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All Journal Chemistry Indonesian Journal of Chemical Research Jurnal Teknologi Hasil Pertanian
Ningrum, Riska Surya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Engineering Environmental Science

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Antibacterial Activity of Pineapple Peel (Ananas comosus) Eco-enzyme Against Acne Bacterias (Staphylococcus aureus and Prapionibacterium acnes) Ramadani, Aisyah Hadi; Karima, Rizkya; Ningrum, Riska Surya
Indonesian Journal of Chemical Research Vol 9 No 3 (2022): Edition for January 2022
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2022.9-nin

Abstract

Acne (acne vulgaris) is a skin disease caused by infection or inflammation of the pilosebaceous unit. Staphylococcus aureus and Prapionibacterium acnes was the main actor in the infection. Eco-enzyme that is made from pineapple peel has been stated that have inhibitory activity against gram-positive and negative bacteria, also applied as home industry soap. This study objective as scientific support that Eco-enzyme phytochemical compounds had the antibacterial activity to the acne bacteria. Eco-enzyme was composed of pineapple peel, brown sugar, and water with the ratio of 3:1:10 for 3 months fermentation time. Eco-enzyme was screened of its phytochemical compound and antibacterial activity against S.aureus and P.acnes by dilution with various concentrations (1.5625%, 3, 125%, 6.25%, 12.5%, 25%, and 50% v/v) and various control. The minimum inhibition concentration (MIC) was then tested by diffusion method to determine the inhibition zone with 3 replication using the higher concentration (50%, 75%, 100% v/v). The result showed that eco-enzyme has a clear yellow colour, contains tannin and saponin, MIC of S.aureus and P.acnes bacteria is 50%. The dilution test of eco-enzyme gives the most effective concentration to inhibit S.aureus at eco-enzyme 100% (v/v), contrasted to P. acnes which didn’t show the best inhibition concentration.
Pengaruh Enzim Amilase Brevibacterium sp dan Glukoamilase Terhadap Kemampuan Penjerapanan Minyak Pada Pati Singkong Uzwatania, Fina; Rahma, Putri Ajeng Syahru; Pramasari, Dwi Ajias; Ningrum, Riska Surya; Sondari, Dewi
Jurnal Teknologi Hasil Pertanian Vol 16, No 1 (2023): Februari
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jthp.v16i1.62318

Abstract

Improper oil waste management contributes to environmental degradation, notably water pollution. Because conventional methods for treating oil pollutants are costly and have limited removal efficacy, the use of natural adsorbents is recommended due to their dependability and affordability. The purpose of this study was to see how modified cassava starch affected oil adsorption using two types of enzymes: Brevibacterium sp amylase enzymes derived from Indonesian marine bacteria and commercial amylase enzyme (Dextrozyme® GA).Oil-adsorption degree is applied to several types of oil, including palm oil and olive oil. The findings revealed that the properties of modified starch differed from those of native starch in both physical and chemical terms. The modified starch produced by hydrolysis of the glucoamylase enzyme (Dextrozyme ® GA)had a yield of 80.16 %, reducing sugar content of 0.20 g/L at 24 h, and a particle size of 377 nm, which is lower than the starch hydrolyzed by Brevibacterium sp. In contrast, the degree of oil adsorption in the glucoamylase enzyme (Dextrozyme ® GA) is higher than Brevibacterium sp. Statistical analysis showed that the oil adsorption degree is affected by the type of enzyme, therefore, the modified starch from Brevibacterium sp still needs improvement to be competitive for oil adsorption compared with the modified starch from the glucoamylase enzyme (Dextrozyme ® GA).
Co-Authors Aisyah Hadi Ramadani Dewi Sondari Fina Uzwatania Karima, Rizkya Pramasari, Dwi Ajias Rahma, Putri Ajeng Syahru