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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu Dasar JURNAL BIOLOGI INDONESIA Biofarm : Jurnal Ilmiah Pertanian Proceeding Biology Education Conference Annales Bogorienses
Rahman, Nurhamidar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health

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INDUKSI, MULTIPLIKASI DAN PERTUMBUHAN TUNAS UBI KAYU (MANIHOT ESCULENTA CRANTZ) GENOTIPE UBI KUNING SECARA IN VITRO Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3738

Abstract

ABSTRACTObjectives of the research were to determine the best source of explant origin, the optimum culture media and effects of various concentrations and types of growth regulators on the induction and multiplication as well as the growth of in vitro shoot of cassava Ubi Kuning genotype. The experiment was arranged in a complete randomized design with three factors, i.e the source of explant origin (from field and greenhouse), the growth media containing of 6-Benzyl Amino Purine (BAP) and Gibberellin (GA3) at the concentration level of 1-2 ppm, and multiplication medium supplemented with BAP, Naphtalenic acetic acid (NAA) and Kinetin at concentration level of 0.1 mg/l and 0.5 mg/l. The results showed that the cuttings explants from the field showed more shoots and the average shoot height of axenic culture in the fourth week ranging from 0.2-1.2 cm compared to those from the field (0.5- 1.3 cm). Axenic culture showed no significant growth in growth media (MS) supplemented with BAP and GA3 (1-2 ppm). In the multiplication medium, the highest number of roots were obtained when plantlets were grown in the treatment medium MS + BAP 0.5 mg / l followed by MS + NAA 0.1 mg / l. The largest number of nodes was produced on plantlets grown in MS + BAP media 0.1 mg / l and MS + NAA 0.5 mg / l (p <0.05). While the longest root growth was obtained in plantlets cultured on MS + Kinetin 0.1 mg / l medium. Plantlets showed high survival (100%) after they were acclimatizedfor 4 weeks in a greenhouse using a medium containing a mixture of compost, coco peat, and ground burn husk with a ratio of 1: 1: 1. Keywords: genotype, ubi kayu, ubi kuning  in vitro 
The Influence of Various Growth Regulators on Induction Organogenic Callus from Gajah and Kuning Cassava Genotype (Manihot esculenta Crantz) Rahman, Nurhamidar; Fitriani, Hani; Rahman, Nurhaidar; Hartati, N. Sri
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.9305

Abstract

Kuning and Gajah genotypes are two collections of cassava in the Biotechnology Research Center for Germplasm, LIPI with the advantages of each genotype are high beta carotene and high production. The multiplication in in vitro culture can be done one of them through organogenesis. The aim of this study was to evaluate the effect of using 2,4-D; NAA and Kinetin are used singly for the formation of organogenesis of cassava in the Kuning Cassava and Gajah genotypes. This research was conducted at the Laboratory of Molecular Genetics and Modification of Plant Biosynthetic Pathways, Biteknologi Research Center, LIPI, Bogor since January - February 2018. The source of explants were young leaves and petiols from cassava plant culture in vitro genotypes of Gajah and Kuning yam which were three months old. in culture. The basic media used as a planting medium were Murashige and Skoog (MS) media with the addition of growth regulators (ZPT) singly, 2,4-D, NAA and Kinetin with two concentrations of ZPT each, 8 and 10 mg L- 1 This research was arranged based on a completely randomized design factorial pattern consisting of 2 factors. All data obtained were analyzed using ANOVA and if there is an influence then proceed with the DMRT test with an error rate of 5% using the SPSS program. The highest number of Kuning genotype cassava organogenic callus that developed into shoots on the medium added by ZPT was 2.4 D and kinetin with the same concentration of 8 mg L-1. Formation of the best organogenic callus in petiol explants in the media with the addition of a single 2,4-D and Kinetin with the same concentration of 8 mg L-1. Keywords: Cassava, growth regulators, organogenic.
Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro Rahman, Nurhamidar; Supatmi, Supatmi; Fitriani, Hani; Hartati, N Sri
Jurnal ILMU DASAR Vol 21 No 2 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v21i2.9307

Abstract

In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation
Tissue Culture Initiation Using Local Maize Explants from the Field Ningsih, Irnawati; Wahyuni, Febriana Dwi; Rahman, Nurhamidar; Fitriani, Hani; Rianawati, Sri; Supriadi, Dedi; Mulyaningsih, Enung Sri
Biofarm : Jurnal Ilmiah Pertanian Vol. 20 No. 2 (2024): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v20i2.5019

Abstract

Inisiasi kultur jaringan adalah salah satu metode bioteknologi yang bertujuan untuk menghasilkan tanaman baru menggunakan eksplan yang telah ada. Teknik kultur jaringan saat ini telah berkembang perannya sebagai tool untuk menghasilkan tanaman hasil pemuliaan modern seperti hasil rekayasa. Namun untuk dapat dimanfaatkan sebagai tool, penguasaan kultur jaringan di tahap awal (inisiasi) perlu dikuasai. Jagung lokal yang berasal dari lapangan merupakan salah satu eksplan yang dapat dimanfaatkan untuk kegiatan inisiasi kultur jaringan. Hal ini menarik dilakukan karena jagung merupakan tanaman graminea yang paling rekalsitran terhadap kultur jaringan. Kegiatan dimulai dengan memilih varietas jagung lokal, menggunakan material genetik berupa benih tua (jagung pipilan) kemudian dilakukanlah optimasi proses sterilisasi. Teknik sterilisasi yang digunakan bersifat fast dan overnight. Benih yang telah steril ditumbuhkan pada media dasar. Selanjutnya untuk percobaan kultur jaringan, material genetik dibedakan atas bagian: buku utuh, buku dibelah dan ujung akar. Material eksplan diperoleh dari seedling yang tumbuh steril dan normal pada hasil percobaan sterilisasi. Eksplan yang diperoleh di tanam pada media NB, 1⁄2 MS, dan SH yang mengandung kombinasi zat pengatur tumbuh (ZPT) yaitu: label A) TDZ 0,75 mg/L (Thidiazuron) & BAP 0,25 mg/L (Benzyl amino purin), label B) dengan hormon NAA 0,5 mg/L (Naptalaneacetic acid) & BAP 1 mg/L (Benzyl amino purin), dan label C) dengan hormon 2,4-Dichlorophenoxy Acid 1 mg/L dan Kinetin 0,5 mg/L yang dilengkapi asam amino (proline, casein) sumber karbon (sukrosa), dan pemadat (phytagel). Hasil penelitian ini didapatkan bahwa dengan sterilisasi cepat, pertumbuhan tanaman memiliki hasil perkembangan yang cepat yaitu tumbuh pada hari ke 10, namun memiliki tingkat kontaminasi yang tinggi. Sedangkan dengan sterilisasi overnight memiliki hasil perkembangan yang lambat yaitu tumbuh pada hari ke 16, namun memiliki tingkat kontaminasi yang rendah. Kombinasi ZPT dengan media yang paling cocok adalah media NB yang dikombinasikan dengan ZPT TDZ 0,75 mg/L dan BAP 0,25 mg/L, menghasilkan plantlet dari bagian buku, dan kalus dari bagian ujung akar, walaupun kalus yang di maksud masih kalus secara umum. Penyimpanan pada lingkungan gelap, memiliki persentase pertumbuhan yang lebih cepat jika dibandingkan dengan perlakuan penyimpanan pada lingkungan terang. Dan hasil akhir juga menunjukan bahwa pada kondisi terang, tingkat dorman/ mati nya eksplan lebih tinggi.
Karakteristik kimia dan keamanan mikroba tepung ikan teri hitam (Stolephorus commersonii): Chemical characteristics and microbial safety of commerson’s anchovy meal (Stolephorus commersonnii) Litaay, Christina; Indriati, Ashri; Andriansyah, Raden Cecep Erwan; Novianti, Fithria; Purwandoko, Pradeka Brilyan; Rahman, Nurhaidar; Nuraini, Laela; Rahman, Nurhamidar; Hidayat, Taufik
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 26 No. 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.48355

Abstract

Ikan teri hitam (Stolephorus commersonii) merupakan jenis ikan pelagis kecil yang bernilai gizi tinggi. Ikan teri hitam memiliki nilai ekonomi yang tinggi, namun pemanfaatannya masih bersifat tradisional. Peningkatan nilai tambah ikan teri hitam dapat dilakukan melalui diversifikasi produk dalam bentuk tepung ikan. Penelitian ini bertujuan untuk menentukan karakteristik tepung ikan teri hitam berdasarkan komposisi kimia, kandungan mineral, dan cemaran mikroba. Metode penelitian yang digunakan adalah metode eksperimen pengolahan tepung ikan menggunakan modifikasi media perendaman air pada suhu 25oC, selama 15 menit. Parameter yang diuji yaitu analisis proksimat, mineral (besi, magnesium, dan kalsium), dan cemaran mikroba (Angka Lempeng Total (ALT), kapang khamir, dan Escherichia coli). Hasil analisis menunjukkan bahwa tepung ikan teri mengandung kadar protein 70,16%, air 9,62%, abu 14,85%, dan lemak 4,55%. Kadar besi (5,99 mg/g), magnesium (163,565 mg/g), dan kalsium (6179,95 mg/g). Cemaran mikroba Angka Lempeng Total (ALT) sebesar 1,15 koloni/g, kapang khamir <10 koloni/g, dan Escherichia coli negatif.
Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N. Sri
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.
Massive In Vitro Propagation of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi; Ardiyanti, Nurdiya; Rahman, Nurhamidar; Sudarmonowati, Enny
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its essential oil contents used for important essence of perfume in the perfumery industry. However, its population has significantly depleted since the planting materials of this tree using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves in length of 1-3 and 4-7 mm cultured in MS medium containing 0.5 mg/l and 1 mg/l indole acetic acid (IAA), MS medium supplemented with 1 mg/l IAA and 0.2 mg/l kinetin and half concentration of MS medium supplemented with 1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when it repetitively transferred into MS medium supplemented with 1.7 mg/l BAP, 1 mg/l proline or 1.5 mg/l BAP and 1.2 mg/l kinetin every 3 weeks. The FEC shows its optimum maturation and regeneration in the MS medium supplemented with 1.5 mg/l BAP and 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets were perfectly conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant namely Murraya paniculata, (L) Jack which gave the best percentage of survival rate and the lowest percentage of fallen leaves. These findings may improve the massive propagation of sandalwood through FEC as well as a useful material for further genetic improvement of sandalwood by using FEC as material for genetic transformation.
Daya Hidup dan Pertumbuhan Kultur In Vitro Ubi Kayu (Manihot esculanta) Genotip Ubi Kuning Hasil Radiasi pada Media MS Tanpa Zat Pengatur Tumbuh Rahman, Nurhamidar
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 20, No 1 (2023): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Ubi Kayu kuning juga dikenal   dikenal sebagai ubi kayu manis atau sweet cassava (Manihot esculanta), adalah genotip ubi kayu yang memiliki warna umbi yang khas, yaitu kuning. Ubi kayu kuning juga memilki nilai ekonomi yang penting. Selain digunakan sebagai sumber pangan, umbi ubi kayu kuning juga digunakan dalam industri makanan untuk menghasilkan tepung ubi kayu yang dapat digunakan dalam pembuatan roti, dan kue. Selain itu ubi kayu kuning juga dapat digunakan dalam industri olahan, seperti bioetanol dan bahan baku industri kimia. Pembudidayaan ubi kayu melalui teknik in vitro memberikan peluang untuk melakukan perbanyakan secara massal. Perbanyakan secara in vitro yang akan dipadukan dengan teknik lain seperti radiasi akan bermanfaat untuk menunjang kegiatan penelitian perbaikan tanaman. Keragaman genetik pada tanaman dapat ditingkatkan dengan mutasi menggunakan radiasi sinar gamma dari Cobalt 60 (Co60). Tujuan penelitian ini adalah mempelajari pertumbuhan ubi kuning hasil radiasi dengan dua dosis radiasi (5 gray dan 30 gray).Rancangan percobaan yang digunakan dalam penelitian ini adalah rancangan acak lengkap sederhana (RAL) dengan satu faktor perlakuan yaitu sinar gamma dari Cobalt-60 (Co60). Dosis radiasi yang digunakan adalah 0, 5, dan 30 Gray (Gy). Kemudian dilakukan sub kultur setelah penanaman selama 60 hari, tanaman yang masih hidup yaitu pada dosis 5 gray dan 30 gray. Hasil penelitian yang diperoleh menunjukkan plantlet hasil radiasi sinar gamma dengan dosis 5 gray pertumbuhannya terhambat yang terlihat dari tinggi tanaman (2 - 2.3 cm) yang nilainya lebih rendah dibanding dengan kontrol (2.5 -.2.7 cm).  Pada dosis 30 gray beberapa tanaman pertumbuhannya terhambat, tetapi tidak terlalu jauh jika dibandingkan kontrol.  Pada dosis 30 gray beberapa tanaman pada beberapa kali periode sub kultur menggunakan media MS tanpa zat pengatur tumbuh. Sub kultur pada media MS dilakukan setelah tunas ubi kayu hasil radiasi radiasi berumur 60 hari dengan periode sub kultur setiap 2 minggu yang dilakukan sebanyak 2 kali. Jadi dapat disimpulkan bahwa Parameter pertumbuhan yang diamati meliputi tinggi tanaman, jumlah daun dan jumlah akar dari plantlet hasil radiasi sinar gamma dengan dosis 5  gray  pada media MS tanpa zat pengatur tumbuh  terlihat dari pertumbuhannya  terhambat,  tetapi tidak  terlalu  jauh  jika  dibandingkan  kontrol. Hasil  penelitian  yang  telah  diperoleh diharapkan  dapat  dimanfaatkan  untuk mendapatkan  kandidat  ubi  kayu  unggul  baik daya hasilnya maupun kandungan nutrisinya.
Co-Authors Andriansyah, Raden Cecep Erwan Ardiyanti, Nurdiya Christina Litaay Dedi Supriadi Enny Sudarmonowati Enung Sri Mulyaningsih Febriana Dwi Wahyuni Hani Fitriani, Hani Hartati, N Sri Indriati, Ashri N. Sri Hartati, N. Sri Ningsih, Irnawati Novianti, Fithria Nuraini, Laela Purwandoko, Pradeka Brilyan Rahman, Nurhaidar Sri Rianawati Supatmi Supatmi Taufik Hidayat