60 ekor dengan panjang tubuh rata-rata 6,42±0,45 cm dan bobot tubuh rata-rata 7,61±0,72 gram, kemudian Argulus sp.sebanyak 120 ekor. Uji in vivo dilakukan dengan cara menginfeksikan Argulus sp. dengan kepadatan 30 ekor Argulus sp. ke dalam tiap-tiap perlakuan selama 12 hari, setelah muncul gejala klinis kemudian dilakukan perendaman menggunakan ekstrak kasar daun tembakau selama 60 menit. Pengamatan dilakukan selama 7 hari dengan parameter pengamatan meliputi gejala klinis ikan, kelulushidupan ikan, mortalitas Argulus sp. dan kualitas air. Hasil penelitian menunjukan bahwa pemberian ekstrak kasar daun tembakau tidak berpengaruh nyata (P>0,05) terhadap kelulushidupan ikan komet yang diinfeksi Argulus sp. Nilai kelulushidupan tertinggi terdapat pada perlakuan B sebesar 80,00% diikuti oleh perlakuan C sebesar 73,33%, perlakuan D sebesar 60,00% dan pada perlakuan A sebesar 46,67%. Penggunaan ekstrak daun tembakau terbukti berpengaruh terhadap mortalitas Argulus sp. yaitu 25 ekor (Perlakuan D), 19 ekor (perlakuan C), 12 ekor (perlakuan B), dan 5 ekor (perlakuan A). Hasil penelitian diperoleh bahwa pemberian ekstrak daun tembakau pada perlakuan D (9 ml/L) merupakan dosis yang dapat menyebabkan mortalitas yang tinggi pada Argulus sp. dengan tingkat mortalitas 83,33%.Argulosis is a common disease infectcomet fish caused by Argulus sp.parasites. One of the efforts to control Argulus sp. attack that was used natural insecticide. Tobacco leaf extract (N. tabacum) was one of the natural ingredients that can be used as an alternative treatment of Argulus sp. This study aims to determine of immersed the effect of tobacco leaf extract on survival rate and the effect of immersed of tobacco leaf extract (N. tabacum) on mortality of Argulus sp. The experiment consisted of 4 treatments each with 3 replications. Doses used in this study were treatment A (0 ml/L), treatment B (3 ml/L), treatment C (6 ml/L) and treatment D (9 ml/L). 60 comet were used in this study with an average body length of 6,42±0,45 cm and an average body weight of 7,61±0,72 grams, and then infested by 120 Argulus sp.The experimental comet were infected with Argulus sp. at density 30 Argulus sp.were conducted for 12 days, after injection showed clinical signs comet were immersed in tobacco leaf extract about 60 menit. The observations were conducted for 7 days with observation parameters including clinical symptoms, survival rate, mortality Argulus sp. and water quality. The result showed that immersed tobacco leaf extract had no significant (P>0.05) of survival rate. of comet fish infected by Argulus sp.The highest survival rate in treatment B (80,00%) treatment C (73,33%) treatment D (60,00%) and treatment A46,67%. The result showed that immersed tobacco leaf extracts had significant effect of mortality Argulus sp. is 25 Argulus sp. (Treatment D), 19 Argulus sp. (treatment C), 12 Argulus sp. (treatment B), and 5 Argulus sp. (treatment A). The results showed that immersion in treatment D (9 ml/l) was a dose that could cause high mortality in Argulus with 83,33%.   Argulosis is a common disease infectcomet fish caused by Argulus sp.parasites. One of the efforts to control Argulus sp. attack that was used natural insecticide. Tobacco leaf extract (N. tabacum) was one of the natural ingredients that can be used as an alternative treatment of Argulus sp. This study aims to determine of immersed the effect of tobacco leaf extract on survival rate and the effect of immersed of tobacco leaf extract (N. tabacum) on mortality of Argulus sp. The experiment consisted of 4 treatments each with 3 replications. Doses used in this study were treatment A (0 ml/L), treatment B (3 ml/L), treatment C (6 ml/L) and treatment D (9 ml/L). 60 comet were used in this study with an average body length of 6,42±0,45 cm and an average body weight of 7,61±0,72 grams, and then infested by 120 Argulus sp.The experimental comet were infected with Argulus sp. at density 30 Argulus sp.were conducted for 12 days, after injection showed clinical signs comet were immersed in tobacco leaf extract about 60 menit. The observations were conducted for 7 days with observation parameters including clinical symptoms, survival rate, mortality Argulus sp. and water quality. The result showed that immersed tobacco leaf extract had no significant (P>0.05) of survival rate. of comet fish infected by Argulus sp.The highest survival rate in treatment B (80,00%) treatment C (73,33%) treatment D (60,00%) and treatment A46,67%. The result showed that immersed tobacco leaf extracts had significant effect of mortality Argulus sp. is 25 Argulus sp. (Treatment D), 19 Argulus sp. (treatment C), 12 Argulus sp. (treatment B), and 5 Argulus sp. (treatment A). The results showed that immersion in treatment D (9 ml/l) was a dose that could cause high mortality in Argulus with 83,33%.
STUDI KASUS INFESTASI Cichlidogyrus PADA IKAN NILA (Oreochromis niloticus) DARI SATKER BPBIAT DAN LUAR SATKER BPBIAT JANTI, KLATEN, JAWA TENGAH Kamil, Mia Rahmah; Prayitno, Slamet Budi; Desrina, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Cichlidogyrus merupakan salah satu parasit dari golongan monogenea yang meninfestasi ikan nila. Cichlidogyrus menyerang pada insang yang menempel pada filamen insang dan memakan sel-sel epitel insang, mukus dan darah pada insang. Hal ini dapat menimbulkan kematian bagi ikan-ikan nila yang masih muda atau dalam keadaan lemah akibat stres dan infeksi akut. Penelitian ini bertujuan untuk mengetahui gejala klinis ikan yang terinfestasi Cichlidogyrus, morfologi dari Cichlidogyrus, jumlah, prevalensi, intensitas Cichlidogyrus serta uji statistika perbandingan antara jumlah Cichlidogyrus, prevalensi dan intensitas antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Metode yang digunakan yakni monitoring menggunakan data primer dan data sekunder. Materi yang digunakan yaitu ikan nila sebanyak 252 ekor di ambil secara acak dari kolam Satker BPBIAT dan kolam Masyarakat Desa Janti. Pengamatan ektoparasit dilakukan dengan pembuatan preparat segar dan selanjutnya diamati dibawah mikroskop. Hasil dari penelitian ini yakni gejala klinis yang tampak pada ikan nila yakni berupa produksi lendir berlebih pada insang dan warna insang merah pucat. Cichlidogyrus mempunyai tubuh pipih memanjang, memiliki sepasang mata, faring yang berbentuk bulat, cuping kepala atau cephalic lobe, organ reproduksi, haptor yang terdiri dari 2 pasang jangkar dan 7 pasang kait. Jumlah Cichlidogyrus pada kolam Satker BPBIAT Janti 1169 individu dan 641 individu pada Kolam Masyarakat Desa Janti. Prevalensi Cichlidogyrus pada Satker BPBIAT Janti 65,87% dan kolam Masyarakat Desa Janti 69,04%. Intensitas rata-rata Cichlidogyrus sebesar 12 ind/ekor pada kolam Satker BPBIAT Janti  dan 6 ind/ekor pada kolam Masyarakat Desa Janti. Uji t independen perbandingan jumlah Cichlidogyrus, prevalensi dan intensitas tidak berbeda nyata antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Dapat disimpulkan bahwa dua lokasi tidak berbeda signifikan terhadap jumlah Cichlidogyrus, prevalensi dan intensitas. Cichlidogyrus is one of the monogenean parasites that infestated tilapia. Cichlidogyrus anchored the gill filaments and fed on the epithelial cells, mucus and blood of the gills. This could lead to death for young tilapia or weaken due to stress and acute infection. The aim of this research were to observe the clinical symptoms of Cichlidogyrus infected fish, Cichlidogyrus morphology, infestation, prevalence, and intensity between Satker BPBIAT Janti and Janti Village Community were compared. Monitoring of the both pond sites and interviewed were carried out to abtain primary and secondary data. The material used were 252 tilapia fish taken random by from Satker BPBIAT ponds and Janti Village Community Ponds. Ectoparasite observations were performed by fresh smears  and subsequently observed under a microscope. The results showed that  clinical symptoms appeared in the tilapia were excess of mucus production in the gills and pale gill color. Cichlidogyrus has elongated flat body, a pair of eyes, a round pharynx, a head lobe or a cephalic lobe, a reproductive organ, a haptor consisting of 2 pairs of anchors and 7 pairs of hooks. Number of Cichlidogyrus in Satker BPBIAT Janti were 1169 individuals while in Janti Village Community Ponds were 641 individuals. Prevalence of Cichlidogyrus on Satker BPBIAT Janti was 65,87% and Janti Village Community Pool was 69,04%. Intensity of Cichlidogyrus amounted to 12 ind / tail and 6 ind / tail on Satker BPBIAT Janti and Janti Village Community Ponds respectively. The independent t-test of Cichlidogyrus comparison, in prevalence and intensity have no significant differences. It can be concluded that those two location has no significant difference on Cichlidogyrus infestation, prevalence and intensity.
Co-Authors Fajar Basuki Gultom, Dian Sary Haditomo, A.H. Condro Kamil, Mia Rahmah Laia, Nilam Permata Latritiani, Rusthesa Manurung, Septina Prasetyo, Muhammad Deny Haris Sarjito, - - Slamet Budi Prayitno Tarigan, Lidya Aprillia Tristiana Yuniarti