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Pahlevi, Dylan R.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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AMPLIFIKASI GEN COI DARI SAMPEL DARAH ULAR DENGAN MENGGUNAKAN BEBERAPA PASANGAN PRIMER UNIVERSAL Pahlevi, Dylan R.; de Queljoe, Edwin; Kolondam, Beivy J.
ZOOTEC Vol 39, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.387 KB) | DOI: 10.35792/zot.39.2.2019.25413

Abstract

AMPLIFICATION OF COI GENE FROM SNAKE BLOOD SAMPLES USING TWO UNIVERSALPRIMER PAIRS. This study aims to amplify COI (Cytochrome Oxidase Subunit I) gene fragments from snake blood. Four samples were obtained from four different snake individuals that were captured in Tapahan Telu Waterfall, Kali Village, Minahasa Regency. Total DNA from the sample was isolated and then the COI gene was amplified through the PCR (Polymerase Chain Reaction) reaction using two primer pairs, LCO1490-HCO2198 and FF2d-FR1d. These four samples were successfully amplified using different primers, i.e. DRP1 and DRP3 by FF2d-FR1d and DRP2 and DRP4 by LCO1490-HCO2198 primers. The success of amplification marked by the presence of 710 bp (LCO1490-HCO2198) and 707 bp (FF2d-FR1d) bands, which were indicated by those bands were located close to the standard 750 bp DNA ladder. Key words: Snake, Amplification, COI gene, Primers, PCR
Identifikasi Trichoderma sp. dari Rizosfer Tanaman Kanonang (Chordia dichotoma. G. Forst) dan Karakteristik Pemacu Pertumbuhan serta Daya Antagonismenya Terhadap Fusarium oxysporum Rawis, Billy David; Pahlevi, Dylan R.; Siahaan, Parluhutan; Singkoh, Marina; Tangapo, Agustina
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.60630

Abstract

The use of biological control agents to inhibit the growth of pathogens in this case Fusarium in agricultural plants is very important in order to reduce the impact of environmental damage. Trichoderma sp. is a fungus that can be obtained from the rhizosphere of plants that can increase soil fertility and inhibit pathogenic fungi that cause damage to agricultural plants. The use of local isolates of antagonistic fungi in this case from the rhizosphere of the Kanonang plant gave results where the discovery of Trichoderma species that are able to control plant pathogens in this case Fusarium oxysporum through antagonism mechanisms such as inhibition, antibiosis and microparasitism. To find out the species of Trichoderma sp, it was continued with a molecular test using the ITS marker gene and the results of the test confirmed that the Trichoderma species in the rhizosphere of the Kanonang plant was the Trichoderma asperellum type. This type of Trichoderma also after being tested for its characteristics in stimulating growth gave positive results for both phosphate solvent and auxin producer (IAA) tests
Co-Authors Agustina Tangapo BEIVY JONATHAN KOLONDAM Edwin de Queljoe Marina F. O. Singkoh Parluhutan Siahaan Rawis, Billy David