ELIMINATING SeO2 IN THE KJELDAHL METHOD FOR PROTEIN CONTENT DETERMINATION OF BSA

Muhammad Roy Asrori (Universitas Negeri Malang)
Mailinda Ayu Hana Margareta (Universitas Negeri Malang)
Deni Ainur Rokhim (SMAN 3 Sidoarjo)
Surjani Wonorahardjo (Universitas Negeri Malang)

Analit: Analytical And Environmental Chemistry
Vol. 12, No. 02 October (2026) Analit : Analytical and Environmental Chemistry

Protein content using the Kjeldahl method was the most analytical method. For supporting the sustainable environment, the method can be modified efficiently. This study evaluated the validation of the modified Kjeldahl method that eliminates SeO2. The validation was included i.e.: precision, accuracy, linearity, Limit of Detection (LoD), and Limit of Quantification (LoQ). The uncertainty value was estimated. The further analysis was cost analysis and analytical GREEnness metric evaluation. The results showed that the modified Kjeldahl method was acceptable. The validation values are precision (0.039785), accuracy (98.84%), linearity (R2 = 0.9940), LoD (0.547%), and LoQ (1.658%). The estimated uncertainty of this method was ± 0.2892% w/w. The modified method was more economical and greener than the conventional Kjeldahl method.