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All Journal Jurnal MIPA Jurnal Penelitian Pendidikan IPA (JPPIPA)
Agustina Tangapo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Mathematics Physics

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Potensi Metabolik Komunitas Bakteri Endofit dan Rhizosfer Ubi Jalar (Ipomoea batatas L.) Berdasarkan Analisis Community-Level Physiological Profiles (CLPP) Tangapo, Agustina
Jurnal MIPA Vol 9, No 1 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.9.1.2020.27159

Abstract

Aktivitas metabolik dari komunitas bakteri endofit dan rizosfir diukur untuk membandingkan tingkat community-level physiological profiling (CLPP) menggunakan Biolog Ecoplates. Hasil penelitian kami menunjukkan kepadatan bakteri meningkat dengan urutan: endofit<rhizosfer. Keragaman mikroba dinilai oleh kekayaan spesies, jumlah spesies dan indeks kesamaan. Aktivitas total mikroba tertinggi diamati pada mikroba rhizosfer. CLPP menunjukkan bahwa bakteri bisa memanfaatkan semua kelompok sumber karbon sebagai berikut karbohidrat, asam amino, asam karboksilat, polimer, amina/amida, dan senyawa fenolik. Karbohidrat yang paling dimanfaatkan.  Total metabolic activity of endophytic and rhizosphere of bacterial community of sweet potato was measured to compare the community-level physiological profiles (CLPP) using by Biolog Ecoplates. Result of our study revealed that bacterial density increased in the following order: endophytes < rhizosphere. The microbial diversity assessed by species richness, the total number of species present and species evenness. The highest total microbial activity was observed for the rhizosphere. The CLPP revealed that the bacteria could differentially utilize all the groups of carbon sources as follows carbohydrate, amino acid, carboxilyc acid, polymer, amine/amide, and phenolic compound. Carbohydrate was most utilized.
Molecular Identification of Symbiont Bacteria on Brown Alga Padina australis Hauck Singkoh, Marina Flora Oktavine; Kuron, Sebrina; Umboh, Stella Deiby; Katili, Deidy Yulius; Kolondam, Beivy; Tangapo, Agustina
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.11978

Abstract

The research aimed to identify the symbiont bacteria in brown alga Padina australis from the coastal waters of Molas, Manado city, Indonesia. Fresh samples were crushed with a mortar and pestle until smooth. A total of 1 g of the smooth alga was added into a test tube containing 9 mL of distilled water to obtain a 10-1 dilution. Next, 1 mL of a 10-1 solution was put into another test tube containing 9 mL of distilled water and vortexed for 15 minutes to obtain 10-2 dilution and so on until a 10-8 solution was obtained. 100 μL of solution from each series 10-4 – 10-8 was spread on NA media and incubated at a temperature of 27–29°C for 24 hours. DNA extraction was carried out following the modified Genomic DNA Mini Kit (Geneaid) protocol. DNA amplification was carried out using the 16S rRNA gene. Amplification using primers for the 16S rRNA gene BKXF (forward) and BKXR (reverse) resulted in fragments 1152 base pairs in length. The results of BLAST analysis of SK1 symbiont bacterial isolates found a Max score and total score of 1369, a query coverage of 97%, and a Percent Ident of 97.73%, which showed similarity to bacterial nucleotides of Bacillus cereus.
Co-Authors BEIVY JONATHAN KOLONDAM Deidy Yulius Katili Kuron, Sebrina Singkoh, Marina Flora Oktavine Umboh, Stella D.