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All Journal Jurnal Farmasi Sains dan Komunitas Jurnal Pengabdian Masyarakat untuk Negeri (UN-PENMAS) Jurnal Farmasi Indonesia

Yunita, Oeke

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Author-ID : 1295842

Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Cytotoxicity Assay of Dètente Instantanée Côntrolée Pre-Dried Pandanus conoideus Lam. Extracts Yunita, Oeke; Kohar, Indrajati; Allaf, Karim; Anggara, Aris Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 13 No. 1 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Red fruit (Pandanus conoideus Lam.) contains antioxidant compounds in high levels, including beta-carotene and tocopherol, which neutralize free radical compounds which can cause cancer. Red fruit contains 46% water so red fruit can only last 5 days in this condition. To maintain its stability, it is necessary to dry it with various methods, for example détente instantanée contrôlée (DIC) is a revolutionary pressure drop drying procedure. Every procedure for drying raw material can affect its quality and activity, for example the anticancer activity of red fruit. In-vitro cytotoxicity of DIC pre-dried red fruit extracts on HeLa cells has never been tested, so it is important to study. Red fruit which was obtained from Klamono, Sorong, a highland in Papua was extracted with ethanol or hexane which previously dried by DIC pre-drying or conventional drying methods were weighed then dissolved in DMSO. Cytotoxicity assays was conducted using method the MTT with five levels of extract concentration. Result of this research showed that cytotoxicity assay of red fruit extracts on HeLa cells showed that red fruit extract can inhibit cell viability. The conventionally dried red fruit extract shows stronger cytotoxicity against HeLa cells than the DIC pre-dried extract. Red fruit extract cytotoxicity against HeLa cells: ethanolic extract of red fruit previously dried by conventional drying technique > ethanolic extract of red fruit previously dried by DIC pre-drying process > hexane extract of red fruit previously dried by conventional drying method > hexane extract of red fruit previously dried by DIC pre-drying process.

Karakterisasi Morfologis dan Molekuler Varietas Kedelai (Glycine max) untuk Identifikasi Bahan Baku Obat Tradisional Indonesia Wiradjaja, Fuad Soegibudiono; Yunita, Oeke
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 13 No. 2 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Many nutrients and active medicinal compounds in soybean (Glycine max) reduce the risk of cancer, cardiovascular disease, postmenopausal issues, diabetes, and some neurodegenerative disorders. Soybean protein causes several allergic reactions, which necessitates quality control for traditional medicines and herbal products. Morphological and molecular characterization using Random Amplified Polymorphic DNA (RAPD) method were used to control soybean quality. Morphological characterization of 35 soybean varieties from the Research Institute for Various Beans and Tubers (BALITKABI), Malang, East Java, included photos of each variety and seed size, color, hilum color, weight, and special characteristics. RAPD with OPF-3 and OPF-16 primers characterized 35 soybean seed varieties. This study found that morphological characteristics like seed color, hilum color, seed shape, and seed weight are difficult to use to distinguish between varieties, especially if there are no specific features that predominate in these varieties. However, molecular authentication with RAPD can distinguish 35 soybean varieties by the presence of specific polymorphic bands that can only be found in certain varieties.

Co-Authors Allaf, Karim Anggara, Aris Sri Hendra Dinata Indrajati Kohar Krisnawan, Alfian Hendra Prianka, Dian Savitri, Melania Dwi Sujoko Efferin Wiradjaja, Fuad Soegibudiono

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