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All Journal Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Pharmaceutical and Clinical Research IJOEP : International Journal of Ecophysiology
Keliat, Jane Melita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Antibacterial Activity of Ethanol Extract of Red Spinach Leaves (Amaranthus tricolor L.) Extracted Using Microwave-Assisted Extraction Against Pseudomonas aeruginosa and Streptococcus pyogenes salim, Emil; Siregar, Syifa Sabila Yassarah; Keliat, Jane Melita
International Journal of Ecophysiology Vol. 6 No. 2 (2024): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v6i2.17356

Abstract

Background: Humans are among the most susceptible living beings to bacterial infections. Infectious wounds on the skin surface are easily colonized by various kinds of bacteria such as Pseudomonas aeruginosa and Streptococcus pyogenes. Red spinach (Amaranthus tricolor L.) contains flavonoids with antimicrobial properties. Objective: The aim of this study was to determine the antibacterial activity of ethanol extract of red spinach leaves (Amaranthus tricolor L.) against Pseudomonas aeruginosa and Streptococcus pyogenes. Methods: This research includes the preparation, characterization, phytochemical screening of dried powder and extract. Extraction was performed by microwave-assisted extraction (MAE) method using 70% and 96% ethanol variations and then antibacterial activity of ethanol extract of red spinach leaves against Pseudomonas aeruginosa and Streptococcus pyogenes bacteria was carried out by paper disc diffusion method (Kirby-Bauer test) to obtain the diameter of inhibition. Results: The 70% ethanol extract of red spinach leaves has the lowest concentrations of 0.78 mg/ml and 25 mg/ml against Pseudomonas aeruginosa and Streptococcus pyogenes bacteria with diameters of 7.23 mm, and 7.90 mm respectively. While the 96% ethanol extract of red spinach leaves has the lowest concentration of 0.78 mg/ml against Pseudomonas aeruginosa and Streptococcus pyogenes bacteria with a same diameter of 7.43 mm. Conclusion: Ethanol extract of red spinach leaves has antibacterial activity against Pseudomonas aeruginosa and Streptococcus pyogenes.
ANTIFUNGAL OF SOKA FLOWER (Ixora Chinensis Lam.) ETHANOL EXTRACT AGAINST Candida albicans AND Pityrosporum ovale Keliat, Jane Melita; Sumayyah, Tengku
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 8 No. 01 (2025): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v8i01.19926

Abstract

Pathogenic fungi are microorganisms that cause infectious diseases in humans and are capable of growing and developing in various environments. The types of fungi that most often cause skin infections are Candida albicans and Pityrosporum ovale. The soka flower plant (Ixora chinensis Lam.), commonly found in Indonesia, is known to have antibacterial properties. The antifungal strength of the soka flower comes from the natural chemicals in its ethanol extract, which stops the growth of Candida albicans and Pityrosporum ovale. The research method was conducted experimentally, which included stages such as the preparation of dry powder, characterization of dry powder, phytochemical screening both on dry powder and the ethanol extract of soka flowers, preparation of the ethanol extract, standardization of the extract, and evaluation of the antifungal activity of the ethanol extract against both types of fungi. The research results indicate that the minimum inhibitory concentration of the ethanol extract of the soka flower against Candida albicans is 5.5 mg/ml with an inhibition zone diameter of 8.8 mm ±0.05, while for Pityrosporum ovale, the minimum inhibitory concentration is 50 mg/ml with an inhibition zone diameter of 10.7 mm ±0.50. The statistical analysis from Kruskal-Wallis tests show a significant difference in the amounts of ethanol extract from soka flowers based on the size of the inhibition zones for Candida albicans and Pityrosporum ovale, with probabilities of 0.000 and 0.001, both of which are below 0.05.
EFFECT OF CASSAVA TUBER PROCESSING ON Candida albicans GROWTH ON MANIHOT DEXTROSE AGAR Patilaya, Popi; Sumantri, Imam Bagus; Keliat, Jane Melita
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 8 No. 01 (2025): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v8i01.20763

Abstract

This study evaluated the effect of cassava tuber processing on Candida albicans growth on Manihot Dextrose Agar (MDA). The sliced cassava tubers were divided into three groups. G1 was milled and dried in an oven at 60°C. G2 was directly dried in the oven. G3 was milled without drying. Each group was soaked in distilled water (1,000 mL) for 15 min and filtered with a flannel cloth. The filtrate was oven-dried, then pulverized to obtain the cassava powder. The powder (8 g) was combined with dextrose (20 g) and agar (15 g). The mixture was suspended in distilled water (1,000 mL), boiled, and sterilized at 121°C for 15 min. The solution was placed into a petri dish and allowed to form agar media. The media derived from G1, G2, and G3 were considered as F1, F2, and F3. A suspension of C. albicans was cultured on the media and incubated for 48 h. The fungal growth was observed by calculating the viable colonies. The data were analyzed with one-way ANOVA and followed by Duncan’s test at the confidence level of 95%. The results showed that C. albicans grown on media F1, F2, and F3 had colony numbers of 128±2.08 x 105 cfu/mL, 64±5.57 x 105 cfu/mL, and 44±3.51 x 105 cfu/mL, respectively. Statistical analysis indicated a significant difference in the fungal growth on the three media (p < 0.05). This study proved that the processing methods of cassava tubers for MDA significantly affected the growth of C. albicans.
Training on Herbal Soap Preparation From the Extract Combination of Ketepeng Leaf and Sirih Leaf Nugraha, Sony Eka; Nainggolan, Marline; Keliat, Jane Melita; Maha, Hetty Lendora
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 1 (2022): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1136.678 KB) | DOI: 10.32734/abdimastalenta.v7i1.7222

Abstract

The problem of sanitation and hygiene is one of the problems faced by many people in Indonesia, especially in the current state of the Covid 19 pandemic. Not to mention the thing that is faced by the people of the Mabar village, Medan Deli sub-district, Medan City is the poor sanitation and hygiene of the community, especially those who live in dense settlements, so it is necessary to campaign for healthy living behaviors, one of which is through counseling on healthy living behaviors in maintaining personal health during the COVID-19 pandemic. and accompanied by training in the manufacture of antibacterial soap to increase public interest and awareness regarding the importance of healthy living. Soap products have developed into a primary need in today's society. These products are used every day by all levels of society, both upper, middle, and lower classes. The partners involved in community service are community groups in Neighborhood IX, Mabar sub-district, Medan Deli sub-district, Medan City. which is quite potential to be developed through a training program for making antibacterial herbal soaps, one of which is made from ketepeng leaves and betel leaves which are widely believed by the community to be effective in curing skin diseases. Several studies have proven that ketepeng and betel leaves have high antibacterial activity which have the potential to be developed into antibacterial soap products.Housewives and teenagers of productive age who are the targets of this service do not yet have productive activities. This training program is expected to provide business opportunities to the community so that it can improve the economy. The soap products produced can be used to meet household needs or can be sold to the surrounding community. With training in making antibacterial herbal soaps, expenses can be reduced and even can improve the household economy.
Training In Making Supplementary Foods and Stunting Prevention Education Through Android-Based “Anak Sehat” Digital Application at Kelurahan Petapahan, Lubuk Pakam Sub-District, Deli Serdang Regency Eka Nugraha, Sony; Marianne; Keliat, Jane Melita
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i1.10346

Abstract

Teaching, carrying out research for the development of science and applying knowledge, all three of which form a single unit known as the Tridharma of Higher Education. Community service is the third dharma of the Tridharma of Higher Education. The existence of community service is actually very important both for the management of the tertiary institution as well as for the existence of the tertiary institution in the community. The community service carried out is "Training on Making Complementary Food for Breastfeeding and Stunting Prevention Counseling Through the Android-Based "Healthy Children" Digital Application in Petapahan Village, Lubuk Pakam District, Deli Serdang Regency" The target group is mothers who have babies aged 12 months to 5 years in Posyandu Working area Petapahan Village, Lubuk Pakam District. Toddlerhood is an important period in the process of human development. Development and growth at that time determines the success of the growth and development of children in the next period. The period of growth and development at this age is a period that takes place quickly and will never be repeated, because it is often called the golden age or the golden age. Therefore, with this PkM activity, it can increase mother's awareness to pay attention to child development and prevent stunting.
Evaluation of Mother's Knowledge about Stunting and Observation of Stunting Incidents Based on the Growth of Toddlers at Posyandu Anggrek in Petapahan Village, Lubuk Pakam District, Deli Serdang Regency Eka Nugraha, Sony; Marianne; Keliat, Jane Melita; Kumar, Manan; Febiola P, Dian; Togatorop, Grace Mutiara
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i1.11047

Abstract

This community service aims to provide education about stunting and its prevention in order to increase awareness of mothers so that they can help their children in a period of normal growth and development while reducing the incidence of stunting in Indonesia. This community service activity is carried out by providing counseling related to stunting prevention and filling out questionnaires, especially for mothers who have babies aged 12 months to 5 years at Posyandu Anggrek to determine the effect of this activity on the level of knowledge of mothers regarding stunting issues.The community service carried out is " Evaluation of Mother's Knowledge about Stunting and Observation of Stunting incidents based on the growth of Toddlers at Posyandu Anggrek in Petapahan Village, Lubuk Pakam District, Deli Serdang Regency" The target group is mothers who have babies aged 12 months to 5 years in Posyandu Working area Petapahan Village, Lubuk Pakam District. Toddlerhood is an important period in the process of human development. Development and growth at that time determines the success of the growth and development of children in the next period. The period of growth and development at this age is a period that takes place quickly and will never be repeated, because it is often called the golden age or the golden age. Therefore, with this PkM activity, it can increase mother's awareness to pay attention to child development and prevent stunting.
Co-Authors . Marianne Eka Nugraha, Sony Emil Salim Febiola P, Dian Kumar, Manan Maha, Hetty Lendora Marline Nainggolan Patilaya, Popi Siregar, Syifa Sabila Yassarah Sumantri, Imam Bagus Sumayyah, Tengku Togatorop, Grace Mutiara