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All Journal Jurnal Ilmu Dasar BERKALA SAINSTEK Indonesian Journal of Pure and Applied Chemistry
Wibowo, Muhamad Agus
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Mathematics Physics

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The Bioinsecticide of The Essential Oil of Citronella (Cymbopogon citratus) Against Black Ants (Crematogaster sp) Wibowo, Muhamad Agus; Wahyuni, Sri; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.43923

Abstract

The essential oil of Cymbopogon citratus has the potential as a natural bioinsecticide in safe pest control. This research aimed to determine the chemical content and activity of the essential oil of C. citratus from Punggur village, Kubu Raya district, in killing black ants. This research was conducted in two stages: (i) Distillation of citronella oil and its characterization by IR and GC-MS, and (ii) bioinsecticide test of the oil on black ants (Crematogaster sp). The distillation results gave a yield of 0.43%, which contained 47 compounds with 7 main compounds namely beta.-Myrcene (9.98%), iso-geranial (7.25%), linalool (2.04%), geranyl acetate (2 .39%), geranic acid(1.07%), citral (Z)-(14.97%), and citral (E)-(4.71%). Characterization with IR showed that there were absorption wavelengths of 3500 cm-1 (C-OH), 3086 cm-1 (C-H alkenes), 2920 cm-1 (C H alkanes), 2856 cm-1 and 2748 cm-1 (C-H aldehyde), 1716 cm-1 and 1674 cm-1 (C=O). The anti-ant activity test using various concentrations gave an LCM100 value of 1% and an LC50 of 0.016%. From the results of the study, it can be concluded that the essential oil of citronella leaves is a bioinsecticide against Crematogaster sp.
Characterization of The essential oil of Eucalyptus Leaves (Melaleuca leucadendra) from Pontianak City and Its Activity Against Streptococcus mutans Wibowo, Muhamad Agus; Rendi, R.; Warsida, W.; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.33592

Abstract

Eucalyptus plants can grow in the city of Pontianak, which is an area with peat soil, although in fact, this plant can grow well in swamps, sandy areas, and mangroves. The difference in the location where eucalyptus grows, causes a difference in the composition of the compounds contained in it, especially 1.8 cineol which is a potential compound to prevent dental caries. This study aims to isolate and characterize the essential oil of eucalyptus leaves from the city of Pontianak and determine its activity in inhibiting the S. mutans bacteria. This research was carried out in two stages: isolating, characterizing, and determining the chemical content of eucalyptus leaf essential oil from Pontianak using Gas Chromatography-Mass Spectroscopy (GC-MS). The second stage was to test the antibacterial activity in inhibiting the growth of S. mutans bacteria using the well diffusion method. The results of the isolation of essential oils obtained a yield of 0.58% (w/w) with a specific gravity characteristic of 0.9288 g/mL, a clear greenish-yellow color, and a characteristic smell of eucalyptus oil, and is soluble in 70% ethanol. GC-MS analysis showed that there were 29 compounds, with the main compound being 1.8 cineol as much as 55.71%. The results of the antibacterial activity test against S. mutans showed that the samples at all test doses were antibacteria with inhibition values of 9.03 mm (1.393 mg/well), 6.76 mm (0.697 mg/well), 6.53 mm (0.348 mg/well), and 5.39 mm (0.174 mg/well). The follow-up test showed that at all doses of the essential oil, it was bacteriostatic against S. mutans bacteria. From the study results, it can be concluded that Pontianak eucalyptus oil meets the standards of SNI 06-3954-2006 with 1.8 cineol as the main compound and has antibacteria properties against S. mutans.
Anti-Termite Activities of The Bioactive Compounds of Gaharu Culture (Aetoxylon sympetalum) From Maceration Results Using Acetone Solvent Yuniar, Risa; Jayuska, Afghani; Alimuddin, Andi Hairil; Wibowo, Muhamad Agus; Ardiningsih, Puji
BERKALA SAINSTEK Vol 11 No 2 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i2.38015

Abstract

Agarwood is a plant known for its aromatic resin, which is one of the most widely distributed species in Indonesia. This research was carried out in several stages, namely maceration, partitioning, Thin Layer Chromatography (TLC), and termites activity. The test was carried out for 3 days with variations in the concentration of 0% (negative control), 0.1%, 0.2%. 0.3%, 0.4%, 0.5% (w/v), and 0.25% fipronil reagent as positive controls. The crude extract yield of sapwood gaharu aloe acetone obtained was 23,4321% and the partition result of 233,789 grams of crude extract of acetone consisted of n-hexane fraction with a yield of 3.812%, chloroform fraction of 42.205%, and acetone fraction of 43.621%. The results of the phytochemical test showed that aloe vera sapwood contained alkaloid compounds, flavonoids, steroids, terpenoids, phenolics and saponins. GC-MS analysis of the n-hexane fraction showed the number of peaks as many as 55 peaks which may contain 55 compounds in the fraction. The compound with the highest % area was 4-Chloro-6-methoxy-2-methyl quinoline-8-amine (10.33%) followed by stigmasterol compound (5.94%). The results of the termite activity test showed that the most active fraction as an anti-termite was acetone fraction (LC50 0.082%) followed by chloroform fraction (LC50 0.134%), crude extract (LC50 0.144%) and n-hexane fraction (LC50 0.176%)
PENGARUH LAMA PEMANASAN TERHADAP SIFAT FISIKOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK VCO DARI CAMPURAN KUNYIT, JAHE GAJAH, BAWANG PUTIH DAN DAUN SIRIH (THE INFLUENCE OF HEATING DURATION ON THE PHYSICOCHEMICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF VCO EXTRACT FROM A MIXTURE OF TURMERIC, GINGER, GARLIC, AND BETEL LEAF) Wibowo, Muhamad Agus; Pitri, Sela Rahma; Ardiningsih, Puji; Jayuska, Afghani
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77401

Abstract

The addition of spices to herbal oil increases bioactivity and physicochemical properties. Turmeric, ginger, garlic, and betel leaf are herbal plants that have the potential to enhance the antioxidant activity and physicochemical properties of herbal oil. This study aims to evaluate the effect of heating temperature on the phytochemical content, physicochemical properties, and antioxidant activity of VCO-based herbal oil with the addition of these spices. The research methods include phytochemical analysis, physicochemical analysis, and antioxidant activity testing using the DPPH method. The herbal oil was prepared at 100°C with a ratio of herbal plants to VCO of 4:30, and heating durations of 15, 30, 60, and 120 minutes were varied. The results show the presence of flavonoids, terpenoids, saponins, phenolics, and alkaloids in the herbal oil, while VCO contains flavonoids, terpenoids, saponins, and alkaloids, with the addition of herbal plants providing additional phenolic compounds. Physicochemical testing of the herbal oil formulations with heating durations of 15; 30; 60; and 120 minutes respectively shows water content of 0.0923; 0.0189; 0.0165; and 0.0123%, density of 0.98522; 1.02854; 1.55110; and 1.1227 g/mL, acidity level of 4.12; 4.80; 4.28; and 4.38, free fatty acid content of 0.0018; 0.0021; 0.00162, and 0.0029%, and peroxide value of 0.0021; 0.0035; 0.0035; and 0.0042 meq/kg. The IC50 value obtained from the antioxidant activity testing of the herbal oil formulation with a heating duration of 60 minutes is 577.3 ppm, while for VCO, the IC50 obtained is 1029.6 ppm. This indicates that the addition of spices and herbal plants can enhance the bioactivity of VCO-based herbal oil formulations. The addition of spices and herbal plants increases phytochemical content and antioxidant activity, but longer heating durations may decrease the physicochemical properties of herbal oil formulations
Co-Authors Andi Hairil Alimuddin Jayuska, Afghani Pitri, Sela Rahma Puji Ardiningsih Rendi, R. Sri Wahyuni Warsida, W. Yuniar, Risa