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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu Dasar JURNAL BIOLOGI INDONESIA Biofarm : Jurnal Ilmiah Pertanian
Rahman, Nurhamidar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Mathematics

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INDUKSI, MULTIPLIKASI DAN PERTUMBUHAN TUNAS UBI KAYU (MANIHOT ESCULENTA CRANTZ) GENOTIPE UBI KUNING SECARA IN VITRO Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3738

Abstract

ABSTRACTObjectives of the research were to determine the best source of explant origin, the optimum culture media and effects of various concentrations and types of growth regulators on the induction and multiplication as well as the growth of in vitro shoot of cassava Ubi Kuning genotype. The experiment was arranged in a complete randomized design with three factors, i.e the source of explant origin (from field and greenhouse), the growth media containing of 6-Benzyl Amino Purine (BAP) and Gibberellin (GA3) at the concentration level of 1-2 ppm, and multiplication medium supplemented with BAP, Naphtalenic acetic acid (NAA) and Kinetin at concentration level of 0.1 mg/l and 0.5 mg/l. The results showed that the cuttings explants from the field showed more shoots and the average shoot height of axenic culture in the fourth week ranging from 0.2-1.2 cm compared to those from the field (0.5- 1.3 cm). Axenic culture showed no significant growth in growth media (MS) supplemented with BAP and GA3 (1-2 ppm). In the multiplication medium, the highest number of roots were obtained when plantlets were grown in the treatment medium MS + BAP 0.5 mg / l followed by MS + NAA 0.1 mg / l. The largest number of nodes was produced on plantlets grown in MS + BAP media 0.1 mg / l and MS + NAA 0.5 mg / l (p <0.05). While the longest root growth was obtained in plantlets cultured on MS + Kinetin 0.1 mg / l medium. Plantlets showed high survival (100%) after they were acclimatizedfor 4 weeks in a greenhouse using a medium containing a mixture of compost, coco peat, and ground burn husk with a ratio of 1: 1: 1. Keywords: genotype, ubi kayu, ubi kuning  in vitro 
The Influence of Various Growth Regulators on Induction Organogenic Callus from Gajah and Kuning Cassava Genotype (Manihot esculenta Crantz) Rahman, Nurhamidar; Fitriani, Hani; Rahman, Nurhaidar; Hartati, N. Sri
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.9305

Abstract

Kuning and Gajah genotypes are two collections of cassava in the Biotechnology Research Center for Germplasm, LIPI with the advantages of each genotype are high beta carotene and high production. The multiplication in in vitro culture can be done one of them through organogenesis. The aim of this study was to evaluate the effect of using 2,4-D; NAA and Kinetin are used singly for the formation of organogenesis of cassava in the Kuning Cassava and Gajah genotypes. This research was conducted at the Laboratory of Molecular Genetics and Modification of Plant Biosynthetic Pathways, Biteknologi Research Center, LIPI, Bogor since January - February 2018. The source of explants were young leaves and petiols from cassava plant culture in vitro genotypes of Gajah and Kuning yam which were three months old. in culture. The basic media used as a planting medium were Murashige and Skoog (MS) media with the addition of growth regulators (ZPT) singly, 2,4-D, NAA and Kinetin with two concentrations of ZPT each, 8 and 10 mg L- 1 This research was arranged based on a completely randomized design factorial pattern consisting of 2 factors. All data obtained were analyzed using ANOVA and if there is an influence then proceed with the DMRT test with an error rate of 5% using the SPSS program. The highest number of Kuning genotype cassava organogenic callus that developed into shoots on the medium added by ZPT was 2.4 D and kinetin with the same concentration of 8 mg L-1. Formation of the best organogenic callus in petiol explants in the media with the addition of a single 2,4-D and Kinetin with the same concentration of 8 mg L-1. Keywords: Cassava, growth regulators, organogenic.
Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro Rahman, Nurhamidar; Supatmi, Supatmi; Fitriani, Hani; Hartati, N Sri
Jurnal ILMU DASAR Vol 21 No 2 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v21i2.9307

Abstract

In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation
Tissue Culture Initiation Using Local Maize Explants from the Field Ningsih, Irnawati; Wahyuni, Febriana Dwi; Rahman, Nurhamidar; Fitriani, Hani; Rianawati, Sri; Supriadi, Dedi; Mulyaningsih, Enung Sri
Biofarm : Jurnal Ilmiah Pertanian Vol. 20 No. 2 (2024): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v20i2.5019

Abstract

Inisiasi kultur jaringan adalah salah satu metode bioteknologi yang bertujuan untuk menghasilkan tanaman baru menggunakan eksplan yang telah ada. Teknik kultur jaringan saat ini telah berkembang perannya sebagai tool untuk menghasilkan tanaman hasil pemuliaan modern seperti hasil rekayasa. Namun untuk dapat dimanfaatkan sebagai tool, penguasaan kultur jaringan di tahap awal (inisiasi) perlu dikuasai. Jagung lokal yang berasal dari lapangan merupakan salah satu eksplan yang dapat dimanfaatkan untuk kegiatan inisiasi kultur jaringan. Hal ini menarik dilakukan karena jagung merupakan tanaman graminea yang paling rekalsitran terhadap kultur jaringan. Kegiatan dimulai dengan memilih varietas jagung lokal, menggunakan material genetik berupa benih tua (jagung pipilan) kemudian dilakukanlah optimasi proses sterilisasi. Teknik sterilisasi yang digunakan bersifat fast dan overnight. Benih yang telah steril ditumbuhkan pada media dasar. Selanjutnya untuk percobaan kultur jaringan, material genetik dibedakan atas bagian: buku utuh, buku dibelah dan ujung akar. Material eksplan diperoleh dari seedling yang tumbuh steril dan normal pada hasil percobaan sterilisasi. Eksplan yang diperoleh di tanam pada media NB, 1⁄2 MS, dan SH yang mengandung kombinasi zat pengatur tumbuh (ZPT) yaitu: label A) TDZ 0,75 mg/L (Thidiazuron) & BAP 0,25 mg/L (Benzyl amino purin), label B) dengan hormon NAA 0,5 mg/L (Naptalaneacetic acid) & BAP 1 mg/L (Benzyl amino purin), dan label C) dengan hormon 2,4-Dichlorophenoxy Acid 1 mg/L dan Kinetin 0,5 mg/L yang dilengkapi asam amino (proline, casein) sumber karbon (sukrosa), dan pemadat (phytagel). Hasil penelitian ini didapatkan bahwa dengan sterilisasi cepat, pertumbuhan tanaman memiliki hasil perkembangan yang cepat yaitu tumbuh pada hari ke 10, namun memiliki tingkat kontaminasi yang tinggi. Sedangkan dengan sterilisasi overnight memiliki hasil perkembangan yang lambat yaitu tumbuh pada hari ke 16, namun memiliki tingkat kontaminasi yang rendah. Kombinasi ZPT dengan media yang paling cocok adalah media NB yang dikombinasikan dengan ZPT TDZ 0,75 mg/L dan BAP 0,25 mg/L, menghasilkan plantlet dari bagian buku, dan kalus dari bagian ujung akar, walaupun kalus yang di maksud masih kalus secara umum. Penyimpanan pada lingkungan gelap, memiliki persentase pertumbuhan yang lebih cepat jika dibandingkan dengan perlakuan penyimpanan pada lingkungan terang. Dan hasil akhir juga menunjukan bahwa pada kondisi terang, tingkat dorman/ mati nya eksplan lebih tinggi.
Karakteristik kimia dan keamanan mikroba tepung ikan teri hitam (Stolephorus commersonii): Chemical characteristics and microbial safety of commerson’s anchovy meal (Stolephorus commersonnii) Litaay, Christina; Indriati, Ashri; Andriansyah, Raden Cecep Erwan; Novianti, Fithria; Purwandoko, Pradeka Brilyan; Rahman, Nurhaidar; Nuraini, Laela; Rahman, Nurhamidar; Hidayat, Taufik
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 26 No. 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.48355

Abstract

Ikan teri hitam (Stolephorus commersonii) merupakan jenis ikan pelagis kecil yang bernilai gizi tinggi. Ikan teri hitam memiliki nilai ekonomi yang tinggi, namun pemanfaatannya masih bersifat tradisional. Peningkatan nilai tambah ikan teri hitam dapat dilakukan melalui diversifikasi produk dalam bentuk tepung ikan. Penelitian ini bertujuan untuk menentukan karakteristik tepung ikan teri hitam berdasarkan komposisi kimia, kandungan mineral, dan cemaran mikroba. Metode penelitian yang digunakan adalah metode eksperimen pengolahan tepung ikan menggunakan modifikasi media perendaman air pada suhu 25oC, selama 15 menit. Parameter yang diuji yaitu analisis proksimat, mineral (besi, magnesium, dan kalsium), dan cemaran mikroba (Angka Lempeng Total (ALT), kapang khamir, dan Escherichia coli). Hasil analisis menunjukkan bahwa tepung ikan teri mengandung kadar protein 70,16%, air 9,62%, abu 14,85%, dan lemak 4,55%. Kadar besi (5,99 mg/g), magnesium (163,565 mg/g), dan kalsium (6179,95 mg/g). Cemaran mikroba Angka Lempeng Total (ALT) sebesar 1,15 koloni/g, kapang khamir <10 koloni/g, dan Escherichia coli negatif.
Co-Authors Andriansyah, Raden Cecep Erwan Christina Litaay Dedi Supriadi Enung Sri Mulyaningsih Febriana Dwi Wahyuni Hani Fitriani, Hani Hartati, N Sri Indriati, Ashri N. Sri Hartati, N. Sri Ningsih, Irnawati Novianti, Fithria Nuraini, Laela Purwandoko, Pradeka Brilyan Rahman, Nurhaidar Sri Rianawati Supatmi Supatmi Taufik Hidayat