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All Journal EduChemia: Jurnal Kimia dan Pendidikan Jurnal Ilmiah Arena Tekstil CHEMPUBLISH JOURNAL AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Jurnal Ilmiah Farmako Bahari Molekul: Jurnal Ilmiah Kimia
Sandra Amalia Riyadi
Sekolah Tinggi Analis Bakti Asih
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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APLIKASI EKSTRAK JINTAN HITAM (Nigella sativa L.) SEBAGAI ZAT ANTIBAKTERI PADA KAIN KAPAS DENGAN VARIASI METODE Leli Nur Rina Hidayat; Sandra Amalia Riyadi; Srie Gustiani; Anisa Dwicahya
Arena Tekstil Vol 37, No 1 (2022)
Publisher : Balai Besar Tekstil

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31266/at.v37i1.7730

Abstract

Salah satu bahan sandang yang banyak digunakan oleh masyarakat adalah kain kapas, yang terkenal dengan kenyamanannya. Sayangnya, kain kapas sangat rentan terhadap kolonisasi bakteri yang dapat menyebabkan masalah pada kulit. Oleh karena itu, dipandang perlu untuk menambahkan zat antibakteri pada kain kapas. Pada penelitian ini, zat antibakteri yang diaplikasikan pada kain kapas adalah ekstrak jintan hitam. Langkah awal yang dilakukan adalah menentukan konsentrasi optimum ekstrak jintan hitam dengan variasi 10%, 20%, 30%, 40%, dan 50% untuk diaplikasikan pada kain kapas menggunakan cara rendam peras-pemanasawetan (pad-dry-cure). Setelah  diperoleh konsentrasi optimum, ekstrak diaplikasikan pada kain kapas dengan beberapa variasi metode, yaitu (1) tanpa plasma lucutan korona dan asam sitrat, (2) dengan plasma lucutan korona saja, (3)  dengan asam sitrat saja, dan (4) dengan plasma lucutan korona dan asam sitrat. Pengujian kemampuan antibakteri ekstrak jintan hitam dilakukan menggunakan metode AATCC TM100-2019. Hasil penelitian menunjukkan bahwa ekstrak jintan hitam memiliki kemampuan sebagai zat antibakteri pada kain kapas. Kain kapas dengan ekstrak 30%, 40%, dan 50% menghasilkan persentase reduksi tertinggi, yakni berturut-turut 96,6%; 97,0%; dan 97,0% terhadap S. aureus dan 79,4%; 79,5%; dan 79,5% terhadap E. coli. Metode yang menghasilkan persentase reduksi tertinggi adalah penggabungan teknologi plasma lucutan korona dan asam sitrat, yang menghasilkan peningkatan persentase reduksi menjadi 99,9% terhadap S. aureus dan E. coli pada konsentrasi ekstrak 30%. Metode ini juga menghasilkan durabilitas (daya tahan) tertinggi terhadap pencucian rumah tangga. Setelah 9 kali pencucian rumah tangga, persentase reduksi hanya menurun dari 99,9±0,14% menjadi 77,7±0,3% terhadap S. aureus dan 99,9±0,12% turun menjadi 75,1±0,4% terhadap E. coli.
AKTIVITAS ANTIKANKER KURKUMINOID TERHADAP SEL MELANOMA B16-F10 Sandra Amalia Riyadi; Fajar Fauzi Abdullah; Fitri Fadhilah; Nurul Assidiqiah
Jurnal Ilmiah Farmako Bahari Vol 13, No 2 (2022): Jurnal Ilmiah Farmako Bahari
Publisher : Fakultas MIPA Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jfb.v13i2.1458

Abstract

Kurkuminoid berasal dari rimpang kunyit (Curcuma Longa L.) yang memiliki ketiga komponen utama penyusunnya diantaranya kurkumin, demetoksikurkumin dan bisdemetoksikurkumin. Secara struktur ketiganya hanya berbeda pada subtituen gugus metoksi, namun mampu memberikan efek biologis yang berbeda. Kurkumin dalam beberapa tahun kebelakang menjadi salah satu target pengembangan penelitian sebagai agen antikanker yang dilakukan terhadap beberapa sel kanker. Namun, kemampuan kurkumin sebagai antikanker menjadi sangat terbatas akibat rendahnya kelarutan kurkumin dalam air, yang mempengaruhi rendahnya penyerapan senyawa kurkumin oleh sel dalam tubuh. Maka dari itu menjadi sangat perlu dilakukannya penelitian lebih lanjut untuk melihat aktivitas antikanker dari senyawa kurkumin lainnya yaitu demetoksikurkumin dan bisdemetoksikurkumin. Rimpang kunyit yang telah dikeringkan kemudian dimaserasi dengan etanol selama 3x24 jam kemudian maserat yang diperoleh diuapkan dengan rotary evaporator sehingga didapatkan ekstrak pekat etanol kunyit. Selanjutnya dilakukan partisi dengan pelarut n-heksan, etil asetat, dan n-butanol. Fraksi etil asetat kemudian dipekatkan dan dilakukan berbagai jenis metode pemisahan sehingga didapatkan ketiga senyawa kurkuminoid. Selanjutnya, isolat yang sudah terkarakterisasi di lakukan uji sitotoksik terhadap sel B16-F10 dengan menggunakan Metode Reduksi Resazurin. Hasil penelitian menunjukkan adanya perbedaan aktivitas penghambatan yang signifikan diantara ketiga isolat. Bisdemetoksikurkumin memiliki aktivitas penghambatan tertinggi dengan nilai IC50 16,20 µg/mL. Setelah itu, demetoksikurkumin dengan nilai IC50 22,59 µg/mL dan yang terakhir adalah kurkumin dengan nilai IC50 152,71 µg/mL.
Effect of Molybdenum on Population of Bacillus Consortium and Production of Indole 3-Acetic Acid Aliya Zahrah Adawiah; Sandra Amalia Riyadi; Reginawanti Hindersah; Pujawati Suryatmana
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v12i1.1771

Abstract

Molybdenum (Mo) is one of nutrients that contributes on process of fixing N2 and it’s contained in growth medium of N2-fixing bacteria. Bacillus is bacterium that widely used as biofertilizer because it can fix N2 in the atmosphere and produce phytohormones in form of Indole 3-Acetic Acid (IAA) which are benefical for plant growth. The purpose of this research was to determine the effect of Mo addition on Bacillus consortium in a growth medium of 2%molasses + 10grams peptone + 3grams beef extract against vegetative cell population, spore population and IAA hormone production after incubated for 3, 6 and 9 days. This research was conducted at Soil Biology Laboratory, Faculty of Agriculture, Padjadjaran University. The method to calculate total population of vegetative cells is the serial dilution plate method with 80ºC heating for 30 minutes for total spore population, while the production of IAA hormone was measured using UV-Vis spectrophotometer with Salkowski reagent at a wavelength of 530 nm. All data subjected to Student-t test with p ≤ 0.05. The results of the study by using the Student-t test showed that Mo increased the vegetative cell population on day 3 but did not affect the vegetative cell population on day 6 and 9. The spore population of the Bacillus consortium was not determined by Mo. The production of IAA hormone in cultures with Mo was not different from that in cultures without Mo.
The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines Supratman, Unang; Kautsari, Arsi; Naini, Al Arofatus; Riyadi, Sandra Amalia; Mayanti, Tri; Harizon, Harizon; Fajriah, Sofa
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9406

Abstract

Sesquiterpenoids belong to a group of terpenoid compounds with interesting structures that are abundant in natural products especially in higher plants. Sesquiterpenoids have a wide variety of bioactivities with great potential cytotoxic activity. The species Dysoxylum excelsum belongs to the Meliaceae family known as higher plant, but only a few sesquiterpenoids have been reported particularly for their cytotoxic activity. Therefore, this research aims to isolate and elucidate the sesquiterpenoids structure from D. excelsum stem bark and examines their cytotoxicity against HeLa cervical cancer cells. Through various column chromatography separations, four known sesquiterpenes namely β-caryophyllene oxide (1), caryophyllenol II (2), humulene dioxide A (3), and guai-6-en-10β-ol (4) were acquired from the n-hexane extract. Compounds 1-4 were isolated for the first time from D. excelsum species. The sesquiterpenoid structures were elucidated according to Nuclear Magnetic Resonance, Infrared, and HR-TOF-MS analysis. The cytotoxicity compounds 1-4 was determined against HeLa cervical cancer cells by examination with the PrestoBlue method and compound 3 exhibited the most potent cytotoxicity with an IC50 value of 160.74 µM. Keywords: cytotoxic activity, Dysoxylum excelsum, HeLa, Meliaceae, sesquiterpenoid.
The Cytotoxic Evaluation of Steroids Isolated from Dysoxylum alliaceum (Blume) Blume ex A.Juss. Riyadi, Sandra Amalia; Naini, Al Arofatus; Mayanti, Tri; Lesmana, Ronny; Azmi, Mohamad Nurul; Supratman, Unang
Molekul Vol 19 No 3 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.3.11439

Abstract

Dysoxylum alliaceum belongs to the Dysoxylum genus (Meliaceae) and there are few reports concerning the phytochemical components of this plant. To examine the chemical constituents of Dysoxylum alliaceum stem bark, a phytochemical study has been conducted and identified five known steroids, 3β,16β-dihydroxy-24(S)-methyl cholestenol (1), ergosta-5,22-dien-3β-ol (2), ergosta-7,24(28)-dien-3β-ol (3), 22(E)-ergosta-6,22-dien-3β,5α,8α-triol (4), and 20α-dihydroprogesterone (5) from its ethanolic extract. Spectroscopic data such as FT-IR, HR-ESI-MS, 1D, and 2D NMR as well as comparison with previously published spectral data were used to identify the chemical structures of compounds 1–5. Furthermore, these steroids 1-5 were assessed in vitro regarding their cytotoxic effect against A549 lung cancer cell lines and revealed weak to inactive with IC50 values ranging from 68.52 to >150 µM. Keywords: A549 cell lines, cytotoxic evaluation, Dysoxylum alliaceum (blume) blume ex a.juss, meliaceae, steroid
Identification and Antibacterial Activity of Bioactive Compounds Extracted from Gading Coconut (Cocos nucifera var. eburnea) Leaf Hasanah, Uswatun; Mulyati, Ade Heri; Riyadi, Sandra Amalia; Pita, Lusia Magdalenis
EduChemia: Jurnal Kimia dan Pendidikan Vol 10, No 1 (2025)
Publisher : Department of Chemistr Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/educhemia.v10i1.31974

Abstract

Traditional medicine continues to play a vital role in Indonesia’s healthcare landscape, with increasing reliance on plant-derived remedies for disease prevention and treatment. Among these, the gading coconut (Cocos nucifera var. eburnea), native to Bogor, Indonesia, presents untapped potential as a source of antibacterial agents. This study aimed to identify bioactive compounds with antibacterial activity from gading coconut leaves and to evaluate the impact of extraction methods—maceration and sonication—on their yield and efficacy. Compared with maceration (5.92%), sonication produced a significantly greater extract yield (9.48%). Phytochemical screening confirmed the presence of alkaloids, flavonoids, and tannins in both extracts. Quantitative analysis revealed that the sonicated extract contained greater concentrations of total flavonoids (52.06 mg QE/g) and total phenolics (147.10 mg GAE/g). Chemical profiling using liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) identified key antibacterial constituents, including 3-hydroxycoumarin, isoorientin, vitexin, and α-linolenic acid. Biological evaluation demonstrated that the sonicated extract exhibited stronger antibacterial activity, with minimum inhibitory concentrations (MICs) of 50 mg/mL against Escherichia coli and 25 mg/mL against Staphylococcus aureus. These findings suggest that the extraction method significantly influences both the chemical composition and antibacterial potency of gading coconut leaf extracts. Overall, this study highlights the pharmacological relevance of C. nucifera var. eburnea as a promising source of natural antibacterial agents and contributes to the scientific validation of traditional medicinal plants in Indonesia.
Pewarnaan Preparat Apus Tipis Malaria (Plasmodium vivax Dan Plasmodium falcifarum) Menggunakan Ekstrak Murbei Sebagai Pengganti Eosin Pada Komposisi Giemsa Riyadi, Sandra Amalia; Fadhilah, Fitri
Chempublish Journal Vol. 6 No. 2 (2021): Chempublish Journal
Publisher : Department of Chemistry, Faculty of Science and Technology Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/chp.v6i2.22464

Abstract

Malaria is caused by the plasmodium parasite and still a threat to Indonesia. The main examination is staining of thick and thin smear preparations using Giemsa. Because giemsa is expensive, its waste is difficult to decompose, toxic and rare. Therefore, there is a need for new inventions to utilize the potential of natural components like mulberry fruit, which contains anthocyanins for coloring agents, to lessen the impact and risk of giemsa. The purpose of this study was to determine the effect of macerated ethanol extract of mulberry with a ratio of 1:3 (w/v) as an alternative to eosin in Giemsa on staining of thin blood smears of malaria, as well as knowing the best concentration in staining. This type of experimental research by observing the clarity of shape, color of parasites P.vivax, P.falcifarum, erythrocyte cells also compared with 3% giemsa as a control. This study consisted of 32 samples, 8 repetitions and 4 treatments namely P1 (3% Giemsa control), Giemsa modified mulberry eosin P2 (20%), P3 (30%), P4 (40%). The quality of the preparations was assessed based on the clarity and contrast of the colors. Kruskal-Wallis and Mann-Whitney data analysis techniques were used. The results showed that mulberry extract could be an alternative dye to replace eosin in the composition of giemsa on malaria thin smear preparations. The most effective concentration was 30% which could give the Plasmodium nuclei the same quality of staining as the control, while for the erythrocytes the staining quality was not the same as the control.
The Phenolic Compounds Isolated from Myristica fragrans and Their Cytotoxic Effects on B16-F10 Melanoma Cancer Cell Lines Hasbilla, Raihan Fathurrahman; Riyadi, Sandra Amalia; Safriansyah, Wahyu; Hidayat, Ace Tatang; Susianti, Susianti; Salam, Supriyanto; Lesmana, Ronny; Retnowati, Rurini; Supratman, Unang
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.16265

Abstract

Phenolic compounds are a major type of secondary metabolite found in plants. These compounds are synthesized through shikimic and phenylpropanoid pathways, resulting in the formation of numerous unique structures and bioactivities. In addition, a significant amount has been reported in nutmeg, an endemic plant of Indonesia, which has been widely used in traditional medicine. A previous study also revealed that ethyl acetate extract of the plant has notable cytotoxic effects against melanoma B16-F10. Therefore, the purpose of this study is to isolate and evaluate phenolic compounds in nutmeg for their potential to inhibit B16-F10 melanoma cancer cell growth. The seeds extract of nutmeg was separated by various chromatographic techniques to yield a total of five compounds, which were identified through spectroscopic analysis (HR-TOF-ESI-MS, IR, and NMR) as well as comparison with literature. The compounds 1-5 were identified as (+)-veraguensin (1), 3',4',5'-trimethoxycinnamyl alcohol (2), (+)-galbegin (3), (-)-polysphorin (4), and 7-methoxycoumarin (5). Cytotoxic effects were then assayed against B16-F10 melanoma cell lines using the Resazurin method. Furthermore, compound 1 displayed the highest cytotoxic activity, with an IC50 value of 112.71 µM.
Co-Authors Ace Tatang Hidayat Aliya Zahrah Adawiah Anisa Dwicahya Azmi, Mohamad Nurul Fajar Fauzi Abdullah Fitri Fadhilah Fitri Fadhilah, Fitri Harizon Harizon Hasanah, Uswatun Hasbilla, Raihan Fathurrahman Kautsari, Arsi Leli Nur Rina Hidayat Mulyati, Ade Heri Naini, Al Arofatus Nurul Assidiqiah Pita, Lusia Magdalenis Pujawati Suryatmana Reginawanti Hindersah Ronny Lesmana Rurini Retnowati Safriansyah, Wahyu Salam, Supriyanto Sofa Fajriah Srie Gustiani Susianti, Susianti Tri Mayanti Unang Supratman