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All Journal Jurnal Perlindungan Tanaman Indonesia JMM (Jurnal Masyarakat Mandiri) Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Journal of Multidisciplinary Applied Natural Science
Subiastuti, Aprilia Sufi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Other

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Evaluation of Three Cucurbitaceae Cultivars Resistant against Begomoviruses Based on Morphological Symptoms and Molecular Analysis Subiastuti, Aprilia Sufi; Huda, Muhammad Syafi’atol; Daryono, Budi Setiadi
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.87915

Abstract

Cucurbitaceae is a group of annual horticultural crops with high economic prospects and benefits. The Faculty of Biology, Universitas Gadjah Mada has developed several new cultivars within the Cucurbitaceae family, such as the melon cultivar 'Hikapel', watermelon 'Citra Jingga', and butternut squash 'Citra Laga'. However, development of these cultivars has been hindered by yellow disease, typically triggered by Begomovirus infection. This study aimed to determine the morphological symptoms of yellow disease, to detect Begomovirus infection using molecular approach, and assess resistance levels of the three Cucurbitaceae cultivars. The method involved observing morphological symptoms in plants and measuring plant height. The degree of symptom severity was then converted into a vulnerability index (VI). The symptomatic leaf samples were tested using Krusty and Homer primer to confirm the symptoms caused by Begomovirus. The PCR results were sequenced and analyzed using Maximum Likelihood method in MEGA 11. The results showed that symptoms included curling tip of leaves, yellow mosaic on the leaves, and stunted growth in some plants, which were suggested as Begomovirus infection. Based on the Vulnerability Index (VI), the watermelon cultivar 'Hikapel' was considered susceptible to Begomovirus infection (VI = 85%), while the yellow squash 'Citra Laga' was moderately susceptible (VI = 57.5%). However, the watermelon 'Citra Jingga' was considered moderately resistant to this infection (VI = 50%). The presence of DNA the Begomovirus at three species Cucurbitaceae were characterized by a 550 bp band. The 'Hikapel' melon and 'Citra Laga' butternut were infected with Tomato leaf curl New Delhi virus (ToLCNDV), while the ‘Citra Jingga’ watermelon were infected with Squash leaf curl Philippine virus (SLCuPV).
Global Perspectives on Environmental Microbiome Research: Current Status and Future Directions Putri, Wahyu Aristyaning; Subiastuti, Aprilia Sufi; Wulandari, Cahyo; Rachman, Mifta Pratiwi; Sebastian, Alfino; Siregar, Abdul Rahman; Himawan, Tyas Ikhsan; Priyono, Dwi Sendi; Sofyana, Neng Tanty; Purwestri, Yekti Asih; Nugrahapraja, Husna; Wibowo, Anjar Tri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.266

Abstract

The environmental microbiome plays an important role in various ecosystems around the world, influencing nutrient cycling, disease dynamics and ecosystem stability. This bibliometric analysis provides a comprehensive overview of the current state and future directions of environmental microbiome research from a global perspective from 2009–2024. Through systematic examination of Scopus publications, 2154 documents were found. The results show a significant increase in the number of publications since 2017 and a peak in 2024. The most cited document was "The hidden world within plants: Ecological and evolutionary considerations for defining functioning of microbial endophytes" with 1887 citations and “Structure and function of the global ocean microbiome” with 1843 citations. The most productive countries in environmental microbiome research are the United States (n = 748), China (n = 533), India (n = 308), Germany (n = 172) and the United Kingdom (n = 157). Microbiome, microbial community, microfolora, microbiota, microbiology, bacteria, and bacterium are the most popular topics that will continue to develop in the future. Although the United States has published the highest total number of papers, more recent studies have predominantly been published from China, indicating shift in the centre of study from the United States to China. Insights gained from this analysis contribute to a deeper understanding of the environmental microbiome research landscape, guiding future research priorities and collaborations in the field.
The Potency of Organic Fertilizer in Reducing Leaf Curl Disease on Chili (Capsicum annuum L.): Potensi Pupuk Organik Dalam Mengurangi Penyakit Keriting Daun Pada Cabai (Capsicum annuum L.) Subiastuti, Aprilia Sufi; Windianingsih, Diah Fitri; Noviyani, Pipit; Kusnanda, Prima Sekti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.418

Abstract

Chili is an essential commodity in Indonesia, with demand continuing to rise; however, its production is often disrupted by leaf curl disease caused by viruses. One potential solution is using organic fertilizer enriched with beneficial microbes such as Plant Growth-Promoting Microorganisms (PGPM). Despite its potential, the role of PGPM in controlling viral diseases in chili plants has been rarely studied. This study aims to explore the effectiveness of organic fertilizer in reducing leaf curl infections by improving plant health. Two chili cultivars were used: 'Gama LBF' (a breeding product of PIAT UGM) and 'Ponirun' (a commercial variety known for its disease resistance). The chili plants were treated with various concentrations of organic fertilizer, and their effectiveness was evaluated based on disease incidence (the number of symptomatic plants) and disease severity (the level of visual symptoms), which were then converted into an index. Results showed that the 75% concentration yielded the lowest disease incidence in both cultivars—26.5% for 'Ponirun' and 42.85% for 'Gama LBF'. The lowest disease severity was also observed at the 75% concentration for 'Ponirun' and 50% for 'Gama LBF'. These findings highlight the importance of selecting the appropriate organic fertilizer concentration to suppress leaf curl disease in chili effectively.
TRANSFORMASI LIMBAH ORGANIK RUMAH TANGGA: PEMBUATAN PUPUK KOMPOS, POC, ECO-ENZYME DAN BIOPORI DI KABUPATEN GUNUNG KIDUL Pratiwi, Resti Diyah; Wisesa, Satria Adi; Dewanti, Indhi Restu; Oktariansiwi, Azizah Winardhita; Hardyta, Galy; Subiastuti, Aprilia Sufi
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 2 (2025): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i2.29283

Abstract

Abstrak: Ketidakhadiran tempat pembuangan akhir dan rendahnya pengetahuan masyarakat di Kalurahan Pilangrejo mengakibatkan limbah organik rumah tangga tidak dikelola dengan bijaksana. Permasalahan ini perlu ditangani dengan beberapa strategi seperti pengolahan limbah menjadi pupuk kompos, pupuk organik cair, eco-enzyme, dan lubang biopori. Tujuan pengabdian ini adalah untuk meningkatkan kesadaran dan pemahaman masyarakat terkait pentingnya pengolahan limbah organik rumah tangga sehingga menjadi produk bermanfaat. Sasaran kegiatan ini adalah anggota PKK dengan total peserta sejumlah 45 peserta dan dilaksanakan di dua lokasi yaitu Padukuhan Pilangrejo (sosialisasi eco-enzyme) dan di Padukuhan Wotgalih (pembuatan pupuk kompos, pupuk organik cair serta lubang biopori). Program ini terdiri dari tiga tahapan: survei dan perizinan, penyuluhan dan pelatihan, serta monitoring dan evaluasi melalui wawancara verbal dan kuisioner kepuasan. Hasil menunjukkan penigkatan pengetahuan terkait eco-enzyme hingga 97%. Sementara itu, penerapan teknologi biopori, pupuk organik cair (POC) serta pupuk kompos mendapatkan respons sangat setuju (9,09%), setuju (75%), dan cukup setuju (15,91%) terkait kebermanfaatan, ketertarikan dan pemahaman program pengabdian.Abstract: The absence of a final disposal site and the low level of public knowledge in Pilangrejo Village have resulted in household organic waste not being managed wisely. This problem needs to be addressed with several strategies such as processing waste into compost, making biopore holes, making liquid organic fertilizer and eco-enzyme. The purpose of this community service is to increase public awareness and understanding regarding the importance of processing household organic waste to facilitate its conversion into useful products. The target of this activity is PKK members with a total of 45 participants and the program is implemented in two locations, namely Pilangrejo Hamlet (eco-enzyme socialization) and Wotgalih Hamlet (socialization of making compost, liquid organic fertilizer, and bio pore holes). This program is structured in three stages: survey and licensing, counseling and training as well as monitoring and evaluation through verbal interviews and satisfaction questionnaires. The results show a 97% increase in knowledge about eco-enzyme. Meanwhile, the implementation of biopore, liquid organic fertilizer (POC), and compost received responses of strongly agree (9.09%), agree (75%), and moderately agree (15.91%) regarding usefulness, interest, and understanding of the program.
Co-Authors Abdul Rahman Siregar, Abdul Rahman Anjar Tri Wibowo BUDI SETIADI DARYONO Cahyo Wulandari Dewanti, Indhi Restu Hardyta, Galy Himawan, Tyas Ikhsan Huda, Muhammad Syafi’atol Kusnanda, Prima Sekti Noviyani, Pipit Nugrahapraja, Husna Oktariansiwi, Azizah Winardhita Pratiwi, Resti Diyah Priyono, Dwi Sendi Putri, Wahyu Aristyaning Rachman, Mifta Pratiwi Sebastian, Alfino Sofyana, Neng Tanty Windianingsih, Diah Fitri Wisesa, Satria Adi Yekti Asih Purwestri