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All Journal Medali Jurnal : Media Dental Intelektual Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association Odonto dental journal
Rahman, Friska Ani
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Ethanol extract of soursop leaf inhibits acid production and adhesion of Streptococcus mutans Rahman, Friska Ani; Intan, Rahsunji
Padjadjaran Journal of Dentistry Vol 33, No 3 (2021): November
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no3.29385

Abstract

Introduction: Dental caries and dental plaque are the most popular global oral health problems. The primary step of dental caries is characterized by damage of tooth surfaces  affected by acids which are by-products of sugar metabolism by a cariogenic bacteria. One of the cariogenic bacteria is Streptococcus mutans. Annona muricata is traditionally applied as an herbal remedy for various illnesses and has been recognized in a previous study as an antimicrobial agent. This study was aimed to determine  inhibition of ethanol extract of soursop  on acid production and adhesion of Streptococcus mutans. Methods: Soursop leaf extracted by maceration using 70% ethanol solvent. The extracts obtained were tested at various concentrations. To examine the effect of ethanol extract of Soursop on acid production by S. mutans, the pH of the culture was determined using a pH meter. Inhibition of adhesion of S. mutans to the saliva-coated hydroxyapatite (S-HA) discs was quantified using colony counting on TYS20B agar plates. Results: Ethanol extract of soursop showed  significant inhibition of acid production at the concentrations of 50, 75, 100, 125 and 150 mg/ml compared to the negative control group. The pH of S. mutans cultures in the presence of ethanol extract of Soursop leaf at various concentrations was higher than negative control, but there were no differences in pH value between the various concentrations of ethanol extract of Soursop leaf. The extract clearly inhibited S. mutans adhesion to saliva coated hydroxyapatite beads at the concentration  50, 75, 100, 125 and 150 mg/ml compared to the negative control group. Adhesion decreased with increasing concentrations of ethanol extract of Soursop leaf, but there was not significant difference in colony count between the various concentrations of ethanol extract of Soursop leaf. Conclusions: The ethanol extract of Annona muricata leaf can inhibit acid production and adhesion of S. mutans.
THE EFFECT OF NANOEMULSION MOUTHWASH CONTAINING CITRONELLA OIL (Cymbopogon winterianus) AND LEMON OIL (Citrus limon) ON THE NUMBER OF MACROPHAGES IN PREGNANT RAT MODEL INDUCED BY GINGIVITIS Rahman, Friska Ani; Sunarintyas, Siti; Syaify, Ahmad
Jurnal Medali Vol 7, No 2 (2025): Media Dental Intelektual August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.7.2.162-168

Abstract

Background: Pregnancy gingivitis is known to affect high-risk pregnancies. Therefore, preventive treatments are necessary, one of them being use of  mouthwash. Citronella oil and lemon oil have been proven to have anti-inflammatory properties. The aim of this study was to investigate the effectiveness of a nanoemulsion mouthwash combining citronella oil (Cymbopogon winterianus) and lemon oil (Citrus limon),  on macrophage counts in a pregnant rat model induced with gingivitis. Method: Female Sprague Dawley rats were confirmed to be pregnant, followed by ligation of both lower incisor teeth, and then mouthwash application. The rats were routinely fed a moist diet and drinking water containing 10% sucrose. Mouthwash application was performed twice daily (morning and evening) over 7 day. Histological evaluation was conducted by counting the number of macrophages in hematoxylin-eosin preparations. Result: The results showed a reduction in the number of macrophages with the administration of the nanoemulsion mouthwash combining citronella oil and lemon oil. The Mann -Whitney test revealed a significant difference compared to the control group (p<0.05). Conclusion: The nanoemulsion mouthwash with 1% citronella oil and 2,77% lemon oil may help reduce the number of macrophages in pregnant rats with gingivitis.
The Effect of Porphyromonas gingivalis Lipopolysaccharide-Induced Periodontitis in Rats Fed a High-Cholesterol Diet on Macrophage Number Sari, Finsa Tisna; Tandelilin, Regina Titi Christinawati; Rahman, Friska Ani; Mardhiyah, Iffah; Widita, Elastria
Journal of Indonesian Dental Association Vol 4 No 2 (2021): October
Publisher : Indonesian Dental Association

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Abstract

Introduction: Periodontitis is a chronic inflammatory condition of the tooth-supporting tissue. P. gingivalis, which produces virulence factors, including lipopolysaccharide (LPS), is the main pathogenic driver of periodontitis. However, the interaction between the innate immune system and periodontal pathogens in hyperlipidemia remains unclear. Objective: The aim of this study was to investigate the effect of a high-cholesterol diet (HCD) on macrophage activity in P. gingivalis LPS-induced periodontitis. Methods: Twenty-eight male Sprague Dawley rats were divided into four groups (n=7 rats each group): LPS-HCD, saline-HCD, LPS-basal diet (LPS-BD), and saline-BD. HCD group had been being feeding by high cholesterol diet (1% cholesterol (w/w) and 0.5% cholic acid (w/w)) for 30 days before were injected with 0.2 ml of P. gingivalis ATCC 3277 LPS (LPS-HCD group) and saline (saline-HCD group). The other two groups had been being feeding by normal basal diet for 30 days before were injected with 0.2 ml of P. gingivalis ATCC 3277 LPS (LPS-BD group) and saline (saline-BD group). Rats were sacrificed and lower jaws were harvested and embedded in paraffin. Paraffin section of lower right and left incisor were deparaffinized, rehydrated, and stained with hematoxylin & eosin (H&E). The total number of macrophages was counted using a light microscope at a magnification of 400× from 10 fields of view. Results: The number of macrophages in the LPS-HCD group was the highest compare to LPS- BD, saline-HCD, and saline-BD groups. In addition, LPS-BD group had higher number of macrophage than saline-BD group which had the lowest number of macrophages. Conclusion: HCD and P. gingivalis LPS-induced periodontitis can contribute to increasing of macrophage activity in periodontitis. Thus, HCD itself can enhance the process of inflammation in periodontitis.
THE EFFECT OF SOURSOP (ANNONA MURICATA L.) ESSENTIAL OILS ON VIABILITY CELLS: AN IN-VITRO STUDY Rahman, Friska Ani; Naday, Qotru Al; Utami, Trianna Wahyu
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (735.772 KB) | DOI: 10.30659/odj.8.1.28-33

Abstract

Background: Development of new preventive agents for dental caries is needed. One of the candidates for preventive agents from natural products is Soursop leaf. The present study aimed to determine the effect of Soursop leaf oil on the cultured epithelial and fibroblast cells.Methods: In this experimental study, Soursop leaf essential oils were provided, and their effect was discovered on epithelial and fibroblast cells line using MTT assay. The MTT assay was conducted to measure the activity of enzymes that reduce MTT and switch it to formazan dye creating a purple colour. Using a microplate reader, the optical density was measured at 550 nm and the absorbance value directly represented relative cell numbers.Results: Data compilation and analysis were done using one-way analysis of variance. Soursop leaf essential oils exhibited variable noxious effects on cultured cells. The present study shows that epithelial cell death was less than 30% at the concentration 2.5 µl/ml while the percentage of fibroblast cell death was less than 30% at smaller concentrations of 1.25 µl/ml. Through an increase in the concentration of Soursop leaf essential oils, the toxicity of these materials substantially increased (p<0.05)Conclusion: Soursop leaf essential oils at certain concentrations may cause epithelial and fibroblast cell death.
Co-Authors Ahmad Syaify, Ahmad Elastria Widita Finsa Tisna Sari, Finsa Tisna Iffah Mardhiyah Intan, Rahsunji Naday, Qotru Al Regina Titi Christinawati Tandelilin Siti Sunarintyas Trianna Wahyu Utami