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The Influence of Various Growth Regulators on Induction Organogenic Callus from Gajah and Kuning Cassava Genotype (Manihot esculenta Crantz) Rahman, Nurhamidar; Fitriani, Hani; Rahman, Nurhaidar; Hartati, N. Sri
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.9305

Abstract

Kuning and Gajah genotypes are two collections of cassava in the Biotechnology Research Center for Germplasm, LIPI with the advantages of each genotype are high beta carotene and high production. The multiplication in in vitro culture can be done one of them through organogenesis. The aim of this study was to evaluate the effect of using 2,4-D; NAA and Kinetin are used singly for the formation of organogenesis of cassava in the Kuning Cassava and Gajah genotypes. This research was conducted at the Laboratory of Molecular Genetics and Modification of Plant Biosynthetic Pathways, Biteknologi Research Center, LIPI, Bogor since January - February 2018. The source of explants were young leaves and petiols from cassava plant culture in vitro genotypes of Gajah and Kuning yam which were three months old. in culture. The basic media used as a planting medium were Murashige and Skoog (MS) media with the addition of growth regulators (ZPT) singly, 2,4-D, NAA and Kinetin with two concentrations of ZPT each, 8 and 10 mg L- 1 This research was arranged based on a completely randomized design factorial pattern consisting of 2 factors. All data obtained were analyzed using ANOVA and if there is an influence then proceed with the DMRT test with an error rate of 5% using the SPSS program. The highest number of Kuning genotype cassava organogenic callus that developed into shoots on the medium added by ZPT was 2.4 D and kinetin with the same concentration of 8 mg L-1. Formation of the best organogenic callus in petiol explants in the media with the addition of a single 2,4-D and Kinetin with the same concentration of 8 mg L-1. Keywords: Cassava, growth regulators, organogenic.
Morphological Variation and Beta Carotene Contents of Several Clones of Ubi Kuning Cassava Genotype Derived from Irradiated Shoot in vitro Rahman, Nurhamidar; Supatmi, Supatmi; Fitriani, Hani; Hartati, N Sri
Jurnal ILMU DASAR Vol 21 No 2 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v21i2.9307

Abstract

In line with the increase in cassava production and the development of nutrient-rich cassava in order to support national food diversification and biofortification programs, the selection of selected varieties of cassava varieties, which are superior in nutrients especially rich in beta carotene is very necessary. Beta carotene is an important source of antioxidants to scavange free radicals and is a provitamin A precusor to form vitamin A. The development of superior cassava riching in beta carotene can be done through the mutation approach with gamma irradiation. The observation of changes in morphological characters and levels of beta carotene from irradiated cassava need to be done to get the superior beta carotene cassava clone candidates, which could be developed in the future. This experiment was conducted at the Biotechnology Research Center, LIPI. The sample used in this study originated from in vitro shoots from several Ubi Kuning clones resulting from 10 Gy radiation, which were then transferred to the field. Observations of morphological characters and levels of beta carotene of Ubi Kuning were carried out in the third generation, which was harvested at the age of 10 months. The morphological analysis of irradiated Ubi Kuning showed that there was a difference in the intensity of tuber color between some of irradiated Ubi Kuning clones compared to the control. The determination of beta carotene levels based on the standard beta carotene curve found that the highest content of beta carotene was found in the UK Rad 3.4 clone with beta carotene content of 0.252 μg / mL compared to the control (0.219 μg / mL). The lowest beta carotene content was obtained in UK Rad 3.3 (0.048 μg / mL), followed by UK Rad 3.2 (0.221 μg / mL) and UK Rad 4.1 (0.120 μg / mL). This shows that the irradiated Ubi Kuning at dosage of 10 Gy caused variations in the intensity of tuber colors and the content of beta carotene from the cassava.Keywords: Ubi Kuning, beta carotene, Gamma light irradiation
Evaluation of Adaptation and Production of Three Selected Cassava (Manihot esculenta Crantz) in Peat Land Area of Central Kalimantan Fitriani, Hani; Hartati, N. Sri; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 20 No 2 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.553 KB) | DOI: 10.19184/jid.v20i2.7449

Abstract

Along with the increasing the of the land-use change of the fertile agricultural land in Java, the better awareness has been subjected to the importance of function peat land for agricultural development. Cassava (Manihot esculenta Crantz) is a tuber plant that potentially developed as important carbohydrate source. The objective of this research was to identify superior cassava variety candidate of selected cassava developed by of Research Centre Biotechnology, LIPI which has high adaptation capability and yield in Central Kalimantan peat land area. This research was conducted from July 2010 through May 2011 in Central Kalimantan Province (Kalampangan dan Pulang Pisau in total of area 1.5 and 2.25 ha respectively). There were three genotypes of cassava tested, i.e. Iding (high amylose), Gebang (low amylose), and Menti (high starch) compared to Adira 4 (35 ton/ha) and Darul Hidayah (102.1 ton/ha). The variables observed were plant height (cm) and tuber weight (grams). Observations were conducted at the age of 10 months. Data was analyzed using SPSS version 16.0. The results showed that the plant height and yield were higher in Pulang Pisau than Kalampangan even though the difference is not significant (P ≤ 0,05). Darul Hidayah has highest plant height and yield, 229.8 cm and 2271 g respectively among other genotypes/varieties grown at Pulang Pisau even though the value was not significant, whereas in Kalampangan, Darul Hidayah showed the lowest yield (670 g). Based on the data obtained, the Darul Hidayah variety can be cultivated by farmers in Pulang Pisau peat land to fulfill the availability of superior cassava seedlings for food, feed and industry. Keywords: Peat, tuber yield, plant height, liquid organic fertilizer, yam wood.
Tissue Culture Initiation Using Local Maize Explants from the Field Ningsih, Irnawati; Wahyuni, Febriana Dwi; Rahman, Nurhamidar; Fitriani, Hani; Rianawati, Sri; Supriadi, Dedi; Mulyaningsih, Enung Sri
Biofarm : Jurnal Ilmiah Pertanian Vol. 20 No. 2 (2024): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v20i2.5019

Abstract

Inisiasi kultur jaringan adalah salah satu metode bioteknologi yang bertujuan untuk menghasilkan tanaman baru menggunakan eksplan yang telah ada. Teknik kultur jaringan saat ini telah berkembang perannya sebagai tool untuk menghasilkan tanaman hasil pemuliaan modern seperti hasil rekayasa. Namun untuk dapat dimanfaatkan sebagai tool, penguasaan kultur jaringan di tahap awal (inisiasi) perlu dikuasai. Jagung lokal yang berasal dari lapangan merupakan salah satu eksplan yang dapat dimanfaatkan untuk kegiatan inisiasi kultur jaringan. Hal ini menarik dilakukan karena jagung merupakan tanaman graminea yang paling rekalsitran terhadap kultur jaringan. Kegiatan dimulai dengan memilih varietas jagung lokal, menggunakan material genetik berupa benih tua (jagung pipilan) kemudian dilakukanlah optimasi proses sterilisasi. Teknik sterilisasi yang digunakan bersifat fast dan overnight. Benih yang telah steril ditumbuhkan pada media dasar. Selanjutnya untuk percobaan kultur jaringan, material genetik dibedakan atas bagian: buku utuh, buku dibelah dan ujung akar. Material eksplan diperoleh dari seedling yang tumbuh steril dan normal pada hasil percobaan sterilisasi. Eksplan yang diperoleh di tanam pada media NB, 1⁄2 MS, dan SH yang mengandung kombinasi zat pengatur tumbuh (ZPT) yaitu: label A) TDZ 0,75 mg/L (Thidiazuron) & BAP 0,25 mg/L (Benzyl amino purin), label B) dengan hormon NAA 0,5 mg/L (Naptalaneacetic acid) & BAP 1 mg/L (Benzyl amino purin), dan label C) dengan hormon 2,4-Dichlorophenoxy Acid 1 mg/L dan Kinetin 0,5 mg/L yang dilengkapi asam amino (proline, casein) sumber karbon (sukrosa), dan pemadat (phytagel). Hasil penelitian ini didapatkan bahwa dengan sterilisasi cepat, pertumbuhan tanaman memiliki hasil perkembangan yang cepat yaitu tumbuh pada hari ke 10, namun memiliki tingkat kontaminasi yang tinggi. Sedangkan dengan sterilisasi overnight memiliki hasil perkembangan yang lambat yaitu tumbuh pada hari ke 16, namun memiliki tingkat kontaminasi yang rendah. Kombinasi ZPT dengan media yang paling cocok adalah media NB yang dikombinasikan dengan ZPT TDZ 0,75 mg/L dan BAP 0,25 mg/L, menghasilkan plantlet dari bagian buku, dan kalus dari bagian ujung akar, walaupun kalus yang di maksud masih kalus secara umum. Penyimpanan pada lingkungan gelap, memiliki persentase pertumbuhan yang lebih cepat jika dibandingkan dengan perlakuan penyimpanan pada lingkungan terang. Dan hasil akhir juga menunjukan bahwa pada kondisi terang, tingkat dorman/ mati nya eksplan lebih tinggi.
KONFLIK WANITA KARIR DENGAN BEBAN KERJA GANDA (DOUBLE BARDEN) Fitriani, Hani; Susanti, Liana Dewi; Astuti, Rahma Yudi
Jurnal Studi Gender dan Anak Vol 2 No 01 (2020): SETARA: Jurnal Studi Gender dan Anak
Publisher : Center of Gender Studies and Child of State Islamic Institute of Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32332/jsga.v2i01.2074

Abstract

The participation of women at this time rather than just demanding equal rights but also expresses its function has the meaning for the development of society Indonesia. conflict of dual role is the simultaneous occurrence of two or more role allotment, which is the fulfillment of a role that will give rise to difficulties in fulfilling the role of the other. Work-family conflict has two components, namely family affairs interfere with work, work-family conflict can arise due to the Affairs of the jobs came family. Conflicts arising as a result of household chores and all the consequences disrupt the performance of the functions of the employment of mothers in the workplace. An adaptive response, linked by characteristics and individual psychology or process which is a consecuetion of any external action, situation or event that puts the demands of special psychological and or physical person. The results of the work can be accomplished by a person or group of people in an organization in accordance with the authority and responsibility of their respective efforts in order to achieve the objectives of the Organization in question legally, does not violate the law and in accordance with morals or ethics. Double role conflict can have an impact on work stress, this shows the higher conflict dual role female employees, then the greater the also stress their work. The impact of the conflict on performance of dual role female employees indicating higher role conflict the women, then the lower the performance of them. Work stress is also impacting on the performance of female employees have, i.e. show the higher the stress of work, then the lower the pulakinerja female employees.
PERSEPSI SISWA TERHADAP MATA PELAJARAN SEJARAH Septiani, Angelina; Ayu, Dini Melinda; Renaldy, Efessus; Fitriani, Hani; Fauzan, Muhammad Shafwan; Wibowo, Tb. Umar Syarif Hadi; Fitrini, Liska
Cendikia: Jurnal Pendidikan dan Pengajaran Vol. 2 No. 1 (2024): Cendikia: Jurnal Pendidikan dan Pengajaran
Publisher : Cendikia: Jurnal Pendidikan dan Pengajaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.572349/cendikia.v2i1.748

Abstract

Penelitian ini bertujuan guna mengetahui bagaimana persepsi siswa kelas XII terhadap mata pelajaran Sejarah di SMA Negeri 1 Cikeusal. Metode penelitian yang dipakai oleh penulis dalam penelitian ini yaitu dengan menggunakan metode kualitatif. Penelitian ini dilaksanakan di SMAN 1 Cikeusal. Penelitian ini diperoleh dari hasil kuesioner yang dibagikan. Jenis Kuesioner yang digunakan adalah kuesioner tak berstruktur yang dimana kuisioner ini bersifat terbuka dan jawaban responden terhadap setiap pertanyaan kuesioner ini dapat diberikan secara bebas menurut pendapat sendiri. Hasil analisis yang didapat dari di SMA Negeri 1 Cikeusal yaitu dapat diketahui bahwa persepsi siswa yang baik tentang penggunaan media pembelajaran akan lebih mampu mendorong minat dan motivasi siswa mengikuti pembelajaran sejarah, dan jika persepsi siswa sendiri tidak baik dalam penggunaan media pembelajaran justru akan lebih menghambat terjadinya minat siswa untuk mengikuti pembelajaran dengan baik. Metode yang kurang tepat juga mampu membuat siswa memiliki rasa kurang begitu minat terhadap sejarah karena bagi mereka metode yang diterapkan dalam penyampaian materi begitu berpengaruh karena itu juga merupakan strategi dari menarik siswa untuk mau menerima pembelajaran sejarah tanpa memikirkan bosan terhadap mata pelajaran tersebut