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PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) PADA INDUK LAKTASI UNTUK MENINGKATKAN BOBOT BADAN ANAK MENCIT Panjaitan, Ruqiah Ganda Putri; Jayuska, Afghani; Harahap, Zulfan; Zakiah, Zulfa
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

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The Effect of Administration of Pasak Bumi (Eurycoma longifolia Jack.) Roots on Lactating Mice to Increase Body Weight Pups. The roots of Eurycoma longifolia Jack have been used as a traditional medicine for aprodisiac. The effects of the root extract of E. longifolia Jack were studied on lactating mice. Each mice was administered metanol extract and its derived fractions at dose 500 mg/kg rat body weight for 21 consecutive days in lactation period. Control positive group received Moloco+B12 at dose 0,13 g/kg rat body weight, negative control (placebo) group received 2 ml/kg rat body weight of aquadest daily and normal control group. No differences between normal control, negative control, positive control, and metanol extract and its derived fractions. The administration methanol-water fraction to lactating mice increasing body weight mice pups similar to Moloco+B12.

PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) PADA INDUK LAKTASI UNTUK MENINGKATKAN BOBOT BADAN ANAK MENCIT Panjaitan, Ruqiah Ganda Putri; Jayuska, Afghani; Harahap, Zulfan; Zakiah, Zulfa
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

The Effect of Administration of Pasak Bumi (Eurycoma longifolia Jack.) Roots on Lactating Mice to Increase Body Weight Pups. The roots of Eurycoma longifolia Jack have been used as a traditional medicine for aprodisiac. The effects of the root extract of E. longifolia Jack were studied on lactating mice. Each mice was administered metanol extract and its derived fractions at dose 500 mg/kg rat body weight for 21 consecutive days in lactation period. Control positive group received Moloco+B12 at dose 0,13 g/kg rat body weight, negative control (placebo) group received 2 ml/kg rat body weight of aquadest daily and normal control group. No differences between normal control, negative control, positive control, and metanol extract and its derived fractions. The administration methanol-water fraction to lactating mice increasing body weight mice pups similar to Moloco+B12.

The Bioinsecticide of The Essential Oil of Citronella (Cymbopogon citratus) Against Black Ants (Crematogaster sp) Wibowo, Muhamad Agus; Wahyuni, Sri; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.43923

The essential oil of Cymbopogon citratus has the potential as a natural bioinsecticide in safe pest control. This research aimed to determine the chemical content and activity of the essential oil of C. citratus from Punggur village, Kubu Raya district, in killing black ants. This research was conducted in two stages: (i) Distillation of citronella oil and its characterization by IR and GC-MS, and (ii) bioinsecticide test of the oil on black ants (Crematogaster sp). The distillation results gave a yield of 0.43%, which contained 47 compounds with 7 main compounds namely beta.-Myrcene (9.98%), iso-geranial (7.25%), linalool (2.04%), geranyl acetate (2 .39%), geranic acid(1.07%), citral (Z)-(14.97%), and citral (E)-(4.71%). Characterization with IR showed that there were absorption wavelengths of 3500 cm-1 (C-OH), 3086 cm-1 (C-H alkenes), 2920 cm-1 (C H alkanes), 2856 cm-1 and 2748 cm-1 (C-H aldehyde), 1716 cm-1 and 1674 cm-1 (C=O). The anti-ant activity test using various concentrations gave an LCM100 value of 1% and an LC50 of 0.016%. From the results of the study, it can be concluded that the essential oil of citronella leaves is a bioinsecticide against Crematogaster sp.

Characterization of The essential oil of Eucalyptus Leaves (Melaleuca leucadendra) from Pontianak City and Its Activity Against Streptococcus mutans Wibowo, Muhamad Agus; Rendi, R.; Warsida, W.; Ardiningsih, Puji; Jayuska, Afghani
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.33592

Eucalyptus plants can grow in the city of Pontianak, which is an area with peat soil, although in fact, this plant can grow well in swamps, sandy areas, and mangroves. The difference in the location where eucalyptus grows, causes a difference in the composition of the compounds contained in it, especially 1.8 cineol which is a potential compound to prevent dental caries. This study aims to isolate and characterize the essential oil of eucalyptus leaves from the city of Pontianak and determine its activity in inhibiting the S. mutans bacteria. This research was carried out in two stages: isolating, characterizing, and determining the chemical content of eucalyptus leaf essential oil from Pontianak using Gas Chromatography-Mass Spectroscopy (GC-MS). The second stage was to test the antibacterial activity in inhibiting the growth of S. mutans bacteria using the well diffusion method. The results of the isolation of essential oils obtained a yield of 0.58% (w/w) with a specific gravity characteristic of 0.9288 g/mL, a clear greenish-yellow color, and a characteristic smell of eucalyptus oil, and is soluble in 70% ethanol. GC-MS analysis showed that there were 29 compounds, with the main compound being 1.8 cineol as much as 55.71%. The results of the antibacterial activity test against S. mutans showed that the samples at all test doses were antibacteria with inhibition values of 9.03 mm (1.393 mg/well), 6.76 mm (0.697 mg/well), 6.53 mm (0.348 mg/well), and 5.39 mm (0.174 mg/well). The follow-up test showed that at all doses of the essential oil, it was bacteriostatic against S. mutans bacteria. From the study results, it can be concluded that Pontianak eucalyptus oil meets the standards of SNI 06-3954-2006 with 1.8 cineol as the main compound and has antibacteria properties against S. mutans.

Anti-Termite Activities of The Bioactive Compounds of Gaharu Culture (Aetoxylon sympetalum) From Maceration Results Using Acetone Solvent Yuniar, Risa; Jayuska, Afghani; Alimuddin, Andi Hairil; Wibowo, Muhamad Agus; Ardiningsih, Puji
BERKALA SAINSTEK Vol 11 No 2 (2023)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v11i2.38015

Agarwood is a plant known for its aromatic resin, which is one of the most widely distributed species in Indonesia. This research was carried out in several stages, namely maceration, partitioning, Thin Layer Chromatography (TLC), and termites activity. The test was carried out for 3 days with variations in the concentration of 0% (negative control), 0.1%, 0.2%. 0.3%, 0.4%, 0.5% (w/v), and 0.25% fipronil reagent as positive controls. The crude extract yield of sapwood gaharu aloe acetone obtained was 23,4321% and the partition result of 233,789 grams of crude extract of acetone consisted of n-hexane fraction with a yield of 3.812%, chloroform fraction of 42.205%, and acetone fraction of 43.621%. The results of the phytochemical test showed that aloe vera sapwood contained alkaloid compounds, flavonoids, steroids, terpenoids, phenolics and saponins. GC-MS analysis of the n-hexane fraction showed the number of peaks as many as 55 peaks which may contain 55 compounds in the fraction. The compound with the highest % area was 4-Chloro-6-methoxy-2-methyl quinoline-8-amine (10.33%) followed by stigmasterol compound (5.94%). The results of the termite activity test showed that the most active fraction as an anti-termite was acetone fraction (LC50 0.082%) followed by chloroform fraction (LC50 0.134%), crude extract (LC50 0.144%) and n-hexane fraction (LC50 0.176%)

Essential Oils Activity of Legundi Leaf (Vitex trifolia L.) as A Repellent for Rice Weevil (Sitophilus oryzae) Jayuska, Afghani; Warsidah, Warsidah; Asikin, Nurul; Widiyantoro, Ari; Aritonang, Anthoni B.
BERKALA SAINSTEK Vol 10 No 1 (2022)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v10i1.31011

Legundi (Vitex trifolia L.) is a plant that contains essential oils. Legundi leaf essential oil has the potential as a more environmentally friendly rice lice repellent. The process of extracting essential oils from Legundi leaves uses the steam-water distillation method. The yield obtained from distillation is 0.10% with a bright yellow color, has a distinctive smell of Legundi oil with a specific gravity of 0.9065 gram/cm3. GC-MS search results showed that Legundi leaf essential oil contains 5 main components, namely 2-β-Pinene (16.18 %), trans-caryophyllene (13.75 %), β-Ocimene (11.16 %), Cyclohexanol (10.03%), and Eucalyptol (5.45%). Testing of repellent activity was carried out on rice lice with variations in volatile oil concentrations of 1%, 5%, 10%, and 20%. The results of the repellent test showed that the highest percentage occurred at a concentration of 20% (P4), which was 56.7%, with an application time of 120 hours (L5). Based on this, it can be said that the greater the concentration of the essential oil used, the higher the percentage of rejection of the population, this also indicates that Legundi leaf essential oil has lice repellent activity (Sitophilus oryzae).

PENGARUH LAMA PEMANASAN TERHADAP SIFAT FISIKOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK VCO DARI CAMPURAN KUNYIT, JAHE GAJAH, BAWANG PUTIH DAN DAUN SIRIH (THE INFLUENCE OF HEATING DURATION ON THE PHYSICOCHEMICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF VCO EXTRACT FROM A MIXTURE OF TURMERIC, GINGER, GARLIC, AND BETEL LEAF) Wibowo, Muhamad Agus; Pitri, Sela Rahma; Ardiningsih, Puji; Jayuska, Afghani
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77401

The addition of spices to herbal oil increases bioactivity and physicochemical properties. Turmeric, ginger, garlic, and betel leaf are herbal plants that have the potential to enhance the antioxidant activity and physicochemical properties of herbal oil. This study aims to evaluate the effect of heating temperature on the phytochemical content, physicochemical properties, and antioxidant activity of VCO-based herbal oil with the addition of these spices. The research methods include phytochemical analysis, physicochemical analysis, and antioxidant activity testing using the DPPH method. The herbal oil was prepared at 100°C with a ratio of herbal plants to VCO of 4:30, and heating durations of 15, 30, 60, and 120 minutes were varied. The results show the presence of flavonoids, terpenoids, saponins, phenolics, and alkaloids in the herbal oil, while VCO contains flavonoids, terpenoids, saponins, and alkaloids, with the addition of herbal plants providing additional phenolic compounds. Physicochemical testing of the herbal oil formulations with heating durations of 15; 30; 60; and 120 minutes respectively shows water content of 0.0923; 0.0189; 0.0165; and 0.0123%, density of 0.98522; 1.02854; 1.55110; and 1.1227 g/mL, acidity level of 4.12; 4.80; 4.28; and 4.38, free fatty acid content of 0.0018; 0.0021; 0.00162, and 0.0029%, and peroxide value of 0.0021; 0.0035; 0.0035; and 0.0042 meq/kg. The IC50 value obtained from the antioxidant activity testing of the herbal oil formulation with a heating duration of 60 minutes is 577.3 ppm, while for VCO, the IC50 obtained is 1029.6 ppm. This indicates that the addition of spices and herbal plants can enhance the bioactivity of VCO-based herbal oil formulations. The addition of spices and herbal plants increases phytochemical content and antioxidant activity, but longer heating durations may decrease the physicochemical properties of herbal oil formulations

PROFIL GC-MS DAN BIOAKTIVITAS EKSTRAK MINYAK DAUN CENGKEH (Syzygium aromaticum) TERHADAP ULAT GRAYAK Spodoptera frugiperda (GC-MS PROFILING AND BIOACTIVITY OF EXTRACTS CLOVE LEAF OIL (Syzygium aromaticum) AGAINST FALL ARMYWORMS Spodoptera frugiperda) Sitohang, Mikael; Jayuska, Afghani; Hernowo, Kukuh; Alimuddin, Andi Hairil; Aritonang, Anthoni Batahan
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77091

Clove leaf essential oil can be used as a natural insecticide to control pests. Essential oil obtained by steam distillation produces essential oil with a yield of 1.03%. The essential oil obtained was then analyzed using a GC-MS tool where the aim was to separate the mixture of compounds and identify the compounds contained in clove leaf essential oil. The results of GC-MS analysis of clove leaf essential oil shoved 6 compounds whose similarity was above 84%, where the main compounds were eugenol (74.28%), β-Caryophellene (20.49%), α-Caryophellene (2.32%), Caryophellene oxide (2.26%). The essential oils were then tested for mortality and antifeedant activity against 3rd larva S. frugiperda. Concentrations of 0.1%, 0.3%, 0.5%, 0.7% and 0.9% v/v the essential oil caused 43.44%, 56.66%, 70%, 76.77%, and 86.66% mortality on to tested larva. The probit analysis data the mortality resulted LC50 value of 0.58%. Antifeedant concentrations of 0.37% and 0.58% v/v or equivalent to LC25 and LC50 can reduce 42.41-71.96% of the preferred method and 66.46-86.59% of the non-optioned method.

Induksi Aquilaria malaccensis Menggunakan Kombinasi Fusarium oxysporum dan Asam Salisilat: Aquilaria malaccensis Induction Using a Combination of Fusarium oxysporum and Salicylic Acid Rani, Diah Puspa; Jayuska, Afghani; Khotimah, Siti; Ardiningsih, Puji; Agus Wibowo, Muhammad
KOVALEN: Jurnal Riset Kimia Vol. 10 No. 3 (2024): December Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2024.v10.i3.17041

Agarwood is a resin from agarwood-producing plants that are given certain stimulants, thus stimulating the tree's defense system. The defense system produced is in the form of a sweet-smelling phytoalexin compound, better known as agarwood. Stimulants that can stimulate the formation of Gaharu are biological inoculants and chemical inducers. In this research, the biological inoculant used the Fusarium oxysporum fungus, and the chemical inducer used salicylic acid. This research aims to determine the effectiveness of using a combination of Fusarium oxysporum and salicylic acid in producing agarwood resin. The combination treatment of Fusarium oxysporum and salicylic acid was carried out using the injection method on Aquilaria malaccensis tree branches, and induction was carried out for 3 months. This research shows that the TLC test results in all treatments contain aloes, which is proven by the discovery of phenolic and terpenoid compounds. The combination treatment produces a color change intensity that is not darker and has a broader color change zone compared to the single treatment. Therefore, it can be concluded that the combination of Fusarium oxysporum and salicylic acid is less effective in producing Gaharu than the single treatment.

Insecticidal Activity of Cymbopogon citratus and Melaleuca leucadendra Essential Oils and Their Joint Actions Against Spodoptera frugiperda Syahputra, Edy; Jayuska, Afghani; Satrio, Bagus Sandi; Wibowo, Vicy Arif
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4785

A high population of Spodoptera frugiperda larvae reduces corn crop productivity. One alternative insect pest control method is the use of mixed essential oils. This research aims to evaluate the insecticidal and antifeedant activity of lemongrass (Cymbopogon citratus) and melaleuca (Melaleuca leucadendra) essential oils against S. frugiperda larvae, as well as their combined effect. The essential oils were produced using a steam distillation method. The insecticidal activities were assayed using feeding method. The levels of essential oil concentration tested and controlled were repeated five times. The concentration-mortality relationship was analyzed using probit. Antifeedant activity tested by choice and no-choice at sub-lethal concentrations equivalent to LC10,LC25, and LC50. The test results showed that insecticidal activity of the essential oils of C. citratus higher than M leucadendra. Both essential oils showed LC50 values of 0.9% and 1.1% respectively. Both essential oils also showed antifeedant activity. In the choice test and no-choice test at LC10,LC25, and LC50 showed feeding inhibition of 23.7%-56.7%. The mixture of the two essential oils has a strongly synergistic action with an LC50 of 0.15% and a combination index of 0.15. Further research is needed to evaluate the extract mixture in the field.

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