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UJI KARAKTERISTIK SEDIAAN SALEP EKSTRAK ETANOL BUAH MAKASAR (Brucea javanica [L.] Merr) SEBAGAI KANDIDAT SALEP UNTUK LUKA INCISI DAN LUKA DIABETES Jacob, Jois Moriani; Oematan, Aven B.; Maakh, Yorida Febry
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.6614

Abstract

Makasar fruit (Brucea javanica [L]. Merr) has been widely used as herbal medicine to treat various types of diseases through simple processing. However, the use of Makasar fruit drugs with topical application as an ointment to treat incision wounds or diabetic wounds is extremely rare. The purpose of this research is to produce ointment and to assess the physical quality of the ointment made from the ethanolic extract of the Makassar fruit. The ointment used in this study was made with ethanol extract of Makassar fruit, alpha tocopherol, propyl-paraben, and Vaseline album. The data were observed descriptively and using a qualitative method. This study used two ointment concentrations, 15% and 20%, which were tested three times for each physical evaluation (organoleptic test, homogeneity test, dispersibility test, and pH test). The results showed that the ethanol extract of Makassar fruit could be formulated as an ointment, and the physical evaluation of the ointment discovered that the ointment met the organoleptic standards and the homogeneity standards. Furthermore, the pH test revealed that the ointment was safe to use; however, only 1 sample (repeat 1 concentration of 20%) confirmed that it did not meet the pH requirements because it was lower than the standard (acid pH). Furthermore, the dispersion tests indicated that the ointment did not meet the ointments dispersion standard. Conclusion: because the physical examination showed that the ointment met the standard values, the ointment can be made from the ethanol extract of Makassar fruit. However, because the ointment's spreadability is below the standard value, it must be taken into account.
Analisis Metabolit Sekunder Daun Brucea javanica [L] Merr dari Dua Tempat Berbeda di Pulau Timor Yasa, Julita Dewitri Mertha; Jacob, Jois Moriani
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.15558

Abstract

The leaves of Brucea javanica [L] Merr have long been utilized as an alternative medicine for a variety of illness. Although a number of investigations have been conducted to ascertain the advantages and composition of secondary metabolites in this plant's leaves, nothing has ever been published on the particular study conducted on the island of Timor. The purpose of this study was to ascertain which secondary metabolites were present in the leaves of this plant that was grown in two distinct locations on Timor Island. The findings of this study will be used as an empirical basis for future investigations into the use of herbal remedies. The study's findings demonstrated that while flavonoids and alkaloids weren't present from the plant's ethanol extract, it did contain highly concentrated terpenoids and tannins at both locations. In contrast, saponin content was only discovered in leaves collected from the neighbourhood of Fatumnutu. The spectrophotometer test revealed that the ethanol extract of Bruce planta javanica [L] Merr from Noelbaki Village had a greater secondary metabolite absorption value compared to plant extract from Fatumnutu Village. The absorption values for tannin and alkaloid showed the largest overall variation in absorption levels, varying by around 0.513% and 0.68% for each constituent. In the meantime, there is a 0.513% variation in tannin compounds between the two sites. In order to ascertain the bioactivity of this plant's leaf extract for the creation of herbal medicines in Indonesia, more investigation is required into the variations in saponin levels and absorbency levels of secondary metabolite compounds from the two locations.
Ethanol Extract of Makassar Leaves (Brucea javanica [L.] Merr.) as a Potential Biopesticide against Rhipicephalus sanguineus in Dogs Jacob, Jois Moriani; Oematan, Aven B.
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20086

Abstract

Rhipicephalus sanguineus adalah ektoparasit yang sering menyerang anjing, menyebabkan masalah kesehatan seperti anemia, iritasi kulit, dan penurunan berat badan. Penggunaan obat sintetis seperti pyrethroid efektif tetapi memiliki efek samping berbahaya bagi hewan, manusia, dan lingkungan. Oleh karena itu, penelitian ini bertujuan mengevaluasi ekstrak etanol daun makassar (Brucea javanica [L.] Merr.) sebagai biopestisida alami untuk mengendalikan R. sanguineus, mengingat kandungan tanin dan terpenoidnya yang berpotensi sebagai antiparasit. Penelitian ini dilakukan di Laboratorium Kesehatan Hewan Politeknik Pertanian Negeri Kupang dari bulan Mei sampai November 2022. Daun Brucea javanica diekstraksi menggunakan etanol 96% dan diuapkan hingga terbentuk ekstrak semipadat, kemudian diencerkan menjadi konsentrasi 10%, 20%, dan 30%, dengan kontrol positif Cypermethrin 100 g/L dan kontrol negatif aquades. Sebanyak 120 kutu dibagi ke dalam kelompok perlakuan dan diamati mortalitasnya setiap jam selama 8 jam. Data dianalisis menggunakan ANOVA dan uji lanjut Duncan. Hasil menunjukkan bahwa semua konsentrasi ekstrak (10%, 20%, dan 30%) menyebabkan mortalitas kutu yang signifikan (P<0,05) dibandingkan kontrol negatif, meskipun tidak berbeda signifikan dengan kontrol positif. Waktu penyemprotan optimal adalah 120 menit, dengan mortalitas tertinggi (1,80±2,111). Efektivitas ekstrak diduga berasal dari kandungan terpenoid yang mengganggu sistem saraf kutu dan tanin yang menghambat metabolisme. Namun, peningkatan konsentrasi tidak secara signifikan meningkatkan mortalitas, menunjukkan bahwa efek ekstrak mungkin telah mencapai titik optimal pada konsentrasi rendah. Ekstrak etanol daun Brucea javanica terbukti efektif sebagai biopestisida terhadap R. sanguineus, terutama pada waktu penyemprotan 120 menit. Namun, diperlukan penelitian lebih lanjut untuk mengisolasi senyawa aktif dan menguji efikasi pada dosis yang lebih tinggi serta metode aplikasi yang lebih baik. Temuan ini menawarkan alternatif alami yang potensial untuk mengurangi ketergantungan pada pestisida sintetis.
Analysis of SGPT and SGOT Levels in Free-Range Chickens Given Starfruit Leaf Extract Moenek, Devi Y. J. A.; Jacob, Jois Moriani; Toelle, Novianti N.; Yasa, Julita D. Mertha; Manoh, Olivia
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20718

Abstract

Tujuan dari penelitian ini adalah untuk menentukan kadar enzim Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Serum Glutamic Pyruvate Transaminase (SGPT) pada ayam kampung yang diberi ekstrak daun belimbing wuluh (Averrhoa bilimbi L.). Ekstrak daun belimbing wuluh mengandung metabolit sekunder seperti tanin, saponin, dan flavonoid, yang semuanya memiliki potensi untuk berfungsi sebagai antibakteri alami dan imunostimulan pada unggas. Studi ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima kelompok perlakuan: tiga kelompok ayam yang diberikan ekstrak daun belimbing wuluh dengan dosis 0,25 mL, 0,30 mL, dan 0,35 mL per ekor per hari, dan dua kelompok kontrol (positif untuk antibiotik oksitetrasiklin dan negatif untuk air). Sampel darah diambil setelah periode perlakuan, dan kadar SGOT serta SGPT diukur di laboratorium. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun belimbing tidak memiliki efek signifikan terhadap kadar SGPT ayam kampung (P>0,05). Namun, kadar SGOT bervariasi antar kelompok, dengan nilai tertinggi pada kontrol negatif. Ini menunjukkan bahwa ekstrak daun belimbing tidak bersifat hepatotoksik dan dapat digunakan sebagai alternatif antibiotik alami. Temuan ini mendukung penggunaan ekstrak daun belimbing wuluh dalam pakan ayam kampung sebagai cara untuk mengurangi ketergantungan pada antibiotik sintetis tanpa berdampak negatif pada fungsi hati.
Anatomical and Histological Features of the Tongue Wild Boar (Sus scrofa) from Timor Island Harum, Aurelia Bebonita; Inggrid Trinidad Maha; Nitbani, Heny; Filphin Adolfin Amalo; Selan, Yulfia Nellymalik; Jacob, Jois Moriani
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p875-888

Abstract

The tongue is a complementary organ owned by living things that easily moves and fills the oral cavity when the upper and lower jaw teeth meet. This study aims to determine the anatomical structure and histology of the tongue in wild boar (Sus scrofa) from the Island of Timor. Tongue organs were collected from three healthy wild boars. Animals were slaughtered and necropsied. Macroscopic observations were made; length, width, and thickness were measured, and the tongue tissue was cut into several parts, namely the apex lingua, corpus lingua, and radix lingua, to be fixed in 10% formalin. Furthermore, Hematoxylin-Eosin (HE) staining was performed. The results showed that the dorsal surface has four types of papillae, namely circumvallate papillae, conical papillae, foliate papillae, fungiform papillae, and filiform papillae. The tongue muscles of wild boar were found to be m. longitudinalis, m. transversus, and m. verticalis. Conical papillae are conical with a blunt tip. Foliate papillae are located on the lateral-radix of the tongue. Fungiform papillae have a dome-like oval shape. Filiform papillae have an elongated cylindrical shape with a blunt tip. The size of the filiform papillae at the corpus of the tongue is larger than at the apex of the tongue.