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Early Detection and Serotyping of Dengue Viruses Clinical Isolates Using Reverse Transcription Polymerase Chain Reaction (RT-PCR) 2 Primers Siregar, Abdul Rahman; Wibawa, Tri; Wijayanti, Nastiti
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.448 KB)

Abstract

Recently several methods for confirming Dengue Virus have been developed involve virus isolation, detection of virus antigen, and nucleic acid using PCR. It has been reported that rapid detection method for confirming DHF by Multiplex RT-PCR had been successfully developed. It was more effective than the other methods with a high sensitivity and specivicity were 100% at the early phase (day 1-3). This study was designed to develop rapid detection and serotyping methods for Dengue Virus using RT-PCR 2 primers (Dcon and preM) with annealing temperature was 57oC. The whole blood samples were collected from suspected dengue fever patients that had been confirmed with NS1 kit from R.S. Persahabatan DKI Jakarta and R.S. Prof. Dr. Sardjito DI Yogyakarta during Februari-August 2009. The PCR products showed that in 12 samples, 100 % were postitive with different pattern among the serotypes especially for DEN1 and DEN2, but not for DEN3 and Den4.  This method was also able to confirm the double infection DEN2-DEN3, but not for the other ones because of the unspecific pattern. From the results, it indicated that the 2 primers can be a promising early detection and serotyping method of Dengue Virus which infected the DHF patients. Key words: Dengue Virus, DHF, early detection, serotyping, RT-PCR 2 primers.
Green Conservatism dalam Pemikiran Roger Vernon Scruton (1944-2020) Siregar, Abdul Rahman; Junaidi, Junaidi; Siregar, Husna Sari
Hijaz: Jurnal Ilmu-Ilmu Keislaman Vol. 4 No. 1 (2024)
Publisher : Medan Resource Center

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Abstract

This writing aims to address several issues: First, the synthesis of stimuli leading to the birth of Roger Scruton's concept of green conservatism; Second, a critical evaluation of the meaning and principles of Roger Scruton's green conservatism. The findings of this research are as follows: first, the intellectual stimuli that gave rise to Scruton's concept of green conservatism began with Scruton's first formative experience, namely his difficult and contentious relationship with his father. This led young Scruton to tend to appreciate objects that his father disliked. The second stimulus occurred when Scruton was in Paris during the student riots of 1968. Scruton viewed the destructive behavior of the rioting students as highly mischievous, describing the riots as a case of young people indulging themselves by plundering the heritage that made their lives privileged. The final stimulus came in 1995 when he turned away from academia to focus on writing and farming after purchasing farmland in Wiltshire. Second, Scruton has integrated the principles of conservatism with environmentalism, resulting in green conservatism, a philosophical and political stance that combines traditional conservative values with environmental concerns. This concept emphasizes several important principles, including: oikophilia, subsidiarity, localism, and the preservation of culture and history.
Establishing Geographical Indicator of Fermented Cacao Beans Using Microbiome Fingerprinting Nugroho, Imam Bagus; Siregar, Abdul Rahman
Journal of Biotechnology and Natural Science Vol. 4 No. 1 (2024): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i1.10775

Abstract

Geographical indication is an essential label for industrial products. Herein, we aimed to explore a method for establishing geographical indications based on microbial diversity data. We collected two groups of datasets available on the public server of the European Nucleotide Archive. These datasets contain 12 (twelve) NGS-generated reads (amplicon sequencing metagenomes) of fermented cacao beans from Brazil and Mexico. We extracted the microbiome profile using bioinformatic tools in the SHAMAN server. We analyzed further using Principal Component Analysis, Clustering (Ward’s Method of Hierarchical Agglomerative Clustering), and UMAP (Uniform Manifold Approximation and Projection) combined with KNN (K-Nearest Neighbor). We discovered differences in microbial diversity and unique taxa in the fermented cacao beans from Brazil and Mexico. Lactic acid bacteria (LAB), such as Liquorilactobacillus, Tatumella, Leuconostoc, Companilactobacillus, and Limosilactobacillus, are unique genera in samples from Mexico, while Bacillus is a unique genus found in samples from Brazil.  We have demonstrated the separation of the microbiome profiles between fermented cacao beans from Brazil and Mexico using PCA, clustering analysis and UMAP-KNN. We have successfully developed the proof of concept in establishing geographical indicators based on microbial diversity data or microbiome profiles. In the future, we will extend this research to analyze samples from Indonesia and establish a microbial diversity database of Indonesian fermented cacao. This database is essential for the authentication assay of Indonesian fermented cacao and for developing fine cacao and specialty products.
Design of Laboratory Scale Oil Distillation Machine Satria, Dhimas; Rosyadi, Imron; Irman, Ade; Lusiani, Rina; Haryadi, Haryadi; Siregar, Abdul Rahman
World Chemical Engineering Journal VOLUME 1 NO. 3 DECEMBER 2016
Publisher : Chemical Engineering Department, Engineering Faculty, Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/wcej.v1i3.945

Abstract

In Indonesia there are 4,000 old oil wells are not exploited again by Pertamina becauseit is not economical, some old wells exploited and processed in the traditional way.Their traditional oil processing has not significantly give a role to the income of localcommunities, as the mining process that is not managed by modern technologyresulting in a weak bargaining position. This study aims to participate in the welfare ofthe community in areas rich in oil resources and also support the energy security bydeveloping technologies distillationof crude oil into diesel by exploiting oil wellsparents who are not exploited again by Pertamina that will improve the bargainingposition (bargaining power), increase revenue, reduce unemployment and poverty andhas created a system of social safety nets and insurance against society.The methodused is the method of Pahl and Beitz, which consists of the Planning and TaskClarification, Conceptual Design, Embodiment Design, and Detail Design.The results ofthe research are the best variant for the design of machines fractional distillation ofpetroleum laboratory scale variant 1 (Boiler cylinder - 0.5 inch Pipe - Tubes distillationcylinder - gas stove), by reason of the appropriate size pipe diameter and more securethan the variance other variants. While the dimensions obtained from the draft is 1.31m 2 for a total dimension of the tower area (AT) and 408 mm for diameter Total Tower(DT), and 3 mm for the reactor tube wall thickness. In addition, the test results of oilthat has been processed using distillation machine laboratory scale show still makesdiesel specifications in accordance with standards established by the Government,namely the specifications for specific gravity, viscosity, water content, maximumheating and visual appearance.
Global Perspectives on Environmental Microbiome Research: Current Status and Future Directions Putri, Wahyu Aristyaning; Subiastuti, Aprilia Sufi; Wulandari, Cahyo; Rachman, Mifta Pratiwi; Sebastian, Alfino; Siregar, Abdul Rahman; Himawan, Tyas Ikhsan; Priyono, Dwi Sendi; Sofyana, Neng Tanty; Purwestri, Yekti Asih; Nugrahapraja, Husna; Wibowo, Anjar Tri
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.266

Abstract

The environmental microbiome plays an important role in various ecosystems around the world, influencing nutrient cycling, disease dynamics and ecosystem stability. This bibliometric analysis provides a comprehensive overview of the current state and future directions of environmental microbiome research from a global perspective from 2009–2024. Through systematic examination of Scopus publications, 2154 documents were found. The results show a significant increase in the number of publications since 2017 and a peak in 2024. The most cited document was "The hidden world within plants: Ecological and evolutionary considerations for defining functioning of microbial endophytes" with 1887 citations and “Structure and function of the global ocean microbiome” with 1843 citations. The most productive countries in environmental microbiome research are the United States (n = 748), China (n = 533), India (n = 308), Germany (n = 172) and the United Kingdom (n = 157). Microbiome, microbial community, microfolora, microbiota, microbiology, bacteria, and bacterium are the most popular topics that will continue to develop in the future. Although the United States has published the highest total number of papers, more recent studies have predominantly been published from China, indicating shift in the centre of study from the United States to China. Insights gained from this analysis contribute to a deeper understanding of the environmental microbiome research landscape, guiding future research priorities and collaborations in the field.
Antibacterial Activity of Bacterial Pigment Extracts Isolated From Fruit and Vegetable Waste Against Staphylococcus epidermidis Salsabila, Haniya; Maharani, Andrea; Siregar, Abdul Rahman
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.37936

Abstract

Acne affects approximately 9.4% of the global population and become one of the big eight skin diseases due to Staphylococcus epidermidis infection. This infection can be treated using bacterial pigments for their potential activities as antioxidant, anticancer, and antimicrobial with low toxicity and stable productivity. In this study, pigments were harvested and purified from pigment-producing bacteria which were isolated from fruit and vegetable waste, and the antibacterial activity was conducted with disc diffusion method against S. epidermidis. There were three pigment-producing isolates (LBS 6, LBS 12, and LBS 14) that produced green pigments with antibacterial activity against S. epidermidis. Among the pigments produced by the three isolates, pigments from LBS 14 had the widest zone of inhibition and the strongest antibacterial activity followed by LBS 6 and LBS 12 respectively. In addition, through Two-Way ANOVA analysis, it was found that there was a significant effect on the utilization of pigments from different bacterial isolates and variations in concentration on the diameter of the inhibition zone as well as the interaction between them