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All Journal Analit: Analytical And Environmental Chemistry
Mailinda Ayu Hana Margareta
Universitas Negeri Malang

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ELIMINATING SeO2 IN THE KJELDAHL METHOD FOR PROTEIN CONTENT DETERMINATION OF BSA Muhammad Roy Asrori; Mailinda Ayu Hana Margareta; Deni Ainur Rokhim; Surjani Wonorahardjo
Analit : Analytical and Environmental Chemistry Vol. 12, No. 02 October (2026) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/analit.v12i02.263

Abstract

Protein content using the Kjeldahl method was the most analytical method. For supporting the sustainable environment, the method can be modified efficiently. This study evaluated the validation of the modified Kjeldahl method that eliminates SeO2. The validation was included i.e.: precision, accuracy, linearity, Limit of Detection (LoD), and Limit of Quantification (LoQ). The uncertainty value was estimated. The further analysis was cost analysis and analytical GREEnness metric evaluation. The results showed that the modified Kjeldahl method was acceptable. The validation values are precision (0.039785), accuracy (98.84%), linearity (R2 = 0.9940), LoD (0.547%), and LoQ (1.658%). The estimated uncertainty of this method was ± 0.2892% w/w. The modified method was more economical and greener than the conventional Kjeldahl method.
Co-Authors Deni Ainur Rokhim Muhammad Roy Asrori Surjani Wonorahardjo