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All Journal Jurnal Perlindungan Tanaman Indonesia Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Journal of Multidisciplinary Applied Natural Science
Subiastuti, Aprilia Sufi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Other

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Evaluation of Three Cucurbitaceae Cultivars Resistant against Begomoviruses Based on Morphological Symptoms and Molecular Analysis Subiastuti, Aprilia Sufi; Huda, Muhammad Syafi’atol; Daryono, Budi Setiadi
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.87915

Abstract

Cucurbitaceae is a group of annual horticultural crops with high economic prospects and benefits. The Faculty of Biology, Universitas Gadjah Mada has developed several new cultivars within the Cucurbitaceae family, such as the melon cultivar 'Hikapel', watermelon 'Citra Jingga', and butternut squash 'Citra Laga'. However, development of these cultivars has been hindered by yellow disease, typically triggered by Begomovirus infection. This study aimed to determine the morphological symptoms of yellow disease, to detect Begomovirus infection using molecular approach, and assess resistance levels of the three Cucurbitaceae cultivars. The method involved observing morphological symptoms in plants and measuring plant height. The degree of symptom severity was then converted into a vulnerability index (VI). The symptomatic leaf samples were tested using Krusty and Homer primer to confirm the symptoms caused by Begomovirus. The PCR results were sequenced and analyzed using Maximum Likelihood method in MEGA 11. The results showed that symptoms included curling tip of leaves, yellow mosaic on the leaves, and stunted growth in some plants, which were suggested as Begomovirus infection. Based on the Vulnerability Index (VI), the watermelon cultivar 'Hikapel' was considered susceptible to Begomovirus infection (VI = 85%), while the yellow squash 'Citra Laga' was moderately susceptible (VI = 57.5%). However, the watermelon 'Citra Jingga' was considered moderately resistant to this infection (VI = 50%). The presence of DNA the Begomovirus at three species Cucurbitaceae were characterized by a 550 bp band. The 'Hikapel' melon and 'Citra Laga' butternut were infected with Tomato leaf curl New Delhi virus (ToLCNDV), while the ‘Citra Jingga’ watermelon were infected with Squash leaf curl Philippine virus (SLCuPV).
Global Perspectives on Environmental Microbiome Research: Current Status and Future Directions Putri, Wahyu Aristyaning; Subiastuti, Aprilia Sufi; Wulandari, Cahyo; Rachman, Mifta Pratiwi; Sebastian, Alfino; Siregar, Abdul Rahman; Himawan, Tyas Ikhsan; Priyono, Dwi Sendi; Sofyana, Neng Tanty; Purwestri, Yekti Asih; Nugrahapraja, Husna; Wibowo, Anjar Tri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.266

Abstract

The environmental microbiome plays an important role in various ecosystems around the world, influencing nutrient cycling, disease dynamics and ecosystem stability. This bibliometric analysis provides a comprehensive overview of the current state and future directions of environmental microbiome research from a global perspective from 2009–2024. Through systematic examination of Scopus publications, 2154 documents were found. The results show a significant increase in the number of publications since 2017 and a peak in 2024. The most cited document was "The hidden world within plants: Ecological and evolutionary considerations for defining functioning of microbial endophytes" with 1887 citations and “Structure and function of the global ocean microbiome” with 1843 citations. The most productive countries in environmental microbiome research are the United States (n = 748), China (n = 533), India (n = 308), Germany (n = 172) and the United Kingdom (n = 157). Microbiome, microbial community, microfolora, microbiota, microbiology, bacteria, and bacterium are the most popular topics that will continue to develop in the future. Although the United States has published the highest total number of papers, more recent studies have predominantly been published from China, indicating shift in the centre of study from the United States to China. Insights gained from this analysis contribute to a deeper understanding of the environmental microbiome research landscape, guiding future research priorities and collaborations in the field.
The Potency of Organic Fertilizer in Reducing Leaf Curl Disease on Chili (Capsicum annuum L.): Potensi Pupuk Organik Dalam Mengurangi Penyakit Keriting Daun Pada Cabai (Capsicum annuum L.) Subiastuti, Aprilia Sufi; Windianingsih, Diah Fitri; Noviyani, Pipit; Kusnanda, Prima Sekti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.418

Abstract

Chili is an essential commodity in Indonesia, with demand continuing to rise; however, its production is often disrupted by leaf curl disease caused by viruses. One potential solution is using organic fertilizer enriched with beneficial microbes such as Plant Growth-Promoting Microorganisms (PGPM). Despite its potential, the role of PGPM in controlling viral diseases in chili plants has been rarely studied. This study aims to explore the effectiveness of organic fertilizer in reducing leaf curl infections by improving plant health. Two chili cultivars were used: 'Gama LBF' (a breeding product of PIAT UGM) and 'Ponirun' (a commercial variety known for its disease resistance). The chili plants were treated with various concentrations of organic fertilizer, and their effectiveness was evaluated based on disease incidence (the number of symptomatic plants) and disease severity (the level of visual symptoms), which were then converted into an index. Results showed that the 75% concentration yielded the lowest disease incidence in both cultivars—26.5% for 'Ponirun' and 42.85% for 'Gama LBF'. The lowest disease severity was also observed at the 75% concentration for 'Ponirun' and 50% for 'Gama LBF'. These findings highlight the importance of selecting the appropriate organic fertilizer concentration to suppress leaf curl disease in chili effectively.
Pemanfaatan pekarangan dan limbah rumah tangga untuk budidaya lele sebagai upaya memenuhi pangan keluarga selama pandemi covid-19 Subiastuti, Aprilia Sufi; Daryono, Budi Setiadi; Sukirno, Sukirno
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5 No 1 (2022)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i1.11323

Abstract

Pandemi Covid-19 menyebabkan perubahan kondisi sosial ekonomi masyarakat Indonesia. Sekitar 40% pekerja atau pelaku usaha mengalami perubahan pendapatan. Hal ini sedikit banyak mempengaruhi kemampuan masyarakat dalam memenuhi kebutuhan pangan keluarga. Pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada masyarakat untuk mengoptimalkan pemanfaatan pekarangan dan limbah rumah tangga untuk memenuhi kebutuhan pangan keluarga melalui budidaya lele. Pelaksanaan program dibagi ke dalam tiga tahap, yaitu pelatihan warga, pendampingan, dan evaluasi.Pelatihan warga meliputi pelatihan budidaya lele dan kangkung secara hidroponik menggunakan pot dan pelatihan pembuatan pakan lele dari limbah rumah tangga. Pendampingan dilakukan secara berkala setiap minggu hingga lele bisa dipanen. Evaluasi dilakukan di akhir kegiatan untuk mengetahui efektivitas dan potensi teknologi untuk dikomersilkan. Hasil dari program ini menunjukkan bahwa budidaya lele dan kangkung menggunakan pot dapat digunakan untuk memenuhi kebutuhan pangan keluarga dengan harga yang murah. Desain teknologi kolam portabel dari pot ini juga berpotensi untuk dikembangkan sebagai industri rumah tangga. Pakan lele dari limbah rumah tangga mampu menekan biaya pakan dalam budidaya lele hingga 20% dengan nilai rasio konversi pakan (FCR) sebesar 1.11 atau efisiens pakan hingga  90%. Pakan lele ini membantu pengurangan sampah sisa makanan dan sampah dapur hingga 100%.
Co-Authors Abdul Rahman Siregar, Abdul Rahman Anjar Tri Wibowo BUDI SETIADI DARYONO Cahyo Wulandari Himawan, Tyas Ikhsan Huda, Muhammad Syafi’atol Kusnanda, Prima Sekti Noviyani, Pipit Nugrahapraja, Husna Priyono, Dwi Sendi Putri, Wahyu Aristyaning Rachman, Mifta Pratiwi Sebastian, Alfino Sofyana, Neng Tanty Sukirno Sukirno Windianingsih, Diah Fitri Yekti Asih Purwestri