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Hidayat, Nu'man -
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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DATE EXTRACT SUPPLEMENTATION IN RINGER LACTATE-EGG YOLK EXTENDER ON POST-THAWING QUALITY OF PELUNG CHICKEN SPERMATOZOA Hidayat, Nu'man -; Ismoyowati, Ismoyowati; Tugiyanti, Elly; Suswoyo, Imam; Rosidi, Rosidi; Sulistyawan, Ibnu Hari; Nugroho, Aras Prasetyo
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.24317

Abstract

The research purpose was to evaluate the influence of date extract supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate (LREYS) on post-thawing quality of Pelung chicken spermatozoa. Completely randomized design with 5 treatments and 4 replications was used in this study. Semen was collected from three Pelung chickens once in three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of date extract supplementation that were 0% as control (LREYSDE0), 0.5% (LREYSDE1), 1% (LREYSDE2), 1.5% (LREYSDE3), and 2% (LREYSDE4). The post thawing liquid semen was observed for sperm motility, viability, plasma membrane integrity and acrosome integrity. The result showed that the motility, viability, membrane plasma integrity and acrosomal integrity of spermatozoa in LREYSE extender with 1.5% date extract supplementation (30.441.02%; 49.830.91%; 43.261.02%; and 45.330.72% respectively) was significantly higher (P0.05) than others treatment that were 0% (18.210.43%; 35.620.51%; 30.540.60%; and 31.490.71% respectively), 0.5% (20.450.72%; 40.720.87%; 36.810.55%; and 38.560.63% respectively), 1% (25.680.93%; 46.270.75%; 40.521.02%; and 42.831.09% respectively) and 2% (22.550.92%; 43,710,74%; 38,840,71%; and 41,390,86% respectively) of date extract supplementation. It is concluded that the 1.5% date extract supplementation in extender can maintain the best post-thawing sperm quality.
HONEY SUPPLEMENTATION IN LACTATE RINGER-EGG YOLK EXTENDER ON QUALITY OF PELUNG CHICKEN SPERMATOZOA POST-CHILLING Hidayat, Nu'man -; Ismoyowati, Ismoyowati -; Hidayah, Chomsiatun Nurul; Nugroho, Aras Prasetiyo
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18556

Abstract

The purpose of this research was to determine the influence of honey supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E addition (LREYSE) on the quality of Pelung chicken spermatozoa preserved at 5 C for 72 hours.Semen was collected from three Pelung chickens once per day over a course of three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of honey supplementation that are 0% as control (LREYSEH0), 1% (LREYSEH1), 2% (LREYSEH2), 3% (LREYSEH3), and 4% (LREYSEH4). This liquid semen was observed for sperm motility and viability every 12 hours. Complete random designrepeated measurement with 4 replications was used in this study. The results showed the motility and viability of spermatozoa in LREYSEextender with 2% honey supplementation (61.251.25% and 71.500.74%) was significantly higher (P0.05) than other treatments that are 0% (51.251.25% and 61.881.36%), 1% (52.501.44% and 63.251.38%), 3% (51.251.25% and 61.631.48%), and 4% (50.002.04% and 60.632.29%) of honey supplementation in extender at 36 hours of storage until the end of the observation at 72 hours of incubation. According to the results of this study, it can be concluded that the 2% honey supplementation in extender is the best treatment to maintain sperm motility and viability for 72 hours of storage.
FERTILITY AND FERTILE PERIOD OF DUCK EGGS AFTER ARTIFICIAL INSEMINATION WITH MUSCOVY DUCK SEMEN SUPPLEMENTED WITH VITAMIN C AND E Hidayat, Nu'man -; Ismoyowati, Ismoyowati -; Hidayah, Chomsiatun Nurul; Nugroho, Aras Prasetiyo
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i3.14694

Abstract

The aim of this research was to investigate the influence of duck variants and addition of vitamins into muscovy duck semen on fertility and fertile period of duck eggs after artificial insemination. Semen was collected from five muscovy ducks and divided into 3 treatment groups: without vitamin supplementation (A0), supplementation of 400 g/mL vitamin C (A1), and supplementation of 80 g/ml vitamin E (A2). Each semen was inseminated into female ducks of Magelang (B1) and Mojosari (B2) variants. Complete Random Design was used with 3x2 factorial. The results showed that vitamins and duck variants had no significant interaction (P0.05) with fertility and fertile period. The duck variant had no effect (P0.05) on fertility and fertile period, while the addition of vitamins significantly affected (P0.01) the fertility and fertile period. The addition of 400 g/mL vitamin C increased fertility by 22.280.20% but reduced the fertile period by 7.83.5 days, whereas 80 g/mL of vitamin E increased fertility by 11.572.47% but reduced fertile period by 12.30.9 days. It can be concluded that the addition of 400 g/mL of vitamin C and 80 g/mL of vitamin E in Muscovy duck semen increased fertility but shortened fertile period of duck eggs after artificial insemination.
Co-Authors Aras Prasetiyo Nugroho, Aras Prasetiyo Elly Tugiyanti Hidayah, Chomsiatun Nurul Ibnu Hari Sulistyawan Imam Suswoyo Ismoyowati Ismoyowati, Ismoyowati - Nugroho, Aras Prasetyo Rosidi Rosidi