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Utilization Dishwashing Soap as a Subtitute of Xylol in the Deparaffinization process of Hematoxylin-Eosin Dye: Review Article mamay, Mamay; Mutmaina, Gina Nafsa; Nurahma, Ina Aflaha
Medicra (Journal of Medical Laboratory Science/Technology) Vol 5 No 1 (2022): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i1.1629

Abstract

Deparaffinization in the hematoxylin eosin staining process aims to remove paraffin from the tissue, clean the tissue and maximize dye absorption. Utilization xylol as deparaffinization has a drawback, if inhaled too much it will cause harm to the health of the laboratory staff. Considering this, a safer alternative to xylol is needed, one of which is dish washing soap. This study was conducted to determine the use of liquid dish soap at the deparaffinization stage on the quality of tissue staining results. The research method in this research is a literature review study conducted based on sources from several articles published digitally in the Science Direct, Pub Med, NCBI, Researchgate, Google Scholar with the keywords deparafinization, xylene, dish washing soap and Hematoxylin-eosin. Based on the results of a review of ten journals, the concentration of dishwashing soap used was 1.5%, 1.7%, 2% and 2.5% with the most widely used concentration of 1.7%. The most used deparaffinization time with dish soap was 1 minute, one study used 2 minutes. Of the several differences in deparaffinization time and concentration of dish soap, the results adequacy of nuclear and cytoplasmic staining, clarity, uniformity and crispness of staining were very good in almost all studies, there was no difference in quality with the use of xylol. So it can be concluded that the use of dish soap in the deparaffinization process for Hematoxylin staining showed good staining of all tissues. Dish soap can be used as an alternative agent in deparaffinization of Hematoxylin-Eosin staining.
PENYULUHAN KADAR CRP (C-REACTIVE PROTEIN) PADA PENDERITA TUBERKULOSIS DI KECAMATAN CILAWU SEBAGAI PENANDA ADANYA INFLAMASI Nurisani, Astari; Mamay, Mamay; Mutmaina, Gina Nafsa; Sugiah, Sugiah; Pratama, Wisnu Arya
Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Vol. 2 No. 2 (2023): Ekalaya Journal
Publisher : Nindikayla Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57254/eka.v2i2.57

Abstract

Infection due to tuberculosis bacteria will cause inflammation in the body which will stimulate the emergence of C-Reactive Protein as a biomarker of inflammation. C-Reactive Protein (CRP) is a polypeptide molecule of the pentraxin group which is produced in the liver and controlled by cytokines especially Interleukin-6 (IL-6). CRP is useful for monitoring disease progression. CRP concentration is related to disease severity, infection and treatment effect. The purpose of this community service is to see CRP (C-Reactive Protein) levels in tuberculosis sufferers in the Cilawu sub-district and provide an understanding of CRP examination. The method used is counseling with LCD and examination of CRP levels in tuberculosis patients. The sufferers are collected in a house that has been mutually agreed upon. The results of the activity were that most of those present had CRP examination results on the 30 samples that had been examined, 24 people (80%) had positive results, and 6 people (20%) had negative results. CRP levels in positive tuberculosis patients obtained various results between 6 mg/L to 192 mg/L. Conclusion: the results of this community service illustrate that there is an inflammatory response of CRP to tuberculosis bacterial infection. After carrying out counseling and examining CRP levels, the community, especially tuberculosis sufferers, became aware and interested in carrying out CRP examinations as a form of monitoring the level of infection they were suffering from
THE BLOOD CREATININE LEVELS OF HYPERTENSIVE PATIENTS OF PRODUCTIVE AGE AT THE CIKAJANG COMMUNITY HEALTH CENTER Rismawati, Rismawati; Mutmaina, Gina Nafsa; Sugiah, Sugiah; Nurisani, Astari; Arbila, Meisya
Jurnal Medika Cendikia Vol 11 No 01 (2024): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v11i01.244

Abstract

The incidence of hypertension is on the rise with the passage of time, attributed to the increasing prevalence of unhealthy lifestyle changes. The productive age signifies a stage in the evolution of life where individuals are at the peak of activity, characterized by heightened levels of engagement that tend to result in health issues. Hypertension can lead to organ damage, notably the kidneys, as indicated by elevated levels of creatinine in the blood. The aim of this study is to ascertain the levels of blood creatinine in hypertensive patients of productive age at the Cikajang Community Health Center. This descriptive study analyzes blood creatinine levels in hypertensive patients using the Jaffe Reaction method, with a sample size of 30 respondents obtained through accidental sampling technique. The study's findings reveal that 19 participants (64%) have normal creatinine levels, 10 participants (33%) have high creatinine levels, and 1 participant (3%) has low creatinine levels. The study concludes that the majority of respondents have normal creatinine levels, nearly half of the respondents have high creatinine levels, and a small portion of respondents have low creatinine levels