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All Journal JURNAL TEKNOLOGI LINGKUNGAN JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses
Wulansari, Aida
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Journal : JURNAL BIOLOGI INDONESIA

 1 
VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
INDUKSI TANAMAN POLIPLOID TALAS (COLOCASIA ESCULENTA L.) DENGAN PERLAKUAN ORIZALIN SECARA IN VITRO Wulansari, Aida; Martin, Andri F; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2898

Abstract

ABSTRACTGenetic improvement of taro (Colocasia esculenta L.) is one of important research program to increase productivity and give better cultivation on the marginal land. Induction of polyploid plants is a method useful to increase genetic diversity. The aim of this research was to induce polyploid taro by oryzalin treatment. Polyploidy was induced from in vitro diploid taro ?bentul? using oryzalin at 7.5; 15; 30; 60 and 75 ?M soaked for 3 days. Regenerated shoots were grown on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine. Shoot growth was recorded four weeks (subculture-0), eight weeks (subculture-1) and twelve weeks (subculture-2) after treatments. The results showed that survival rate of treated shoots was 100%. Higher oryzalin concentration reduced the proliferation of shoots, petiole length, numbers of leaves as well as the numbers of roots. Ploidy levels analysis determined by flowcytometer for 122 plantlets were investigated. The results indicated that control shoots were diploid. All treated shoots were polyploids. Oryzalin at 60 ?M gave 50% of tetraploid planlets, 30 ?M of oryzalin gave 5.71% hexaploids, 60 ?M of oryzalin gave 9.09% octaploids. All acclimatized plantlets gave 100% survival rate.Keywords : Colocasia esculenta L., taro, in vitro induced polyploidy, oryzalin
VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
KLASTER PERTUMBUHAN KULTUR TUNAS TALAS (COLOCASIA ESCULENTA (L.) SCHOT.) CV. BENTUL TETRAPLOID BERDASARKAN METODE WARD Wulansari, Aida; Martin, Andri Fadillah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3406

Abstract

ABSTRACTTaro (Colocasia esculenta (L.) Schot.) has been cultivated for a long time in Indonesia. Taro tuber can be used as alternative food for rice substitute to support food diversification program. Taro cv. Bentul is preferable to farmers because it has few buds that are easy to maintain and adaptable in both lowlands and highlands. Its tuber has a delicious taste and a soft texture. Somatic cell manipulation of Indonesian taro through biotechnology may contribute to increase its productivity. One of in vitro technique for somatic cell manipulation is polyploidy. Oryzalin has been able to obtain taro cv. Bentul tetraploid. A total of 17 tetraploid clones were used in this study for clustering. Those clones were obtained from previous research and have stable ploidy level. The objective of this study was to determine cluster Bentul tetraploid of shoot culture using Ward method based on their in vitro growth as an initial selection before further selection in the field. Shoot tips of tetraploid were cultured on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine for 6 weeks. The observed growth variables were number of shoots, length of petiole, number of leaves and roots. The clusterring was done using Ward and Euclidean Distance method followed by Analysis of Varians and Duncan Multiple Range Tests (DMRT). Out of 17 clones observed resulted in 3 clusters. Cluster 1 consisted of 2 clones, cluster 2 consisted of 9 clones, and cluster 3 consisted of 6 clones respectively. The best cluster was cluster 3 which was significantly different on the average number of shoots and leaves. Cluster 3 was dominated by clones derived from oryzalin at 75 ?M.Keywords: taro (Colocasia esculenta (L.) Schot.), tetraploid, in vitro growth, cluster analysis, Ward
Co-Authors Diyah Martanti, Diyah Hafiizh, Erwin Al IMELDA, MARIA laela Sari, laela Martin, Andri F Martin, Andri Fadillah Noorrohmah, Siti Rantau, Deritha Ellfy Sumarnie, Sumarnie Tri Muji Ermayanti Yuyu S Poerba