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All Journal e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX
Indra R.N. Salindeho
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Environmental Science

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Penambahan madu dalam pengenceran sperma terhadap motilitas spermatozoa, fertilisasi dan daya tetas telur ikan Patin Siam, Pangasius hipophthalmus Marthin, Lutfi; Watung, Juliaan Ch.; Kalesaran, Ockstan J.; Ginting, Elvi L.; Sinjal, Hengky J.; Salindeho, Indra R.N.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20625

Abstract

This research aimed to determine the optimal doses of honey addition on the sperm dilution to optimize spermatozoa motility, fertilization and hatchability of Patin Siam  eggs (Pangasius hypophthalmus).  The experimental design used was Complete Random Design (RAL).  Diluent solution consisted of a physiological NaCl 0.9% and honey.  Treatment doses of honey in aqueous diluent sperm tested as treatments were A (0 mL), B (0.2 mL), C (0.4 mL) and D (0.6 mL), each with three replications.  Observations made included spermatozoa motility, fertilization and eggs hatchability.  The research results showed that the addition of honey had a significant influence on motility,  fertilization, and eggs hatchability. This study concluded that the addition of honey in sperm diluent solution with a dose of 0.6 mL of honey in 99.4 mL of NaCl could increase the spermatozoa motility and  fertilization of Patin siam. Keywords:  honey, motility, fertilization, hatchability,  Pangasius hipophthalmus
Tingkat keberhasilan hidup teripang gamat lumpur, Stichopus hermanni, hasil dari transverse fission Tatalede, Putri A.; Salindeho, Indra R.N.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20491

Abstract

The aim of the experiment was to find out the survival rate of the sea cucumber (Stichopus hermanni) seeds generated from transverse-fission method, and cultured in the waters of Talengen gulf, Sangihe Islands Region.  Twenty five specimens of sea cucumber were collected around the waters of Talengen gulf and Pananuareng bay, the Region of Sangihe Islands.  The specimens were transported by boat to the field-research area of Nusa Utara Polytechnic, as the site of the experiment.  The specimens were kept individually in 25 plastic mesh cages, where each cage measured 40cmx40cmx40cm.    The individual body length of the specimens was measured  and then  was transversely  cut at the middle of the body antero-posteriorly.  The procedure was conducted underwater to prevent  expelling of the visceral organs.  25 individuals were applied transverse-fission,  hence 50 new individuals were generated, which were composed of 25 Anterior individuals (A) and 25 Posterior-individuals (P).   After transverse fission,  the Anterior- individuals (A) and Posterior-individuals (P), generated from the same individual were put back to their previous cage.  The new individuals were cautiously controlled, and at the interval of 6 hours observation on the sealing process of the open wound and the regenerating process were carried out. The result showed that the survival rate of the sea cucumbers generated from transverse-fission method, was as high as 70.83%.  The Posterior-individuals (P) had higher survival rate (75.66%) than Anterior-individuals (66%).   The result also showed that A and P individuals generated from larger individuals had lower survival rate than those from smaller individuals.   Small individuals sizing 6-14cm had highest survival rate, which was 100%, while larger individuals sizing 14-22cm and 22-30cm had lower survival rate which were 85.5%  and  27% respectively.     The sealing of the open wounded took averagely 4 days, while the process of the regeneration took around 4 weeks. Keywords: sea-cucumber, fission, survival-rate
Pengaruh perbedaan jenis pakan terhadap pertumbuhan lobster laut, Panulirus versicolor Makasangkil, Lestari; Salindeho, Indra R.N.; Lumenta, Cyska
e-Journal BUDIDAYA PERAIRAN Vol 5, No 3 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.3.2017.17608

Abstract

The aim of the research was to find out the growth rate of the marine lobster, Panulirus versicolor, fed different types of diets.   The experiment was conducted at the Laboratory of Aquaculture Technology, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University.  The experiment was set up  in 3 treatments, which were three different types of diet: treatment-A: papaya leaves, treatment-B: coconut flesh, treatment-C: skipjack-fish flesh.  Each treatment was triplicated.  The tested lobsters were collected from the marine waters of the Likupang village, North Minahasa Regency.  The tested lobsters were weighed at the beginning,  at the end of the experiment, and at the interval of 14 days during the experiment period. The data of the body weight change were statistically analysed using the analysis of the homogeneity of the 3 different linear regression coefficients, between time (X) and the body weight of the lobsters (Y) fed different types of feed, at the confidence test level a = 5% and 1%.  The results show that the body weight of the tested lobsters fed skipjack-fish flesh increased 4 grams after 30 days of the rearing period.  The body weight of the tested lobsters fed papaya leaves decreased 11 g, and the body weight of those fed coconut flesh decreased 3 g after 30 days of the rearing period.  The result of the regression analysis shows, the assumption that the 3 regression coefficients are homogenous is rejected.  In other words, there is a significant difference among the growth rates of the tested lobsters fed different type of diets.  Compared to skipjack-fish flesh, the papaya leaves and coconut flesh significantly slow down the level of the water quality degradation during experiment period.Keywords:  diets, growth, marine-lobster
Maskulinisasi ikan nila (Oreochromis niloticus) melalui pemberian propolis yang dicampur dalam pakan buatan Tatalede, Putri A.; Sinjal, Hengky J.; Watung, Juliaan Ch.; Salindeho, Indra R.N.; Kalesaran, Ockstan J.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.2.2019.23922

Abstract

This study was conducted to determine the optimal dose of propolis for male sex reversal in tilapia larvae through mixing in artificial feed. This research was carried out at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University. The experimental fish was taken from Board of Freshwater Fisheries Aquaculture at Tatelu. As many as 4,000 individuals fish aging 5 days were acclimatized for 2 days in an aquarium measuring 60 x 40 x 40 cm. After the acclimatization process was terminated, the larvae density was set to 100 per aquarium. Furthermore, the fish were fed with treatment feed previously added with propolis with a dose of A (0 mL/kg of feed) as a control, B (0.9 mL/kg of feed), C (1.8 mL/kg of feed), D (2.7 mL/kg of feed) and t E (3.6 mL/kg of feed). Treatment feed was given to larvae twice a day for 28 days. The results showed that masculinization of tilapia using propolis mixed into artificial feed had a very significant effect on the percentage of male tilapia and survival. In this study treatment C (1.8 mL/kg of feed) and treatment D (2.7 mL/kg of feed) resulted in the highest percentage of male tilapia which was 81.33% and 88.00% respectively. This shows that the administration at 1.8 mL/kg in artificial feed has a very real and effective effect on masculinization.Keywords: Oreochromis niloticus, masculinization, sex reversal, propolis
Tingkat kelulusan hidup dan regenerasi teripang gamat lumpur, Stichopus hermanii, hasil metode transverse fission Budikase, Arter; Salindeho, Indra R.N.; Mudeng, Joppy D.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.2.2019.23923

Abstract

The research was aimed to figure out the survival rate and the regeneration rate of  the sea cucumber, Stichopus hermanii, seeds generated from the  transverse fission method.  The research was conducted in the Talengen Gulf, the District of Sangihe Islands, at the field research station of the Nusa Utara State Polytechnic.  The tested animals, Stichopus hermanii, were collected from the waters around the Talengen Gulf.  Specimens of the sea cucumber, lengthened 9 to 15cm, were transported by boat to the site of the experiment and kept in plastic mesh-cages. Fourteen individuals then were transversely tied in the middle of the body antero-posteriorly to induce fission, hence there were 28 new individuals were generated, which were composed of 14 anterior seeds and 14 posterior seeds.  The seeds then were kept in 6 plastic mesh cages, where the anterior individuals were separated from posterior ones.  The cages were placed at the bottom of the sea waters where the sea cucumber might have access to substrate and sea grasses around the cages.   The time required by the newly fissioned cucumbers to seal off the open wound, the wound recovery time, the survival rate of the seeds and the regeneration  rate of the new integument connective tissue were among the data collected during the experiment.   The regeneration rate of the new integument connective tissue was indicated by development of a protuberance at the median of the healed wound.  The results show that the survival rate of the Stichopus hermanii seeds generated from transverse fission method were 100% for 40 days post-fission.   The average time for the newly cut cucumber to seal off the open wound is 4 minutes.  The average time for the seeds either anterior or posterior individuals, to recover the wound is aroung 25-28 days.  The protruberance of the newly regeneration integument connective tissue is appeared at day 30.  There is no significant difference between anterior and posterior seeds in regenerating the protuberance of the new integument connective tissue, after 40 days post-fission.Keywords:  asexual, reproduction, transverse fission, survival rate, regeneration
Pengaruh perbedaan salinitas terhadap pertumbuhan kepiting bakau, Scylla serrata Sitaba, Rifan D.; Salindeho, Indra R.N.; Kusen, Diane J.
e-Journal BUDIDAYA PERAIRAN Vol 5, No 2 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.2.2017.16575

Abstract

The aims of the research were to find out firstly, the growth rate of the mud crab Scylla Serrata) reared under different salinity levels and secondly, the range of the salinity where the mud crab optimally grows.   The experiment was conducted at the Laboratory of Aquaculture Technology, FPIK, UNSRAT, from April to May 2017.  The experiment was set up according to the Randomized Block Design, with 3 treatments, which were 3 different salinity levels, 10 ppt, 20 ppt and 30 ppt.  The tested level of salinity was determined based on the preliminary experiment.  Each treatment was triplicated, and each replicate represents the group of the initial weight of the tested crab, which was 80-89 grams (group-1), 70-79 grams (group-2), and 60-69 grams (group-3).  The tested mud crabs were collected from the estuarine waters of Inobonto river, which has salinity level of 12 ppt.  The tested crabs were weighed at the beginning and at the end of the experiment, and at the interval of 7 days during the experiment period.  The weight of the tested crabs data were converted into Absolute Growth Rate (AGR) and Daily Growth Rate (DGR), which in turn were statistically analyzed using Anova for Randomized Block Design, and then Least Significant Difference Test.  The results of the Least Significant Difference Test show that the absolute and daily growth rate of the tested crabs in the salinity of 10 ppt are not significantly different with those in the salinity of 20 ppt.  While the lowest absolute and daily growth rate of the tested crabs were in the salinity of 30 ppt and are significantly different with those in the salinity of 10 and 20 ppt.  Hence, in order to get a maximal production, the mud crab, Scylla serrata, should be cultured in the waters with a range of salinity between 10 and 20 ppt.Keywords: Mud crab, salinity, growth
Pertumbuhan dan kelangsungan hidup ikan nila Oreochromis niloticus yang dikultur pada sistem akuaponik dengan kepadatan biofilter kangkung yang berbeda Gerung, Pramulya R.A; Mudeng, Joppy D.; Salindeho, Indra R.N.; Longdong, Sammy N.J.; Pangkey, Henneke; Rumengan, Inneke F.M.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.38562

Abstract

This research was conducted to determine the optimal density of kale plants as biofilter in an aquaponic system that can trigger the highest growth and survival of tilapia. The experiment was designed in a completely randomized design (CRD) with 3 treatments and 3 replications so that there were 9 experimental units. The factor tested was the different density of kale plants as biofilter in the aquaponics system, where treatment A: 6 kale plants/0,24m2, B: 12 kale plants/0,24m2, and  K: without kale plants (as control). This study was conducted for 28 days.  Collected data  included weight, length and number of fish, which were then converted to  absolute growth rate (weight and length) , daily growth rate, survival rate and kale plant growth. Data  were analyzed using ANOVA  and then Least Significance Differences (LSD) for  further analysis.   The results  showed that the different density of  kale plants had no significant effect on absolute growth rate (weight and  length)  and daily growth rate, but had a significant effect on the survival rate of fish. The LSD results  showed that density of 6 kale plants/0,24m2 (A) were not significantly different from that of 12 individuals kale plants/0,24m2 (B), but both treatments were significantly different from the control (K).  The range of ammonia  was between 0.29 – 0.91 mg/l, nitrite 0.55 – 1.06 mg/l, and nitrate 10 - 47 mg/l.
Study Of The Carrying Capacity Of The Tutud Lake, Tombatu, South-East Minahasa, For Aquaculture Production, Using [P] Parameter Firamitha Suban; Indra R.N. Salindeho; Novie P. L. Pangemanan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23726

Abstract

The research is aimed to assess the carrying capacity of the Tutud Lake waters,  to ensure a sustainable aquacutural production.  The carrying capacity of the Tutud lake was assessed using the method developed by Beveridge (2004), where several parameters of water quality, aquacultural production, and the physical condition of the lake physic  such as, the dimensions of the Lake, flushing rate, total phosphate [P], total aquacultural production per year and Food Conversion Ratio (FCR), were required in the assessment. Collected data were, then, analyzed using the procedures of calculation, which were combined with the several assumptions and modeling based on the previous research (Beveridge, 2004).  Water sample  for phosphate [P] analysis were collected four times with an interval of 7 days, and undertaken at four different positions which  representing the overall water condition of the lake.  Ammonia, Nitrite and Nitrate content of the waters were measured twice with an interval of 1 month.  Daily fluctuation measurements were undertaken for DO, pH and temperature.  Aquacultural protocols and production data were collected using questionnaire and by direct observation at the farm. The result shows that the average phosphate [P] content of the waters of Tutud lake is as high as 1,068 mg/L, which surpasses the maximum recommended value for waters used for aquacultural production.  DO is at the range of 2,2 – 5,0 mg/L, while temperature ranges from 25,3oC to 29oC, and  pH value varies between 6,38 and 7,89.  Average Nitrite content, 0,004mg/L, and Nitrate content,  4,51mg/L, of the waters of  Tutud Lake are at the safe level for aquaculture.  On the contrary, Ammonia content, 0,34mg/L, and H2S content, 0,681mg/L, of the Lake are at the insecure level for aquaculture animals.  Total aquacultural production per year in Tutud Lake , around 22,5 tons of fish per year, is also surpasses the carrying capacity of the waters of the Tutud  Lake which is only as high as 21,48tonnes.  Hence, the total production for the coming year must be reduced as many as 1,02 tones.Keywords: carrying capacity, water quality, production, Lake Tutud, TombatuABSTRAKPenelitian ini bertujuan untuk mengetahui kapasitas produksi optimal untuk menunjang aktivitas akuakultur yang berkelanjutan di danau Tutud dengan menggunakan parameter fosfor. Penelitian dilakukan di danau Tutud, Tombatu, Minahasa Tenggara, selama tiga bulan yang dimulai dari bulan September 2018 sampai bulan Januari 2019. Penentuan daya dukung untuk menunjang aktivitas akuakultur di danau Tutud menggunakan metode yang diberikan oleh Beveridge (2004), dimana dalam tahapan-tahapan prosedur penentuan daya dukung diperlukan sejumlah data menyangkut kondisi fisik danau, kandungan fosfor [P] perairan, tingkat pergantian air danau (flushing rate), produksi ikan total per tahun serta data operasional akuakultur seperti jenis pakan, jumlah pakan dan FCR. Data tersebut kemudian dianalisis menggunakan tahapan-tahapan kalkulasi yang dikombinasikan dengan beberapa asumsi hasil pemodelan penelitian sebelumnya (Beveridge, 2004). Pengambilan sampel untuk pengukuran fosfor dilakukan empat kali selama penelitian, pada empat titik yang mewakili keseluruhan danau, yaitu lokasi di dekat inlet (titik I), diluar lokasi KJT (titik II), di lokasi KJT (titik III) dan di dekat outlet (titik IV). Pengukuran fluktuasi harian dilakukan untuk parameter kualitas air DO, pH, suhu, sementara amoniak, nitrit, nitrat dan H 2 S diukur dua kali selama penelitian. Data operasional dan produksi akuakultur dikumpulkan menggunakan kuesioner serta observasi langsung.Hasil penelitian menunjukkan rataan kandungan fosfat parairan danau Tutud adalah 1,068 mg/l, sudah melewati batas maksimum baku mutu kualitas air untuk akuakultur. Hasil pengukuran untuk beberapa parameter kualitas air lainnya: DO berada pada kisaran 2,2 – 5,0 mg/l, suhu berada pada kisaran 25,30 0 C – 29 0 C, pH berada pada kisaran 6,38 – 7,89, rataan kandungan amoniak 0,34 mg/l, nitrit 0,004 mg/l , nitrat 4,51 mg/l, serta H 2 S 0,681 mg/l. Total produksi ikan per tahun di danau Tutud, sebesar 22,5 ton, sudah melebihi daya dukung perairan danau Tutud. Hasil analisis daya dukung perairan menurut Beveridge (2004), produksi akuakultur di danau Tutud harus diturunkan sebesar 1,02 ton untuk produksi tahun ke depan.Kata kunci: daya dukung, kualitas air, Produksi, Danau Tutud, Tombatu
Fluktuasi parameter kualitas air dan perkembangan flok pada budidaya ikan Nila (Oreochromis niloticus) dengan sistem bioflok di BPBAT Talelu Franklin R. Safsafubun; Suzanne L. Undap; Indra R.N. Salindeho; Novie P.L. Pangemanan; Juliaan Ch. Watung; Henneke Pangkey
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

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Abstract

This study aims to determine fluctuations in water quality parameters and flock development in tilapia (Oreochromis niloticus) aquaculture using the biofloc system. The water quality parameters studied were temperature, DO (Dissolved Oxygen), pH, ammonia (NH4), nitrite (NO2), and nitrate (NO3). The research was carried out at the Freshwater Aquaculture Fisheries Center (BPBAT) Tatelu, from May 2023 to June 2023, using the case study method. The average temperature during the study was 27.2oC, DO (5.05 mg/L) and pH (5.40). The average NH4 value was 0.58 mg/L. The results of this study indicated that ammonia began to increase from the first day and reached a peak on day 5 with a value of 0.77 mg/L. The average NO2 value was 1.12 mg/L, The results of this study indicated that the increase in NO2 occurred rapidly from day 5 and reached a peak on day 27 with a value of 1.92 mg/L, then began to decline. The average NO3 value was 25.21 mg/L, the NO3 value increased on the 12th day to a value of 35.1 mg/L, and this value began to stabilize even though there was a little pressure. The highest floc density was on the 34th day with a value of 34 ml/L, the lowest was on the 11th day with a value of 4 ml/L and the average floc density was 23 ml/L. Tilapia culture in the biofloc system had an absolute growth of 57.11 grams, a specific growth ratio of 1.5%, feed conversion (FCR) of 1.2, and survival of 97%. Keywords: Fish cultivation, Oreochromis niloticus, biofloc system
Fluktuasi parameter kualitas air di tambak super intensif udang vaname: Studi kasus pertambakan PT. Pillar Persada Parigi, Desa Bajo, Minahasa Selatan Telaumbanua, Beatus H.; Pangemanan, Novie P.L.; Tumbol, Reiny A.; Undap, Suzanne L.; Tumembouw, Sipriana S.; Salindeho, Indra R.N.
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 2 (2024): Juli - Desember
Publisher : Universitas Sam Ratulangi

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Abstract

The aim of this research was to determine the physical and chemical parameters of water quality in vanname shrimp culture in Pond PT. Pillar Persada Parigi, Bajo Village, South Minahasa. This research uses direct measurement methods in the field (In situ) and sample analysis in the laboratory. Water quality parameters measured in the field included temperature, degree of acidity (pH), salinity, dissolved oxygen, while ammonia, nitrite, nitrate, phosphate was analyzed at the Manado Industrial Research and Standardization Center. Water sampling and in-situ measurements were carried out in 4 ponds selected randomly from the ponds that were temporarily operating at the time of sampling. Based on the results of measurements of several physical and chemical parameters of water quality, both directly in the field (in situ) and the results of laboratory analysis of pond water samples from PT Pillar Persada Parigi, Bajo Village, South Minahasa Regency, a temperature range of 27 - 32˚C, salinity of 36 ppt was obtained. , dissolved oxygen 3.63 - 7.8 ppm, acidity degree (pH) 7.6 - 8.6, ammonia content 1 mg/L, nitrite <0.2757 mg/L, nitrate 5 mg/L, phosphate <0 .0027 – 1 mg/L. All parameters measured were still considered suitable for survival and supporting the growth of the vaname shrimp being cultured Keywords: vaname shrimp, dissolved oxygen, ammonia, nitrate
Co-Authors Ali Djamhuri Budikase, Arter Cyska Lumenta Deiske Adeliene Sumilat, Deiske Adeliene Edwin D Ngangi Firamitha Suban Franklin R. Safsafubun Gerung, Pramulya R.A Ginting, Elvi L. Hengky J. Sinjal, Hengky J. Henneke Pangkey Henneke Pangkey Inneke F. M Rumengan Joppy Mudeng Juliaan Ch. Watung Kreckhoff, Reni L. Kusen, Diane Joula Makasangkil, Lestari Mamesah, Jeivana F.T Marthin, Lutfi Mokolensang, Jeffrie F. Novie P. L. Pangemanan Novie P.L. Pangemanan Ockstan J. Kalesaran Pangemanan, Novie P.L. Reiny A. Tumbol Sambali, Hariyani Sammy N.J. Longdong Saragih, Debby Dyanessa Sipriana S. Tumembouw Sitaba, Rifan D. Suzanne L Undap Suzanne L. Undap Tatalede, Putri A. Telaumbanua, Beatus H. Usoh, Yosua