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All Journal Microbiology Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Kesehatan dan Kedokteran
Angky Budianti
Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Evidence Based Case Report: Penggunaan Metode PCR Pada Sputum Untuk Diagnosis Pulmonary Aspergillosis Augustine Natasha; Ricky Fernando; Tubagus M. Kurniadi; Elyn Dohar Idarin Aritonang; Angky Budianti
Majalah Kedokteran Indonesia Vol 69 No 6 (2019): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.69.6-2019-198

Abstract

Background: Pulmonary Aspergillosis is an opportunistic infection in the immunocompromised population. The polymerase chain reaction (PCR) method is expected to be a substitute for sputum mushroom culture with faster and more accurate results. Method: Searched in PubMed, CENTRAL, EbscoHost, and ProQuest from October 1-13 using the keywords “Aspergillosis”, “Pulmonary Aspergillosis”, “sputum”, “PCR”, “sputum fungal culture”. The search results are evaluated using inclusion and exclusion criteria. The selected study is then obtained by the full text and re-evaluated. The final results of the selection were then critically reviewed by validity, importance, and applicability by the three authors. Results: Four studies were obtained, with level of evidence 4, which were then critically reviewed. Three studies used PCR in sputum samples as index tests, while one study used it as a reference test. Output from three studies reported sensitivity and specificity of PCR with sputum samples, while one study reported a proportion of PCR results. Two studies reported PCR sensitivity in sputum samples of 100%, while one study reported a sensitivity of 38%. Two studies also reported specificities above 70%, while one study reported a specificity of 38.5%. Conclusion: Studies show promising values of sensitivity and specificity, especially with results obtained quickly. However, the low level of evidence and its costs are quite expensive, causing detection by the PCR method that cannot be used as a routine diagnostic modality.
Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line FITRIYAH SJATHA; OCTAVIA CHANDRA MUSTIKA; ANGKY BUDIANTI; TJAHJANI MIRAWATI SUDIRO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1505.897 KB) | DOI: 10.5454/mi.13.1.5

Abstract

Dengue infection is a global infectious disease with almost 100 million cases occur annually in over more than 100 endemic countries. Dengue virus (DENV), the causative agent of dengue infection, is an 11 kbp RNApositive strand virus which encode 3 structural and 7 non-structural protein within its genome. Non-structural 1 (NS1) protein of DENV is expressed in the earlier stage of infection and having pathogenic role in disease severity. NS1 gene of DENV serotype-2 Indonesian strain was amplified through PCR method using specific designated primers. NS1 amplicon were then cloned into pUMVC4.a and pcDNA3.1 mammalian expression vector which confirmed through colony PCR and sequencing method. Recombinant pUNS1 and pcNS1 plasmids were transfected into CHO-K1 mammalian cell line with lipid based method. Recombinant NS1 protein expression were analyzed through immunostaining using dengue patient sera and rapid NS1 detection kit. Recombinant pUNS1 and pcNS1 plasmids were successfully constructed and recombinant NS1 protein was expressed in CHOK1 mammalian cell line and shown to be reactive against dengue patient sera. Our recombinant NS1 protein also tend to be released outside the transfected CHO-K1 cells as detected in rapid NS1 detection kit. Recombinant dengue NS1 protein was expressed in mammalian cell line in both intra and extracellularly and shown to be immunogenic.
Demographic, Clinical, and Antibiotic Exposure Factors Associated with Carbapenem-Resistant Acinetobacter baumannii: An Eight-Year Retrospective Study Angky Budianti; Dzaki Fadlurrahman Ayyasy; Mubarokah Mubarokah; Victor Sitorus; Yulia Rosa Saharman; Budiman Bela; Anis Karuniawati
Jurnal Kesehatan dan Kedokteran Vol. 5 No. 3 (2026): Oktober: Jurnal Kesehatan dan Kedokteran
Publisher : Asosiasi Dosen Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56127/jukeke.v5i3.3072

Abstract

Antimicrobial resistance is a major global health challenge, and carbapenem-resistant Acinetobacter baumannii (CRAB) is of particular concern because of its high level of resistance, limited therapeutic options, and substantial burden in healthcare-associated infections. Identifying patient characteristics and antibiotic exposure associated with CRAB is essential to support effective antimicrobial stewardship and infection prevention strategies. Objective: This study aimed to analyze the association of demographic factors, including age and comorbidities, clinical diagnosis or manifestations, and a history of carbapenem use within the previous month with CRAB among clinical isolates examined at the Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia, during 2017–2024. Methodology: This quantitative study employed a retrospective cross-sectional design using secondary laboratory and clinical data. Samples were selected through total sampling from patients with clinical specimens yielding A. baumannii. Descriptive analysis was used to characterize the study population, while Chi-square tests were applied to assess associations between the investigated factors and carbapenem resistance. Findings: A total of 172 A. baumannii clinical cultures were identified, of which 118 (68.6%) were CRAB. Age was significantly associated with CRAB (p=0.004), with patients aged >59 years representing the largest CRAB group. Clinical diagnosis or manifestation was also significantly associated with CRAB (p=0.005), with respiratory tract infection being the most frequent manifestation. Previous carbapenem use within one month was significantly associated with CRAB (p=0.009), with an odds ratio of 8.33 (95% CI: 1.48–46.94). In contrast, comorbidity was not significantly associated with CRAB (p=0.665). Implications: These findings support strengthening antimicrobial stewardship, particularly the appropriate use and continuous evaluation of carbapenem therapy, alongside enhanced surveillance and infection prevention measures among high-risk patients. Originality: This study provides local epidemiological evidence from an eight-year clinical dataset and integrates demographic, clinical, and previous carbapenem-exposure factors to characterize CRAB at a clinical microbiology laboratory in Jakarta, thereby contributing institution-specific evidence for CRAB surveillance and prevention strategies.
Co-Authors Andi Arus Victor Anis Karuniawati Augustine Natasha Budiman Bela Dimas Seto Prasetyo Dzaki Fadlurrahman Ayyasy Elyn Dohar Idarin Aritonang FITRIYAH SJATHA Gortap Sihotang Hana Paraswati Putri Hindra I. Satari Julie Dewi Barliana Martin Hartiningsih Mubarokah Mubarokah OCTAVIA CHANDRA MUSTIKA Riamin Sitorus Ricky Fernando Santya Fatma Dewi Sari Wiraswasty Tjahjani Mirawati Sudiro Triana Hardianti Gunardi Tubagus M. Kurniadi Victor Sitorus Yulia Rosa Saharman