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All Journal Animal Production : Indonesian Journal of Animal Production Journal of Sustainable Livestock
Haren, Hassan Ishag Hassan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Polymorphism of Myostatin Gene (MSTN) Coding Region in Batur Sheep Haren, Hassan Ishag Hassan; Prayitno, Prayitno; Purwantini, Dattadewi; Sumaryadi, Mas Yedi
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (22.566 KB) | DOI: 10.20884/1.jap.2019.21.1.780

Abstract

The aim of present study was to investigate myostatin gene polymorphism and its association with weaning weight and body weight in Batur sheep, 30 heads of Batur all lambs were fed by concentrated feed. Body weight measured monthly after weaning to the six months of age. DNA Extraction used 200 ul of whole blood samples. To amplify exon 3 region of MSTN gene a specific primer designed using the Primer3 software. The 25 μl volume contained 25 ng of genomic DNA, 12.5 μl 2x Reaction mix of each primer. The cycling protocol was 5 minutes at 95°C as initial denaturation, 35 cycles of denaturing at 94°C for 45 seconds, annealing at 73.9 for 45 seconds, extending at 72°C for 40 s, with final extension at 72°C for 10 minutes. Eleven polymorphic sites were observed in the in 3rd exon region transversions at c.*121 G instead of A, del-T at c.*129, one individual at c.*139 and one individual at c.*158 positions however, one individual sequence disrupted reading frame in whole MSTN sequenced, also weaning weight and 6-month weight of Batur lambs were 21.13±5.70 and 31.64±7.06 to the homozygous whilst 19.99±4.33 and 30.50±5.44 for heterozygote respectively but there is no significe differences seen above all the studied parameters. Homozygous AA lambs had fairly low weaning weight and body weight compared to the heterozygous AB lambs but might have a higher carcass weight, further investigation is needed into the interaction of MSTN with other genes involved in muscle growth.
Isolation of Pathogenic Bacteria Causing Clinical Mastitis and Their Susceptibility to Commercial Antibiotics in Dairy Cows Haren, Hassan Ishag Hassan; Handayanta, Eka; Hilmi, Ihsan; Magistrama, Muhammad Evan
Journal of Sustainable Livestock Vol. 2 No. 1 (2026): Journal of Sustainable Livestock
Publisher : Faculty of Animal Husbandry, Wijayakusuma Purwokerto University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63859/jsl.v2i1.29

Abstract

Clinical mastitis in dairy cattle poses severe economic and health challenges globally, exacerbated by rising antimicrobial resistance (AMR). This systematic review and meta-analysis evaluated the prevalence of mastitis-causing pathogens and their antibiotic resistance profiles from 2010 to 2023. Following PRISMA guidelines, 45 eligible studies were analyzed using a random-effects model. The pooled estimates revealed that Gram-positive bacteria are the primary causative agents, predominantly Staphylococcus intermedius (42.30%) and Staphylococcus aureus (32.97%). Analysis of resistance proportions demonstrated alarming rates against commonly used commercial antibiotics, notably Penicillin (45.2%) and Ampicillin (40.5%). Crucially, methicillin-resistant S. intermedius strains exhibited absolute resistance (100%) to oxacillin and cefoxitin. High inter-study heterogeneity (I² > 75%) was observed, with meta-regression confirming a statistically significant temporal increase in resistance over the past decade. These findings underscore the critical threat of AMR in the dairy sector. The study concludes that there is an urgent necessity for implementing standardized diagnostic procedures, continuous molecular surveillance, and rigorous, evidence-based localized antibiotic stewardship policies to mitigate the spread of resistant pathogens and ensure sustainable dairy production.
Co-Authors Dattadewi Purwantini Eka Handayanta Hilmi, Ihsan M. Y. Sumaryadi Magistrama, Muhammad Evan Prayitno Prayitno