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Ginting, Elvi L.
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Environmental Science

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Penambahan madu dalam pengenceran sperma terhadap motilitas spermatozoa, fertilisasi dan daya tetas telur ikan Patin Siam, Pangasius hipophthalmus Marthin, Lutfi; Watung, Juliaan Ch.; Kalesaran, Ockstan J.; Ginting, Elvi L.; Sinjal, Hengky J.; Salindeho, Indra R.N.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20625

Abstract

This research aimed to determine the optimal doses of honey addition on the sperm dilution to optimize spermatozoa motility, fertilization and hatchability of Patin Siam  eggs (Pangasius hypophthalmus).  The experimental design used was Complete Random Design (RAL).  Diluent solution consisted of a physiological NaCl 0.9% and honey.  Treatment doses of honey in aqueous diluent sperm tested as treatments were A (0 mL), B (0.2 mL), C (0.4 mL) and D (0.6 mL), each with three replications.  Observations made included spermatozoa motility, fertilization and eggs hatchability.  The research results showed that the addition of honey had a significant influence on motility,  fertilization, and eggs hatchability. This study concluded that the addition of honey in sperm diluent solution with a dose of 0.6 mL of honey in 99.4 mL of NaCl could increase the spermatozoa motility and  fertilization of Patin siam. Keywords:  honey, motility, fertilization, hatchability,  Pangasius hipophthalmus
Penggunaan bakteri probiotik untuk pencegahan infeksi bakteri Streptococcus agalactiae pada ikan Nila, Oreochromis niloticus Umasugi, Aisin; Tumbol, Reiny A.; Kreckhoff, Reni L.; Manoppo, Henky; Pangemanan, Novie P.L.; Ginting, Elvi L.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20556

Abstract

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed.  Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus  agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of  survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria,  nile tilapia,  Streptococcus  agalactiae, growth,  infection
Co-Authors Hengky J. Sinjal, Hengky J. Henky Manoppo Indra R.N. Salindeho Juliaan Ch. Watung Kreckhoff, Reni L. Marthin, Lutfi Ockstan J. Kalesaran Pangemanan, Novie P.L. Reiny A. Tumbol Umasugi, Aisin