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Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam) Isnaeni, Mei; Faidiban, O R; Tethool, Angelina Novita
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 9 No 2 (2019): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veter
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.695 KB) | DOI: 10.30862/jipvet.v9i2.65

Abstract

Red fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3), ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86% to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P <0.01) on motility 0.05). The spermatozoa concentration ranged from 1,600x106 cells / ml to 2,200x106 cells/ml. The spermatozoa concentration of treatment P1 and P2 chickens did not show any significant differences (P> 0.05), nor did among treatments P2, P3 and P4. However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil up to concentrations of more than 2% tend to reduce sperm motility. Keywords: Concentration, Motility, Spermatozoa and Red fruit oil
Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung Inggriani, Kiki; Tethool, Angelina Novita; Lumatauw, Sintje
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.709 KB) | DOI: 10.46549/jipvet.v10i1.67

Abstract

Antioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung” (Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut” (Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability.
Pengaruh Sari Buah Merah (Pandanus conoideus Lam) terhadap Abnormalitas Spermatozoa Ayam Kampung Tethool, Angelina Novita; Ollong, Abdul Rahman; Koibur, Johan Fredrik
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 11 No 2 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i2.107

Abstract

Antioxidants are substances that can prevent the occurrence of free radical antioxidation reactions in lipid oxidation. Red fruit (Pandanus conoideus Lam) is one of the plants endemic to Papua which contain high antioxidant. The aim of this research was to determine the abnormality spermatozoa of ayam Kampung which were received red fruit extract treatment. Eight cokcs were divided into 4 treatments, each treatment was repeated 5 times. The treatment consisted of control (P0) without extract, P1 with 0.5 mL extract/kgBW/day, P2 with 1 mL extract/kgBW/day and P3 with 1.5 mL extract/kgBW/day. The results showed that red fruit extract decreased the percentage of abnormality spermatozoa (P<0.05) compare to control (without red fruit extract). Forms of abnormality obtained was abnormalities on the head, namely swelled head, bent head, head detachement, knotted tail and smaller head, middle abnormalities, namely midpiece detachement and bending at midpiece border and abnormalities of the tail, namely bent tail, knotted tail, tail detachement and curled tail.
Sifat Kuantitatif Ayam Joper yang Dipelihara Kelompok Peternak “Papua Mandiri” di Kabupaten Manokwari Propinsi Papua Barat Rahayu, Bernaddeta Wahyuni Irianti; Manik, Hotlan; Palulungan, John; Tethool, Angelina Novita
Jambura Journal of Animal Science Vol 6, No 1 (2023): Jambura Journal of Animal Science
Publisher : Animal Husbandry Department, Faculty of Agriculture Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35900/jjas.v6i1.22365

Abstract

Sifat kuantitatif yang dimiliki oleh ayam Joper seperti ukuran tubuh merupakan salah satu parameter penting dalam prog pemuliaan ternak khususnya untuk mengetahui tingkat pertumbuhannya.   Penelitian ini bertujuan untuk mengetahui produktivitas ayam yang telah dipelihara selama dua bulan, menggunakan hubungan antara bobot badan dengan ukuran-ukuran tubuh ayam Joper.  Penelitian menggunakan metode deskripsi dengan teknik observasi. Data yang diukur berupa ukuran kuantitatif. Sampel ayam ditentukan secara purposif sampling pada kedua lokasi penelitian sebanyak 66 ekor.  Data yang diperoleh dianalisa secara tabulasi dan korelasi regresi dengan menggunakan SPSS versi 25. Bobot badan ayam Joper umur 2 bulan menggambarkan nilai yang cukup baik, karena memenuhi standar bobot badan ayam, yaitu 1015,5 g. Bobot badan ayam jantan adalah 1113,94 g dan betina dalah 916,36 g. Nilai korelasi pearson dan R square tertinggi adalah korelasi dan regresi panjang kaki terhadap bobot badan, sehingga ukuran panjang kaki dapat digunakan untuk menduga bobot badan ayam Joper pada usia 2 bulan. 
Analisis cemaran mikrobiologis pangan asal ternak pada jajanan anak sekolah dasar: Microbiological analysis contamination of children’s snacks elementary school Inriani, Noveling; Rumetor, Sientje Daisy; Tethool, Angelina Novita
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 14 No. 2 (2024): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v14i2.477

Abstract

Abstract This study examined the microbiological contamination of street food that consumed by children at primary. The study, specifically focused on the detection of total microorganisms such as Escherichia coli, and Salmonella sp. Microbiological analyses is one way to determine the level of hygiene of snack foods. Presence of microbes determined by using Total Plate Count (TPC), detection test was applied to detect E.coli and Salmonella sp test applied detect present of Salmonella sp. Quantitatif and qualitatif methods have been sed with sampel snacks collection were obtained street food at primary schools. TPC test results showed that 6 samples of snacks have been contaminated with microbes with the threshold of the maximum limit. Those snacks, namely G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5 cfu/gr, L2(egg meat ball) 2.3 x 10-5 cfu/gr, M1 (egg roll) 3.8 x 10-5 cfu/gr, N (meatball) 1.1 x 10-5 cfu/gr and Q1 (batagor) 1.1 x 10-5 cfu/gr. Results Salmonella sp test indicated that there were 9 samples positively contaminated with salmonella sp bacteria. Those are D2 (chicken sausage), E1 (egg roll), E2 (chicken meat ball), F2 (chicken meat ball), G1 (batagor), G2 (fried sausage), N (meatball), P1 (nuggets), P2 (chicken meat ball). Escherchia coli detection test results all samples were declared negative. There were 6 school locations (G, K, L, M, N, Q) different snack samples contaminated with microbes, 6 school locations (D, E, F, G ,N, P) snack samples were contaminated with Salmonella sp. Those snacks were declared not recommended for consumption. Keywords: Contamination; E. coli; Microbes; Salmonella sp; Snacks Abstrak Penelitian ini meneliti kontaminasi mikrobiologi pada jajanan yang ditujukan untuk anak-anak sekolah dasar di distrik Manokwari Barat, dengan fokus khusus pada deteksi mikroorganisme total, E. coli, dan Salmonella sp. Salah satu cara untuk mengetahui tingkat higienitas dari pangan jajanan adalah dengan analisis mikrobiologi berupa uji keberadaan mikroba dengan Total Plate Count (TPC), uji deteksi E.coli dan uji Salmonella sp. Sampel jajanan hasil olahan ternak diperoleh dari jajanan yang ada di Sekolah Dasar di wilayah distrik Manokwari Barat. Penelitian ini menggunakan gabungan metode kualitatif dan kuantitatif dengan menggunakan teknik pengambilan sampel yaitu purposive sampling. Hasil uji TPC pada jajanan pangan hasil ternak di Sekolah Dasar distrik Manokwari Barat terdapat 6 sampel yang berada diambang batas maksimum yaitu sampel G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5cfu/gr L2 (pentol telur) 2.3 x 10-5 cfu/gr. M1 (telur gulung) 3.8 x 10-5 cfu/gr, N (pentol bakso) 1.1 x 10-5 cfu/gr dan Q1 (batagor) 1.1 x 10-5 cfu/gr. Hasil pengujian deteksi Salmonella sp terdapat 9 sampel yang positif tercemar bakteri Salmonella sp. D2 (sosis ayam), E1 (telur gulung), E2 (pentol ayam), F2 (pentol ayam), G1 (batagor), G2 (sosis goreng), N (pentol bakso), P1 (nugget), P2 (pentol ayam) sedangkan untuk hasil uji deteksi E. coli semua sampel dinyatakan negatif. Dari hasil penelitian ini dinyatakan bahwa ada 6 lokasi (G, K, L, M, N, Q) telah terkontaminasi mikroba diambang batas dan tidak layak untuk dikonsumsi dan 6 lokasi (D, E, F, G, N, P) telah terkontaminasi Salmonella sp dan dinyatakan tidak layak untuk dikonsumsi. Kata kunci: E. coli; Mikroba; Pangan jajanan; Salmonella sp; Total plate count