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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Hydrogen: Jurnal Kependidikan Kimia
Vanawati, Noviana
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Chemistry Education Health Professions Social Sciences

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Free-Radical Scavenging and In Vitro Anticancer Assay of Indonesian Black Garlic with Different Time Processing Riyadi, Sandra Amalia; Vanawati, Noviana; Farah, Harra Ismi; Hafizah, Aulia UI; Hasanah, Uswatun; Herlina, Eka
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 6 (2025): December 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i6.18919

Abstract

The process of producing Black Garlic (BG) in Indonesia has not used a standard method and the right dosage. BG is produced through termal processing, compared with fresh garlic (FG) there are obvious changes in color, taste, and biological activities. BG was produced by different time of heating. Several studies have shown that BG has several pharmacological activities including antioxidants and anticancer. The aim of this research is to determine the optimal produced of BG to assess the potential antioxidant and anticancer activity. FG was heated at high temperature 60-70ºC with 85-95% humidity without any additives for various period of time which is 30, 40, 50, 60 days. Ethanol (EtOH) extract of BG 30; 40; 50 and 60 days and FG as a control were partionate with n-hexane, ethyl acetate (EtOAc), n-buthanol (n-BuOH), and water then evaporated in 40 °C. Each extract of FG and BG were tested Free-radical scavenging and anticancer activity. Free-radical scavenging was determined by 1,1 diphenyl-2-picrylhydrazyl (DPPH) method, and cytotoxicity of crude extract was tested by 3-(4,5-dimethylthiazol-2-yl)—2,5-diphenyltetrazolium bromide (MTT) assay against MCF-7 human breast cancer cell line. The result demonstrated that BG exhibited significantly enhanced radical scavenging activity compared to FG across different aging periods. Notably, EtOAc and n-hexane fractions obtained after 30, 40, and 60 days of thermal aging showed strong antioxidant activity with IC₅₀ values below 50 µg/mL. Importantly, this study demonstrates that a defined thermal aging duration of 30 days, combined with EtOAc fractionation, yields the most potent antioxidant fraction and the lowest IC₅₀ value (47.27 µg/mL) against MCF-7 cells, indicating that thermal aging time is a key determinant governing the antioxidant and anticancer potency of BG. These findings provide novel evidence linking controlled thermal aging parameters to the optimization of BG bioactivity, thereby offering a robust scientific basis for standardized BG processing with enhanced functional and therapeutic potential.
COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH Fadhilah, Fitri; Vanawati, Noviana
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1075

Abstract

The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.
Co-Authors Eka Herlina Farah, Harra Ismi Fitri Fadhilah, Fitri Hafizah, Aulia UI Sandra Amalia Riyadi Uswatun Hasanah