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All Journal Biota INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Biotropical Research and Nature Technology
Nurseha, Tito
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Plant Growth Regulators (PGRs) Study for Root and Bud Induction on Stem Cuttings of Stevia rebaudiana Apriani, Ike; Sonia Adiba; Dawani; Nurseha, Tito; Fatiqin, Awalul; Yessy Velina
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.22022

Abstract

The Stevia plant is used as a low calorie natural sweetener known as"the sweet herb of Paraguay". Stevia leaves a higher level of sweetness than cane sugar. Stevia can be propagated by stem cutting techniques. Soaked of stem cuttings in Plant Growth Regulators (PGRs) auxin can promote the growth and development of Stevia. Research was conducted to determine the effect of commercial PGRs (Rootone-f) and natural PGRs from Shallot extract. This study used a complete randomized design with different concentrations, both Rootone-f (0, 100, 200, 300, 400 and 500 mg/L) and shallot extract (0, 0.5%, 1%, 1.5% and 2%). Observations were made after 6 weeks (42 days) of planting on several growth parameters (height of plant, number or buds, number of leaves, number of roots and length of roots). Commercial PGRs showed a significant effect on growth parameters. While natural PGRs of shallot showed a significant effect on height of growth parameters. The recommended concentration of commercial PGRs for Stevia rebaudiana Bertoni M. stem cuttings was 300 mg/L.
Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Diversitas Vegetasi Riparian: Upaya Pelestarian Ekosistem Perairan Danau Buatan Air Batu Banyuasin, Sumatera Selatan Kharisma, Sully Pudja; Fauzan, Ahmad Rizki; Permitasari, Irma; Nurseha, Tito
Journal of Biotropical Research and Nature Technology Vol. 2 No. 2 (2024): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i2.12706

Abstract

Danau dibentuk oleh banyak proses diantaranya akibat proses erosi hingga galian tambang. Riparian adalah area konservasi yang harus mempertahankan vegetasi aslinya agar tetap terjaga dan tidak rusak oleh aktivitas ilegal manusia. Tujuan dari penelitian ini adalah untukmelihat peranan riparian terhadap vegetasi danau buatan dan menganalisis vegetasi riparian di daerah danau buatan. Pengumpulan sampel dilakukan dengan menggunakan teknik transek garis. Hasil penelitian menunjukkan bahwa vegetasi di danau buatan Air Batu berupa Stenotaphrum secundatum 2 jenis, Lophatherum gracile 40 jenis, Murcurialis annua 2 jenis, Mimosa pudica 12 jenis, Glactia 3 jenis, Macroptilium atropurpureum 13 jenis, Melastoma malabathricum 11 jenis, Acacia mangium Willd 1 jenis, Selaginella denticulate 1 jenis, Dicranopteris linearis 2 jenis dan Hemarthria altissima 16 jenis. Kelimpahan jenis secara berurutan paling tinggi dimiliki oleh rumput bambu (Lophatherum gracile) 3,076923 rumput limpo (Hemarthria altissima) 1,230769, siratro (Macroptilium atropurpureum) 1,putri malu (Mimosa pudica) 0,923077, senggani (Melastoma malabathricum) 0,846154, kacang polong liar (Glactia) 0,230769, steno (Stenotaphrum second atum) 0,153846, merkuri atau jarang (Murcurialis annua) 0,153846, resam (Dicranopteris linearis) 0,153846, paku rawa (Selaginella denticulate) 0,076923 akasia (Acacia mangium Willd) 0,076923. Indeks diversitas riparian danau buatan Air Batu tergolong ke dalam kategori rendah, dengan nilai 0,356983. Berdasarkan hasil, kelimpahan paling tinggi dimiliki oleh rumput bambu (Lophatherum gracile) sedangkan kelimpahan paling rendah dimiliki oleh paku rawa (Selaginella denticulate) dan akasia (Acacia mangium Willd).
Co-Authors Dawani Fatiqin, Awalul Fauzan, Ahmad Rizki Ike Apriani, Ike Kharisma, Sully Pudja Permitasari, Irma Retnaningrum, Endah Serang, Yitro Soleha, Siti Sonia Adiba Syarifah Syarifah, Syarifah Yessy Velina