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All Journal Animal Production : Indonesian Journal of Animal Production ANGON: Journal of Animal Science and Technology Jurnal Kedokteran Hewan
Aras Prasetiyo Nugroho, Aras Prasetiyo
Jurusan Peternakan, Bagian Fisiologi dan Reproduksi Ternak Terapan, Fakultas Peternakan, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Inhibition Activity of Garlic (Allium sativum) Skin Aqueous Extract on Mastitis Causing Microorganisms Syamsi, Afduha Nurus; Pratiwi, Meyta; Nugroho, Aras Prasetiyo
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.846 KB) | DOI: 10.20884/1.jap.2019.21.1.673

Abstract

The study was aimed to identify the effectiveness of the inhibitory activity of garlic skin extract (GSE) with different concentrations on the growth of Staphylococcus aureus, Streptococcus mutants, Escherichia coli, and Candida albicans. The study used the Kirby-Bauer method in a completely randomized (CRD) design with five treatments (positive control, negative control, 5% GSE, 10% GSE, and 15% GSE) and three replicates. The extract was obtained through evaporation of garlic skin macerated with aquadest solvent. The data were subjected to ANOVA, continued with an Honestly Significant Difference (HSD) test. The results showed that GSE concentration (minimum 5-10%) was highly significant to inhibit the growth of mastitis-causing microorganisms.
Oxytocin Hormone Induction on Milk Production in Relation to Dairy Kid Performance Sumaryadi, Mas Yedi; Saleh, Dadang Mulyadi; Nugroho, Aras Prasetiyo; Hidayat, Nu'man; Hidayah, Chomsiatun Nurul
ANIMAL PRODUCTION Vol 21, No 3 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.471 KB) | DOI: 10.20884/1.jap.2019.21.3.747

Abstract

The application of oxytocin induction to milk production in relation to the performance of kids in dairy goats had been carried out in the "PEGUMAS" dairy goat farmer group Gumelar District, Banyumas. This activity aimed to increase milk production and kid performance through the induction of the hormone oxytocin in dairy goats. The approach method was designed in two stages: the first stage carried out the socialization activities and demonstration of techniques for applying IPTEKS, and the second stage applied hormonal technology in the form of oxytocin hormone induction in dairy goats. The material used was dairy goat parity I aged 1.5 - 2 years allocated into 2 groups of 10 individuals each. Group I was dairy goat experiment not induced with the oxytocin hormone as the control. Group II was dairy goat experiment induced with the oxytocin hormone at a dose of 1 ml (10 IU) intramuscularly before milking. Milk production was measured morning and evening as daily milk production in milliliters (ml). Milk was given to the kid of each parent. The kid's performance was measured based on the daily weight gain of the kid during the study. Overall, the application of science and technology could be adopted by farmers, and oxytocin-induced dairy goat milk production (503.2 ml) was significantly higher (P<0.05) than control (305.4 ml) with an increase of 64.77%, with a positive level of correlation (r = 0.45) and contributed 19.83% to the kids daily body weight gain.
Reduction Spermatozoa Motility and Viability on Various Local Chickens During Storage at 5℃ Hidayat, Nu'man; Hidayah, Chomsiatun Nurul; Nugroho, Aras Prasetiyo
ANIMAL PRODUCTION Vol. 22 No. 3 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2020.22.3.64

Abstract

The research compared the changes motility and viability of sperm from various local chickens during storage at 5℃ for 72 hours. Semen was collected every three days using the dorsal-abdominal massage from twelve chickens consisting of Kedu, Sentul and Pelung chicken. semen was diluted in extender contains 90% lactate ringer and 10% egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E (LREYSE). Sperm motility and viability was observed every 12 hours and the measurements were made up to 72 hours of storage. Complete random design repeated measurement with 4 replications was used in this study. One-way analysis of variance was used to analyze the data and followed by Duncan’s Multiple Range Test. The results showed sperm motility declined and sperm viability reduced during storage for all breeds. The significant declined of motility between breeds were only observed at 60 and 72 hours and the reduced viability between breeds was observed at 72 hours of storage. The declined sperm motility for Kedu chicken (31.59±3.26% and 75.36±1.25%) and Pelung chicken (36.11±4.05% and 75.83±5.34%) were significantly lower (p<0.05) than Sentul Chicken (50.39±2.60% and 95.00±5.00%) at 60 and 72 hours of storage respectively, while the decline sperm viability of Kedu chicken (57.59±3.64%) and Pelung chicken (54.39±5.73%) was significantly lower (p<0.05) than Sentul Chicken (90.30±9.70%) after 72 hours storage. It can be concluded that the reduction sperm motility and viability of Kedu and Pelung chicken is lower than Sentul chicken which are stored at 5℃ for 72 hours.
PENGARUH MACAM PENGENCER DAN LAMA PENYIMPANAN PADA SUHU 5oC TERHADAP MOTILITAS, VIABILITAS DAN ABNORMALITAS SPERMATOZOA AYAM KAMPUNG Rilandana, Dipta; Saleh, Dadang Mulyadi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (942.029 KB) | DOI: 10.20884/1.angon.2021.3.2.p184-191

Abstract

Background. The aim of the study was to determine the interaction between types of diluents (Ringer's lactate, skimmed milk, and andromed) and storage time at 5°C on motility, viability, and abnormalities of kampung rooster spermatozoa. Materials and Methods. The research materials used were 7 kampung rooster aged 1-1.5 years as a spermatozoa producer, chicken feed, battery cage and its equipment, spermatozoa storage glass, a set of tools to test the quality of spermatozoa, and 1 ml syringe. Other materials are fresh spermatozoa from 7 free-range kampung rooster, skim milk, lactated Ringer, andromed, distilled water, microscopes, dropper pipettes, object glass, cover glass, cement box, thoma chamber, hematocrit pipette, 3% NaCl solution, eosin negrosin solution, refrigerator, and microtube. The research design used was a completely randomized design (CRD) with a factorial pattern of 2 factors: the P factor, namely the kind of diluent and the W factor, the time of storage at 5°C through 3 treatments. There were 3 replications in each treatment so that there were 27 experimental units with each treatment consisting of 3 different times. Results. The results showed that the interaction between different kinds of diluent treatments and storage time had a significant effect (P<0.05) on the viability of kampung rooster spermatozoa, while the interaction between treatments of types of diluents and storage time on motility and abnormalities of kampung rooster spermatozoa had no significant effect (P>0.05). ANOVA results had a very significant effect on the type of diluent (P<0.01) on motility, while the abnormal effect was not significant (P>0.05). Storage time had a significant (P<0.05) effect on abnormalities and had no significant (P>0.05) effect on motility. The results showed that the best motility of free-range kampung rooster spermatozoa was produced by Ringer's lactate diluent with storage for 10 minutes (92%). The best viability of kampung rooster spermatozoa was produced by andromed diluent with storage for 370 minutes (96.5%). Furthermore, the lowest abnormality of kampung rooster spermatozoa was produced by skim milk diluent with storage for 190 minutes (8.5%).
HUBUNGAN PANJANG, LINGKAR, DAN VOLUME SKROTUM DENGAN VIABILITAS DAN ABNORMALITAS SPERMATOZOA PADA KAMBING PEJANTAN PERANAKAN ETAWAH (PE) Pangestu, Mukti Santoso Aji; Sumaryadi, Mas Yedi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1221.099 KB) | DOI: 10.20884/1.angon.2021.3.3.p252-262

Abstract

Background. The study of the relationship between Length, Circumference, and Volume of the scrotum with Viability and Abnormality of Spermatozoa in Etawa Crossbreed (PE). Goats was carried out from 15 November 2020 until 21 November 2020 at Tani Ternak Sato Guno, Gumelar District, Banyumas Regency, Central Java. Materials and methods. The purpose of this study was to determine the length, circumference, volume of the scrotum with the viability and abnormalities of spermatozoa in Etawa male goats, provide scientific information and expand knowledge in the field of animal husbandry, especially the quality of spermatozoa. This research uses experimental with linear correlation analysis method and linear regression analysis. Results. The results of this study indicate that together the length, circumference, and volume of the scrotum have a very strong relationship (P < 0.01) with spermatozoa viability with a coefficient of determination of 0.825 and a correlation coefficient of r = 0.908. This shows that the viability of spermatozoa is influenced by the length, circumference, and volume of the scrotum by 82.5%, while the remaining 17.5% is influenced by other factors. The results of the next study showed that together the length, circumference, and volume of the scrotum had a very strong relationship (P < 0.01) with spermatozoa abnormalities with a coefficient of determination of 0.918 and a correlation coefficient of r = 0.958. This shows that spermatozoa abnormalities are influenced by the length, circumference, and volume of the scrotum by 91.8%, while the remaining 8.2% are influenced by other factors. It was concluded that the mean length, circumference, and scrotum volume of Etawa Crossbreed Goats were 25.50 ± 1.73 cm, respectively; 15.00 ± 1.28 cm; 85.96 ± 20.39 cm3 with the mean viability and abnormality of spermatozoa were 82.12 ± 5.71% and 4.54 ± 2.35%, respectively. Conclusion. Length, circumference, and volume of the scrotum simultaneously have a very strong relationship with the viability and abnormality of spermatozoa, so that it can be used as a good indicator to estimate the quality of spermatozoa, especially the viability and abnormality of spermatozoa with the respective formulas being Y₁ + = 232,500 - 6,500 X₁ - 3,583 X₂ + 0.212 X₃ and Y₂ += 69,000 + 0.917 X₁ - 3.801 X₂ + 0.103 X₃
PENGARUH SENTRIFUGASI GRADIEN DENSITAS PERCOLL DAN LAMA INKUBASI TERHADAP MORFOMETRI DAN MEMBRAN PLASMA UTUH HASIL SEXING SPERMATOZOA KAMBING PERANAKAN ETAWAH Aprila, Riska; Sumaryadi, Mas Yedi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 6 No 2 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2024.6.2.p187-197

Abstract

Penelitian ini berjudul Pengaruh Sentrifugasi Gradien Densitas Percoll dan Lama Inkubasi Terhadap Morfometri dan Membran Plasma Utuh Hasil Sexing Spermatozoa Kambing Peranakan Etawah. Tujuan penelitian dilakukan untuk mengkaji interaksi gradien percoll dan lama inkubasi terhadap morfometri dan Membran Plasma Utuh (MPU) hasil separasi seks kambing Peranakan Etawah (PE). Materi yang digunakan untuk penelitian yaitu semen kambing Peranakan Etawah (PE) jantan sebanyak 2 ekor dengan umur 3-4 tahun. Rancangan penelitian dilakukan menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 2 x 3 dengan ulangan frekuensi penyadapan sebanyak 3 kali. Hasil analisis variansi perlakuan yang berpengaruh nyata kemudian dilakukan uji lanjut dengan uji Duncan’s Multiple Range Test (DMRT). Lokasi penelitian berada di Laboratorium Kesehatan Ternak dan Experimental Farm Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto, Kabupaten Banyumas, Jawa Tengah. Hasil pengukuran morfometri rata-rata spermatozoa unsexing 30.88 µm2, spermatozoa sexing fraksi atas dan fraksi bawah masing-masing 28.44 µm2 dan 32.63 µm2. Hasil dari penelitian keutuhan membran plasma spermatozoa X diperoleh rataan sebesar X1 = 66.61% dan X2 = 68.57% sedangkan spermatozoa Y diperoleh rataan sebesar Y1 = 64,87% dan Y2 = 66.96%. Hasil perhitungan statistic dengan uji t-student menunjukkan bahwa spermatozoa fraksi bawah (X) memberikan perbedaan nyata (P<0,05) lebih besar dibanding spermatozoa fraksi atas (Y). Berdasaran analisis variansi diperoleh hasil tidak ada interaksi antara faktor gradien dan lama inkubasi (P>0,05) terhadap morfometri dan Membran Plasma Utuh (MPU). Faktor gradien berpengaruh nyata (P<0,05) terhadap morfometri fraksi atas (Y) dan bawah (X), sedangkan faktor gradien dan lama inkubasi masing-masing berpengaruh nyata (P<0,05) terhadap Membran Plasma Utuh (MPU) fraksi atas (Y) dan bawah (X). Kesimpulan yang didapat dari penelitian ini yaitu separasi seks menggunakan medium gradien percoll 45:60% dengan lama inkubasi 30 menit mampu memisah dengan baik morfometrik spermatozoa fraksi atas (Y) maupun fraksi bawah (X), bahkan diperoleh persentase membran plasma utuh (MPU) tertinggi baik untuk fraksi atas dan bawah.
Deteksi Kebuntingan Dini Pada Kambing Peranakan Etawah (Capra aegagrus hircus) Berdasarkan Metode Non-Return Rate dan Reaksi Cubboni Qori Solihah; Sumaryadi, Mas Yedi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 2 No 2 (2020): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2020.2.2.p147-155

Abstract

Background. Aims to determine and analyze the pregnancy rate of Etawah Crossbreed goats based on Non-return Rate method and Cubboni Reaction. Materials and methods. The materials used in this study were 20 Etawah Crossbreed goats, prostaglandins, urine, and concentrated H2SO4. The variables studied were the pregnancy rate of Etawah Crossbreed Goat based on Non-return Rate method and Cubboni Reaction. This study used an experimental method and the data obtained were processed using Chi Square analysis. Results. The analysis shows the number of x2<x2 tables (P>0.05), this means that the pregnancy detection method does not significantly affect the pregnancy rate of Etawah Crossbreed goats. Conclusion. Pregnancy rate of Etawah Crossbreed Goat based on Non-return Rate method amount 80% while based on Cubboni Reaction method amount 90%.
PENGARUH MACAM PENGENCER DAN LAMA PENYIMPANAN PADA SUHU 5oC TERHADAP MOTILITAS, VIABILITAS DAN ABNORMALITAS SPERMATOZOA AYAM KAMPUNG Rilandana, Dipta; Saleh, Dadang Mulyadi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2021.3.2.p184-191

Abstract

HUBUNGAN PANJANG, LINGKAR, DAN VOLUME SKROTUM DENGAN VIABILITAS DAN ABNORMALITAS SPERMATOZOA PADA KAMBING PEJANTAN PERANAKAN ETAWAH (PE) Pangestu, Mukti Santoso Aji; Sumaryadi, Mas Yedi; Nugroho, Aras Prasetiyo
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2021.3.3.p252-262

Abstract

HUBUNGAN ANTARA MORFOMETRIK DAN BOBOT BADAN DOMBA SAKUB JANTAN DI KABUPATEN BREBES Nurasih, Ari Dwi; Sumaryadi, Mas Yedi; Hidayah, Chomsiatun Nurul; Nugroho, Aras Prasetiyo; Yuwono, Pambudi; Haryoko, Imbang; Candrasari, Dewi Puspita
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (920.691 KB) | DOI: 10.20884/1.angon.2022.4.3.p285-290

Abstract

Background. Sakub sheep is a local sheep in Brebes Regency, Central Java, that has the potential as a genetic resource because it has a jumbo body. To know the body weight, weighing must be done. However, in buying and selling transactions, body weight can be estimated using livestock body measurements. Livestock body morphometrics such as body length, chest circumference, and shoulder height can be used to estimate livestock body weight. The study aimed to determine whether there is a relationship between body morphometric measures (body length, chest circumference, and shoulder height) and body weight in male sakub sheep. By studying this relationship, we can obtain useful information in optimizing ram production and can assist in determining appropriate management and nutrition strategies to increase ram body weight. Materials and Methods. Sheep were used in several age groups (12 months, 18 months, 24 months, and 36 months). The tools used in this study were hanging scales, measuring tapes, and stationery. Results. Based on the study's results, it can be seen that the relationship between body morphometrics (body length, chest circumference, and shoulder height) and body weight of male sakub sheep in various age groups has differences in the level of correlation. In the 12-month age group, the shoulder height variable had a very strong correlation (0.95) with body weight, while body length had a moderate correlation (0.55) and chest circumference had a low correlation (0.33). In the 18-month age group, the chest circumference variable had a very strong correlation (0.89) with body weight, while body length and shoulder height had strong correlations of 0.79 and 0.74, respectively. At 24 months of age, body length had a very strong correlation with body weight (0.99), while shoulder height had a moderate correlation (0.59) and chest circumference had a low correlation (0.29). In the 36-month age group, body length had a very strong correlation with body weight at 0.99, shoulder height had a strong correlation at 0.72, while the chest circumference variable had a low correlation (0.52). Conclusion. Based on the research and discussion that has been done, it can be concluded that there is a relationship between body morphometrics (body length, chest circumference, and shoulder height) to the body weight of male sakub sheep.
Co-Authors Aprila, Riska Dadang Mulyadi Saleh Dewi Puspita Candrasari Erman Huza, Ulil Albab Hidayah, Chomsiatun Nurul Hidayat, Nu'man Hidayat, Nu'man - Imbang Haryoko Ismoyowati, Ismoyowati - Krismiwati Muatip M. Y. Sumaryadi Mangliari, Yugys Nurasih, Ari Dwi Pambudi Yuwono Pangestu, Mukti Santoso Aji Pratiwi, Meyta Qori Solihah Rilandana, Dipta Siregar, Mitha Nurhafizhah Syamsi, Afduha Nurus