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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Dasar JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Hartati, N Sri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Immunology & microbiology Mathematics

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INDUKSI, MULTIPLIKASI DAN PERTUMBUHAN TUNAS UBI KAYU (MANIHOT ESCULENTA CRANTZ) GENOTIPE UBI KUNING SECARA IN VITRO Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3738

Abstract

ABSTRACTObjectives of the research were to determine the best source of explant origin, the optimum culture media and effects of various concentrations and types of growth regulators on the induction and multiplication as well as the growth of in vitro shoot of cassava Ubi Kuning genotype. The experiment was arranged in a complete randomized design with three factors, i.e the source of explant origin (from field and greenhouse), the growth media containing of 6-Benzyl Amino Purine (BAP) and Gibberellin (GA3) at the concentration level of 1-2 ppm, and multiplication medium supplemented with BAP, Naphtalenic acetic acid (NAA) and Kinetin at concentration level of 0.1 mg/l and 0.5 mg/l. The results showed that the cuttings explants from the field showed more shoots and the average shoot height of axenic culture in the fourth week ranging from 0.2-1.2 cm compared to those from the field (0.5- 1.3 cm). Axenic culture showed no significant growth in growth media (MS) supplemented with BAP and GA3 (1-2 ppm). In the multiplication medium, the highest number of roots were obtained when plantlets were grown in the treatment medium MS + BAP 0.5 mg / l followed by MS + NAA 0.1 mg / l. The largest number of nodes was produced on plantlets grown in MS + BAP media 0.1 mg / l and MS + NAA 0.5 mg / l (p <0.05). While the longest root growth was obtained in plantlets cultured on MS + Kinetin 0.1 mg / l medium. Plantlets showed high survival (100%) after they were acclimatizedfor 4 weeks in a greenhouse using a medium containing a mixture of compost, coco peat, and ground burn husk with a ratio of 1: 1: 1. Keywords: genotype, ubi kayu, ubi kuning  in vitro 
KERAGAMAN UBI KAYU (Manihot esculenta Crantz.) HASIL PERBANYAKAN IN VITRO BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA ISSR Hartanti, Fajri; Miftahudin, Miftahudin; Hartati, N Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1494.884 KB) | DOI: 10.29122/jbbi.v6i2.3055

Abstract

Morphological and Molecular Diversity of Cassava (Manihot esculenta Crantz) Resulted from In Vitro PropagationIn vitro propagation of cassava (Manihot esculenta Crantz) on medium containing plant growth regulator (PGR) may induce morphological variation. This study aimed to analyze the morphological and genetic diversity of 13 genotypes of cassava resulted from in vitro propagation and stem cuttings based on 11 vegetative characters and 7 ISSR markers. Morphological and genetic characters were scored and used for clustering using NTSYS-pc 2.11a. Roti control and Adira 4 control genotypes that were in vitro propagated without PGR addition showed different morphological characters with Roti variant and FEC-25 genotypes that were in vitro propagated with the addition of PGR. Morphological and molecular characters of 13 genotypes showed high diversity. Clustering analysis based on morphological characters classified the in vitro propagated and control plants into four groups at 45.6% similarity. Clustering analysis based on molecular characters classified the plants into three groups at 66.0% similarity.Keywords: cassava; diversity; in vitro; ISSR; morphologyABSTRAKPerbanyakan tanaman ubi kayu (Manihot esculenta Crantz) secara in vitro menggunakan zat pengatur tumbuh (ZPT) diyakini dapat menginduksi variasi morfologi. Tujuan dari penelitian ini adalah untuk menganalisis keragaman morfologi dan molekuler dari 13 genotipe ubi kayu hasil perbanyakan in vitro dan perbanyakan dengan stek batang berdasarkan 11 karakter vegetatif dan 7 penanda ISSR. Karakter morfologi dan molekuler diskor untuk analisis kelompok menggunakan program NTSYS-pc 2.11a. Genotipe Roti kontrol dan Adira 4 kontrol yang merupakan hasil perbanyakan in vitro tanpa penambahan ZPT menunjukkan perbedaan variasi morfologi dengan genotipe Roti varian dan FEC-25 yang merupakan tanaman hasil perbanyakan dengan penambahan ZPT. Hasil analisis pada 13 genotipe menunjukkan adanya keragaman yang tinggi. Hasil analisis kelompok berdasarkan penanda morfologi memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 4 kelompok dengan nilai koefisen similaritas 45,6%. Hasil analisis kelompok berdasarkan penanda molekuler memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 3 kelompok dengan nilai koefisen similaritas 66,0%.
Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro Rahman, Nurhamidar; Supatmi, Supatmi; Fitriani, Hani; Hartati, N Sri
Jurnal ILMU DASAR Vol 21 No 2 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v21i2.9307

Abstract

In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation
A Comparative Study of the Organellar Genome of Gyrinops versteegii and Aquilaria malaccensis Hartati, Hartati; Cartealy, Imam Civi; Supatmi, Supatmi; Rahmawati, Syamsidah; Hartati, N Sri; Siregar, Ulfah Juniarti; Siregar, Iskandar Zulkarnaen
Jurnal Manajemen Hutan Tropika Vol. 30 No. 3 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.30.3.326

Abstract

Gyrinops versteegii and Aquilaria malaccensis are two important species of the Aquilarieae tribe. The main problem of this tribe is the challenge of species identification that is strongly dependent on the presence of flowers and fruit, which are not always available. The availability of whole genome information is expected to address the problems of species identification. This research aims to construct and compare the chloroplast and mitochondrial genomes of G. versteegii and A. malaccensis from short-read data using the NOVOplasty and GetOrganelle assembler. The chloroplast genome assembly revealed a full-length quadripartite circular structure with sizes of 174.814 bp (G. versteegii) and 174.821–174.822 bp (A. malaccensis), with highly conserved gene and organization. Meanwhile, the mitochondrial genome is multipartite with a size of 400.012 bp (G. versteegii) and 400.000 bp (A. malaccensis), with highly variable genes and organization due to the presence of gene cluster repeats. The LSC/IR/SCC region borders and phylogenetic analysis in chloroplasts indicate variations between the genomes of these two species. The investigation of nucleotide diversity in the chloroplast genome revealed that the trnL-rpl32 region had the highest nucleotide diversity (Pi = 0.03). This information will be useful in the future for a variety of downstream analyses.
Co-Authors Cartealy, Imam Civi Hani Fitriani, Hani Hartanti, Fajri Hartati Hartati ISKANDAR ZULKARNAEN SIREGAR Miftahudin . Rahman, Nurhamidar Supatmi Supatmi Syamsidah Rahmawati, Syamsidah Ulfah Juniarti Siregar