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All Journal Journal of Mathematical and Fundamental Sciences INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Hydrogen: Jurnal Kependidikan Kimia Berkala Penelitian Hayati
Vanawati, Noviana
Biology Study Program, School Of Life Sciences And Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences

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Role of Hypoxia on Growth and Differentiation of Human Adipose Derived Stem Cells Grown on Silk Fibroin Scaffold Induced by Platelet Rich Plasma Anggraini Barlian; Marselina Irasonia Tan; Ergha Widya Sarjana; Noviana Vanawati
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 3 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.3.6

Abstract

Previous research has proven that 10% platelet-rich plasma (PRP) can enhance growth and differentiation of human adipose derived stem cells (hADSC) grown on silk fibroin scaffold into chondrocytes. A low oxygen concentration (hypoxia) condition is an important factor that potentially affects the ability of hADSC to grow and differentiate. The objective of this research was to analyze the difference in growth and differentiation capacity of hADSC grown on salt leached silk fibroin scaffold supplemented by 10% PRP under normoxic and hypoxic conditions. The growth capacity of the hADSC was determined by MTT assay and differentiation was tested using glycosaminoglycan (GAG) content analysis, while chondrocyte markers were visualized with the immunocytochemistry (ICC) method. This research observed hADSC proliferation under normoxic and hypoxic conditions for 21 days. Visualization of type 2 collagen showed that it was more abundant under hypoxia compared to normoxia.  HIF-1α was only detected in the hADSC cultured in hypoxic conditions. In conclusion, culture under hypoxic conditions increases the capacity of hADSC to grow and differentiate into chondrocytes. This is the first study that has shown that hypoxia is able to enhance the proliferation and differentiation of hADSC grown on 3D salt leached silk fibroin scaffold supplemented by 10% PRP.
THE PATTERNS OF SEX DETERMINATION AND DIFFERENTIATION GENES IN GREEN SEA TURTLE (Chelonia mydas) Anggraini Barlian; Noviana Vanawati; Fitria D. Ayuningtyas
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.808 KB) | DOI: 10.23869/104

Abstract

Green sea turtle (C. mydas) is one of TSD (Temperature-dependent Sex Determination, TSD) animals which mean that their sex is determined by the egg’s incubation temperature. Genotypic Sex Determination (GSD) homologous genes play a role in TSD process. Until now, research on the pattern of sex determination genes in C.mydas has not been conducted yet. The aim of this research is to reveal sex determination and differentiation genes expression in Mesonephros-Gonad (MG) complexes of C. mydas embryos which incubated in masculinizing temperature (MT) and feminizing temperature (FT). C. mydas eggs were incubated in 3 different stage of TSP (Thermosensitive Period) at masculinizing temperature (26±10C, MT) and feminizing temperature (31±10C FT). Mesonefros-gonad complexes were isolated at Pre-TSP stage (FT at 14th day, MT at 24th day), TSP stage (FT at 24th day, MT at 36th day) and differentiated stage (FT at 40th day, MT at 58th day). RNA from mesonephros-gonad (MG) complexes were converted into cDNA by RT-PCR process. Pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9 and Rspo1 genes expression were analyzed by quantitative Real Time PCR (qPCR) method with β-actin gene as an internal control. Result of this study shown that expression pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9, and Rspo1genes in gonadal embryo of C. mydas were increased during gonadal development stage. Four genes expression patterns (Wnt4, Fgf9, Rspo1, and FoxL2) have shown that these genes have role in sexual differentiation rather than in sexual determination.
Free-Radical Scavenging and In Vitro Anticancer Assay of Indonesian Black Garlic with Different Time Processing Riyadi, Sandra Amalia; Vanawati, Noviana; Farah, Harra Ismi; Hafizah, Aulia UI; Hasanah, Uswatun; Herlina, Eka
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 6 (2025): December 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i6.18919

Abstract

The process of producing Black Garlic (BG) in Indonesia has not used a standard method and the right dosage. BG is produced through termal processing, compared with fresh garlic (FG) there are obvious changes in color, taste, and biological activities. BG was produced by different time of heating. Several studies have shown that BG has several pharmacological activities including antioxidants and anticancer. The aim of this research is to determine the optimal produced of BG to assess the potential antioxidant and anticancer activity. FG was heated at high temperature 60-70ºC with 85-95% humidity without any additives for various period of time which is 30, 40, 50, 60 days. Ethanol (EtOH) extract of BG 30; 40; 50 and 60 days and FG as a control were partionate with n-hexane, ethyl acetate (EtOAc), n-buthanol (n-BuOH), and water then evaporated in 40 °C. Each extract of FG and BG were tested Free-radical scavenging and anticancer activity. Free-radical scavenging was determined by 1,1 diphenyl-2-picrylhydrazyl (DPPH) method, and cytotoxicity of crude extract was tested by 3-(4,5-dimethylthiazol-2-yl)—2,5-diphenyltetrazolium bromide (MTT) assay against MCF-7 human breast cancer cell line. The result demonstrated that BG exhibited significantly enhanced radical scavenging activity compared to FG across different aging periods. Notably, EtOAc and n-hexane fractions obtained after 30, 40, and 60 days of thermal aging showed strong antioxidant activity with IC₅₀ values below 50 µg/mL. Importantly, this study demonstrates that a defined thermal aging duration of 30 days, combined with EtOAc fractionation, yields the most potent antioxidant fraction and the lowest IC₅₀ value (47.27 µg/mL) against MCF-7 cells, indicating that thermal aging time is a key determinant governing the antioxidant and anticancer potency of BG. These findings provide novel evidence linking controlled thermal aging parameters to the optimization of BG bioactivity, thereby offering a robust scientific basis for standardized BG processing with enhanced functional and therapeutic potential.
COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH Fitri Fadhilah; Noviana Vanawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1075

Abstract

The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.
Co-Authors Anggraini Barlian Eka Herlina Ergha Widya Sarjana Farah, Harra Ismi Fitri Fadhilah Fitria D. Ayuningtyas Hafizah, Aulia UI Marselina Tan Sandra Amalia Riyadi Uswatun Hasanah