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Muntingia Calabura L. Leaves Extract Effect Towards Increment Of Nitric Oxide In Rattus Norvegicus Ivander, Gian; Isbandiati, Endang; Jong, F.X. Himawan H.
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 4 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Introduction: Hypertension is one of the leading health problem in Indonesia. One of the pathophysiology of hypertension is abnormality in nitric oxide production and transport. On the other side, Muntingia calabura L. is a plant that widely distributed in Indonesian and believed to possessed antinociceptive, antioxidant, antihypertension, and antimicrobial effect. Leaves of Muntingia calabura L. comprise of high phenolic compounds which is thought to increase nitric oxide production through the modulation of NOS and ROS inhibition. Aim: To analyze the effect of extract of Muntingia calabura L. leaves toward increment of nitric oxide in Rattus norvegicus. Method: This research is an in vivo experimental study with post-test only control group design, using nitric oxide strips test as measuring instrument. Sample of this experiment is forty Rattus norvegicus divided in five group which is negative control, positive control, treatment 1, treatment 2, and treatment 3. Extract of Muntingia calabura L. leaves with concentration 50, 100, 200 mg/mL given everyday on treatment group and measured every two days until six measurement is acquired. Result: There is increment of NO through administration extract of Muntingia calabura L. leaves in Rattus norvegicus. Conclusion: Highest increment of NO is obtained through administration of Muntingia calabura L. leaves extract at 200 mg/mL concentration.

Visual Inspection With Acetic Acid (Via) Positive Results And Passive Smoker In Puskesmas Wonoayu Sidoarjo Ursia, Catharina C; Warsanto, JA; Isbandiati, Endang
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 2 (2020): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Introduction: Cervical cancer is a malignancy in women with the number one mortality and number two morbidity in the world. As many as 77% cases are found in developing countries, including Indonesia. Although deadly, screening programs can reduce the morbidity and mortality of this malignancy. Visual Inspection with Acetic Acid (VIA) is a cervical premalignancy screening method used in primary health care in Indonesia. Smoking is one of risk factor associated with cervical cancer and data from GATS, Infodatin 2015 & Riskedas 2013 states that the number of passive smokers in Indonesia is high. Aim: The objective of this research is to look for the correlation between history of passive smoker and positive VIA Methods: This research is a retrospective cross sectional analytic study. The data used in this study is the medical records of VIA screening visitors in 2016. The data were extracted on the research questionnaire. Data processing is done by using SPSS Statistics 23 For Windows computer software. The test is Chi Square correlation test and calculation of Prevalence Ratio (PR). Result: 55 women (24.7%) had a history of passive smoking (daily cigarette smoke exposure), and 168 women (75.3%) had no daily cigarette smoke. 21 women (9.4%) received positive results, and 202 women (90.6%) received negative IVA. In the Pearson Chi-Square section, the Asymptotic Significance (2-sided) yielded results at 0.042 (p

The Effect Of Hyaluronic Acid To Pain Intensity Rated By Face Pain Scale-Revised On Knee Osteoarthritis Patients Hartono, Felicia Putri; Nugroho, Nunung; Isbandiati, Endang
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 1 (2020): January
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Introduction: Osteoarthritis (OA), is a chronic degenerative disease of joint associated with cartilage and joints. The most common symptoms of OA patients is joint pain that worsen in activity and relieved with rest. Hyaluronic acid (HA) injection is one of the treatment that used to relieved joint pain in OA patients. Aim : The purpose of this study was to determine the effect of HA injection to pain intensity on knee OA degree 2 and 3 patients in PHC Hospital Surabaya. Methods : Analytic research study design with retrospective cross-sectional study with purposive sampling technique. The respondents in this study were patients with knee OA degree 2 and 3 that had been failed with conservative therapeutics within 3 until 6 months and had been injected with HA in PHC Hospital in Surabaya. The number of respondents that have been collected that fulfill the inclusion and exclusion criteria were 46 respondents. Results : based on data analysis with Wilcoxon Signed Ranks Test there was an effect of HA injection in pain reduction on knee OA degree 2 and 3 patients (p = 0,00001). Conclusion : There is an effect of HA injection in pain reduction on knee OA degree 2 and 3 patients.

ANTIBACTERIAL EFFECT OF GNETUM GNEMON L. LEAVES EXTRACT ON STAPHYLOCOCCUS AUREUS Dayoh, Paul J; Isbandiati, Endang; Rahayu, Titien
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 2 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i2.3187

Introduction: Bacterial infections are common among doctors in Indonesia, leading toincreased use of antibiotics. Increased use of antibiotics, if not accompanied by rationalindication, can increase bacterial resistance. At present, alternative medicine from naturalingredients is widely studied, one of which is the leaves of melinjo (Gnetum gnemon L.).Melinjo leaves are thought to contain antibacterial compounds such as flavonoids, tannins,saponins, and alkaloids. Staphylococcus aureus is a round Gram-positive bacteria, that formsÂ grape-like clusters. Staphylococcus aureus is a normal flora on the skin and human mucosaÂ but can be opportunistic pathogens that cause mild to severe infections such as sepsis.Â Pathogenic Staphylococcus aureus can produce hemolysis blood, frozen plasma, and produceÂ various extracellular enzymes and toxins. Purpose: This research aims to study the MIC and MBC of melinjo leaves extract on thegrowth of Staphylococcus aureus bacteria. This research is an experimental study with a posttestÂ controlÂ groupÂ design. Method: Testing conducted using the microdilution method. Melinjo leaves used were extracted by maceration method using 96% ethanol. Samples were Staphylococcus aureus ATCC 25923 from BBLK Surabaya.Results: extract of melinjo leaves (Gnetum gnemon L.) had MIC on Staphylococcus aureus bacteria at a concentration of 640 mg/ml. MBC was not obtained because the extract of melinjo leaves clotted above 1280 mg/ml concentration. Conclusion: Melinjo leaves extract has an antibacterial effect against Staphylococcus aureus, the MIC is obtained at a concentration of 640 mg/ml, and the MBC cannot be determined.

Effectiveness Between Mango Honey and Kesambi Honey As Antibacterial Against Streptococcus Pyogenes Borromeu, Salvador BHWD; Isbandiati, Endang; Pramono, Adi
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 3 (2020): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v2i3.2663

Introduction: Infectious disease is one of the biggest health problems in developing countries, including Indonesia. The development of the discovery of antibiotics was not balanced with the development of resistance that occurred after that Group A streptococcus Î² hemolytic causes various events of a severe degree of infection. Now many people use natural ingredients as an alternative to antibiotics, one of which is honey. Aim: Knowing the benefits of mango honey and kesambi honey as an antibacterial against Streptococcus pyogenes and knowing the difference in the effectiveness and potency to inhibit growth and kill Streptococcus pyogenes between the two honey. Method: Using the macro dilution method in determining the value of MIC and the streaking method to determine the value of MBC. Results: MIC mango honey at 15.625 ÂµL, MIC kesambi honey at 15.625 ÂµL, MBC mango honey at 15.625 ÂµL, and MBC kesambi honey at 31.25 ÂµL. Conclusion: There is a difference of MBC between mango honey and kesambi honey against Streptococcus pyogenes where mango honey at 15.625 ÂµL and kesambi honey at 31.25 ÂµL, there is no difference in MIC between mango honey and kesambi honey, and the effectiveness and potency of mango honey as an antibacterial against Sp higher than kesambi honey which was observed through differences in the value of MBC.

ANTIBACTERIAL EFFECT OF EUGENIA POLYANTHA WIGHT WITH CORYNEBACTERIUM DIPHTHERIAE NON-TOXIGENIC Murbarani, Ivita T; Isbandiati, Endang; Puspitasari, Chrisdina
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 2 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i2.3182

Introduction: Corynebacterium diphtheriae is a bacteria that causes diphtheria. In recentyears, the bacterium is resistant to antibiotics. Eugenia polyantha wight might be a suitableantibiotic alternative as it has antibacterial effect against Staphylococcus aureus. The contentof the antibacterial compound in Eugenia polyantha wight at specific concentrations isexpected to be able to kill the Corynebacterium diphtheriae.Purpose: This study aimed to examine the antibacterial effect of Eugenia polyantha wight toCorynebacterium diphtheriae non-toxic.Method: Bay leaf extract (Eugenia polyantha wight) made with maceration method. Thisresearch is an experimental study conducted in vitro using a microdilution test on 96 wellplates then count value with the streaking method to find out MBC.Results: bactericidal power was quite effective at the concentration of 40%, while at the concentrationof 80%-100% has very strong bactericidal power that could destroy the growth of bacteria 99.9%. Thehigher the concentration of bay leaves extract, the higher the bactericidal power of bacteria Conclusion: Eugenia polyantha wight has an antibacterial effect against Corynebacteriumdiphtheriae non-toxic.

ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF KITOLOD (Hippobroma longiflora) LEAVES AGAINST STREPTOCOCCUS PYOGENES Tanaja, Grace Isabella; Isbandiati, Endang; Wattimena, Inge
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 4, No 1 (2022): January
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v4i1.3578

Background: Antimicrobial resistance to antibiotics has been emerging as a serious issue in healthcare, hence the need to discover and develop new treatment alternatives. Chemical compunds found in medicinal plants can potentially be used to synthesize new antibiotic agents, one of which is the leaves of kitolod (Hippobroma longiflora). Phytochemical screening of kitolod leaves showed positive results for secondary metabolites presumed to have antibacterial effects. Objectives: The purpose of this study was to examine the antibacterial activity of ethanolic extract of kitolod leaves against Streptococcus pyogenes. Methods: Antibacterial activity of the ethanolic extract of various concentrations (0,25%, 1,75%, 3,25%, 4,75%, 6,25%) were evaluated by broth microdilution method on a 96-well microplate and by streaking on agar plates. Results: Minimium Bactericidal Concentration (MBC) of ethanolic extract of kitolod leaves against Streptococcus pyogenes was 1,75%. Minimum Inhibitory Concentration (MIC) of ethanolic extract of kitolod leaves against Streptococcus pyogenes could not be inferred. Conclusion: Ethanolic extract of kitolod leaves showed antibacterial activities against Streptococcus pyogenes.

ANTIBACTERIAL ACTIVITIES TEST OF RED GINGER (Zingiber officinale var. rubrum) ETHANOL EXTRACT ON STAPHYLOCOCCUS EPIDERMIDIS Budianto, Andrea; Dinata, Mulya; Isbandiati, Endang
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 4, No 2 (2022): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v4i2.3791

Background: Antibiotic is a drug often used against infections caused by bacteria; however, irrational use of antibiotics can cause multidrug resistance (MDR). Staphylococcus epidermidis is a bacteria that has developed an MDR characteristic against antibiotics. Staphylococcus epidermidis is a normal opportunistic flora. Said bacteria can produce a biofilm that enables the bacteria to adhere to prosthetic devices and enter the bloodstream. Red ginger (Zingiber officinale var. rubrum) is a medicinal plant considered antibacterial for its secondary metabolites.Purpose: This study aims to determine the antibacterial effect of red ginger (Zingiber officinale var. rubrum) ethanol extract against Streptococcus epidermidis using a non-equivalent control group design study.Method: Red ginger (Zingiber officinale var. rubrum) ethanol extract was made with the maceration method. This study uses a microdilution test on 96 well plates. The absorbance value was read using a spectrophotometer to determine the Minimum Inhibitory Concentration (MIC) value, and streaking agar was used to find the Minimum Bactericidal Concentration (MBC) value.Result: The result shows that the red ginger (Zingiber officinale var. rubrum) ethanol extract at a concentration of 62,5 Î¼g/mL inhibits 75% of bacteria growth, at 125 Î¼g/mL inhibits 60% of bacteria growth, at 250 Î¼g/mL inhibits 45% of bacteria growth, at 500Î¼g/mL inhibits 65% of bacteria growth and at 1.000Î¼g/mL inhibits 50% of bacteria growth. The streaking agar test result shows bacteria growth on every concentration.Conclusion: Red ginger (Zingiber officinale var. rubrum) ethanol extract in the concentration range of 62,5-1.000 Î¼g/mL have no MIC and MBC value against Staphylococcus epidermidis.Â

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