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All Journal Jurnal Ilmiah Manuntung JFIOnline Indonesian Journal of Pharmaceutical and Clinical Research
Patilaya, Popi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Aktivitas Antelmintik Ekstrak Air Daun Puguntano Curanga fel-terrae (Lour.) Merr. Patilaya, Popi; Husori, Dadang Irfan
Jurnal Farmasi Indonesia Vol 7, No 4 (2015)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i4.195

Abstract

Penelitian ini dilakukan untuk menentukan aktivitas antelmintik ekstrak air daun puguntano (Curanga fel-terrae). Daun puguntano diekstraksi dalam air suling dengan pendidihan selama 10 menit. Cacing Pheretima posthuma dipaparkan ke dalam ekstrak air daun puguntano konsentrasi 10, 20, 30, dan 40 mg/ml selama 5 jam. Larutan albendazol 10 mg/ml dan salin masing-masing digunakan sebagai kontrol positif dan kontrol negatif. Aktivitas antelmintik ditentukan dengan mengamati waktu paralisis dan kematian Pheretima Posthuma. Hasil penelitian menunjukkan bahwa ekstrak air daun puguntano konsentrasi 20 - 40 mg/ml menyebabkan paralisis dan kematian Pheretima Posthuma masing-masing antara 14,27 - 36,82 menit dan 18,69 - 42,47 menit. Penelitian ini menunjukkan bahwa ekstrak air daun puguntano memiliki potensi aktivitas antelmintik.
KARAKTERISTIKEKSTRAK AIR DAUN PUGUNTANO [Curanga fel-terrae (Lour.) Merr.] YANG BERPOTENSI SEBAGAI ANTELMINTIK Patilaya, Popi; Husori, Dadang Irfan
Jurnal Ilmiah Manuntung Vol 1 No 1 (2015): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.106 KB) | DOI: 10.51352/jim.v1i1.18

Abstract

OBJECTIVE: This study aimed to determine the characteristics of the water extract of leaves puguntano (Curanga fel-terrae (Lour.) Merr.) Using spectroscopic methods and phytochemical screening.METHODOLOGY: phytochemical screening performed to analyze compounds alkaloids, flavonoids, glycosides, anthraquinone glycosides, saponins, tannins, cyanogenic glycosides, and triterpenoids / steroids. Analyses were performed using FTIR spectrophotometer (Shimadzu) with IR Solution software. The wavelength is set at 4000 – 400 cm-1 with a resolution of 4 cm-1 and 16 scanner.RESULTS: Puguntano leaf water extract contains flavonoids, glycosides, saponins, tannins, steroids, and terpenoids. Infrared spectrum of puguntano leaf aqueous extract showed a O – H fuctional group at 3313.71 cm-1, C – H at 2974.23 and 2881.65 cm-1, C = C at 1689.65 and 1597.06 cm-1, C – O at 1265.30 and 1076.28 cm-1 and group C – H aromatics at 813.96 cm-1 in the fingerprint region
EFFECT OF CASSAVA TUBER PROCESSING ON Candida albicans GROWTH ON MANIHOT DEXTROSE AGAR Patilaya, Popi; Sumantri, Imam Bagus; Keliat, Jane Melita
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 8 No. 01 (2025): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v8i01.20763

Abstract

This study evaluated the effect of cassava tuber processing on Candida albicans growth on Manihot Dextrose Agar (MDA). The sliced cassava tubers were divided into three groups. G1 was milled and dried in an oven at 60°C. G2 was directly dried in the oven. G3 was milled without drying. Each group was soaked in distilled water (1,000 mL) for 15 min and filtered with a flannel cloth. The filtrate was oven-dried, then pulverized to obtain the cassava powder. The powder (8 g) was combined with dextrose (20 g) and agar (15 g). The mixture was suspended in distilled water (1,000 mL), boiled, and sterilized at 121°C for 15 min. The solution was placed into a petri dish and allowed to form agar media. The media derived from G1, G2, and G3 were considered as F1, F2, and F3. A suspension of C. albicans was cultured on the media and incubated for 48 h. The fungal growth was observed by calculating the viable colonies. The data were analyzed with one-way ANOVA and followed by Duncan’s test at the confidence level of 95%. The results showed that C. albicans grown on media F1, F2, and F3 had colony numbers of 128±2.08 x 105 cfu/mL, 64±5.57 x 105 cfu/mL, and 44±3.51 x 105 cfu/mL, respectively. Statistical analysis indicated a significant difference in the fungal growth on the three media (p < 0.05). This study proved that the processing methods of cassava tubers for MDA significantly affected the growth of C. albicans.
Co-Authors Husori, Dadang Irfan Keliat, Jane Melita Sumantri, Imam Bagus