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All Journal Biota Spizaetus: Jurnal Biologi dan Pendidikan Biologi Inornatus: Biology Education Journal Organisms: Journal of Biosciences
Sunarti, Riri Novita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Investigation of wastewater quality at Griya Kain Tuan Kentang, Palembang, South Sumatra Saputra, Muhammad Alif; Pusvita, Dwi; Hanifa, Atina Qurba; Sunarti, Riri Novita; Soleha, Siti; Saputra, Andi; Falahudin, Irham
Inornatus: Biology Education Journal Vol. 5 No. 2 (2025): Inornatus: Biology Education Journal
Publisher : Univeritas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/inornatus.v5i2.838

Abstract

Liquid waste from dyeing is the main source of pollution in small industrial areas such as Griya Kain Tuan Kentang, Palembang City, which is famous for its woven and jumputan fabric production. Liquid waste from these activities has the potential to pollute the surrounding environment. Therefore, it is important to evaluate its quality to support ecosystem sustainability and public health. This study aims to analyse the quality of wastewater using physical parameters (temperature, TDS, TSS and brightness), chemical parameters (pH, Cd, Pb, Fe, Cu), and biological (Coliform and Escherichia coli contamination). The type of research used is quantitative research with experimental methods and laboratory tests. Samples from three locations were analysed in the integrated laboratory of UIN Raden Fatah Palembang and directly analysed on site for physical parameters (temperature, TDS and brightness), biological parameters were analysed using the MPN (Most Probable Number) method, and chemical parameters for heavy metals using AAS (Atomic Absorption Spectroscopy). The results of this study provide an overview of the quality of wastewater at Griya Kain Tuan Kentang which has not met the wastewater quality standards according to the Regulation of the Minister of Environment in 2016 for the brightness and TDS parameters at locations 1 and 2 have met the wastewater quality standards, at location 3 the brightness parameters have not met the wastewater quality standards.
Tepung Umbi Gadung (Dioscorea hispida Dennst) Sebagai Alternatif Media Pengganti Media PDA (Potato Dextrose Agar) Halimah, Nur; Apriani, Ike; Sunarti, Riri Novita
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 2 No. 2 (2022): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v2i2.14252

Abstract

Fungus belong to eukaryotic prosthetic organisms that are chemohypertrophic. When studying the properties of fungus, a material is needed as a nutritional source to grow or isolate fungus known as the media. The most commonly used media for fungal culture is instant PDA media. The relatively high price of instant PDA media is a common problem. That's why we need alternative media that are more economical. The aim of this research is to test whether the gadung tuber flour media can be used as an alternative to PDA. Method used in this study is the experimental method and  design used, fully randomized design with 6 treatments and 4 replicates. Concentrations of gadung tuber flour used were 5%, 10%, 15%, 20% and 25%. The data obtained was analyzed with Annova with a confidence level of 95%. The results showed that the grading tuber meal alternative media had a significant effect on the growth of Candida albicans. Fungus can grow in alternative media at a concentration of 5% to 25% with the highest number of colonies at a concentration of 25%, namely 155.3 in 100 µl.AbstrakJamur termasuk organisme prostetik eukariotik yang bersifat kemohipertrofik. Dalam mempelajari sifat-sifat jamur diperlukan suatu bahan sebagai sumber nutrisi untuk menumbuhkan atau mengisolasi jamur yang dikenal dengan media. Media yang paling umum digunakan untuk kultur jamur adalah media PDA instan. Harga media PDA instan yang relatif mahal merupakan masalah yang umum. Untuk itu diperlukan media alternatif yang lebih ekonomis. Penelitian ini bertujuan untuk menguji apakah media tepung umbi gadung dapat digunakan sebagai alternatif pengganti PDA. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dan desain yang digunakan, Rancangan Acak Lengkap dengan 6 perlakuan dan 4 ulangan. Konsentrasi tepung umbi gadung yang digunakan adalah 5%, 10%, 15%, 20% dan 25%. Data yang diperoleh dianalisis dengan Annova dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa grading media alternatif tepung umbi berpengaruh nyata terhadap pertumbuhan Candida albicans. Jamur dapat tumbuh pada media alternatif pada konsentrasi 5% sampai 25% dengan jumlah koloni tertinggi pada konsentrasi 25% yaitu 155,3 dalam 100 µl
Penilaian Kualitas Air Sungai Sekanak dengan Pendekatan Mikrobiologi Nisak, Fitri Hairul; Sari, Mutiara Manda; RA, Ririn Aulia; Dwitanto, Ahmad; Nurseha, Tito; Sunarti, Riri Novita
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.439

Abstract

Sungai merupakan salah satu sumber kehidupan bagi masyarakat yang tinggal di daerah perkotaan. Banyaknya permukiman di sepanjang sungai sangat memengaruhi kualitas air dari sungai. Penurunan kualitas air ditandai dengan perubahan warna air, bau, dan adanya bakteri Coliform fecal (E. coli). Penelitian ini bertujuan untuk menganalisis kualitas air sungai Sekanak berdasarkan faktor fisika, kimia, dan mikrobiologi. Uji laboratorium yang dilakukan untuk mengetahui keberadaan bakteri Coliform yang merupakan indikator pathogen dalam air. Pengambilan sampel air menggunakan metode purposive sampling, dan uji laboratorium dengan menggunakan metode Most Probable Number (MPN). Hasil analisis mikrobiologi pada sampel air sungai sekanak 1-12 menunjukkan nilai indeks 1100/100 mL, 460/100 mL, 240/100 mL, 75/100 mL, 75/100 mL, 1100/100 mL, 64/100 mL, 1100/100 mL, 1100/100 mL, 1100/100 mL, 1100/100 mL, 2400/100 mL. 12 sampel uji menunjukkan hasil yang tidak memenuhi syarat nilai cemaran Coliform fecal (E. coli) sesuai surat keputusan menteri kesehatan Republik Indonesia Nomor 907 / MENKES / Sk / VII / 2002 menyatakan bahwa air minum harus memenuhi persyaratan dengan tingkat kontaminasi 0/100 mL untuk keberadaan Coliform. Data parameter fisika yang meliputi suhu, dan kekeruhan air, serta parameter kimia berupa pH menunjukkan hasil yang lebih besar dibandingkan baku mutu air sungai pada Peraturan Perundangan Republik Indonesia No.22 Tahun 2021 tentang penyelenggaraan perlindungan dan pengelolaan lingkungan hidup. Hal ini menunjukkan bahwa air sungai tidak memenuhi syarat untuk kebutuhan higiene sanitasi.
Potential of Bacteriophages as Non-Alcoholic Antiseptic Hand Sanitizer Sunarti, Riri Novita; Ike Apriani; Septa Ardiyan Muka Rohma; Adam Ramat hidayat; Frety Abelia Azzumar; Salem Ebraiek
Biota Vol 11 No 2 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i2.25232

Abstract

Bacteriophages, or phages, are viruses that can infect and replicate within bacterial cells, such as Escherichia coli. Phages demonstrate a strong ability to lyse host bacteria and exhibit high survivability, making them a promising innovation for use in non-alcoholic antiseptic products, such as hand sanitizer sprays and bacteriophage gels. This study aims to evaluate the effectiveness of bacteriophage-based hand sanitizer sprays and gels in reducing E. coli growth and total microbial colonies on palms, compared to commercial alcohol-based hand sanitizers. The method used in this study is a descriptive quantitative approach using an experimental method, specifically the Hand Sanitizer Spray and Bacteriophage Gel Test as Non-Alcohol Antiseptics. The average total bacterial colonies on male palms for the control treatment, phage gel sanitizer and commercial gel hand sanitizer were 1.95 x 104 CFU/mL; 1.15 x 103 CFU/mL; 2.55 x 103 CFU/mL, respectively,  while on female palms, the values were 2.35 x 104 CFU/mL; 3.05 x 103 CFU/mL; 1.65 x 103 CFU/mL. The average total bacterial colonies on male palms for control treatment, phage sanitizer spray and commercial sanitizer spray were 1.30 x 105 CFU/mL; 2.05 x 103 CFU/mL; 9, 04 x 104 CFU/mL, respectively, while on female palms, the value was 1.58 x 105 CFU/mL; 8.36 x 103 CFU/mL; 8.79 x 104 CFU/mL. The results demonstrated that both bacteriophage hand sanitizer gel and spray significantly reduce bacterial colonies on palms, with phage-based hand sanitizer showing greater efficacy than commercial alcohol-based hand sanitizer.
Co-Authors Adam Rahmat Hidayat Adam Ramat hidayat Amalia, RA. Hoetari Tirta Amelia Andi Saputra Dinda Mutiara Asmara Dwitanto, Ahmad Fatiqin, Awalul Frety Abelia Azzumar Hanifa, Atina Qurba Ike Apriani Ike Apriani, Ike Irham Falahudin, Irham Lestari, Aneke Nisak, Fitri Hairul Nur Halimah Nurseha, Tito Pusvita, Dwi Putri, Nabela Arinda Ra Hoetary Tirta Amellia RA, Ririn Aulia Rukmini Salem Ebraiek Saputra, Muhammad Alif Sari, Mutiara Manda Septa Ardiyan Muka Rohma Soleha, Siti TRI WULANDARI Yolanda Devira