Mantiri, Rose
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Isolasi pigmen karotenoid pada mikroalga Nannochloropsis sp. dengan menggunakan beda pelarut Bawias, Miranti; Kemer, Kurniati; Mantiri, Desy; Kumampung, Deislie; Paransa, Darus; Mantiri, Rose
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 2 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.6.2.2018.20641

Abstract

Penelitian ini bertujuan untuk mengisolasi jenis-jenis pigmen dari mikroalga Nannochloropsis sp. dan mengetahui komposisi pelarut yang dapat memisahkan pigmen secara maksimal. Metode yang digunakan dimulai dari kultur mikroalga Nannochloropsis sp. sampai tahap eksponensial. Ekstraksi dilakukan dengan penambahan aseton dan petroleum eter, selanjutnya ektrak total yang diperoleh, dipisahkan melalui Kromatografi Lapis Tipis dengan pengembang Petroleum Eter (PE):Aseton (80:20), PE:Methanol (95:5) dan Benzena:DE:Methanol (17:2:1). Hasil pemisahan diserap melalui spektrofotometer UV-VIS. Hasil yang peroleh pada semua proses pemisahan, pigmen β- karoten berwarna kuning selalu muncul dengan Rf 1,00. Pigmen lainnya yang dapat diidentifikasi adalah tipeviolaxantin dan tipe astacen. Pelarut PE:Methanol (95:5) mampu memisahkan ekstrak total pigmen lebih banyak dibandingkan dengan pelarut yang lain.
Identifikasi dan siklus hidup chydoridae (Cladocera) di Perairan Sulawesi Utara Pangkey, Henneke; Monijung, Revol D.; Mantiri, Rose; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 6, No 3 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.3.2018.24435

Abstract

The purpose of this study was to identify chydoridae using conservative way, while the observations of the life cycle was done through a microscope to determine the egg production per parent and length of life. Individual chydoridae acclimatized to reach 10 generations (1 month), and 10 females were isolated for the production of seedlings. Based on literatures review, chydoridae used was derived from subfamily Aloninae (Alona spp.); while studies on the life cycle found: the egg size of chydoridae 150.2 ± 22.4 μm, and neonates have an average size of 291.82 ± 3.06 μm, and reached adult size after 2.3 ± 0.5 days, with an average size of 441.22 ± 10.2 μm. Time of embryonic development occured for 1-2 days. On the entire life cycle, only 2 eggs could be produced per individual. Based on this study, the number of eggs produced by the female during its lifetime ranges between 30-60 eggs. Similarly, the length of life of this organism on average 47.2 ± 11.7 days, with the longest lifespan was achieved 60 days. It was found also the maximum size was 1,037 ± 8.5 mm; while the average size of an organism's body was 800.42 ± 56.3 μm.Keywords: chydoridae, identification, life cycle, Alona spp
Seagrass Community Structure at Ratatotok Beach, Southeast Minahasa Regency Purba, Dhebby; Wagey, Billy; Luasunaung, Alfret; Manembu, Indri; Sumilat, Deiske; Mantiri, Rose
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47824

Abstract

The purpose of this research is to know about the type of seagrass, the community structure of seagrass based on the density of type, the density of the relatively, type of frequency, frequency relatively, type of cover, cover relatively, diversity index, dominance and parameter index in surrounding water environment of seagrass at Ratatotok beach. The result of this research is identifying 4 species, namely; Thalassia hemprichii, Enhalus acoroides, Syringodium isoetifolium dan Halodule pinifolia. The value of density is 39 indv/m2, the value type of frequency is 7.9, the value type of cover is 39.15, importance value index had 159.04%. Thalassia hemprichii, diversity index is “medium” with an average value (H’) of 2.63 and a higher importance index with an average value of C of 0.82. Surrounding waters at Ratototok Beach got a “medium” value of seagrass cover value is 39%. Environmental parameters in surrounding waters at Ratatotok beach had a value within; Temperature 31.95 0C, salinity 29.79o/oo, pH 7.62, DO 5.60 mg/L and the type of substrate found is sandy, sandy mud and coral fragments. Keywords: Seagrass, Community structure, Ratatotok beach. Abstrak Tujuan dilakukan penelitian ini yaitu mengetahui jenis lamun, struktur komuntas lamun berdasarkan kepadatan jenis, kepadatan relatif, frekuensi jenis, frekuensi relatif, penutupan jenis, penutupan relatif, indeks keanekaragaman, indeks dominansi dan parameter lingkungan perairan padang lamun yang berada di sekitar Pantai Ratatotok. Hasil yang di peroleh dari penelitian ini teridentifikasi 4 spesies, yaitu: Thalassia hemprichii, Enhalus acoroides, Syringodium isoetifolium dan Halodule pinifolia. Dengan nilai kepadatan 39 ind/m2, frekuensi jenis 7,9, penutupan jenis 39,15, Indeks nilai penting (INP) diperoleh nilai 159,04 %,  pada Thalassia hemprichii, Indeks keanekaragaman “sedang” dengan nilai rata-rata (H’) 2,63 dan indeks dominansi tinggi dengan nilai rata-rata C 0,82. Berdasarkan kategori penutupan lamun yang terdapat di sekitar Perairan Ratatotok dikategorikan “sedang” dengan nilai penutupan 39%. Parameter lingkungan antara lain: suhu 31,95 0C, salinitas 29,79 o/oo, pH 7,62, DO 5,60 mg/l dan jenis substrat yang di temukan adalah berpasir, pasir berlumpur dan pecahan karang. Kata kunci : Lamun, Struktur Komunitas, Pantai Ratatotok