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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Innofarm : Jurnal Inovasi Pertanian Agrointek Indonesian Journal of Cancer Chemoprevention KOVALEN: Jurnal Riset Kimia Al-Kimia Indonesian Journal of Pure and Applied Chemistry CHEESA: Chemical Engineering Research Articles Jurnal Teknologi Kimia Unimal al Kimiya : Jurnal Ilmu Kimia dan Terapan
Puji Ardiningsih
Department of Chemistry, Mathematics and Natural Sciences Faculty, Tanjungpura University, Jl. A. Yani, Pontianak, Kalimantan Barat, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics

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Antimicrobial, Antioxidant, Hemolytic Activities and Toxicity of Ethyl Acetate Extract From an Unidentified Coral-Associated Fungus, Aspergillus brevipes RK06 Nofiani, Risa; Kurniadi, Rio; Ardiningsih, Puji
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Marine fungi are one of the potential  and prolific sources to produce unique and novel structure of bioactive compounds. The aim of this research was to explore the biological activities potency from ethyl acetate extract of Aspergillus brevipes  RK06. A. brevipes  RK06 was successfully isolated from an unidentified coral from Randayan Island, Kalimantan Barat. The  extract inhibited the oxidation of linoleic acid (Ferric thiocyanate assay) with a lipid peroxidation inhibition value of 28.44%. The IC50 value of the extract for brine shrimp lethality test was 34.19ug/mL. The hemolytic percentage of the extract for hemolysis on cow erythrocytes was 5.21%. The extract showed a growth inhibition against  Klebsiella pneumonia,  Pseudomonas aeruginosa, and Staphylococcus aureus. Based on the assays, the extract showed a potential citotoxity and both low antioxidant and hemolytic activities.   Key words: antioxidant, hemolytic activity, antimicrobial, toxicity,  Aspergillus brevipes RK06
A Study of Mercury Pollution in Water, Sediment, and Lais (Kryptopterus) Fish in the Melawi Watershed Triswanto, Bambang; Widodo, Muhammad Agus; Ardiningsih, Puji
Journal of Tropical Life Science Vol 10, No 3 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.03.04

Abstract

Mercury (Hg) is a heavy metal that is very dangerous and toxic. Melawi River, a river with a length of 471 m, passes through four districts namely Menukung, Ella Hulu, Ella Hilir, and Nanga Pinoh which are the main water transportation routes in Melawi regency. This research was aimed to determine the concentration of mercury in water, sediments and Lais (Kryptopterus) fish of mercury bioconcentration factors, and the safety level of consumption the contaminated Lais (Kryptopterus) fish. The mercury measurement using Atomic Absorption Spectroscopy (AAS) showed that the average concentration of mercury found in the water was 1.80×10-4 ppm, sediments 1.33×10-2 ppm, and Lais fish 0.291 ppm. Meanwhile, the average bioconcentration factor (BCF) of mercury in Lais fish was 1.677 ppm. The maximum daily consumption of Lais fish that were safe for the community consumption at the Melawi watershed was 0,026 kg /day for adults and 0.006 kg/day for the children.
Karakterisasi Biochar dari Ampas Tebu dan Kemampuan Penyerapan Nitrogen Sebagai Amelioran Pada Tanah Gambut Secara In Vitro Warsidah, Warsidah; Suparnawati, Suparnawati; Aritonang, Anthoni Batahan; Ardiningsih, Puji; Ashari, Asri Mulya; Sofiana, Mega Sari Juane
Innofarm:Jurnal Inovasi Pertanian Vol 23, No 1 (2021): Innofarm : Jurnal Inovasi Pertanian
Publisher : FAKULTAS PERTANIAN UNIVERSITAS SLAMET RIYADI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/innofarm.v23i1.5604

Abstract

Research has been carried out on the characterization of biochar pores produced from bagasse through a pyrolysis process at a temperature of 110oC for 4 hours, using the Scaning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) method. The biochar of sugarcane pulp produced is then tested for its ability to absorb nitrogen content (% N-total), with the source of N is urea fertilizer through the Kjedhal method based on SNI (2801: 2010), and the source of nitrogen from a solution of ammonium sulfate (NH4)2SO4 by the titrimetric method. The SEM measurement results showed that the biochar pores were uniform and micro-sized, while the peat pores were macro in size and irregular. EDX results show that biochar is composed of 100% carbon, while peat soil consists of carbon compounds (C), Oxygen (O) Silica (Si) and Aluminum (Al). Both of biochar and peat have moisture content of 0.38% and 11.79%, ash content of 2.42% AND 24.44%, volatile content of 0.53% and 5.23% of bound or fixed carbon (cf) of 97.02% and 58.53%. The results of the biochar capacity test applied to peat soils can increase the pH of the peat from 5 to 6.5 with the ability to absorb N-total from urea (NH2)2CO and N-total fertilizer sources from ammonium sulfate (NH4)2SO4 solution of 38% - 66%, and 56.5% -84.5%.
Secondary Metabolites and Antioxidant Activity of Methanol Extract and Fractions from the Root of Gitaan (Willughbeia coreacea Wall) Rudiyansyah; Edwindar, Felgi; Ardiningsih, Puji
Al-Kimia Vol 11 No 2 (2023): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

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Abstract

Gitaan (Willughbeia coreaceae) is one of Indonesian endemic plants and belongs to Apocynaceae family that is potential for antioxidant source. This family contains triterpenoids, phenolics, alkaloids, and cardenolides, unfortunately information about secondary metabolites together with its biological activities from W. coreaceae is scarce. This study was conducted to determine secondary metabolites and antioxidant activity from the methanol extract and fractions of gitaan root. The root powder was macerated, its secondary metabolites were analyzed by phytochemical test and the antioxidant activity was evaluated by the qualitative and quantitative DPPH (1,1-diphenyl-2-picrylhydrazil) methods with the ascorbic acid was used as a positive control. Results showed the methanol extract and methanol fraction have alkaloids, polyphenols, and terpenoids, and ethyl acetate and n-hexane fractions contained alkaloids and polyphenols. By DPPH qualitative, all samples except for n-hexane fraction showed antioxidant properties which were indicated by yellow stains on the thin layer chromatography (TLC) plate after sprayed with 50 ppm of DPPH. By quantitative, methanol extract and fractions of methanol, ethyl acetate, n-hexane, and the ascorbic acid showed antioxidant activity with IC50 values of 44.1181 ppm, 45.4060 ppm, 57.2484 ppm, 983.9576 ppm, and 0.0445 ppm, respectively. Subsequently, methanol extract has better antioxidant activity then other fractions.
The Bioinsecticide of The Essential Oil of Citronella (Cymbopogon citratus) Against Black Ants (Crematogaster sp) Wibowo, Muhamad Agus; Wahyuni, Sri; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.43923

Abstract

The essential oil of Cymbopogon citratus has the potential as a natural bioinsecticide in safe pest control. This research aimed to determine the chemical content and activity of the essential oil of C. citratus from Punggur village, Kubu Raya district, in killing black ants. This research was conducted in two stages: (i) Distillation of citronella oil and its characterization by IR and GC-MS, and (ii) bioinsecticide test of the oil on black ants (Crematogaster sp). The distillation results gave a yield of 0.43%, which contained 47 compounds with 7 main compounds namely beta.-Myrcene (9.98%), iso-geranial (7.25%), linalool (2.04%), geranyl acetate (2 .39%), geranic acid(1.07%), citral (Z)-(14.97%), and citral (E)-(4.71%). Characterization with IR showed that there were absorption wavelengths of 3500 cm-1 (C-OH), 3086 cm-1 (C-H alkenes), 2920 cm-1 (C H alkanes), 2856 cm-1 and 2748 cm-1 (C-H aldehyde), 1716 cm-1 and 1674 cm-1 (C=O). The anti-ant activity test using various concentrations gave an LCM100 value of 1% and an LC50 of 0.016%. From the results of the study, it can be concluded that the essential oil of citronella leaves is a bioinsecticide against Crematogaster sp.
Characterization of The essential oil of Eucalyptus Leaves (Melaleuca leucadendra) from Pontianak City and Its Activity Against Streptococcus mutans Wibowo, Muhamad Agus; Rendi, R.; Warsida, W.; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.33592

Abstract

Eucalyptus plants can grow in the city of Pontianak, which is an area with peat soil, although in fact, this plant can grow well in swamps, sandy areas, and mangroves. The difference in the location where eucalyptus grows, causes a difference in the composition of the compounds contained in it, especially 1.8 cineol which is a potential compound to prevent dental caries. This study aims to isolate and characterize the essential oil of eucalyptus leaves from the city of Pontianak and determine its activity in inhibiting the S. mutans bacteria. This research was carried out in two stages: isolating, characterizing, and determining the chemical content of eucalyptus leaf essential oil from Pontianak using Gas Chromatography-Mass Spectroscopy (GC-MS). The second stage was to test the antibacterial activity in inhibiting the growth of S. mutans bacteria using the well diffusion method. The results of the isolation of essential oils obtained a yield of 0.58% (w/w) with a specific gravity characteristic of 0.9288 g/mL, a clear greenish-yellow color, and a characteristic smell of eucalyptus oil, and is soluble in 70% ethanol. GC-MS analysis showed that there were 29 compounds, with the main compound being 1.8 cineol as much as 55.71%. The results of the antibacterial activity test against S. mutans showed that the samples at all test doses were antibacteria with inhibition values of 9.03 mm (1.393 mg/well), 6.76 mm (0.697 mg/well), 6.53 mm (0.348 mg/well), and 5.39 mm (0.174 mg/well). The follow-up test showed that at all doses of the essential oil, it was bacteriostatic against S. mutans bacteria. From the study results, it can be concluded that Pontianak eucalyptus oil meets the standards of SNI 06-3954-2006 with 1.8 cineol as the main compound and has antibacteria properties against S. mutans.
Anti-Termite Activities of The Bioactive Compounds of Gaharu Culture (Aetoxylon sympetalum) From Maceration Results Using Acetone Solvent Yuniar, Risa; Jayuska, Afghani; Alimuddin, Andi Hairil; Wibowo, Muhamad Agus; Ardiningsih, Puji
BERKALA SAINSTEK Vol 11 No 2 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i2.38015

Abstract

Agarwood is a plant known for its aromatic resin, which is one of the most widely distributed species in Indonesia. This research was carried out in several stages, namely maceration, partitioning, Thin Layer Chromatography (TLC), and termites activity. The test was carried out for 3 days with variations in the concentration of 0% (negative control), 0.1%, 0.2%. 0.3%, 0.4%, 0.5% (w/v), and 0.25% fipronil reagent as positive controls. The crude extract yield of sapwood gaharu aloe acetone obtained was 23,4321% and the partition result of 233,789 grams of crude extract of acetone consisted of n-hexane fraction with a yield of 3.812%, chloroform fraction of 42.205%, and acetone fraction of 43.621%. The results of the phytochemical test showed that aloe vera sapwood contained alkaloid compounds, flavonoids, steroids, terpenoids, phenolics and saponins. GC-MS analysis of the n-hexane fraction showed the number of peaks as many as 55 peaks which may contain 55 compounds in the fraction. The compound with the highest % area was 4-Chloro-6-methoxy-2-methyl quinoline-8-amine (10.33%) followed by stigmasterol compound (5.94%). The results of the termite activity test showed that the most active fraction as an anti-termite was acetone fraction (LC50 0.082%) followed by chloroform fraction (LC50 0.134%), crude extract (LC50 0.144%) and n-hexane fraction (LC50 0.176%)
AKTIVITAS ANTIBAKTERI KURKUMIN DAN KOMPLEKS TEMBAGA (II) KURKUMIN TERHADAP S.AUREUS DAN E.COLI Firdaus, Giska Ristianty; Silalahi, Imelda Hotmarisi; Ardiningsih, Puji
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77806

Abstract

Copper (II) complex of curcumin was prepared from the reaction between curcumin and a metal precursor (CuCl2.2H2O) in ethanol with a mole ratio curcumin to metal 2:1. The FTIR spectrum of Cu2+-curcumin shows a change in the absorption band and a shift in the wave number, especially in the absorption band of the phenolic –OH group 3510.45 cm-1 which is a stretching vibration that becomes wider and shifts to a larger wave number, namely 3514.30 cm-1. Compared with free curcumin spectrum the absorption band of the C=O functional group shows a change where in free curcumin is at 1627.92 cm-1 while in the Cu2+-curcumin complex the is at 1735.93 cm-1 this indicate the coordination of metal ions with curcumin. The IR spectrum shows a typical absorption band for the metal oxygen group, Cu-O 480.28 cm-1 in the Cu2+-curcumin complex. The UV-Vis spectrum of the Cu2+-curcumin complex show a shift towards a larger wavelength (red shift/batochromic) of (~2 nm). Antibacterial activity of Cu2+-curcumin complex compound is active in inhibiting S.aureus and E.coli. After complexation, the activity against the tested microorganisms generally increased and the complex showed higher activity than free curcumin.
PENGARUH LAMA PEMANASAN TERHADAP SIFAT FISIKOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK VCO DARI CAMPURAN KUNYIT, JAHE GAJAH, BAWANG PUTIH DAN DAUN SIRIH (THE INFLUENCE OF HEATING DURATION ON THE PHYSICOCHEMICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF VCO EXTRACT FROM A MIXTURE OF TURMERIC, GINGER, GARLIC, AND BETEL LEAF) Wibowo, Muhamad Agus; Pitri, Sela Rahma; Ardiningsih, Puji; Jayuska, Afghani
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77401

Abstract

The addition of spices to herbal oil increases bioactivity and physicochemical properties. Turmeric, ginger, garlic, and betel leaf are herbal plants that have the potential to enhance the antioxidant activity and physicochemical properties of herbal oil. This study aims to evaluate the effect of heating temperature on the phytochemical content, physicochemical properties, and antioxidant activity of VCO-based herbal oil with the addition of these spices. The research methods include phytochemical analysis, physicochemical analysis, and antioxidant activity testing using the DPPH method. The herbal oil was prepared at 100°C with a ratio of herbal plants to VCO of 4:30, and heating durations of 15, 30, 60, and 120 minutes were varied. The results show the presence of flavonoids, terpenoids, saponins, phenolics, and alkaloids in the herbal oil, while VCO contains flavonoids, terpenoids, saponins, and alkaloids, with the addition of herbal plants providing additional phenolic compounds. Physicochemical testing of the herbal oil formulations with heating durations of 15; 30; 60; and 120 minutes respectively shows water content of 0.0923; 0.0189; 0.0165; and 0.0123%, density of 0.98522; 1.02854; 1.55110; and 1.1227 g/mL, acidity level of 4.12; 4.80; 4.28; and 4.38, free fatty acid content of 0.0018; 0.0021; 0.00162, and 0.0029%, and peroxide value of 0.0021; 0.0035; 0.0035; and 0.0042 meq/kg. The IC50 value obtained from the antioxidant activity testing of the herbal oil formulation with a heating duration of 60 minutes is 577.3 ppm, while for VCO, the IC50 obtained is 1029.6 ppm. This indicates that the addition of spices and herbal plants can enhance the bioactivity of VCO-based herbal oil formulations. The addition of spices and herbal plants increases phytochemical content and antioxidant activity, but longer heating durations may decrease the physicochemical properties of herbal oil formulations
Induksi Aquilaria malaccensis Menggunakan Kombinasi Fusarium oxysporum dan Asam Salisilat: Aquilaria malaccensis Induction Using a Combination of Fusarium oxysporum and Salicylic Acid Rani, Diah Puspa; Jayuska, Afghani; Khotimah, Siti; Ardiningsih, Puji; Agus Wibowo, Muhammad
KOVALEN: Jurnal Riset Kimia Vol. 10 No. 3 (2024): December Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2024.v10.i3.17041

Abstract

Agarwood is a resin from agarwood-producing plants that are given certain stimulants, thus stimulating the tree's defense system. The defense system produced is in the form of a sweet-smelling phytoalexin compound, better known as agarwood. Stimulants that can stimulate the formation of Gaharu are biological inoculants and chemical inducers. In this research, the biological inoculant used the Fusarium oxysporum fungus, and the chemical inducer used salicylic acid. This research aims to determine the effectiveness of using a combination of Fusarium oxysporum and salicylic acid in producing agarwood resin. The combination treatment of Fusarium oxysporum and salicylic acid was carried out using the injection method on Aquilaria malaccensis tree branches, and induction was carried out for 3 months. This research shows that the TLC test results in all treatments contain aloes, which is proven by the discovery of phenolic and terpenoid compounds. The combination treatment produces a color change intensity that is not darker and has a broader color change zone compared to the single treatment. Therefore, it can be concluded that the combination of Fusarium oxysporum and salicylic acid is less effective in producing Gaharu than the single treatment.
Co-Authors Adhitiyawarman, Adhitiyawarman Agus Wibowo, Muhammad Ajuk Sapar Alatin, Isam Andi Hairil Alimuddin Ari Widiyantoro Aritonang, Anthoni Batahan Ashari, Asri Mulya Edwindar, Felgi Firdaus, Giska Ristianty Hasanah, Kutsiatul Hendra Perdana Imelda Hotmarisi Silalahi Jayuska, Afghani Juniarti, Leni Mega Sari Juane Sofiana Millenia, Millenia Nurul Huda Pitri, Sela Rahma Rani, Diah Puspa Rendi, R. Rio Kurniadi Risa Nofiani Roeswandi, Irine Fajrin Rudiyansyah Rudiyansyah Rudiyansyah Sadenta, Deby Pascalia Sari, Heni Puspita Siti Khotimah Sri Wahyuni Sulistyo, Luthfi Imam Suparnawati, Suparnawati Triswanto, Bambang Warsida, W. Warsidah, Warsidah Wibowo, Muhamad Agus Widodo, Muhammad Agus Yuniar, Risa