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All Journal Current Research on Biosciences and Biotechnology Narra J Journal of Multidisciplinary Applied Natural Science
Nugrahapraja, Husna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Global Perspectives on Environmental Microbiome Research: Current Status and Future Directions Putri, Wahyu Aristyaning; Subiastuti, Aprilia Sufi; Wulandari, Cahyo; Rachman, Mifta Pratiwi; Sebastian, Alfino; Siregar, Abdul Rahman; Himawan, Tyas Ikhsan; Priyono, Dwi Sendi; Sofyana, Neng Tanty; Purwestri, Yekti Asih; Nugrahapraja, Husna; Wibowo, Anjar Tri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.266

Abstract

The environmental microbiome plays an important role in various ecosystems around the world, influencing nutrient cycling, disease dynamics and ecosystem stability. This bibliometric analysis provides a comprehensive overview of the current state and future directions of environmental microbiome research from a global perspective from 2009–2024. Through systematic examination of Scopus publications, 2154 documents were found. The results show a significant increase in the number of publications since 2017 and a peak in 2024. The most cited document was "The hidden world within plants: Ecological and evolutionary considerations for defining functioning of microbial endophytes" with 1887 citations and “Structure and function of the global ocean microbiome” with 1843 citations. The most productive countries in environmental microbiome research are the United States (n = 748), China (n = 533), India (n = 308), Germany (n = 172) and the United Kingdom (n = 157). Microbiome, microbial community, microfolora, microbiota, microbiology, bacteria, and bacterium are the most popular topics that will continue to develop in the future. Although the United States has published the highest total number of papers, more recent studies have predominantly been published from China, indicating shift in the centre of study from the United States to China. Insights gained from this analysis contribute to a deeper understanding of the environmental microbiome research landscape, guiding future research priorities and collaborations in the field.
Bioconversion of inorganic selenium to organic selenium in the black soldier fly (BSF) larvae Sihombing, Rolina Anna Erica; Niloperbowo, Wardono; Nugrahapraja, Husna
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/JAFFPU8L

Abstract

Selenium is one of the essential micronutrients needed to fulfil livestock nutrition, which can be found in inorganic and organic forms. Black Soldier Fly (BSF) larvae can potentially be used as a natural converter from inorganic selenium to organic selenium. However, the capacity and response of BSF larvae to convert selenium are still unknown. This study aims to determine the effect of inorganic selenium administration on BSF larvae. The research method was the determination of selenium concentration by UV-Vis spectrophotometry method based on the variation in the age of inorganic selenium administration in BSF larvae to the growth of BSF larvae, and the accumulation of selenium in BSF larvae, with variations in age of 0, 4, 8, and 12 days of age given sodium selenite (Na2SeO3) and control without administration of sodium selenite from the beginning to the end of rearing. There was no significant difference (p > 0.05) in the growth performance of BSF larvae and the accumulation of selenium in BSF larvae in the age variation experiment of BSF larvae when given sodium selenite. Based on the acquisition of larval mass, the growth rate of BSF larvae in the control treatment, with sodium selenite at 1 mg/kg, was 0.129, 0.093, 0.037, 0.156, and 0.128 mg/day at 0, 4, 8, and 12 days, respectively. These results indicate that inorganic selenium administration to BSF larvae can occur during the rearing period. In the experiment of variation in the concentration of inorganic selenium given to BSF larvae, the growth rate of BSF larvae was significantly higher (p < 0.05) when given 1000 mg/kg of sodium selenite, which was 0.467 mg/day.
Development of a novel multi-epitope peptide vaccine candidate against Mycobacterium tuberculosis using reverse vaccinology: A promising strategy for enhanced immunoprotection Hasan, Nur AHM.; Giri-Rachman, Ernawati A.; Nugrahapraja, Husna
Narra J Vol. 6 No. 1 (2026): April 2026
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v6i1.2897

Abstract

Tuberculosis (TB) is a leading cause of death worldwide, caused by Mycobacterium tuberculosis (Mtb). The existing Bacillus Calmette-Guerin (BCG) vaccine has limitations, especially its reduced effectiveness in adults. This research focuses on developing a multi-epitope Mtb vaccine candidate through reverse vaccinology, aiming for a more effective and widely applicable solution. The study used the Vaxign2 pipeline to identify Mtb antigenic proteins, including PPE35, mpt83, mrsA, and rplK. Cytotoxic T cells (CTL), helper T cells (HTL), and B-cells, were predicted and selected based on their antigenicity, non-allergenicity, and non-toxicity. The chosen epitopes from these proteins, 4 CTL, 1 HTL, and 1 B cell epitope, were assembled into a multi-epitope vaccine construct, incorporating the adjuvant PADRE and linkers (EAAAK, AAY, and GPGPG). The vaccine candidate has a molecular weight of 10.68 kDa, with stability, hydrophilicity, and solubility confirmed. Its 3D structure was validated for quality and accuracy. Docking and molecular dynamics simulations with immune receptors TLR2 and TLR4 showed strong, stable interactions. The global population coverage of the vaccine candidate was reaching 98.19%. In silico cloning into the pET30a(+) vector in Escherichia coli BL21(DE3) was successful, with codon optimization (CAI: 0.98) and a GC content of 54.6%. Immunity simulations indicated enhanced activation of antigen-presenting cells, CTL, HTL, B cells, and antibody production. Overall, this study suggests vaccine candidate is a promising multi-epitope vaccine candidate, warranting further in vivo testing, including protein expression in E. coli.
Co-Authors Abdul Rahman Siregar, Abdul Rahman Anjar Tri Wibowo Cahyo Wulandari Ernawati A. Giri-Rachman Hasan, Nur AHM. Himawan, Tyas Ikhsan Niloperbowo, Wardono Priyono, Dwi Sendi Putri, Wahyu Aristyaning Rachman, Mifta Pratiwi Sebastian, Alfino Sihombing, Rolina Anna Erica Sofyana, Neng Tanty Subiastuti, Aprilia Sufi Yekti Asih Purwestri