Rahayu, Titien
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Antibacterial Effect Of Muntingia Calabura L. Leaf Extract To Pseudomonas Aeruginosa Anggono, Yovita; Jong, F.X. Himawan H.; Rahayu, Titien
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 4 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.803 KB) | DOI: 10.33508/jwmj.v1i4.2250

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen which can cause infection in every part of the human body lacking normal defense. This bacteria is one of the leading cause of nosocomial infection in Indonesia. Treating infection caused by Pseudomonas aeruginosa is now more challenging due to its resistance to many classes of antibiotics. On the other hand, Muntingia calabura L. is a genus of plants in the family Elaeocarpaceae, which is spread widely throughout South East Asia, including Indonesia. Some of the researches in the past show antibacterial properties of these plants. Aim: to determine the antibacterial effect of Muntingia calabura L. Leaf extract to Pseudomonas aeruginosa. Method: This research is an experimental study using in vitro technique and non-equivalent control group design. The method used in this study was microdilution in 96-well microplate and concentrations of Muntinga calabura L. Leaf extract used in this study were 50-800mg/mL. Minimum Inhibitory Concentration (MIC) was measured by a spectrophotometer, while Minimum Bactericidal Concentration (MBC) was observed by direct streaking to the agar plate. Result: MIC value was found at the concentration range of 400-800 mg/mL, while MBC was found at concentration 800 mg/mL. Conclusion: There is a potential bactericidal effect of Muntingia calabura L. To Pseudomonas aeruginosa
The Difference In Neutrophil/Lymphocyte Ratio In Patients With Acute Appendicitis Without And With Perforation Wismaya, IGP Wegen; Tahalele, Paul L; Rahayu, Titien
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 4 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.455 KB) | DOI: 10.33508/jwmj.v1i4.2251

Abstract

Introduction: Acute appendicitis is an inflammation of the appendix vermiformis and is the most frequent cause of acute abdominal. Inflammation occurs due to microorganisms infection that enter the submucosal layer of the appendix and eventually involves all layers of the appendix wall. Acute inflammation can lead to an appendix lumen obstruction, resulting in venous blood engorgement and arterial stenosis. One of the acute appendicitis physiological response from the immune system is an increasing of neutrophils and decreasing of lymphocytes. The result of increasing neutrophils and decreasing lymphocyte are increasing the absolute ratio between neutrophils and lymphocytes Aim: To know the difference of neutrophil / lymphocyte ratio in patients with acute appendicitis without perforation and with perforation at Dr. M. Soewandhie District General Hospital Surabaya. Methods: This research is an analytical research with cross sectional study, with paired categorical analysis sampling method. Respondent of this research is 62 patients, which consist of 31 patients acute appendicitis without perforation and 31 patients acute appendicitis with perforation that fulfilled inclusion and exclusion criteria in January 2016-August 2017. This research was conducted at Dr. M. Soewandhie District General Hospital Surabaya from Saturday, August 19th, 2017 until Saturday, September 30th, 2017. Results: the difference of neutrophil / lymphocyte ratio in acute appendicitis patient without perofration and with perforation analyze by using Fisher?s Exact Test comparative statistic test, p value 0.000
ANTIBACTERIAL EFFECT OF GNETUM GNEMON L. LEAVES EXTRACT ON STAPHYLOCOCCUS AUREUS Dayoh, Paul J; Isbandiati, Endang; Rahayu, Titien
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 3, No 2 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v3i2.3187

Abstract

Introduction: Bacterial infections are common among doctors in Indonesia, leading toincreased use of antibiotics. Increased use of antibiotics, if not accompanied by rationalindication, can increase bacterial resistance. At present, alternative medicine from naturalingredients is widely studied, one of which is the leaves of melinjo (Gnetum gnemon L.).Melinjo leaves are thought to contain antibacterial compounds such as flavonoids, tannins,saponins, and alkaloids. Staphylococcus aureus is a round Gram-positive bacteria, that forms grape-like clusters. Staphylococcus aureus is a normal flora on the skin and human mucosa but can be opportunistic pathogens that cause mild to severe infections such as sepsis. Pathogenic Staphylococcus aureus can produce hemolysis blood, frozen plasma, and produce various extracellular enzymes and toxins. Purpose: This research aims to study the MIC and MBC of melinjo leaves extract on thegrowth of Staphylococcus aureus bacteria. This research is an experimental study with a posttest control group design. Method: Testing conducted using the microdilution method. Melinjo leaves used were extracted by maceration method using 96% ethanol. Samples were Staphylococcus aureus ATCC 25923 from BBLK Surabaya.Results: extract of melinjo leaves (Gnetum gnemon L.) had MIC on Staphylococcus aureus bacteria at a concentration of 640 mg/ml. MBC was not obtained because the extract of melinjo leaves clotted above 1280 mg/ml concentration. Conclusion: Melinjo leaves extract has an antibacterial effect against Staphylococcus aureus, the MIC is obtained at a concentration of 640 mg/ml, and the MBC cannot be determined.
ANTIBACTERIAL TEST OF SQUEEZED WATER OF KATUK LEAF ON STREPTOCOCCUS PYOGENES Novitayanti, Ni Putu S; Rahayu, Titien; Sindrawati, Sindrawati
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 4, No 1 (2022): January
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v4i1.3576

Abstract

Background: Many infectious diseases still can be found in developing countries, including Indonesia. The common type of infection is upper respiratory tract infection (URTI) in which one of the diseases is pharyngitis. About 5% - 40% of pharyngitis cases are caused by Streptococcus pyogenes. One of the treatments for bacterial infection is antibiotics, but the increasing number of antibiotic resistance causes the need to find other alternatives such as herbal plants for the treatment. The community of Bali, particularly in Singaraja, often consume the squeezed water of Katuk leaf (Sauropus androgynus (L.) Merr) to treat pharyngitis.Objectives: This study aims to determine the antibacterial effect of squeezed water of Katuk leaf on Streptococcus pyogenes by measuring the value of the minimum inhibitory concentration (MIC) and the value of the minimum bactericidal concentration (MBC).Methods: The squeezed water of Katuk leaf was obtained through the squeezing method. The antibacterial activity test used the microdilution method of 96 well plates, then the absorbance value was read using a spectrophotometer to determine the MIC value, and streaking was performed to the blood agar strengthened by the results of the spectrophotometric readings to determine the value of MBC.Results: The results showed that the 300 grams of squeezed water of Katuk leaf had MIC values at a concentration of 10% and MBC values at a concentration of 20%.Conclusion: It can be concluded that the squeezed water of Katuk leaf has an antibacterial effect on Streptococcus pyogenes.Keywords: Antibacterial, Sauropus androgynus (L.) Merr, Streptococcus pyogenes